Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,459,977 A→T 6.0% L143Q (CTG→CAG)  PP_3075 ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,459,9770AT 6.0% 303.5 / 5.7 97L143Q (CTG→CAG) PP_3075transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (34/57);  new base T (4/2);  total (38/59)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTCGTTTGGCTTCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTCACCACATCGCGCATCAG  >  NC_002947/3459829‑3460125
                                                                                                                                                    |                                                                                                                                                    
gcgTCGTTTGGCTTCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCCGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAg                                                                                                                                                     <  1:1630198/150‑1 (MQ=255)
 cgtcgtTTGGCTTCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGt                                                                                                                                                    <  2:1092211/150‑1 (MQ=255)
     gtTTGGCTTCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTtcgt                                                                                                                                                <  1:1686207/150‑1 (MQ=255)
      tttGGCTTCCAGGCTGGGTGCTCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTtcgtc                                                                                                                                               <  2:2274843/150‑1 (MQ=255)
      tttGGCTTCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTtcgtc                                                                                                                                               <  2:1237551/150‑1 (MQ=255)
      tttGGCTTCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTtcgtc                                                                                                                                               <  1:760500/150‑1 (MQ=255)
      tttGGCTTCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTtcgtc                                                                                                                                               <  1:2265987/150‑1 (MQ=255)
          gCTTCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAAc                                                                                                                                           <  2:182242/150‑1 (MQ=255)
            ttCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAg                                                                                                                                         >  2:374870/1‑150 (MQ=255)
            ttCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAg                                                                                                                                         >  1:1491118/1‑150 (MQ=255)
             tCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAgc                                                                                                                                        <  2:465323/150‑1 (MQ=255)
             tCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAgc                                                                                                                                        <  1:784471/150‑1 (MQ=255)
               cAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAgcgc                                                                                                                                      >  2:296624/1‑150 (MQ=255)
                aGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCTGTTCGACGAACTGCGCa                                                                                                                                     <  1:809627/150‑1 (MQ=255)
                  gCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCaaa                                                                                                                                   >  1:472740/1‑150 (MQ=255)
                    tGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGc                                                                                                                                 <  2:53361/150‑1 (MQ=255)
                       gcgcgcTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCaa                                                                                                                              <  2:227121/150‑1 (MQ=255)
                       gcgcgcTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCaa                                                                                                                              >  2:2375456/1‑150 (MQ=255)
                         gcgcTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATg                                                                                                                            <  1:1856185/150‑1 (MQ=255)
                         gcgcTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATg                                                                                                                            <  1:851102/150‑1 (MQ=255)
                         gcgcTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATg                                                                                                                            >  1:1258951/1‑150 (MQ=255)
                          cgcTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATgg                                                                                                                           <  2:310024/150‑1 (MQ=255)
                             tgctgcCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGAAGTACGTCGAACAGCGCAAAGCCAATGgcgc                                                                                                                        >  1:1183399/1‑150 (MQ=255)
                                tgcCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGa                                                                                                                     >  2:1979403/1‑150 (MQ=255)
                                 gcCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGAt                                                                                                                    >  1:2145104/1‑150 (MQ=255)
                                             ggCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTg                                                                                                        <  2:1523350/150‑1 (MQ=255)
                                             ggCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTg                                                                                                        <  2:1081730/150‑1 (MQ=255)
                                             ggCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTg                                                                                                        >  2:2278397/1‑150 (MQ=255)
                                                  aGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCGTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCCTCGAACAGCGCAACGCCAATGGCGCCGATCAGCGCGCCCTGGTCAt                                                                                                   <  2:571010/150‑1 (MQ=255)
                                                    cTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCg                                                                                                 >  2:1532712/1‑150 (MQ=255)
                                                         tttGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGAt                                                                                            <  1:950320/150‑1 (MQ=255)
                                                         tttGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGAt                                                                                            <  1:132280/150‑1 (MQ=255)
                                                           tGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATgg                                                                                          <  1:332751/150‑1 (MQ=255)
                                                               cTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAg                                                                                      >  1:779780/1‑150 (MQ=255)
                                                                     ggcgCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCAt                                                                                <  1:1047913/150‑1 (MQ=255)
                                                                     ggcgCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCAt                                                                                <  1:326629/150‑1 (MQ=255)
                                                                       cgcgcAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGa                                                                              <  2:782920/150‑1 (MQ=255)
                                                                       cgcgcAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGa                                                                              >  1:1788179/1‑150 (MQ=255)
                                                                       cgcgcAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCAGGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAGTCCACTCGCGTCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGa                                                                              <  2:1046790/150‑1 (MQ=255)
                                                                        gcgcAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGac                                                                             <  1:51730/150‑1 (MQ=255)
                                                                         cgcAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGacc                                                                            <  1:434092/150‑1 (MQ=255)
                                                                           taGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGaccac                                                                          <  1:2061845/149‑1 (MQ=255)
                                                                            aGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGAt                                                                                            >  1:1627173/1‑131 (MQ=255)
                                                                            aGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGAt                                                                                            <  2:1627173/131‑1 (MQ=255)
                                                                            aGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGaccacc                                                                         >  1:2320275/1‑150 (MQ=255)
                                                                              ttGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTAACGAACACGCAACACACAGCGCTGAGAGCGGAAGAGCGCAGTGCCGGGGGCGCGTGTGGGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCaa                                                                       >  2:1463524/1‑150 (MQ=255)
                                                                               tGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGAGACGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAAc                                                                      <  2:690726/150‑1 (MQ=255)
                                                                                gCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACg                                                                     <  2:779780/150‑1 (MQ=255)
                                                                                  gAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACGACCAACGGc                                                                   >  1:363392/1‑150 (MQ=255)
                                                                                    gCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTc                                                                 <  2:472740/150‑1 (MQ=255)
                                                                                        gTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTc                                                             <  1:316462/150‑1 (MQ=255)
                                                                                            aGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTgg                                                                                              >  2:469450/1‑113 (MQ=255)
                                                                                            aGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTgg                                                                                              <  1:469450/113‑1 (MQ=255)
                                                                                             ggCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCgt                                                        <  2:2145104/150‑1 (MQ=255)
                                                                                              gCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCgtg                                                       >  2:179348/1‑150 (MQ=255)
                                                                                              gCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCgtg                                                       >  2:779184/1‑150 (MQ=255)
                                                                                               ccAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCgtgt                                                      <  2:112351/150‑1 (MQ=255)
                                                                                               ccAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCgtgt                                                      <  2:803958/150‑1 (MQ=255)
                                                                                                cAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGt                                                                                                <  2:1549180/107‑1 (MQ=255)
                                                                                                cAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGt                                                                                                >  1:1549180/1‑107 (MQ=255)
                                                                                                    tCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGc                                                 <  2:680532/150‑1 (MQ=255)
                                                                                                    tCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGc                                                 <  2:2245524/150‑1 (MQ=255)
                                                                                                         ctgcATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCCTGACCACCACCGCCTCGTGCGGCGTGTGCAGCATGTc                                            >  2:380757/1‑150 (MQ=255)
                                                                                                         ctgcATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTc                                            <  1:179348/150‑1 (MQ=255)
                                                                                                         ctgcATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTc                                            <  2:1762455/150‑1 (MQ=255)
                                                                                                           gcATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCAt                                               >  2:245708/1‑145 (MQ=255)
                                                                                                           gcATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCAt                                               <  1:245708/145‑1 (MQ=255)
                                                                                                           gcATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCca                                          <  1:713417/150‑1 (MQ=255)
                                                                                                            cATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCcag                                         >  1:210711/1‑150 (MQ=255)
                                                                                                            cATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCcag                                         >  1:159406/1‑150 (MQ=255)
                                                                                                              tGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCATCGTGATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCcagca                                       <  2:1786357/150‑1 (MQ=255)
                                                                                                                 tGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGa                                    >  2:877476/1‑150 (MQ=255)
                                                                                                                 tGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGa                                    >  1:1809626/1‑150 (MQ=255)
                                                                                                                 tGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCACTGGTCCTCGTGGATGGACTGACGCAGGACCACCAGCGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGa                                    <  1:647644/150‑1 (MQ=255)
                                                                                                                  ggCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCaa                                                                       <  2:2151176/114‑1 (MQ=255)
                                                                                                                  ggCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCaa                                                                       >  1:2151176/1‑114 (MQ=255)
                                                                                                                  ggCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGAt                                   >  1:1490334/1‑150 (MQ=255)
                                                                                                                  ggCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGAt                                   <  2:2320275/150‑1 (MQ=255)
                                                                                                                    cGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTTGAGCATGTCCAGCAGGATgg                                 <  1:2353656/150‑1 (MQ=255)
                                                                                                                     gTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATggg                                <  2:159406/150‑1 (MQ=255)
                                                                                                                      tAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATgg                                                                                          <  2:1943736/91‑1 (MQ=255)
                                                                                                                      tAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATgg                                                                                          >  1:1943736/1‑91 (MQ=255)
                                                                                                                          gcTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGt                                                                                                <  2:2034777/81‑1 (MQ=255)
                                                                                                                          gcTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGt                                                                                                >  1:2034777/1‑81 (MQ=255)
                                                                                                                              gAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGggccgg                            >  1:1966419/1‑145 (MQ=255)
                                                                                                                              gAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGggccgg                            <  2:1966419/145‑1 (MQ=255)
                                                                                                                                    cggcgACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTcac                 <  1:912521/150‑1 (MQ=255)
                                                                                                                                    cggcgACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTcac                 <  1:2260006/150‑1 (MQ=255)
                                                                                                                                             tttGCGCTGTTCGACGAACTGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGa                                                   >  2:113480/3‑107 (MQ=255)
                                                                                                                                             tttGCGCTGTTCGACGAACTGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGa                                                   <  1:113480/105‑1 (MQ=255)
                                                                                                                                             tttGCGCTGTTCGACGAACTGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTCACCACATcgcg        >  2:410988/3‑150 (MQ=255)
                                                                                                                                             tttGCGCTGTTCGACGAACTGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTCACCACATcgcg        >  1:2273357/3‑150 (MQ=255)
                                                                                                                                             gCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTCACCACATcgcg        >  2:781010/1‑150 (MQ=255)
                                                                                                                                             gCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTCACCACATcgcg        >  2:195351/1‑150 (MQ=255)
                                                                                                                                               tGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTCACCACATCGCGCa      >  1:2210977/1‑150 (MQ=255)
                                                                                                                                                 cgcAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTCACCACATCGCGCATc    <  2:1491118/150‑1 (MQ=255)
                                                                                                                                                   cAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTCACCACATCGCGCATCAg  <  2:1333123/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGTCGTTTGGCTTCCAGGCTGGGCGCGCTGCTGCCGACGAACTGGGCGAAGCTGTATTTGGCCTGGCGGGCGCGCAGTTGCGAGCGGGTCGAGGCCAGTTCCTGCTGCATGCTGGCGTAGCGCTTGAGCAGCGGCGACAGGCTGCGCAGTTCGTCGAACAGCGCAAAGCCAATGGCGCCGATCAGCGCGCCCTGGTCATCGTGGATGGGCAGGCGCATGACCACCAACGGCTCGTTCGGCGTGTCGAGCATGTCCAGCAGGATGGGCCGGCCATTGCTCACCACATCGCGCATCAG  >  NC_002947/3459829‑3460125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑