BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,375,488	A→T	8.3%	V31D (GTC→GAC)	PP_5637 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,375,488	0	A	T	8.3%	365.9 / 16.8	131	V31D (GTC→GAC)	PP_5637	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (66/53); new base T (6/5); total (72/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 136 total aligned reads displayed.

ACCACGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAA  >  NC_002947/4375339‑4375636

accacGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGACGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGa                                                                                                                                                      <  1:1996754/150‑1 (MQ=255)

 ccacGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGTTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGAc                                                                                                                                                     >  1:386373/1‑150 (MQ=255)

   acGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTc                                                                                                                                                   >  2:1687573/1‑150 (MQ=255)

         gcgcATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCa                                                                                                                                             <  2:274148/150‑1 (MQ=255)

         gcgcATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCa                                                                                                                                             <  2:395078/150‑1 (MQ=255)

            cATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCagag                                                                                                                                          <  1:1950686/150‑1 (MQ=255)

            cATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGAGTCGCGACCCaggc                                                                                                                                          <  1:751991/150‑3 (MQ=255)

             atatAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCAAGGCGTAGGGTTAAACACCCAGGCCTAGGTCGAGACTCCACCCagatt                                                                                                                                         <  2:90898/150‑3 (MQ=255)

              tataGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGACTTTCACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGc                                                                                                                                        >  1:655235/1‑150 (MQ=255)

                       ccTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGTCTCGACCCAGAGGCTCGCTGTGa                                                                                                                               <  2:1184011/150‑1 (MQ=255)

                         ttCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATg                                                                                                                             >  2:2144472/1‑150 (MQ=255)

                               ctctTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGcc                                                                                                                       >  1:1599589/1‑150 (MQ=255)

                               ctctTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGcc                                                                                                                       >  2:817475/1‑150 (MQ=255)

                                tcttctACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTTGCAGCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCa                                                                                                                      >  1:1239669/1‑150 (MQ=255)

                                tcttctACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGAACTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCa                                                                                                                      >  1:1021816/1‑150 (MQ=255)

                                tctgctACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCa                                                                                                                      >  2:1521709/1‑150 (MQ=255)

                                 cttctACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCaa                                                                                                                     <  2:2095546/150‑1 (MQ=255)

                                  ttctACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAAt                                                                                                                    >  1:924028/1‑150 (MQ=255)

                                     tACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGAGTCGCGACCCag                                                                                                                                            >  1:137562/1‑123 (MQ=255)

                                     tACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGAGTCGCGACCCag                                                                                                                                            <  2:137562/123‑1 (MQ=255)

                                      aCCCCGTTGATGTTGAGGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTgg                                                                                                                <  2:1783662/150‑1 (MQ=255)

                                       ccccGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGAc                                                                                                                                                     <  1:1383168/112‑1 (MQ=255)

                                        cccGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGAc                                                                                                              >  1:209848/1‑150 (MQ=255)

                                          cGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGc                                                                                                                                        <  1:182591/122‑1 (MQ=255)

                                           gttgatgttgatGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTc                                                                                                           >  1:1828347/1‑150 (MQ=255)

                                            ttgatgttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTcc                                                                                                          <  1:1521709/150‑1 (MQ=255)

                                            ttgatgttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTcc                                                                                                          >  2:69474/1‑150 (MQ=255)

                                              gatgttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCcgc                                                                                                        >  1:236096/1‑150 (MQ=255)

                                                tgttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCcgcgc                                                                                                      <  1:2444207/150‑1 (MQ=255)

                                                tgttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCATAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCcgcgc                                                                                                      >  1:2224433/1‑150 (MQ=255)

                                                  ttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCgg                                                                                                    >  1:2213990/1‑150 (MQ=255)

                                                   tgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGa                                                                                                   <  2:1828347/150‑1 (MQ=255)

                                                   tgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCCACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGa                                                                                                   <  2:2433047/150‑1 (MQ=255)

                                                     atgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAgg                                                                                                 <  2:2213990/150‑1 (MQ=255)

                                                      tgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAggg                                                                                                <  2:2142471/150‑1 (MQ=255)

                                                         cGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGGCGCCAATGTGGACGTCCGCGCGGAGGGGCt                                                                                             <  1:2144472/150‑1 (MQ=255)

                                                         cGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGACGCTCACTTTGATGGTCGCCAAGGTGGACGTCCGCGCGGAGGGGcg                                                                                             >  2:631829/1‑149 (MQ=255)

                                                          gAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTg                                                                                            <  1:2101144/150‑1 (MQ=255)

                                                            gCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGa                                                                                          <  2:1470341/150‑1 (MQ=255)

                                                                  ccATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTcc                                                                                    >  2:477584/1‑150 (MQ=255)

                                                                   cATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTATGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGagatcgga                                                                                   >  1:1262448/1‑143 (MQ=255)

                                                                    aTGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTg                                                                                            <  1:2239194/140‑1 (MQ=255)

                                                                     tGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTa                                                                                 >  2:2050185/1‑150 (MQ=255)

                                                                     tGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTa                                                                                 >  2:1846848/1‑150 (MQ=255)

                                                                      gCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACACGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAg                                                                                <  2:1370145/150‑1 (MQ=255)

                                                                          ttGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCgg                                                                            >  1:1712809/1‑150 (MQ=255)

                                                                            gACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTGGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGcttgg                                                                          <  2:2064907/150‑5 (MQ=255)

                                                                            gACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTc                                                                          <  2:1737676/150‑1 (MQ=255)

                                                                             aCCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGa                                                                                                                                                      <  2:749662/73‑1 (MQ=255)

                                                                              ccTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTg                                                                        >  2:2244779/1‑150 (MQ=255)

                                                                               cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGCTGGTCGCCAATGTGGACATCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTgg                                                                       >  1:34429/1‑149 (MQ=255)

                                                                               cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGa                                                                       >  2:2249487/1‑150 (MQ=255)

                                                                               cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGa                                                                       >  2:1821320/1‑150 (MQ=255)

                                                                               cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGa                                                                       >  1:1620070/1‑150 (MQ=255)

                                                                               cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGa                                                                       >  1:1105266/1‑150 (MQ=255)

                                                                                ttCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAg                                                                      <  1:2249487/150‑1 (MQ=255)

                                                                                ttCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAg                                                                      <  1:1687573/150‑1 (MQ=255)

                                                                                ttCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAg                                                                      <  2:1620070/150‑1 (MQ=255)

                                                                                 tCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAAt                                                                                                                    >  1:451310/1‑103 (MQ=255)

                                                                                 tCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAAt                                                                                                                    <  2:451310/103‑1 (MQ=255)

                                                                                 tCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAgg                                                                     <  1:1857372/150‑1 (MQ=255)

                                                                                   gCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGc                                                                   <  1:2050185/150‑1 (MQ=255)

                                                                                      tCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAg                                                                <  2:924028/150‑1 (MQ=255)

                                                                                       cGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAgg                                                               <  2:236096/150‑1 (MQ=255)

                                                                                         tGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGAc                                                                                                                                                     >  1:1780807/1‑62 (MQ=255)

                                                                                         tGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGcc                                                             >  2:2088039/1‑150 (MQ=255)

                                                                                                gggggATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACGCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGGCTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCAt                                                      >  2:3662/1‑150 (MQ=255)

                                                                                                gggggATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTGGGCGGTCTGAGGGCAAGGCCACGCCAt                                                      <  1:680495/150‑1 (MQ=255)

                                                                                                      tAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTcgc                                                >  1:803537/1‑150 (MQ=255)

                                                                                                      tAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTcgc                                                <  1:1846848/150‑1 (MQ=255)

                                                                                                          gTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCgcggcg                                            >  2:833544/1‑150 (MQ=255)

                                                                                                          gTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCgcggcg                                            >  1:1938299/1‑150 (MQ=255)

                                                                                                           tCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGc                                                                              <  2:1848999/115‑1 (MQ=255)

                                                                                                           tCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGCCGCCAATGTGGACGTCCCCGGGGAGGGCCTGGACTTCCCGTAGGCGGTCTGAGGGCAAGGCCACGACATGCTCGCGGCGt                                           <  2:386373/150‑1 (MQ=255)

                                                                                                               gCCCAGGCGTAGGGTTAAACACACCGCAGGTGGGCGCGGATCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGaa                                       <  1:5/150‑1 (MQ=255)

                                                                                                                 ccAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACg                                     >  2:503537/1‑150 (MQ=255)

                                                                                                                 ccAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACg                                     >  1:1132119/1‑150 (MQ=255)

                                                                                                                  cAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGGCTGAGGGCAAAGCAATGCCATGCTCGCGGCGTCGAACga                                    >  1:602/1‑150 (MQ=255)

                                                                                                                   aGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACgag                                   >  2:1790703/1‑150 (MQ=255)

                                                                                                                    ggCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACgaga                                  <  2:1021816/150‑1 (MQ=255)

                                                                                                                          gggTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGa                                                                                                                               <  2:2230236/51‑1 (MQ=255)

                                                                                                                          gggTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGa                                                                                                                               >  1:2230236/1‑51 (MQ=255)

                                                                                                                                aaCACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCt                                                                                             >  1:1742696/1‑79 (MQ=255)

                                                                                                                                aaCACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCt                                                                                             <  2:1742696/79‑1 (MQ=255)

                                                                                                                                aaCACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATg                      >  2:689093/1‑150 (MQ=255)

                                                                                                                                 acacCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGc                     <  1:477584/150‑1 (MQ=255)

                                                                                                                                   acCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTg                                                                                            >  1:220638/1‑77 (MQ=255)

                                                                                                                                   acCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTg                                                                                            <  2:222009/77‑1 (MQ=255)

                                                                                                                                   acCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCgg                   >  1:1270146/1‑150 (MQ=255)

                                                                                                                                   acCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCgg                   >  2:1680822/1‑150 (MQ=255)

                                                                                                                                        cctcTGGGTCGAGTCGCGACCCAGGGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCa              >  1:915982/4‑150 (MQ=255)

                                                                                                                                         ctcTGGGTCGAGTCGCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCaa             >  1:1560051/3‑150 (MQ=255)

                                                                                                                                          tcTGGGTCGAGTCGCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAAt            >  1:598361/2‑150 (MQ=255)

                                                                                                                                          tcTGGGTCGAGTCGCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAAt            >  1:658929/2‑150 (MQ=255)

                                                                                                                                           cTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCgg                   >  1:2160591/1‑142 (MQ=255)

                                                                                                                                           cTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCgg                   <  2:2160591/142‑1 (MQ=255)

                                                                                                                                            tGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATAc          <  2:1529817/150‑1 (MQ=255)

                                                                                                                                              ggTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTg        <  1:1680822/150‑1 (MQ=255)

                                                                                                                                                  gcgACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGtata    >  1:2226909/1‑150 (MQ=255)

                                                                                                                                                     aCTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTg        <  2:1972471/143‑1 (MQ=255)

ACCACGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAA  >  NC_002947/4375339‑4375636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`