Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,590,368	G→C	10.1%	R282R (CGG→CGC)	nicP‑I →	porin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,590,368	0	G	C	10.1%	274.1 / 15.6	95	R282R (CGG→CGC)	nicP‑I	porin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (46/37);  new base C (6/4);  total (53/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTA  >  NC_002947/3590222‑3590517                                                                                                                                                   |                                                                                                                                                      caaGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGTGTTCTTCTCGCTGTTCACTGCCCGGCAt                                                                                                                                                    >  2:696095/1‑150 (MQ=255)       tttGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCggg                                                                                                                                              >  1:2152729/1‑150 (MQ=255)            tgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAg                                                                                                                                         >  2:1008678/1‑150 (MQ=255)            tgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagtg                                                                                                                                         <  2:2152729/150‑4 (MQ=255)              gCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagt                                                                                                                                          <  2:472540/147‑3 (MQ=255)              gCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagt                                                                                                                                          >  1:472540/1‑145 (MQ=255)                 tGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTcc                                                                                                                                    >  2:1757429/1‑150 (MQ=255)                 tGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTcc                                                                                                                                    <  2:737262/150‑1 (MQ=255)                                ccGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGTCATGGCGTGCACAGCTTCCATTCCGGCTACCAgg                                                                                                                     >  2:723285/1‑150 (MQ=255)                                ccGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAgg                                                                                                                     >  2:2048858/1‑150 (MQ=255)                                 cGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAggg                                                                                                                    >  1:195236/1‑150 (MQ=255)                                        gACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTAt                                                                                                             >  2:110771/1‑150 (MQ=255)                                        gACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTAt                                                                                                             >  1:470112/1‑150 (MQ=255)                                          cTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATgg                                                                                                           <  1:1570672/150‑1 (MQ=255)                                             ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGa                                                                                                        >  1:1895005/1‑150 (MQ=255)                                             ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGa                                                                                                        >  1:1643454/1‑150 (MQ=255)                                               tACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATa                                                                                                      <  2:820187/150‑1 (MQ=255)                                                aCTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAg                                                                                                     <  2:1895005/150‑1 (MQ=255)                                                 cTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGc                                                                                                    <  2:2123530/150‑1 (MQ=255)                                                  tCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGca                                                                                                                                            <  1:2390674/109‑1 (MQ=255)                                                  tCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGca                                                                                                                                            >  2:2390674/1‑109 (MQ=255)                                                   cAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGccc                                                                                                  <  1:1757429/150‑1 (MQ=255)                                                    aGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCg                                                                                                 >  1:1045725/1‑150 (MQ=255)                                                        ccAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTcc                                                                                             >  2:2109343/1‑150 (MQ=255)                                                         cAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGc                                                                                                                                                 <  1:1345138/97‑1 (MQ=255)                                                         cAACCTGGGTTATTTCACCGCGCGTGAGCAAGACGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGc                                                                                                                                                 >  2:1345138/1‑97 (MQ=255)                                                          aaCCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCgg                                                                                                                                               <  1:2338217/98‑1 (MQ=255)                                                          aaCCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCgg                                                                                                                                               >  2:2338217/1‑98 (MQ=255)                                                             cTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCNCgg                                                                                        <  2:1517020/150‑1 (MQ=255)                                                             cTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACgg                                                                                        <  1:2048858/150‑1 (MQ=255)                                                             cTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACgg                                                                                        <  2:195236/150‑1 (MQ=255)                                                              tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCAt                                                                                                                                  >  1:2431789/1‑107 (MQ=255)                                                              tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCAt                                                                                                                                  >  1:2431790/1‑107 (MQ=255)                                                              tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCAt                                                                                                                                  <  2:2431789/107‑1 (MQ=255)                                                              tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCAt                                                                                                                                  <  2:2431790/107‑1 (MQ=255)                                                               gggTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGAATTTATGGCTATAGCCCGTTCCCACGGGt                                                                                      >  2:552853/1‑150 (MQ=255)                                                                ggTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTa                                                                                     >  2:1804660/1‑150 (MQ=255)                                                                 gTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTAt                                                                                    >  2:1672544/1‑150 (MQ=255)                                                                  ttATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTAtt                                                                                   <  2:301352/150‑1 (MQ=255)                                                                      ttCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGCTAGCCCGTTCCCACGGGTATTTGcc                                                                               >  1:232533/1‑150 (MQ=255)                                                                      ttCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCAGTTCCCCCGGGTATTTGcc                                                                               <  2:634128/150‑1 (MQ=255)                                                                        cACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCaa                                                                             >  2:1992611/1‑150 (MQ=255)                                                                             cgcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGc                                                                                                                            <  2:529094/98‑1 (MQ=255)                                                                             cgcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGc                                                                                                                            >  1:529094/1‑98 (MQ=255)                                                                              gcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGACATTGATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACAactc                                                                       <  1:552853/150‑1 (MQ=255)                                                                                gTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTAttt                                                                                  >  2:2095922/1‑137 (MQ=255)                                                                                gTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTAttt                                                                                  <  1:2095922/137‑1 (MQ=255)                                                                                gTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGc                                                                     >  2:115297/1‑150 (MQ=255)                                                                                    gCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGtt                                                                 <  2:470112/150‑1 (MQ=255)                                                                                      aaGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCGCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                                                               >  2:1238207/1‑150 (MQ=255)                                                                                           gATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTg                                                                >  2:81508/1‑144 (MQ=255)                                                                                           gATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTg                                                                <  1:81508/144‑1 (MQ=255)                                                                                            aTGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACg                                                         <  1:2109343/150‑1 (MQ=255)                                                                                                gctgctGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCGCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAggg                                                     >  1:521092/1‑149 (MQ=255)                                                                                                  tgctgGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGc                                                   <  1:110771/150‑1 (MQ=255)                                                                                                  tgctgGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCACGCCCTGACAACCACAACTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGc                                                   <  1:876712/150‑1 (MQ=255)                                                                                                     tgGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGa                                                >  1:1588421/1‑150 (MQ=255)                                                                                                      gggCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGAc                                               >  1:2207588/1‑150 (MQ=255)                                                                                                          aaCATCGACAACCAGGCGTTCTTCTCGCTTTTCACTGCCCGGCATGGCGGGCACATCTTACATGCCGGCTACCACGGCATTTATGACGATAGCCCATTCCCTCGGGTATTTCCCAACATTTCGCCGTTGGCCAACGAGGTGCCGACCTAt                                           <  2:607043/150‑1 (MQ=255)                                                                                                             aacgcaaaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGGTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAg                                        >  2:1271147/7‑150 (MQ=255)                                                                                                             aTCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCCGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAg                                        >  1:890312/1‑150 (MQ=255)                                                                                                                gACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAg                                                       >  2:76124/1‑132 (MQ=255)                                                                                                                gACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAg                                                       <  1:76124/132‑1 (MQ=255)                                                                                                                gACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGttt                                     >  1:1686811/1‑150 (MQ=255)                                                                                                                 aCAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTg                                    >  2:555590/1‑150 (MQ=255)                                                                                                                  cAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGc                                   <  2:1643454/150‑1 (MQ=255)                                                                                                                   aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCg                                                                                                                                                <  2:587280/40‑1 (MQ=255)                                                                                                                   aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCg                                                                                                                                                >  1:587280/1‑40 (MQ=255)                                                                                                                   aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGcc                                  >  2:2094876/1‑150 (MQ=255)                                                                                                                   aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGcc                                  <  2:2207588/150‑1 (MQ=255)                                                                                                                     ccAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTa                                <  2:2286631/150‑1 (MQ=255)                                                                                                                     ccAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTa                                <  1:1804660/150‑1 (MQ=255)                                                                                                                      cAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTac                               <  1:1672544/150‑1 (MQ=255)                                                                                                                       aGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTaca                              >  2:2026166/1‑150 (MQ=255)                                                                                                                        ggCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGCGGCCGACCTATGAGTTTGCCTacac                             >  2:554228/1‑150 (MQ=255)                                                                                                                              cttctCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACcgacga                       <  2:2190382/150‑1 (MQ=255)                                                                                                                                  tcGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGc                   >  1:224899/1‑150 (MQ=255)                                                                                                                                    gCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTc                 <  1:2094876/150‑1 (MQ=255)                                                                                                                                          cACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCa           >  1:1892181/1‑150 (MQ=255)                                                                                                                                          cACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCa           <  2:1892181/150‑1 (MQ=255)                                                                                                                                          cACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCa           >  2:105326/1‑150 (MQ=255)                                                                                                                                           tgtGCCCGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGcc                                  <  2:1325145/124‑1 (MQ=255)                                                                                                                                           tgtGCCCGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGcc                                  >  1:1325145/3‑126 (MQ=255)                                                                                                                                           tgtGCCCGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAg          >  2:1937432/3‑150 (MQ=255)                                                                                                                                           tgtGCCCGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAg          >  2:1340317/3‑150 (MQ=255)                                                                                                                                           aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGTCTACACCGACGAACGCTCGTa              <  2:2314512/146‑1 (MQ=255)                                                                                                                                           aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGTCTACACCGACGAACGCTCGTa              >  1:2314512/1‑146 (MQ=255)                                                                                                                                           aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAg          >  2:1030847/1‑150 (MQ=255)                                                                                                                                            cTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAgg         >  1:973064/1‑150 (MQ=255)                                                                                                                                             tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAgg         >  2:1175634/1‑149 (MQ=255)                                                                                                                                             tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAgg         <  1:1175634/149‑1 (MQ=255)                                                                                                                                             tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGt        <  1:1238207/150‑1 (MQ=255)                                                                                                                                              gCCCGACATGGCGGCCACAGCTTCCACGCCGGCTCCCACGACATTTAAGGAGATAACTACCTCCAGCCAGTATTTTCCATCATCTCGCCTTTGTGCAAAGAGGTGCCGACCTAAGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTa       <  1:168995/150‑1 (MQ=255)                                                                                                                                                ccGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACg     >  1:1058414/1‑150 (MQ=255)                                                                                                                                                ccGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACg     >  2:84106/1‑150 (MQ=255)                                                                                                                                                   gCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTa  >  1:832118/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                      CAAGGCTTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTA  >  NC_002947/3590222‑3590517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑