BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	428,861:1	+C	11.2%	coding (149/957 nt)	PP_0351 ←	transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	428,861	1	.	C	11.2%	374.8 / 46.4	107	coding (149/957 nt)	PP_0351	transmembrane sensor
Reads supporting (aligned to +/- strand): ref base . (39/56); new base C (6/6); total (45/62)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

Only 100 of 111 total aligned reads displayed.

CCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAGGTTGCTC  >  NC_002947/428713‑429007

 cATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑gg                                                                                                                                                  <  2:1333089/150‑1 (MQ=255)

 cATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑gg                                                                                                                                                  >  1:2035242/1‑150 (MQ=255)

 cATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑gg                                                                                                                                                  <  2:1764477/150‑1 (MQ=255)

  aTGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑Ggc                                                                                                                                                 <  1:1186770/150‑1 (MQ=255)

     ccAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCC‑AGCTCGGCGcc                                                                                                                                              <  1:903519/150‑1 (MQ=255)

       agcagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAg                                                                                                                                            <  1:1201317/150‑1 (MQ=255)

       agcagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAg                                                                                                                                            <  2:1524239/150‑1 (MQ=255)

       agcagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCCATTGGTGCAGCGAATGGCTGGCCGACCCAGCGTCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCaa                                                                                                                                            >  1:876727/1‑149 (MQ=255)

        gcagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAgg                                                                                                                                           >  1:2199914/1‑150 (MQ=255)

         cagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGc                                                                                                                                          <  2:1788233/150‑1 (MQ=255)

          agcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGTCTTCCTCGTGATTTCACCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCa                                                                                                                                         <  1:1124184/150‑1 (MQ=255)

           gcgcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCCTGGCGCC‑AGCTCGGCGCCatggac                                                                                                                                        <  2:50603/150‑6 (MQ=255)

           gcgcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAc                                                                                                                                        <  2:1049111/150‑1 (MQ=255)

             gcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgt                                                                                                                                      <  2:1892801/150‑1 (MQ=255)

              cgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtg                                                                                                                                     >  2:1359651/1‑150 (MQ=255)

                 cGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgcgt                                                                                                                                  >  1:844224/1‑150 (MQ=255)

                  gAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgcgtg                                                                                                                                 <  1:836183/150‑1 (MQ=255)

                      aGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGctct                                                                                                                             <  2:2121712/150‑1 (MQ=255)

                       gAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTTGTTTGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCtt                                                                                                                            >  1:723368/1‑150 (MQ=255)

                       gAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGAACTGGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCtt                                                                                                                            >  1:1308070/1‑150 (MQ=255)

                        aaaCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTc                                                                                                                           <  2:656595/150‑1 (MQ=255)

                        aaaCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTc                                                                                                                           <  2:1390103/150‑1 (MQ=255)

                        aaaCGTGCCGGCCAGCCCGCCGCAAATCTTCTGAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTc                                                                                                                           >  1:663432/1‑150 (MQ=255)

                            gTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTg                                                                                                                       >  1:1648782/1‑150 (MQ=255)

                              gCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTggc                                                                                                                     >  2:1397903/1‑150 (MQ=255)

                                 ggCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTggcggc                                                                                                                  >  2:403741/1‑150 (MQ=255)

                                 ggCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCC‑AGCTCGGCGcc                                                                                                                                              >  1:1582613/1‑122 (MQ=255)

                                 ggCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCC‑AGCTCGGCGcc                                                                                                                                              <  2:1582613/122‑1 (MQ=255)

                                    cAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGTCGCGCGATTGCAGCTGGACCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCTTGCTCTTCGCTGGCGGCATg                                                                                                               <  1:1206690/150‑1 (MQ=255)

                                       cccGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCa                                                                                                            <  1:1835632/150‑1 (MQ=255)

                                       cccGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCa                                                                                                            <  1:544986/150‑1 (MQ=255)

                                        ccgccgCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAg                                                                                                           >  1:2444672/1‑150 (MQ=255)

                                        ccgccgCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAg                                                                                                           >  2:56897/1‑150 (MQ=255)

                                             gcaaaCCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCAt                                                                                                      <  2:630577/147‑1 (MQ=255)

                                              ccAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCCGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATg                                                                                                     >  2:308800/1‑150 (MQ=255)

                                                aaCCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGag                                                                                                   >  1:962797/1‑149 (MQ=255)

                                                aaCCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGaa                                                                                                   >  1:1812373/1‑150 (MQ=255)

                                                aaCCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCC‑AGCTCGGCGCCatg                                                                                                                                           <  1:1491661/110‑3 (MQ=255)

                                                aaCCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCC‑AGCTCGGCGCCatg                                                                                                                                           >  2:1491661/1‑108 (MQ=255)

                                                   cTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCCTGAATTc                                                                                                >  2:2406109/1‑150 (MQ=255)

                                                    ttGCCAATGCGTCGCCGCAGGCTGACGCGCGATTGCAGCGGAGTTTTTCTTGACTGGCCAGCTGCCGCTGAAACGCGGTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCg                                                                                               >  1:1003312/1‑150 (MQ=255)

                                                     tGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGt                                                                                              <  1:314532/150‑1 (MQ=255)

                                                        cAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGc                                                                                           >  2:2302598/1‑150 (MQ=255)

                                                         aaTGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgt                                                                                                                                      <  1:2267181/106‑1 (MQ=255)

                                                         aaTGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgt                                                                                                                                      >  2:2267181/1‑106 (MQ=255)

                                                         aaTGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCg                                                                                               <  2:790411/145‑1 (MQ=255)

                                                         aaTGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCg                                                                                               >  1:790411/1‑145 (MQ=255)

                                                              gTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTg                                                                                            >  2:501092/1‑143 (MQ=255)

                                                                 gccgcAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAg                                                                                  <  1:214868/150‑1 (MQ=255)

                                                                 gccgcAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAg                                                                                  >  2:588061/1‑150 (MQ=255)

                                                                   cgcAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGc                                                                                <  1:56897/150‑1 (MQ=255)

                                                                    gcAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGACGGGCCAGCTGCCGCGCTAACGCGTTAGTAGATGGCGGCGAGCT‑GGCGCCATGCATGGGGGTGCTCTTCTCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCg                                                                               >  2:865269/1‑150 (MQ=255)

                                                                          tGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCgc                                                                         >  2:86506/1‑150 (MQ=255)

                                                                          tGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCgc                                                                         <  1:1509255/150‑1 (MQ=255)

                                                                              gcgcgATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGc                                                                            <  2:2223412/143‑1 (MQ=255)

                                                                              gcgcgATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGc                                                                            >  1:2223412/1‑143 (MQ=255)

                                                                              gcgcgATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCgctgct                                                                     >  2:2094151/1‑150 (MQ=255)

                                                                              gcgcgATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCgctgct                                                                     >  2:824472/1‑150 (MQ=255)

                                                                                 cgATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCtctc                                                                  <  2:557379/150‑1 (MQ=255)

                                                                                 cgATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCtctc                                                                  <  2:2035242/150‑1 (MQ=255)

                                                                                  gATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCgctgctgagc                                                                 <  2:1787671/150‑5 (MQ=255)

                                                                                      gcagcaGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCaa                                                             <  1:2013115/150‑1 (MQ=255)

                                                                                       cagcagCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAg                                                            >  2:2131529/1‑150 (MQ=255)

                                                                                        agcagcGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGTCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGTTCTCGCCAAgg                                                           >  1:765541/1‑150 (MQ=255)

                                                                                         gcagcGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGc                                                          <  1:1359651/150‑1 (MQ=255)

                                                                                          cagcGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCt                                                         <  1:2094151/150‑1 (MQ=255)

                                                                                            gcgcCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCGTGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGa                                                       <  1:782688/150‑1 (MQ=255)

                                                                                             cgcCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTc                                                                                                                           >  1:252134/1‑81 (MQ=255)

                                                                                              gcCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGa                                                       <  2:1288474/148‑1 (MQ=255)

                                                                                              gcCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGa                                                       >  1:1288474/1‑148 (MQ=255)

                                                                                                    cGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAg                                               <  1:444647/150‑1 (MQ=255)

                                                                                                           ggCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCtt                                                                                                                            <  2:1853450/66‑1 (MQ=255)

                                                                                                           ggCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCtt                                                                                                                            >  1:1853450/1‑66 (MQ=255)

                                                                                                             ccAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATcgcg                                      <  1:1098505/150‑1 (MQ=255)

                                                                                                              cAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCgg                                     >  1:400044/1‑150 (MQ=255)

                                                                                                              cAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCgg                                     <  2:2097921/150‑1 (MQ=255)

                                                                                                                  tggcgcgCTAACGCGTTGGGCTATGGCGCCGAGCT‑GACGTGTGTCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTc                                 <  1:24026/147‑1 (MQ=255)

                                                                                                                  tGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTc                                 <  1:612611/150‑1 (MQ=255)

                                                                                                                    ccGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCa                               <  2:1211636/150‑1 (MQ=255)

                                                                                                                    ccGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCCGCATGCCAGCGCATGAATTCGTTGCGTGCATCGGGCGTGACTCGGCGCTCGCCAAAGCTGAGTTTCCGGGCAATCGGTGCGTCCa                               <  2:25898/150‑1 (MQ=255)

                                                                                                                     cgcgCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTCCCAGGCAATCGCGGCTTCCAg                              >  1:449634/1‑150 (MQ=255)

                                                                                                                           aaCGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCg                        <  1:403741/150‑1 (MQ=255)

                                                                                                                            aCGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCgg                       <  2:844224/150‑1 (MQ=255)

                                                                                                                             cgcgTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCggg                      >  1:2359883/1‑150 (MQ=255)

                                                                                                                              gcgTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGa                     <  2:867995/150‑1 (MQ=255)

                                                                                                                              gcgTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGa                     >  2:1668766/1‑150 (MQ=255)

                                                                                                                                 ttGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGa                  <  2:723368/150‑1 (MQ=255)

                                                                                                                                    gttcctGGCGCC‑AGCTCGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCt                                                         >  2:1573667/6‑108 (MQ=255)

                                                                                                                                    gttcctGGCGCC‑AGCTCGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCt                                                         <  1:1573667/103‑1 (MQ=255)

                                                                                                                                      ccATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCCGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCAACAGGCGTGCCGGTGATCGCGCGAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCg             <  2:1915809/150‑1 (MQ=255)

                                                                                                                                        ctGGCGCC‑AGCTCGGCGCCAGGCACGTGGGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGGTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCggg           >  1:937100/2‑150 (MQ=255)

                                                                                                                                        ctGGCGCC‑AGCTCGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAg                                                            <  2:374238/100‑1 (MQ=255)

                                                                                                                                        ctGGCGCC‑AGCTCGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAg                                                            >  1:374238/2‑101 (MQ=255)

                                                                                                                                        aTGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCgc                                                                         <  2:1360766/88‑1 (MQ=255)

                                                                                                                                        aTGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCgc                                                                         >  1:1360766/1‑88 (MQ=255)

                                                                                                                                        aTGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCggg           >  2:1055059/1‑150 (MQ=255)

                                                                                                                                         tGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGa          <  1:1668766/150‑1 (MQ=255)

                                                                                                                                         tGGCGCC‑AGCTCGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCACGGCTGCCAGGACCCGGGACGAAACCGGGa          >  1:960815/1‑150 (MQ=255)

                                                                                                                                          ggCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAg         <  1:468483/150‑1 (MQ=255)

                                                                                                                                            cgcCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAGGt       <  1:515224/150‑1 (MQ=255)

CCATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAGGTTGCTC  >  NC_002947/428713‑429007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`