BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,542,361	A→G	7.2%	Y135Y (TAT→TAC)	galE ←	UDP‑glucose 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,542,361	0	A	G	7.2%	287.4 / 7.8	99	Y135Y (TAT→TAC)	galE	UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand): ref base A (47/44); new base G (4/3); total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 101 total aligned reads displayed.

CAGATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCG  >  NC_002947/3542214‑3542502

cAGATGACTCTCCGGCTCGACACGCCCGTCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATa                                                                                                                                             <  2:1098492/150‑1 (MQ=255)

      ggTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGCCAGCGCCCGCTGCATTGAAATATCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGtt                                                                                                                                       <  1:623449/150‑1 (MQ=255)

          tctggCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagc                                                                                                                                   <  1:1952752/147‑3 (MQ=255)

          gccGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCTGGTGGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATc                                                                                                                                   <  2:211332/149‑1 (MQ=255)

             ggCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGATTACCGAGTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGc                                                                                                                                >  2:1327446/1‑150 (MQ=255)

                tCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGcccc                                                                                                                             >  2:196123/1‑150 (MQ=255)

                  gTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCtt                                                                                                                           >  1:1548082/1‑150 (MQ=255)

                  gTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCtt                                                                                                                           >  1:453568/1‑150 (MQ=255)

                    gACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGATCAACCCGTATGGGTTGagcaaat                                                                                                                               <  2:1266106/144‑7 (MQ=255)

                    gACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGATCAACCCGTATGGGTTGagcaaat                                                                                                                               >  1:1266106/1‑138 (MQ=255)

                     aCGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgt                                                                                                                        <  1:2352689/150‑1 (MQ=255)

                         tCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgtgt                                                                                                                    <  2:416724/150‑1 (MQ=255)

                          cGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGtgtgtg                                                                                                                   <  1:1327446/150‑1 (MQ=255)

                           gCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgtgt                                                                                                                    >  1:521372/1‑148 (MQ=255)

                           gCCTAGCTGGCCTTCCGGGTCAGCGACCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgtgtgt                                                                                                                    <  2:521372/148‑1 (MQ=255)

                            ccTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCt                                                                                                                 >  2:909981/1‑150 (MQ=255)

                                gCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCat                                                                                                             <  2:1854692/150‑1 (MQ=255)

                                gCTGGCCTTCCGGGTCAGCGCCCGATGCATTGCACAAACTCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGCAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCat                                                                                                             >  1:1380166/1‑150 (MQ=255)

                                 cTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATc                                                                                                                                   >  2:1869605/1‑127 (MQ=255)

                                 cTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATc                                                                                                                                   <  1:1869605/127‑1 (MQ=255)

                                   ggCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGG‑CCCTTGGTGTGTGCTTCatcgat                                                                                                         <  1:1039946/150‑1 (MQ=255)

                                     ccTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatc                                                                                                        <  1:1704980/150‑1 (MQ=255)

                                      cTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatcg                                                                                                       <  2:1578238/150‑1 (MQ=255)

                                      cTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatcg                                                                                                       >  2:2121094/1‑150 (MQ=255)

                                      cTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatcg                                                                                                       >  1:1251982/1‑150 (MQ=255)

                                        tCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGc                                                                                                     <  2:1101478/150‑1 (MQ=255)

                                          cGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCCACGGCTTGGTGTGTGCTTCATCGATCGGCAc                                                                                                   <  1:1881503/150‑1 (MQ=255)

                                            ggTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACgg                                                                                                                                          <  2:1305657/109‑1 (MQ=255)

                                            ggTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACgg                                                                                                                                          >  1:1305657/1‑109 (MQ=255)

                                                aGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTg                                                                                             <  1:2400108/150‑1 (MQ=255)

                                                 gcgcCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatc                                                                                                        >  1:171806/1‑138 (MQ=255)

                                                 gcgcCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatc                                                                                                        <  2:171806/138‑1 (MQ=255)

                                                        cTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTAAAGAATCTGCTCGACCATCCATTTGCTCACCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGc                                                                                     <  1:26738/150‑1 (MQ=255)

                                                         tGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGcc                                                                                    <  1:306743/150‑1 (MQ=255)

                                                            aTTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGG‑CCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGtat                                                                                >  2:2176142/1‑150 (MQ=255)

                                                              ttgaaTAGGGCAAGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGtata                                                                               <  2:1317610/147‑1 (MQ=255)

                                                                 aaTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcgg                                                                            >  1:342493/1‑150 (MQ=255)

                                                                  aTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCTAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCTATCGGCACGTACTGCGGGTTGCCGTATAcggc                                                                           <  1:564601/150‑1 (MQ=255)

                                                                  aTAGCGCAGGCTTACCGACGTCAAACCATAGGGCCGGGCGAAGTCCTCAAGAATCTGCTCGAACATCCATTTGCTCAACCGTTGCTGGTTTGCCTGTCCCTTGTTGTGTGTTTCAGCGTTCGGCATGTACGGCGGGTTGCCGTATAcggc                                                                           <  2:272061/150‑1 (MQ=255)

                                                                   gaGCGCAGTCGTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcg                                                                          >  2:2241735/2‑150 (MQ=255)

                                                                    aGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACgg                                                                                                                                          >  2:1608228/1‑85 (MQ=255)

                                                                    aGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACgg                                                                                                                                          <  1:1608228/85‑1 (MQ=255)

                                                                           gCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCCTGCAACGGCGCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCga                                                                  >  2:919699/1‑150 (MQ=255)

                                                                           gCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCga                                                                  >  1:259857/1‑150 (MQ=255)

                                                                              tACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaaga                                                               <  1:1168436/150‑1 (MQ=255)

                                                                                 cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGa                                                                                                                                     >  1:2146017/1‑77 (MQ=255)

                                                                                 cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGa                                                                                                                                     <  2:2146017/77‑1 (MQ=255)

                                                                                 cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaaga                                                               <  1:1579728/147‑1 (MQ=255)

                                                                                 cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCgaaga                                                               >  2:1579728/1‑147 (MQ=255)

                                                                                 cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa                                                            >  1:460504/1‑150 (MQ=255)

                                                                                 cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa                                                            >  2:787098/1‑150 (MQ=255)

                                                                                 cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa                                                            >  1:2340127/1‑150 (MQ=255)

                                                                                   aCTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGACGAAAaca                                                          >  2:1144062/1‑150 (MQ=255)

                                                                                   aCTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAaca                                                          >  2:540195/1‑150 (MQ=255)

                                                                                      tCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCa                                                       <  1:196123/150‑1 (MQ=255)

                                                                                            cATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGcc                                                 >  1:1539596/1‑150 (MQ=255)

                                                                                             aTAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCCGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCt                                                <  2:1015427/150‑1 (MQ=255)

                                                                                                 gCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAac                                                           <  1:97488/135‑1 (MQ=255)

                                                                                                    cGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCg                                         <  2:342493/150‑1 (MQ=255)

                                                                                                       tCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCa                                      <  2:1548082/150‑1 (MQ=255)

                                                                                                         gAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCTGGTTGCCGTATACGGCGGCACTCGAAGAAAACACTAGGTGCCTGATGCCGGCAtt                                    >  1:1208274/1‑150 (MQ=255)

                                                                                                          aaGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTg                                   >  1:1919704/1‑150 (MQ=255)

                                                                                                               cTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCAt                              >  2:1776289/1‑150 (MQ=255)

                                                                                                               cTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCAt                              >  2:2276685/1‑150 (MQ=255)

                                                                                                                tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGCACGGACCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATg                             >  1:1879215/1‑150 (MQ=255)

                                                                                                                tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGa                                  >  2:920075/1‑145 (MQ=255)

                                                                                                                tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGa                                  <  1:920075/145‑1 (MQ=255)

                                                                                                                tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATg                             >  1:2204752/1‑150 (MQ=255)

                                                                                                                tCAAGAATCTGCTCGACCATCCATTTACTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATg                             >  2:2395796/1‑150 (MQ=255)

                                                                                                                tCAAGAAGCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATg                             <  2:502839/150‑1 (MQ=255)

                                                                                                                 cAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATgg                            <  1:2241735/150‑1 (MQ=255)

                                                                                                                  aaGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGc                           >  1:2006572/1‑150 (MQ=255)

                                                                                                                  aaGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGc                           >  1:395024/1‑150 (MQ=255)

                                                                                                                       tCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTgca                      >  2:526675/1‑150 (MQ=255)

                                                                                                                         tGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTgcagc                    >  1:1130543/1‑150 (MQ=255)

                                                                                                                           cTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGTGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGcagcag                  >  2:804313/1‑150 (MQ=255)

                                                                                                                           cTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGcagcag                  <  2:1130543/150‑1 (MQ=255)

                                                                                                                           cTCGACCATCCATTTGCTCAACCCATACGGCTTGCTCCGCGCTTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTTCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGcagcag                  <  2:1380166/150‑1 (MQ=255)

                                                                                                                            tCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGa                                  >  2:1604018/1‑133 (MQ=255)

                                                                                                                            tCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGa                                  <  1:1604018/133‑1 (MQ=255)

                                                                                                                            tCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAgg                 <  2:2204752/150‑1 (MQ=255)

                                                                                                                            tCGACCATCCATTTGCTCAACCCATACGCGCTGACAGGCGAACTGGCAGTTCATTGGCCCAGGGACACGGACTGTGTGTTGCCGTATAAGGCGACACTCATAGAAATCACCAGGTGCCTGATGCCTTCATTGACCATGGCCTGCAGCAgg                 >  1:1225170/1‑150 (MQ=255)

                                                                                                                              gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAg                                                      <  2:1699111/111‑1 (MQ=255)

                                                                                                                              gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAg                                                      >  1:1699111/1‑111 (MQ=255)

                                                                                                                                  atccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAgg           >  1:1797146/1‑150 (MQ=255)

                                                                                                                                   tccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAggg          <  1:1776289/150‑1 (MQ=255)

                                                                                                                                    ccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGTGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGt         <  1:804313/150‑1 (MQ=255)

                                                                                                                                    ccatTTACTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGt         <  1:2395796/150‑1 (MQ=255)

                                                                                                                                     catTTGCTCAATCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg        <  1:1077938/150‑1 (MQ=255)

                                                                                                                                      atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGTCCAATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTgg       <  1:2121094/150‑1 (MQ=255)

                                                                                                                                      atTTGCTCAACCCATACGGGCTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTgg       >  1:1210794/1‑150 (MQ=255)

                                                                                                                                          gatCAACCCGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGc   >  1:1569896/3‑150 (MQ=255)

                                                                                                                                          gatCAACCAGTATGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGc   >  2:1971646/3‑150 (MQ=255)

                                                                                                                                           cTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTAAACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCg  >  2:1046182/1‑150 (MQ=255)

                                                                                                                                                 ccATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTgca                      >  1:820241/1‑124 (MQ=255)

                                                                                                                                                 ccATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTgca                      <  2:820241/124‑1 (MQ=255)

                                                                                                                                                 ccATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTACCTGATGCCGGCATTGACCATGGCCTgcagc                    <  2:1675461/126‑1 (MQ=255)

                                                                                                                                                 ccATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTACCTGATGCCGGCATTGACCATGGCCTgcagc                    >  1:1675461/1‑126 (MQ=255)

                                                                                                                                                   aTACGGGTTGATCGGCCCCTTGGTGTGTGCTTCat                                                                                                             <  2:2201862/35‑1 (MQ=255)

                                                                                                                                                   aTACGGGTTGATCGGCCCCTTGGTGTGTGCTTCat                                                                                                             >  1:2201862/1‑35 (MQ=255)

CAGATGGGTTTCCGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCG  >  NC_002947/3542214‑3542502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`