Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,882,252:1 +A 6.2% coding (1290/1356 nt) uacT → uric acid permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,882,2521.A 6.2% 383.5 / 18.7 97coding (1290/1356 nt)uacTuric acid permease
Reads supporting (aligned to +/- strand):  ref base . (46/45);  new base A (2/4);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

CCGCAACCAGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGTCGCCCG  >  NC_002947/4882105‑4882401
                                                                                                                                                     |                                                                                                                                                     
ccgcAACCAGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑Gt                                                                                                                                                     >  2:1898570/1‑150 (MQ=255)
       cAGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGt                                                                                                                                                >  1:1964857/1‑148 (MQ=255)
       cAGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGt                                                                                                                                                <  2:1964857/148‑1 (MQ=255)
       cAGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGGGGATGGAGCCCATCACCCCAGGCGCCTTCTCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTc                                                                                                                                              >  1:2378574/1‑150 (MQ=255)
          cTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAACAGTAC‑GTTCAAc                                                                                                                                           <  1:2116682/150‑1 (MQ=255)
          cTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAACAGTAC‑GTTCAAc                                                                                                                                           <  2:1778291/150‑1 (MQ=255)
           tGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACa                                                                                                                                          >  1:2053318/1‑150 (MQ=255)
           tGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACa                                                                                                                                          >  2:446078/1‑150 (MQ=255)
           tGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTAGACGAAGCCCATCCCCCACAGTTGAAT‑GCTGTGTTCCACGGTTTTTGCCCTGGTGTTGAACAGTAC‑GTTCAACa                                                                                                                                          <  1:414869/150‑1 (MQ=255)
                gttgCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCtt                                                                                                                                     <  2:530517/150‑1 (MQ=255)
                   gCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGc                                                                                                                                  <  1:446078/150‑1 (MQ=255)
                            gTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCgg                                                                                                                                >  1:1317361/1‑143 (MQ=255)
                            gTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCgg                                                                                                                                <  2:1317361/143‑1 (MQ=255)
                            gTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGc                                                                                                                             >  2:1347143/1‑146 (MQ=255)
                            gTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGc                                                                                                                             <  1:1347143/146‑1 (MQ=255)
                             tCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGAcc                                                                                                                        >  1:706876/1‑150 (MQ=255)
                                 cTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCgcgc                                                                                                                    >  2:2452281/1‑150 (MQ=255)
                                  ttCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAACAGTAC‑GTTCAACa                                                                                                                                          >  2:20318/1‑127 (MQ=255)
                                  ttCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAACAGTAC‑GTTCAACa                                                                                                                                          <  1:20318/127‑1 (MQ=255)
                                  ttCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCa                                                                                                                   >  1:754463/1‑150 (MQ=255)
                                   tCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCaa                                                                                                                  <  1:2232292/150‑1 (MQ=255)
                                    cGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTAAACATCCTTGGCGGTGCCGACCGCGCAAt                                                                                                                 <  1:918145/150‑1 (MQ=255)
                                     gggTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATg                                                                                                                >  1:266602/1‑150 (MQ=255)
                                       gTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAc                                                                                                                                                        <  1:692758/109‑1 (MQ=255)
                                       gTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGca                                                                                                              <  1:1050712/150‑1 (MQ=255)
                                       gTTGATCCCCGTGGTGCGTCAGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAc                                                                                                                                                        >  2:692758/1‑109 (MQ=255)
                                          gATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACaa                                                                                                           <  1:1650689/150‑1 (MQ=255)
                                          gATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACaa                                                                                                           <  2:1745698/150‑1 (MQ=255)
                                          gATCCCCGTGGTGCGTCCGGAGTTCCTTGCACAGACGCCACGGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACaa                                                                                                           <  2:123423/150‑1 (MQ=255)
                                           aTCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAac                                                                                                          <  2:2378859/150‑1 (MQ=255)
                                             ccccGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGa                                                                                                                          <  1:496659/132‑1 (MQ=255)
                                             ccccGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGa                                                                                                                          >  2:496659/1‑132 (MQ=255)
                                               ccGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAacgacg                                                                                                      >  2:1478319/1‑150 (MQ=255)
                                               ccGTGGTACGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAacgacg                                                                                                      >  1:1311987/1‑150 (MQ=255)
                                                cGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGt                                                                                                     >  1:2298572/1‑150 (MQ=255)
                                                  tgctgCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCt                                                                                                   <  1:1416191/147‑1 (MQ=255)
                                                  gggtgCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCt                                                                                                   <  2:1475925/149‑1 (MQ=255)
                                                         tCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCa                                                                                            >  2:2383008/1‑150 (MQ=255)
                                                            ggAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCa                                                                                         >  1:335694/1‑150 (MQ=255)
                                                            ggAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGAATCGCCATG‑GCCACGGTCAAACCCCGATTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGGGCCGACCGCGCAATGCACAACGACGTGTGTCACCAGcg                                                                                         >  1:535098/1‑149 (MQ=255)
                                                             gAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCAt                                                                                        <  2:266602/150‑1 (MQ=255)
                                                              aGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCCGCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCAtt                                                                                       <  2:535098/150‑1 (MQ=255)
                                                                 tCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAg                                                                                    <  1:1898570/150‑1 (MQ=255)
                                                                   tttGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGcc                                                                                  >  2:760296/1‑150 (MQ=255)
                                                                   tttGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGcc                                                                                  >  2:137794/1‑150 (MQ=255)
                                                                    ttGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCg                                                                                                                                 >  1:2156858/1‑102 (MQ=255)
                                                                    ttGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCg                                                                                                                                 <  2:2156858/102‑1 (MQ=255)
                                                                       cccAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGTGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAgg                                                                              <  2:1167724/150‑1 (MQ=255)
                                                                       cccAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAgg                                                                              <  2:1284825/150‑1 (MQ=255)
                                                                        ccAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAggg                                                                             >  1:1587276/1‑150 (MQ=255)
                                                                        ccAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAggg                                                                             >  2:252260/1‑150 (MQ=255)
                                                                         cAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAgggg                                                                            >  1:1703980/1‑150 (MQ=255)
                                                                           gaggCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAgggggg                                                                          <  2:285808/147‑1 (MQ=255)
                                                                           gATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAgggggg                                                                          <  1:1478319/150‑1 (MQ=255)
                                                                               ccACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGt                                                                      <  1:1558884/150‑1 (MQ=255)
                                                                                cacaGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTTAACATCCTTGGCGGTGCCGACCGCGCCATGCACAACGACGTCTGTCACGAGCATTGAGCCAAGGGGGGCGGTg                                                                     >  1:1343318/1‑150 (MQ=255)
                                                                                cacaGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTg                                                                     >  1:1491991/1‑150 (MQ=255)
                                                                                 acaGTGGATGGAGCCAATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGc                                                                    >  2:394834/1‑150 (MQ=255)
                                                                                   aGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCAt                                                                  <  1:137794/150‑1 (MQ=255)
                                                                                      ggatggaGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGc                                                               <  2:754463/150‑1 (MQ=255)
                                                                                         tggaGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCAGTAGACGGTTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGc                                                            <  1:258090/150‑1 (MQ=255)
                                                                                           gaGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGccc                                                          <  2:2298572/150‑1 (MQ=255)
                                                                                              cccATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCg                                                                        <  2:1790927/133‑1 (MQ=255)
                                                                                              cccATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCg                                                                        >  1:1790927/1‑133 (MQ=255)
                                                                                              cccATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTc                                                       <  2:2031023/150‑1 (MQ=255)
                                                                                              cccATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTc                                                       <  2:706876/150‑1 (MQ=255)
                                                                                                cATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTACATGGCTGCCCCTCGt                                                     >  1:1304709/1‑150 (MQ=255)
                                                                                                 aTCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGtt                                                    <  2:978515/150‑1 (MQ=255)
                                                                                                    aCCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGAGCATGGCTGCCCCTCGtgccc                                                 >  1:97532/1‑146 (MQ=255)
                                                                                                        acaGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCaa                                                                                                                                            <  2:1800768/55‑1 (MQ=255)
                                                                                                        acaGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCaa                                                                                                                                            >  1:1800768/1‑55 (MQ=255)
                                                                                                           gCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCGCCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCa                                          <  2:743090/150‑1 (MQ=255)
                                                                                                               tATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTaa                                      >  1:2217405/1‑150 (MQ=255)
                                                                                                                aTCGCCATG‑GCCATGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAAc                                     <  2:710441/150‑1 (MQ=255)
                                                                                                                aTCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAAc                                     <  2:1587276/150‑1 (MQ=255)
                                                                                                                 tCGCCATG‑GCCACGGTCTGAGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCg                                                      <  1:101226/132‑1 (MQ=255)
                                                                                                                 tCGCCATG‑GCCACGGTCTGAGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCg                                                      >  2:101226/1‑132 (MQ=255)
                                                                                                                   gCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATgg                                                                <  2:233589/120‑1 (MQ=255)
                                                                                                                   gCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATgg                                                                >  1:233589/1‑120 (MQ=255)
                                                                                                                     cATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGa                                                                                     <  2:2371670/97‑1 (MQ=255)
                                                                                                                     cATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGa                                                                                     >  1:2371670/1‑97 (MQ=255)
                                                                                                                            cACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGt                          >  2:880052/1‑150 (MQ=255)
                                                                                                                                  cTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGa                                                                                     <  2:863807/85‑1 (MQ=255)
                                                                                                                                  cTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGa                                                                                     >  1:863807/1‑85 (MQ=255)
                                                                                                                                   tgtgCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACccg                   >  1:1199182/1‑150 (MQ=255)
                                                                                                                                     tgCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACccgcc                 >  1:874866/1‑150 (MQ=255)
                                                                                                                                       cccTGGTGTTGAAC‑GTACTGTGCAACAGCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCgg               >  2:1403240/1‑150 (MQ=255)
                                                                                                                                       caaggatgtTGAACAGTAC‑GTTCAACACCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGCGCGGTGCATGGCTGCCCCTCGCTCACCTCTGCAGTAACCGCTACCCCGTCCACCCGCCgg               >  2:518758/7‑150 (MQ=255)
                                                                                                                                        ccTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGa              >  1:1939550/1‑150 (MQ=255)
                                                                                                                                        ccTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGa              >  2:2021026/1‑150 (MQ=255)
                                                                                                                                         cTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGaa             <  2:2053318/150‑1 (MQ=255)
                                                                                                                                          tGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCa                                          >  2:539255/1‑120 (MQ=255)
                                                                                                                                          tGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCa                                          <  1:539255/120‑1 (MQ=255)
                                                                                                                                          tGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAAt            <  2:874866/150‑1 (MQ=255)
                                                                                                                                          tGGNGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAAt            >  2:1516874/1‑150 (MQ=255)
                                                                                                                                            gtgtTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATgg                                                                <  1:2450863/96‑1 (MQ=255)
                                                                                                                                            gtgtTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATgg                                                                >  2:2450863/1‑96 (MQ=255)
                                                                                                                                              gtTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGACTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGt        <  2:1311987/150‑1 (MQ=255)
                                                                                                                                                 gAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGTCGc     >  2:2246585/1‑150 (MQ=255)
                                                                                                                                                    c‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGTCGCCCg  >  2:2311491/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGCAACCAGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATG‑GCCACGGTCTGTGCCCTGGTGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGTCGCCCG  >  NC_002947/4882105‑4882401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑