Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,667,708 G→A 6.4% A341V (GCC→GTC)  PP_3231 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,667,7080GA 6.4% 279.5 / 6.3 95A341V (GCC→GTC) PP_3231hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (43/45);  new base A (2/4);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GGACAACACATGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGAGCCCAG  >  NC_002947/3667560‑3667857
                                                                                                                                                     |                                                                                                                                                     
ggACAACACATGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATnggc                                                                                                                                                      <  2:1497415/150‑1 (MQ=255)
 gACAACACATGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATcggcg                                                                                                                                                     <  1:1597126/150‑1 (MQ=255)
      cacaTGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCggcggcggc                                                                                                                                                >  2:830152/1‑150 (MQ=255)
       acaTGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGCCACCGACCGCGGcc                                                                                                                                               >  2:1928595/1‑150 (MQ=255)
        caTGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCa                                                                                                                                              >  1:1542169/1‑150 (MQ=255)
         aTGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCAc                                                                                                                                             >  2:2449280/1‑150 (MQ=255)
          tGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCAcc                                                                                                                                            >  1:1813436/1‑150 (MQ=255)
            gcgcAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTg                                                                                                                                          >  1:669979/1‑150 (MQ=255)
            gcgcAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTg                                                                                                                                          <  1:1089946/150‑1 (MQ=255)
             cgcAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTgg                                                                                                                                         <  1:445904/150‑1 (MQ=255)
                   tCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGTGGCCACCTGGCCGcgg                                                                                                                                   >  1:466596/1‑150 (MQ=255)
                    ccTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGCCACCGACCGCGGCCAGGTGGCcgccgc                                                                                                                                  <  1:1929940/150‑4 (MQ=255)
                    ccTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGCCACCGACCGCGGCCAGGTGGCcgccgc                                                                                                                                  <  1:1928595/150‑4 (MQ=255)
                     cTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCACCGACCGCGGCCAGGTGGCcgccgcc                                                                                                                                 >  1:1735207/1‑146 (MQ=255)
                      ttGCGCTGTTGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcg                                                                                                                                <  1:100683/150‑1 (MQ=255)
                      ttGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGTCTGGCACGCCTGCGAGGCGGAACTGATCCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcg                                                                                                                                <  1:1106447/150‑1 (MQ=255)
                      ttGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGTCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACGTGGCCGcggtcg                                                                                                                                >  2:1330789/1‑150 (MQ=255)
                      ttGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcg                                                                                                                                >  2:1756849/1‑150 (MQ=255)
                        gcgcTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACTCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCACCGACCGCGGCCAGGTGGCcgccgccgat                                                                                                                              <  1:1196960/150‑8 (MQ=255)
                        gcgcTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCACCGACCGCGGCCAGGTGGCcgccgccgat                                                                                                                              <  2:1735207/150‑8 (MQ=255)
                         cgcTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTg                                                                                                                             <  1:531067/150‑1 (MQ=255)
                          gcTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCACCGGCGGCGGCCACCTGGCCGCGGTCGGTgg                                                                                                                            <  2:1410501/150‑1 (MQ=255)
                             gtgGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCa                                                                                                                         <  2:1468111/150‑1 (MQ=255)
                              tgGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCAt                                                                                                                        <  2:1813436/150‑1 (MQ=255)
                                ggTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGc                                                                                                                      <  2:535122/150‑1 (MQ=255)
                                   cgcgCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGa                                                                                                                   <  1:1069832/150‑1 (MQ=255)
                                     cgcCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATc                                                                                                                 >  2:490332/1‑150 (MQ=255)
                                      gcCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATc                                                                                                                 >  2:2073863/1‑149 (MQ=255)
                                      gcCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATc                                                                                                                 <  1:2073863/149‑1 (MQ=255)
                                       ccAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCaa                                                                                                               <  1:1442710/150‑1 (MQ=255)
                                        cAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAac                                                                                                              >  2:2265817/1‑150 (MQ=255)
                                          gTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAacac                                                                                                            >  2:1784900/1‑150 (MQ=255)
                                             gCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACcgc                                                                                                         >  1:457057/1‑150 (MQ=255)
                                              cTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACGGATGCTCCCAGCTGACGCGGCGCGAA‑GGTCGGATTACCTTGATCGACATGTCTACGCCCCAGGAATCGCCGGCCGCCACCTGGCCGCGGTCGGTGGCCGTGCCCATCACCACggcg                                                                                                        >  2:1148037/1‑150 (MQ=255)
                                                     aggcTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcggt                                                                                                                              >  2:2189734/1‑121 (MQ=255)
                                                     aggcTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcggt                                                                                                                              <  1:2189734/121‑1 (MQ=255)
                                                     aggcTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGcc                                                                                                 <  1:467473/150‑1 (MQ=255)
                                                             aCGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcg                                                                                         >  1:1210197/1‑150 (MQ=255)
                                                             aCGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcg                                                                                         >  2:1893050/1‑150 (MQ=255)
                                                              cGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGGA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcgc                                                                                        >  2:1046545/1‑150 (MQ=255)
                                                                 cTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGTGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGc                                                                                     <  2:466596/150‑1 (MQ=255)
                                                                  tGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCg                                                                                    <  1:1893050/150‑1 (MQ=255)
                                                                   gCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAACCCTCGGA‑TTACATGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCgg                                                                                   >  1:2254613/1‑150 (MQ=255)
                                                                   gCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCACCACCGCGTCGTGCCCGAAAGCGCTGCgg                                                                                   <  1:2156282/150‑1 (MQ=255)
                                                                   gCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGAGAC‑GGTCGGTTTTGTCTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCgg                                                                                   >  1:2254669/1‑150 (MQ=255)
                                                                     gAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTg                                                                                                                             >  2:1070622/1‑106 (MQ=255)
                                                                     gAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTg                                                                                                                             <  1:1070622/106‑1 (MQ=255)
                                                                        gCGGAACTGATGCTCCCAGCTGTCGCTGCTCAAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAg                                                                              >  1:2275704/1‑150 (MQ=255)
                                                                             aCTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGc                                                                         <  2:1440227/150‑1 (MQ=255)
                                                                             aCTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTGGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGc                                                                         <  2:993760/150‑1 (MQ=255)
                                                                               tGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCg                                                                       >  2:2300990/1‑150 (MQ=255)
                                                                                 aTGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGAt                                                                     >  2:1646811/1‑150 (MQ=255)
                                                                                  tGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATATCGCGTTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATg                                                                    <  2:2275704/150‑1 (MQ=255)
                                                                                    cTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGtt                                                                  >  2:1775045/1‑150 (MQ=255)
                                                                                    cTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGtt                                                                  <  1:2300990/150‑1 (MQ=255)
                                                                                       ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcgc                                                                                        <  1:423621/125‑1 (MQ=255)
                                                                                       ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcgc                                                                                        >  2:423621/1‑125 (MQ=255)
                                                                                       ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGc                                                                             <  1:1189049/136‑1 (MQ=255)
                                                                                       ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGaa                                                               <  2:289744/150‑1 (MQ=255)
                                                                                       ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGCGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGc                                                                             >  2:1189049/1‑136 (MQ=255)
                                                                                          gCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTg                                                            <  1:490332/150‑1 (MQ=255)
                                                                                          gCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTg                                                            <  2:1590884/150‑1 (MQ=255)
                                                                                           cTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTgg                                                           <  1:1046545/150‑1 (MQ=255)
                                                                                                gCTGCGCGAA‑GGTCGGATATCCTTGATGTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCttt                                                      >  1:1147178/1‑150 (MQ=255)
                                                                                                 cTGCGCGAA‑GGTCGGATTTCCATGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCAGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAGGCTGGCCGATGTTGAACTGGCCTTTc                                                     <  2:1210197/150‑1 (MQ=255)
                                                                                                     gcgAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGc                                                 <  1:1784900/150‑1 (MQ=255)
                                                                                                         a‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCAt                                             >  1:1810489/1‑150 (MQ=255)
                                                                                                            gTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGc                                                                                     >  1:1302235/1‑108 (MQ=255)
                                                                                                            gTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGc                                                                                     <  2:1302235/108‑1 (MQ=255)
                                                                                                             tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGtt                                                                  <  1:2197124/126‑1 (MQ=255)
                                                                                                             tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGtt                                                                  >  2:2197124/1‑126 (MQ=255)
                                                                                                             tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTg                                                            >  2:1386351/1‑132 (MQ=255)
                                                                                                             tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTg                                                            <  1:1386351/132‑1 (MQ=255)
                                                                                                             tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCACGAGTCTCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTc                                          >  1:885531/1‑150 (MQ=255)
                                                                                                              cGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGACGATGTTGAACTGGCCTTTCCAGCCCATTTcc                                         <  2:1341412/150‑1 (MQ=255)
                                                                                                                  tttCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAACGCGCTGCCGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTgg                                     >  1:146372/1‑150 (MQ=255)
                                                                                                                      cTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGc                                 >  1:1736627/1‑150 (MQ=255)
                                                                                                                      cTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGc                                 >  1:1220679/1‑150 (MQ=255)
                                                                                                                       ttGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCa                                                   <  2:2009563/131‑1 (MQ=255)
                                                                                                                       ttGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCa                                                   >  1:2009563/1‑131 (MQ=255)
                                                                                                                       ttGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCAGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCg                                >  1:1634880/1‑150 (MQ=255)
                                                                                                                            cTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCAtt                           >  2:640149/1‑150 (MQ=255)
                                                                                                                               cATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGcc                        <  1:1274152/150‑1 (MQ=255)
                                                                                                                               cATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCGGCGGCATTGcc                        <  1:2265817/150‑1 (MQ=255)
                                                                                                                                     gCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATg                  <  1:1756849/150‑1 (MQ=255)
                                                                                                                                     gCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATg                  <  1:2100233/150‑1 (MQ=255)
                                                                                                                                         tCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAg              >  2:1557317/1‑150 (MQ=255)
                                                                                                                                          cccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGt             >  2:2397945/1‑150 (MQ=255)
                                                                                                                                           ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTgg                                                                          >  1:910762/1‑88 (MQ=255)
                                                                                                                                           ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTgg                                                                          <  2:910762/88‑1 (MQ=255)
                                                                                                                                           ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGc                                                                         >  1:1830491/1‑89 (MQ=255)
                                                                                                                                           ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGc                                                                         <  2:1830491/89‑1 (MQ=255)
                                                                                                                                           ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGNGtt            <  2:1508419/150‑1 (MQ=255)
                                                                                                                                           ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGtt            <  2:582848/150‑1 (MQ=255)
                                                                                                                                               tCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGa        <  1:1775045/150‑1 (MQ=255)
                                                                                                                                                 aTCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGAGc      >  1:849623/1‑150 (MQ=255)
                                                                                                                                                  tCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCg                                <  1:130515/123‑1 (MQ=255)
                                                                                                                                                  tCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCg                                >  2:130515/1‑123 (MQ=255)
                                                                                                                                                     gcggcggcCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGAGCCCAg  >  2:42757/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGACAACACATGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGAGCCCAG  >  NC_002947/3667560‑3667857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑