Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,280,092	C→G	6.4%	P416P (CCC→CCG)	PP_3750 →	GntR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,280,092	0	C	G	6.4%	296.3 / 9.8	94	P416P (CCC→CCG)	PP_3750	GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (36/52);  new base G (3/3);  total (39/55)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

ACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGATATCGAC  >  NC_002947/4279947‑4280233                                                                                                                                                    |                                                                                                                                                 aCCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCGgggg                                                                                                                                              >  2:1047882/1‑150 (MQ=255) aCCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCgggg                                                                                                                                              <  1:1339829/150‑1 (MQ=255)  ccTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGt                                                                                                                                             <  1:2447885/150‑1 (MQ=255)   cTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCGGGGGTg                                                                                                                                            <  1:1047882/150‑1 (MQ=255)    tGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTgg                                                                                                                                           <  2:1476290/150‑1 (MQ=255)     gAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGa                                                                                                                                          <  1:2360170/150‑1 (MQ=255)      aaCAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAt                                                                                                                                         <  2:192922/150‑1 (MQ=255)        cAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGTCGGCCATCTGGCACCGTGC‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCCCCTCAGTGCCTTGGCCCCCCCCGGGGTGGAtgt                                                                                                                                       <  2:1149740/150‑1 (MQ=255)        cAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCGGGGGTggccaa                                                                                                                                       <  1:827784/150‑5 (MQ=255)        cAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgt                                                                                                                                       <  2:477585/150‑1 (MQ=255)        cAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgt                                                                                                                                       <  2:1830292/150‑1 (MQ=255)        cAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgt                                                                                                                                       <  2:898024/150‑1 (MQ=255)         aaGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCTCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgtg                                                                                                                                      <  2:1023788/150‑1 (MQ=255)         aaGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgtg                                                                                                                                      >  2:528526/1‑150 (MQ=255)         aaGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgtg                                                                                                                                      >  2:768481/1‑150 (MQ=255)         aaGCACATCCGCCGCTGATATGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCGGGGGTggccaag                                                                                                                                      <  1:1305942/150‑6 (MQ=255)           gCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGa                                                                                                                                    <  2:1309340/150‑1 (MQ=255)             acaTCCGCCGCTGCCACGAGGTCTACAGCGGACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGc                                                                                                                                  >  2:1403586/1‑150 (MQ=255)             acaTCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgt                                                                                                                                       <  2:293777/145‑1 (MQ=255)             acaTCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgt                                                                                                                                       >  1:293777/1‑145 (MQ=255)             acaTCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGc                                                                                                                                  >  1:105116/1‑150 (MQ=255)             acaTCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGc                                                                                                                                  <  1:294992/150‑1 (MQ=255)                  cgccgcTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCCTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTg                                                                                                                             <  2:1289188/150‑1 (MQ=255)                   gtcgcTGTCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCGCCCCCGGGGTGGATGTGGAGCTGTTgg                                                                                                                            >  2:1273559/3‑150 (MQ=255)                   gccgcTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGGTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTgg                                                                                                                            <  1:712687/150‑1 (MQ=255)                   gccgcTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTgg                                                                                                                            <  2:1679248/150‑1 (MQ=255)                   gccgcTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTgg                                                                                                                            <  2:1990363/150‑1 (MQ=255)                   gccgcTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTgg                                                                                                                            <  2:387242/150‑1 (MQ=255)                            aCGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTcg                                                                                                                   >  2:319780/1‑150 (MQ=255)                                ggTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTcgcgcg                                                                                                               <  2:105116/150‑1 (MQ=255)                                   cTACAGCCCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCCCCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTaa                                                                                                            <  2:953700/150‑1 (MQ=255)                                    tACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCTCCATCCCCGCCAGCGCAGGCTTCCACATAAGTGGCATGGGCGCCGTCTGGGGGGAGAGGGAGCGCTTGGCCAACCTCGCGCGTAag                                                                                                           >  2:1331344/1‑150 (MQ=255)                                    tACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAag                                                                                                           >  1:359085/1‑150 (MQ=255)                                       aGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGTACAGTCGTTTTCAGCG‑GAT‑GCTTTGTTCTCAGGCGTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggta                                                                                                        <  1:768481/150‑1 (MQ=255)                                        gcgcACGCCGCTAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtag                                                                                                       <  1:1697659/150‑1 (MQ=255)                                        gcgcACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtag                                                                                                       <  2:1945173/150‑1 (MQ=255)                                         cgcACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtaga                                                                                                      <  2:2294386/150‑1 (MQ=255)                                            aCGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtagaggt                                                                                                   >  1:600549/1‑150 (MQ=255)                                            aCGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTAA‑‑ACGAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtagaggt                                                                                                   <  1:1331344/150‑1 (MQ=255)                                              gccgcGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtagaggt‑‑‑ag                                                                                              >  2:1121812/1‑150 (MQ=255)                                              gccgcGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtagaggt‑‑‑ag                                                                                              <  1:1121812/150‑1 (MQ=255)                                                cgcgAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGc                                                                                            >  2:5624/1‑150 (MQ=255)                                                 gcgAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGcc                                                                                           >  1:686865/1‑150 (MQ=255)                                                  cgAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCACGCGCGTAAGGTAGAGGT‑‑‑AGGCCt                                                                                          <  2:1622961/150‑1 (MQ=255)                                                       gcATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTAtt                                                                                     <  1:779904/150‑1 (MQ=255)                                                         aTCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCa                                                                                   >  2:703713/1‑150 (MQ=255)                                                         aTCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCa                                                                                   >  1:1661331/1‑150 (MQ=255)                                                           ccTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACt                                                                                 <  2:686865/150‑1 (MQ=255)                                                             tGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTgg                                                                               >  2:1895798/1‑150 (MQ=255)                                                               gcgcgcCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCa                                                                             <  2:2129155/150‑1 (MQ=255)                                                                 gcgcCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCAcc                                                                           >  2:611773/1‑150 (MQ=255)                                                                 gcgcCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCAcc                                                                           <  1:5624/150‑1 (MQ=255)                                                                 gcgcCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTACACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCAcc                                                                           >  2:1492805/1‑150 (MQ=255)                                                                  cgcCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCAccc                                                                          <  1:1403586/150‑1 (MQ=255)                                                                   gcCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCct                                                                         >  1:1626813/1‑150 (MQ=255)                                                                         gcggcgATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCa                                                                   <  1:1585245/150‑1 (MQ=255)                                                                         gcggcgATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGAAACCGCGCTCCGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCa                                                                   <  2:1638491/150‑1 (MQ=255)                                                                             cgATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGa                                                               <  1:1882622/150‑1 (MQ=255)                                                                             cgATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAGGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGa                                                               <  1:2143117/150‑1 (MQ=255)                                                                              gATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAg                                                              <  1:1111687/150‑1 (MQ=255)                                                                                  tGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCATCTTCAGCGAGGCAc                                                          >  2:2028988/1‑150 (MQ=255)                                                                                    gCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGC‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCg                                                        >  1:607090/1‑150 (MQ=255)                                                                                      aCCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGt                                                      >  2:2198426/1‑150 (MQ=255)                                                                                        cGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTGCACCTCAGCGCATATCAATCCCCCGGGGTGGATTTCTATCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCGTCTTCAGCGAGGCACCGGTAc                                                    >  1:2254304/1‑150 (MQ=255)                                                                                                tCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCgg                                              <  1:2198426/150‑1 (MQ=255)                                                                                                  aGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGATGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCgggg                                            >  1:2433144/1‑150 (MQ=255)                                                                                                  aGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGTT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGCTACGGCCgggg                                            >  2:269770/1‑150 (MQ=255)                                                                                                  aGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTTTTCAGCGAGGCACCGGTACGGCCgggg                                            <  1:1870924/150‑1 (MQ=255)                                                                                                    cgcCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTg                                                                                                                                >  1:218717/1‑67 (MQ=255)                                                                                                    cgcCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTg                                                                                                                                <  2:218717/67‑1 (MQ=255)                                                                                                     gcCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGGTGTTGCCCAACCGCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTa                                         <  1:542489/150‑1 (MQ=255)                                                                                                       cATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTAtt                                       >  2:515727/1‑150 (MQ=255)                                                                                                        aTCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTg                                      >  2:185214/1‑150 (MQ=255)                                                                                                        aTCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTg                                      >  1:193722/1‑150 (MQ=255)                                                                                                          ccccGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGAt                                    <  1:528526/150‑1 (MQ=255)                                                                                                            ccGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATgg                                  >  2:19746/1‑150 (MQ=255)                                                                                                                       ggCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCgggg                                            <  2:375176/129‑1 (MQ=255)                                                                                                                       ggCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCgggg                                            >  1:375176/1‑129 (MQ=255)                                                                                                                        gCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTGAAAGGCC‑G‑‑TCCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGATAATGCTGGGCTTCGGCGCCa                      >  1:746560/1‑150 (MQ=255)                                                                                                                         cTTCCACCTCAGTTCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAGCCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCAt                     <  2:1297652/150‑1 (MQ=255)                                                                                                                          ttCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATc                    >  2:661254/1‑150 (MQ=255)                                                                                                                             cACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGaa                 <  2:240806/150‑1 (MQ=255)                                                                                                                              aCCTAAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGTCATTGATGGGCTTCGGCGCCAGCGAAc                >  1:1083923/1‑150 (MQ=255)                                                                                                                                 tCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAActgc             <  1:611773/150‑1 (MQ=255)                                                                                                                                   aGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGaa                 >  2:1063423/1‑144 (MQ=255)                                                                                                                                   aGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGaa                 <  1:1063423/144‑1 (MQ=255)                                                                                                                                   aGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAActgctg           <  2:1753046/150‑1 (MQ=255)                                                                                                                                      tccacatccACCCCGGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTAACTGGCACCCTTCTTCAGCGCGGAACCGGGACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGat        >  1:535592/8‑150 (MQ=255)                                                                                                                                       ccgtGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTAt                                        <  2:1688257/114‑1 (MQ=255)                                                                                                                                       ccTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTAt                                        >  1:1688257/1‑117 (MQ=255)                                                                                                                                       ccTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGata       <  1:185214/150‑1 (MQ=255)                                                                                                                                          catccACCCCGGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGATATCg    >  1:1889962/4‑150 (MQ=255)                                                                                                                                           ggCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGATATCGa   <  2:1684233/150‑1 (MQ=255)                                                                                                                                            gCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGATATCGAc  <  1:703713/150‑1 (MQ=255)                                                                                                                                                 ccccGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTAt                                        <  1:996757/107‑1 (MQ=255)                                                                                                                                                 ccccGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTAt                                        >  2:996757/1‑107 (MQ=255)                                                                                                                                                    cGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGc                                <  1:1010834/112‑1 (MQ=255)                                                                                                                                                    cGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGc                                >  2:1010834/1‑112 (MQ=255)                                                                                                                                                    |                                                                                                                                                 ACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGG‑‑TTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGT‑‑‑AGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGATATCGAC  >  NC_002947/4279947‑4280233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑