Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,426,694	Δ1 bp	5.4%	coding (243/942 nt)	PP_2127 ←	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,426,694	0	G	.	5.4%	296.7 / 10.4	92	coding (243/942 nt)	PP_2127	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (43/44);  new base . (2/3);  total (45/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

CGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGACCGTTGCCCAGCGCA  >  NC_002947/2426545‑2426839                                                                                                                                                        |                                                                                                                                                       cgcCGTGGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGAAGTTGACTGTTGGCGACGTGCGGTTCCCGTCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTg                                                                                                                                                        <  1:541455/150‑1 (MQ=255)  gcCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTgg                                                                                                                                                       >  1:2295688/1‑150 (MQ=255)   ccGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGgc                                                                                                                                                      >  1:1005918/1‑150 (MQ=255)   ccGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGgc                                                                                                                                                      >  1:1673023/1‑150 (MQ=255)     gTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGgcgc                                                                                                                                                    >  1:869552/1‑150 (MQ=255)      ttGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCgcc                                                                                                                                                   >  1:729578/1‑150 (MQ=255)          aGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCgcccagg                                                                                                                                               >  1:390051/1‑150 (MQ=255)            ccACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCgcccaggcc                                                                                                                                             <  2:2188871/150‑1 (MQ=255)              ccTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCgcccaggccca                                                                                                                                           <  2:1337295/149‑1 (MQ=255)              aCTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCCAGGTCGATCCACCT‑GCgcccggc                                                                                                                                               >  2:2198149/1‑143 (MQ=255)              aCTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCCAGGTCGATCCACCT‑GCgcccggc                                                                                                                                               <  1:2198149/146‑4 (MQ=255)                tGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGt                                                                                                                                         >  1:1103255/1‑150 (MQ=255)                 gCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGtt                                                                                                                                        <  2:2307958/150‑1 (MQ=255)                 gCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGtt                                                                                                                                        <  1:1963883/150‑1 (MQ=255)                    gTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTa                                                                                                                                     <  2:1568748/150‑1 (MQ=255)                       gCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTacaa                                                                                                                                  >  2:1311316/1‑150 (MQ=255)                       gCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCAAGGCCCAGTTGTacaa                                                                                                                                  >  2:1475216/1‑150 (MQ=255)                               gCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCAccc                                                                                                                          >  2:1765563/1‑150 (MQ=255)                                  gCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGc                                                                                                                       <  1:2170686/150‑1 (MQ=255)                                      ggAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTgg                                                                                                                   <  2:390051/149‑1 (MQ=255)                                          cAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGAc                                                                                                               >  2:2076103/1‑150 (MQ=255)                                          cAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGACGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGAc                                                                                                               <  1:692071/150‑1 (MQ=255)                                            gTTGACCGATGTCGTCGTGCAGTACCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑a                                                                                                         >  2:1184579/1‑150 (MQ=255)                                              tGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AAc                                                                                                       <  2:1103255/150‑1 (MQ=255)                                                  cGATGTCGTCGTGCAGTTCCCGGGCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTa                                                                                                                                     >  2:1185015/1‑120 (MQ=255)                                                  cGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTa                                                                                                                                     <  1:1185015/120‑1 (MQ=255)                                                  cGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTgca                                                                                                   >  1:1048581/1‑150 (MQ=255)                                                            gtGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACcagcag                                                                                        >  1:1814768/1‑150 (MQ=255)                                                             tGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAgg                                                                                       >  1:1929864/1‑150 (MQ=255)                                                             tGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAgg                                                                                       <  2:869552/150‑1 (MQ=255)                                                                aGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGc                                                                                                                       <  2:457302/120‑1 (MQ=255)                                                                aGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGc                                                                                                                       >  1:457302/1‑120 (MQ=255)                                                                  ttCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGc                                                                                  >  2:2431187/1‑150 (MQ=255)                                                                     ccGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCgcccaggccca                                                                                                                                           >  2:821718/1‑95 (MQ=255)                                                                     ccGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCgcccaggccca                                                                                                                                           <  1:821718/95‑1 (MQ=255)                                                                       ggCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACca                                                                                            <  2:821376/135‑1 (MQ=255)                                                                       ggCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACca                                                                                            >  1:821376/1‑135 (MQ=255)                                                                         cccAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGAtt                                                                           >  1:1080315/1‑150 (MQ=255)                                                                           cAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGAtt                                                                           >  2:812512/1‑148 (MQ=255)                                                                           cAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGAtt                                                                           <  1:812512/148‑1 (MQ=255)                                                                           cAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGc                                                                         >  2:2199400/1‑150 (MQ=255)                                                                              aTGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTg                                                                      <  1:706231/150‑1 (MQ=255)                                                                              aTGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTg                                                                      <  2:729578/150‑1 (MQ=255)                                                                               tGCTGGCGCTCGTCTTCTTGCACCTTGGGCATGCGCTCGGCC‑AGCGCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCGCAGGCCCAGTTGTACAACAGGACGCAGCGGGGCGACC‑‑‑‑AACTGCA‑GCGCCAGCAGGCTGGCCGGGATTGCCTgg                                                                     <  1:2131234/150‑1 (MQ=255)                                                                                 cTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGtt                                                                   <  2:1814768/150‑1 (MQ=255)                                                                                 cTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGtt                                                                   <  1:1765563/150‑1 (MQ=255)                                                                                 cTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGtt                                                                   <  2:507237/150‑1 (MQ=255)                                                                                    gcgcTCGTCTTATTGCAACTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGc                                                                >  2:524123/1‑150 (MQ=255)                                                                                     cgcTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGcc                                                               <  1:1010553/150‑1 (MQ=255)                                                                                       cTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGa                                                                             >  2:1089937/1‑134 (MQ=255)                                                                                       cTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGa                                                                             <  1:1089937/134‑1 (MQ=255)                                                                                       cTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAg                                                             >  2:2284969/1‑150 (MQ=255)                                                                                       cTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAg                                                             >  1:929206/1‑150 (MQ=255)                                                                                           tcttctTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGTCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTAc                                                         >  2:929963/1‑150 (MQ=255)                                                                                            cttcttGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACa                                                        <  1:358009/150‑1 (MQ=255)                                                                                             ttcttGCACCTTGAGAATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGtt                                                                   >  2:1177149/1‑138 (MQ=255)                                                                                             ttcttGCACCTTGAGAATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGtt                                                                   >  2:1177204/1‑138 (MQ=255)                                                                                             ttcttGCACCTTGAGAATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGtt                                                                   <  1:1177204/138‑1 (MQ=255)                                                                                             ttcttGCACCTTGAGAATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGtt                                                                   <  1:1177149/138‑1 (MQ=255)                                                                                              tcttGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGc                                                                                                                  <  1:1967526/95‑1 (MQ=255)                                                                                              tcttGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGc                                                                                                                  >  2:1967526/1‑95 (MQ=255)                                                                                              tcttGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAgc                                                      <  1:2431187/150‑1 (MQ=255)                                                                                              tcttGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAgc                                                      <  2:1945808/150‑1 (MQ=255)                                                                                                  gCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCa                                                  <  1:111160/150‑1 (MQ=255)                                                                                                     ccTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGt                                               >  1:206316/1‑150 (MQ=255)                                                                                                      cTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGtt                                              >  2:715772/1‑150 (MQ=255)                                                                                                      cTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGtt                                              <  1:2199400/150‑1 (MQ=255)                                                                                                       ttGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTg                                             <  1:2229379/150‑1 (MQ=255)                                                                                                       ttGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTg                                             <  2:1530093/150‑1 (MQ=255)                                                                                                       ttGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACAGCC‑GGTTCTGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTg                                             >  2:64137/1‑150 (MQ=255)                                                                                                       ttGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGACGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTg                                             <  2:713274/150‑1 (MQ=255)                                                                                                        tGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTgg                                            <  1:417664/150‑1 (MQ=255)                                                                                                              tGCGCCCGGCCGAGCT‑GGCCGAGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCa                                      >  1:988274/1‑150 (MQ=255)                                                                                                              tGCGCCCGGCCGAGCT‑GGCCGAGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCa                                      >  1:1701773/1‑150 (MQ=255)                                                                                                                  cTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGa                                  <  2:2237689/150‑1 (MQ=255)                                                                                                                   tCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAg                                 <  2:263212/150‑1 (MQ=255)                                                                                                                     ggCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGc                               <  2:1427474/150‑1 (MQ=255)                                                                                                                        c‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCAc                            <  2:761716/150‑1 (MQ=255)                                                                                                                        c‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCAc                            >  2:456383/1‑150 (MQ=255)                                                                                                                          aGCTCGGCCGGGCG‑CAGGTGGATCAACCCGGCAGGCACACCCAGTTTTACAATAGCACCCCCCGTGGCGACCCCGTAATCGTACGCACCAAAACGC‑‑G‑CGGG‑‑TGCCTGGTTGGCCAGGTACAGCGCCAAGTTGGCAAGTTGTGAGGCCACg                           >  1:544567/1‑150 (MQ=255)                                                                                                                            gctggCCGAGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGca                         <  2:988274/147‑1 (MQ=255)                                                                                                                             tCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGcac                        >  1:608305/1‑150 (MQ=255)                                                                                                                               gcctgggCGCCAGGTCGATCCACCT‑GCGCCC‑GGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAgg                                                                                       >  2:550352/5‑84 (MQ=18)                                                                                                                               gcctgggCGCCAGGTCGATCCACCT‑GCGCCC‑GGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAgg                                                                                       <  1:550352/80‑1 (MQ=18)                                                                                                                                    ggcgccAGGTCGATCCACCT‑GCGCCC‑GGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGAc                <  1:1838665/145‑1 (MQ=255)                                                                                                                                    ggCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGa                 >  2:498559/1‑150 (MQ=255)                                                                                                                                          aGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGaataac            <  2:1005918/150‑6 (MQ=255)                                                                                                                                           ggTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGTCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGACCGTTg           <  1:929963/150‑1 (MQ=255)                                                                                                                                            gTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCTAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGACCGTTGc          >  2:1303449/1‑150 (MQ=255)                                                                                                                                            gTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGACCGTTGc          >  2:1572462/1‑150 (MQ=255)                                                                                                                                               gATCGACCTGGCGCTAATGTCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AGCGACA‑TCACCACGACTCGGGCCCGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGCCCACGCACAGCCCGACCGTTGCCCa       <  1:1475216/150‑1 (MQ=255)                                                                                                                                                    aCCTGGCGCCCAGGCTCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGACCGTTGCCCAGCgca  >  2:411569/1‑150 (MQ=255)                                                                                                                                                       tGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGACCGTTGc          >  1:1661839/1‑139 (MQ=255)                                                                                                                                                       tGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGACCGTTGc          <  2:1661839/139‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                       CGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCG‑CAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACC‑‑‑‑AACTGCA‑GCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCACGCACAGCCCGACCGTTGCCCAGCGCA  >  NC_002947/2426545‑2426839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑