Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,590,373 G→C 9.4% G284A (GGC→GCC)  nicP‑I → porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,590,3730GC 9.4% 287.7 / 17.4 97G284A (GGC→GCC) nicP‑Iporin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (48/39);  new base C (6/3);  total (54/43)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

TTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTACG  >  NC_002947/3590228‑3590519
                                                                                                                                                 |                                                                                                                                                  
tttGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCggg                                                                                                                                                >  1:2152729/1‑150 (MQ=255)
     tgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAg                                                                                                                                           >  2:1008678/1‑150 (MQ=255)
     tgGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagtg                                                                                                                                           <  2:2152729/150‑4 (MQ=255)
       gCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagt                                                                                                                                            >  1:472540/1‑145 (MQ=255)
       gCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGCCATGCCGGGcagt                                                                                                                                            <  2:472540/147‑3 (MQ=255)
          tGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTcc                                                                                                                                      >  2:1757429/1‑150 (MQ=255)
          tGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTcc                                                                                                                                      <  2:737262/150‑1 (MQ=255)
                         ccGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGTCATGGCGTGCACAGCTTCCATTCCGGCTACCAgg                                                                                                                       >  2:723285/1‑150 (MQ=255)
                         ccGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAgg                                                                                                                       >  2:2048858/1‑150 (MQ=255)
                          cGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAggg                                                                                                                      >  1:195236/1‑150 (MQ=255)
                                 gACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTAt                                                                                                               >  2:110771/1‑150 (MQ=255)
                                 gACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTAt                                                                                                               >  1:470112/1‑150 (MQ=255)
                                   cTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATgg                                                                                                             <  1:1570672/150‑1 (MQ=255)
                                      ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGa                                                                                                          >  1:1643454/1‑150 (MQ=255)
                                      ggTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGa                                                                                                          >  1:1895005/1‑150 (MQ=255)
                                        tACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATa                                                                                                        <  2:820187/150‑1 (MQ=255)
                                         aCTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAg                                                                                                       <  2:1895005/150‑1 (MQ=255)
                                          cTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGc                                                                                                      <  2:2123530/150‑1 (MQ=255)
                                           tCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGca                                                                                                                                              >  2:2390674/1‑109 (MQ=255)
                                           tCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGca                                                                                                                                              <  1:2390674/109‑1 (MQ=255)
                                            cAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGccc                                                                                                    <  1:1757429/150‑1 (MQ=255)
                                             aGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCg                                                                                                   >  1:1045725/1‑150 (MQ=255)
                                                 ccAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTcc                                                                                               >  2:2109343/1‑150 (MQ=255)
                                                  cAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGc                                                                                                                                                   <  1:1345138/97‑1 (MQ=255)
                                                  cAACCTGGGTTATTTCACCGCGCGTGAGCAAGACGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGc                                                                                                                                                   >  2:1345138/1‑97 (MQ=255)
                                                   aaCCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCgg                                                                                                                                                 >  2:2338217/1‑98 (MQ=255)
                                                   aaCCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCgg                                                                                                                                                 <  1:2338217/98‑1 (MQ=255)
                                                      cTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCNCgg                                                                                          <  2:1517020/150‑1 (MQ=255)
                                                      cTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACgg                                                                                          <  2:195236/150‑1 (MQ=255)
                                                      cTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACgg                                                                                          <  1:2048858/150‑1 (MQ=255)
                                                       tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCAt                                                                                                                                    >  1:2431789/1‑107 (MQ=255)
                                                       tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCAt                                                                                                                                    >  1:2431790/1‑107 (MQ=255)
                                                       tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCAt                                                                                                                                    <  2:2431789/107‑1 (MQ=255)
                                                       tGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCAt                                                                                                                                    <  2:2431790/107‑1 (MQ=255)
                                                        gggTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGAATTTATGGCTATAGCCCGTTCCCACGGGt                                                                                        >  2:552853/1‑150 (MQ=255)
                                                         ggTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTa                                                                                       >  2:1804660/1‑150 (MQ=255)
                                                          gTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTAt                                                                                      >  2:1672544/1‑150 (MQ=255)
                                                           ttATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTAtt                                                                                     <  2:301352/150‑1 (MQ=255)
                                                               ttCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGCTAGCCCGTTCCCACGGGTATTTGcc                                                                                 >  1:232533/1‑150 (MQ=255)
                                                               ttCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCAGTTCCCCCGGGTATTTGcc                                                                                 <  2:634128/150‑1 (MQ=255)
                                                                 cACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCaa                                                                               >  2:1992611/1‑150 (MQ=255)
                                                                      cgcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGc                                                                                                                              >  1:529094/1‑98 (MQ=255)
                                                                      cgcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGc                                                                                                                              <  2:529094/98‑1 (MQ=255)
                                                                       gcgTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGACATTGATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACAactc                                                                         <  1:552853/150‑1 (MQ=255)
                                                                         gTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTAttt                                                                                    >  2:2095922/1‑137 (MQ=255)
                                                                         gTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTAttt                                                                                    <  1:2095922/137‑1 (MQ=255)
                                                                         gTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGc                                                                       >  2:115297/1‑150 (MQ=255)
                                                                             gCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGtt                                                                   <  2:470112/150‑1 (MQ=255)
                                                                               aaGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCGCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTgg                                                                 >  2:1238207/1‑150 (MQ=255)
                                                                                    gATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTg                                                                  <  1:81508/144‑1 (MQ=255)
                                                                                    gATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTg                                                                  >  2:81508/1‑144 (MQ=255)
                                                                                     aTGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACg                                                           <  1:2109343/150‑1 (MQ=255)
                                                                                         gctgctGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCGCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAggg                                                       >  1:521092/1‑149 (MQ=255)
                                                                                           tgctgGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGc                                                     <  1:110771/150‑1 (MQ=255)
                                                                                           tgctgGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCACGCCCTGACAACCACAACTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGc                                                     <  1:876712/150‑1 (MQ=255)
                                                                                              tgGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGa                                                  >  1:1588421/1‑150 (MQ=255)
                                                                                               gggCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGAc                                                 >  1:2207588/1‑150 (MQ=255)
                                                                                                   aaCATCGACAACCAGGCGTTCTTCTCGCTTTTCACTGCCCGGCATGGCGGGCACATCTTACATGCCGGCTACCACGGCATTTATGACGATAGCCCATTCCCTCGGGTATTTCCCAACATTTCGCCGTTGGCCAACGAGGTGCCGACCTAt                                             <  2:607043/150‑1 (MQ=255)
                                                                                                      aacgcaaaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGGTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAg                                          >  2:1271147/7‑150 (MQ=255)
                                                                                                      aTCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCCGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAg                                          >  1:890312/1‑150 (MQ=255)
                                                                                                         gACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAg                                                         <  1:76124/132‑1 (MQ=255)
                                                                                                         gACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAg                                                         >  2:76124/1‑132 (MQ=255)
                                                                                                         gACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGttt                                       >  1:1686811/1‑150 (MQ=255)
                                                                                                          aCAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTg                                      >  2:555590/1‑150 (MQ=255)
                                                                                                           cAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGc                                     <  2:1643454/150‑1 (MQ=255)
                                                                                                            aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCg                                                                                                                                                  <  2:587280/40‑1 (MQ=255)
                                                                                                            aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCg                                                                                                                                                  >  1:587280/1‑40 (MQ=255)
                                                                                                            aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGcc                                    >  2:2094876/1‑150 (MQ=255)
                                                                                                            aaCCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGcc                                    <  2:2207588/150‑1 (MQ=255)
                                                                                                              ccAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTa                                  <  1:1804660/150‑1 (MQ=255)
                                                                                                              ccAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTa                                  <  2:2286631/150‑1 (MQ=255)
                                                                                                               cAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTac                                 <  1:1672544/150‑1 (MQ=255)
                                                                                                                aGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTaca                                >  2:2026166/1‑150 (MQ=255)
                                                                                                                 ggCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGCGGCCGACCTATGAGTTTGCCTacac                               >  2:554228/1‑150 (MQ=255)
                                                                                                                       cttctCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACcgacga                         <  2:2190382/150‑1 (MQ=255)
                                                                                                                           tcGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGc                     >  1:224899/1‑150 (MQ=255)
                                                                                                                             gCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTc                   <  1:2094876/150‑1 (MQ=255)
                                                                                                                                   cACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCa             >  2:105326/1‑150 (MQ=255)
                                                                                                                                   cACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCa             >  1:1892181/1‑150 (MQ=255)
                                                                                                                                   cACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCa             <  2:1892181/150‑1 (MQ=255)
                                                                                                                                    tgtGCCCGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGcc                                    >  1:1325145/3‑126 (MQ=255)
                                                                                                                                    tgtGCCCGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGcc                                    <  2:1325145/124‑1 (MQ=255)
                                                                                                                                    tgtGCCCGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAg            >  2:1937432/3‑150 (MQ=255)
                                                                                                                                    tgtGCCCGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAg            >  2:1340317/3‑150 (MQ=255)
                                                                                                                                    aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGTCTACACCGACGAACGCTCGTa                <  2:2314512/146‑1 (MQ=255)
                                                                                                                                    aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGTCTACACCGACGAACGCTCGTa                >  1:2314512/1‑146 (MQ=255)
                                                                                                                                    aCTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAg            >  2:1030847/1‑150 (MQ=255)
                                                                                                                                     cTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAgg           >  1:973064/1‑150 (MQ=255)
                                                                                                                                      tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAgg           >  2:1175634/1‑149 (MQ=255)
                                                                                                                                      tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAgg           <  1:1175634/149‑1 (MQ=255)
                                                                                                                                      tGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGt          <  1:1238207/150‑1 (MQ=255)
                                                                                                                                       gCCCGACATGGCGGCCACAGCTTCCACGCCGGCTCCCACGACATTTAAGGAGATAACTACCTCCAGCCAGTATTTTCCATCATCTCGCCTTTGTGCAAAGAGGTGCCGACCTAAGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTa         <  1:168995/150‑1 (MQ=255)
                                                                                                                                         ccGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACg       >  1:1058414/1‑150 (MQ=255)
                                                                                                                                         ccGCCATGCCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACg       >  2:84106/1‑150 (MQ=255)
                                                                                                                                            gCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTa    >  1:832118/1‑150 (MQ=255)
                                                                                                                                             cATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACAACCAGACGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCAACACCGACGAACGCTCGTACCAGGTACGTTAc   <  1:1992611/150‑1 (MQ=255)
                                                                                                                                              aTGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTAGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTACg  >  1:412060/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTTGTTGGCCTGAAGCACAGCAAACCCGTCGGCGACTGGGTACTCAGTGCCAACCTGGGTTATTTCACCGCGCGTGAGCAAGGCGATGCGCTGCTGGGCAACATCGACAACCAGGCGTTCTTCTCGCTGTTCACTGCCCGGCATGGCGGGCACAGCTTCCATGCCGGCTACCAGGGCATTTATGGCGATAGCCCGTTCCCACGGGTATTTGCCAACATCTCGCCGTTGGGCAACGAGGTGCCGACCTATGAGTTTGCCTACACCGACGAACGCTCGTACCAGGTACGTTACG  >  NC_002947/3590228‑3590519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑