Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,667,718 C→G 6.4% V338L (GTG→CTG)  PP_3231 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,667,7180CG 6.4% 298.4 / 8.2 94V338L (GTG→CTG) PP_3231hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/45);  new base G (2/4);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

ATGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGAGCCCAGACCACCGCC  >  NC_002947/3667569‑3667866
                                                                                                                                                      |                                                                                                                                                    
aTGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCAc                                                                                                                                                      >  2:2449280/1‑150 (MQ=255)
 tGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCAcc                                                                                                                                                     >  1:1813436/1‑150 (MQ=255)
   gcgcAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTg                                                                                                                                                   >  1:669979/1‑150 (MQ=255)
   gcgcAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTg                                                                                                                                                   <  1:1089946/150‑1 (MQ=255)
    cgcAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTgg                                                                                                                                                  <  1:445904/150‑1 (MQ=255)
          tCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGTGGCCACCTGGCCGcgg                                                                                                                                            >  1:466596/1‑150 (MQ=255)
           ccTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGCCACCGACCGCGGCCAGGTGGCcgccgc                                                                                                                                           <  1:1929940/150‑4 (MQ=255)
           ccTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGCCACCGACCGCGGCCAGGTGGCcgccgc                                                                                                                                           <  1:1928595/150‑4 (MQ=255)
            cTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCACCGACCGCGGCCAGGTGGCcgccgcc                                                                                                                                          >  1:1735207/1‑146 (MQ=255)
             ttGCGCTGTTGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcg                                                                                                                                         <  1:100683/150‑1 (MQ=255)
             ttGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGTCTGGCACGCCTGCGAGGCGGAACTGATCCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcg                                                                                                                                         <  1:1106447/150‑1 (MQ=255)
             ttGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGTCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACGTGGCCGcggtcg                                                                                                                                         >  2:1330789/1‑150 (MQ=255)
             ttGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcg                                                                                                                                         >  2:1756849/1‑150 (MQ=255)
               gcgcTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACTCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCACCGACCGCGGCCAGGTGGCcgccgccgat                                                                                                                                       <  1:1196960/150‑8 (MQ=255)
               gcgcTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCACCGACCGCGGCCAGGTGGCcgccgccgat                                                                                                                                       <  2:1735207/150‑8 (MQ=255)
                cgcTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTg                                                                                                                                      <  1:531067/150‑1 (MQ=255)
                 gcTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCACCGGCGGCGGCCACCTGGCCGCGGTCGGTgg                                                                                                                                     <  2:1410501/150‑1 (MQ=255)
                    gtgGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCa                                                                                                                                  <  2:1468111/150‑1 (MQ=255)
                     tgGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCAt                                                                                                                                 <  2:1813436/150‑1 (MQ=255)
                       ggTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGc                                                                                                                               <  2:535122/150‑1 (MQ=255)
                          cgcgCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGa                                                                                                                            <  1:1069832/150‑1 (MQ=255)
                            cgcCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATc                                                                                                                          >  2:490332/1‑150 (MQ=255)
                             gcCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATc                                                                                                                          <  1:2073863/149‑1 (MQ=255)
                             gcCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATc                                                                                                                          >  2:2073863/1‑149 (MQ=255)
                              ccAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCaa                                                                                                                        <  1:1442710/150‑1 (MQ=255)
                               cAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAac                                                                                                                       >  2:2265817/1‑150 (MQ=255)
                                 gTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAacac                                                                                                                     >  2:1784900/1‑150 (MQ=255)
                                    gCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACcgc                                                                                                                  >  1:457057/1‑150 (MQ=255)
                                     cTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACGGATGCTCCCAGCTGACGCGGCGCGAA‑GGTCGGATTACCTTGATCGACATGTCTACGCCCCAGGAATCGCCGGCCGCCACCTGGCCGCGGTCGGTGGCCGTGCCCATCACCACggcg                                                                                                                 >  2:1148037/1‑150 (MQ=255)
                                            aggcTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcggt                                                                                                                                       >  2:2189734/1‑121 (MQ=255)
                                            aggcTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGcggtcggt                                                                                                                                       <  1:2189734/121‑1 (MQ=255)
                                            aggcTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGcc                                                                                                          <  1:467473/150‑1 (MQ=255)
                                                    aCGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcg                                                                                                  >  1:1210197/1‑150 (MQ=255)
                                                    aCGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcg                                                                                                  >  2:1893050/1‑150 (MQ=255)
                                                     cGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGGA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcgc                                                                                                 >  2:1046545/1‑150 (MQ=255)
                                                        cTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGTGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGc                                                                                              <  2:466596/150‑1 (MQ=255)
                                                         tGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCg                                                                                             <  1:1893050/150‑1 (MQ=255)
                                                          gCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAACCCTCGGA‑TTACATGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCgg                                                                                            >  1:2254613/1‑150 (MQ=255)
                                                          gCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCACCACCGCGTCGTGCCCGAAAGCGCTGCgg                                                                                            <  1:2156282/150‑1 (MQ=255)
                                                          gCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGAGAC‑GGTCGGTTTTGTCTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCgg                                                                                            >  1:2254669/1‑150 (MQ=255)
                                                            gAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTg                                                                                                                                      <  1:1070622/106‑1 (MQ=255)
                                                            gAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTg                                                                                                                                      >  2:1070622/1‑106 (MQ=255)
                                                               gCGGAACTGATGCTCCCAGCTGTCGCTGCTCAAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAg                                                                                       >  1:2275704/1‑150 (MQ=255)
                                                                    aCTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGc                                                                                  <  2:1440227/150‑1 (MQ=255)
                                                                    aCTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTGGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGc                                                                                  <  2:993760/150‑1 (MQ=255)
                                                                      tGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCg                                                                                >  2:2300990/1‑150 (MQ=255)
                                                                        aTGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGAt                                                                              >  2:1646811/1‑150 (MQ=255)
                                                                         tGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATATCGCGTTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATg                                                                             <  2:2275704/150‑1 (MQ=255)
                                                                           cTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGtt                                                                           <  1:2300990/150‑1 (MQ=255)
                                                                           cTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGtt                                                                           >  2:1775045/1‑150 (MQ=255)
                                                                              ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcgc                                                                                                 >  2:423621/1‑125 (MQ=255)
                                                                              ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAgcgc                                                                                                 <  1:423621/125‑1 (MQ=255)
                                                                              ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGc                                                                                      <  1:1189049/136‑1 (MQ=255)
                                                                              ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGaa                                                                        <  2:289744/150‑1 (MQ=255)
                                                                              ccAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGCGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGc                                                                                      >  2:1189049/1‑136 (MQ=255)
                                                                                 gCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTg                                                                     <  2:1590884/150‑1 (MQ=255)
                                                                                 gCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTg                                                                     <  1:490332/150‑1 (MQ=255)
                                                                                  cTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTgg                                                                    <  1:1046545/150‑1 (MQ=255)
                                                                                       gCTGCGCGAA‑GGTCGGATATCCTTGATGTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCttt                                                               >  1:1147178/1‑150 (MQ=255)
                                                                                        cTGCGCGAA‑GGTCGGATTTCCATGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCAGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAGGCTGGCCGATGTTGAACTGGCCTTTc                                                              <  2:1210197/150‑1 (MQ=255)
                                                                                            gcgAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGc                                                          <  1:1784900/150‑1 (MQ=255)
                                                                                                a‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCAt                                                      >  1:1810489/1‑150 (MQ=255)
                                                                                                   gTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGc                                                                                              >  1:1302235/1‑108 (MQ=255)
                                                                                                   gTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGc                                                                                              <  2:1302235/108‑1 (MQ=255)
                                                                                                    tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGtt                                                                           >  2:2197124/1‑126 (MQ=255)
                                                                                                    tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGtt                                                                           <  1:2197124/126‑1 (MQ=255)
                                                                                                    tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTg                                                                     >  2:1386351/1‑132 (MQ=255)
                                                                                                    tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTg                                                                     <  1:1386351/132‑1 (MQ=255)
                                                                                                    tCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCACGAGTCTCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTc                                                   >  1:885531/1‑150 (MQ=255)
                                                                                                     cGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGACGATGTTGAACTGGCCTTTCCAGCCCATTTcc                                                  <  2:1341412/150‑1 (MQ=255)
                                                                                                         tttCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAACGCGCTGCCGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTgg                                              >  1:146372/1‑150 (MQ=255)
                                                                                                             cTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGc                                          >  1:1220679/1‑150 (MQ=255)
                                                                                                             cTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGc                                          >  1:1736627/1‑150 (MQ=255)
                                                                                                              ttGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCa                                                            <  2:2009563/131‑1 (MQ=255)
                                                                                                              ttGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCa                                                            >  1:2009563/1‑131 (MQ=255)
                                                                                                              ttGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCAGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCg                                         >  1:1634880/1‑150 (MQ=255)
                                                                                                                   cTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCAtt                                    >  2:640149/1‑150 (MQ=255)
                                                                                                                      cATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGcc                                 <  1:1274152/150‑1 (MQ=255)
                                                                                                                      cATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCGGCGGCATTGcc                                 <  1:2265817/150‑1 (MQ=255)
                                                                                                                            gCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATg                           <  1:2100233/150‑1 (MQ=255)
                                                                                                                            gCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATg                           <  1:1756849/150‑1 (MQ=255)
                                                                                                                                tCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAg                       >  2:1557317/1‑150 (MQ=255)
                                                                                                                                 cccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGt                      >  2:2397945/1‑150 (MQ=255)
                                                                                                                                  ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTgg                                                                                   <  2:910762/88‑1 (MQ=255)
                                                                                                                                  ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTgg                                                                                   >  1:910762/1‑88 (MQ=255)
                                                                                                                                  ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGc                                                                                  <  2:1830491/89‑1 (MQ=255)
                                                                                                                                  ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGc                                                                                  >  1:1830491/1‑89 (MQ=255)
                                                                                                                                  ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGNGtt                     <  2:1508419/150‑1 (MQ=255)
                                                                                                                                  ccAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGtt                     <  2:582848/150‑1 (MQ=255)
                                                                                                                                      tCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGa                 <  1:1775045/150‑1 (MQ=255)
                                                                                                                                        aTCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGAGc               >  1:849623/1‑150 (MQ=255)
                                                                                                                                         tCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCg                                         <  1:130515/123‑1 (MQ=255)
                                                                                                                                         tCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCg                                         >  2:130515/1‑123 (MQ=255)
                                                                                                                                            gcggcggcCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGAGCCCAg           >  2:42757/1‑150 (MQ=255)
                                                                                                                                            accgcggcCAGGTGGCCGCGGGCGGTGGCCATGCCGATAAACCCGGCGTTTTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGCTGTGAGCCCAg           >  1:553087/4‑150 (MQ=255)
                                                                                                                                                     aCCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGAGCCCAGACCACCGcc  >  1:2143073/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
ATGGCGCAGCTCCTTGCGCTGTGGGTCGCGCCAGTCGCTCAGGCAGGCTGGCACGCCTGCGAGGCGGAACTGATGCTCCCAGCTGTCGCTGCGCGAA‑GGTCGGATTTCCTTGATCTGCATGTCGCCGTCCCAGTCATCGGCGGCGGCCACCTGGCCGCGGTCGGTGGCCATGCCGATCAACACCGCGTCGTGCCCGAAAGCGCTGCGGCAAAGCTGGCCGATGTTGAACTGGCCTTTCCAGCCCATTTCCGTGGCTGCGGCATTGCCGATATGGGAGTTGTGAGCCCAGACCACCGCC  >  NC_002947/3667569‑3667866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑