Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,599,336 G→C 6.8% L173L (CTC→CTG PP_4071 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,599,3360GC 6.8% 372.4 / 14.2 118L173L (CTC→CTGPP_4071membrane protein
Reads supporting (aligned to +/- strand):  ref base G (57/53);  new base C (5/3);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 118 total aligned reads displayed.

GCTTGGGTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGTACAGCGCGC  >  NC_002947/4599193‑4599478
                                                                                                                                               |                                                                                                                                              
gCTTGGGTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGcc                                                                                                                                          >  2:2214298/1‑150 (MQ=255)
 cTTGGGTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCt                                                                                                                                         >  2:2325835/1‑150 (MQ=255)
     ggTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTgcgc                                                                                                                                     >  2:1642883/1‑150 (MQ=255)
     ggTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTgcgc                                                                                                                                     <  1:2361651/150‑1 (MQ=255)
      gTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCAGGGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCt                                                                                                                                    <  1:416413/150‑1 (MQ=255)
       ttCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTg                                                                                                                                   >  2:916404/1‑150 (MQ=255)
          cAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTggcc                                                                                                                                <  2:425464/150‑1 (MQ=255)
                gTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGcccc                                                                                                                          <  1:2108557‑M2/150‑5 (MQ=255)
                 tACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGccccg                                                                                                                         <  2:423024‑M2/150‑6 (MQ=255)
                  aCCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGccccgt                                                                                                                        >  1:486939‑M2/1‑144 (MQ=255)
                     gAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGCCGGCCAGCGCAGGCGCTCGCCTAccccgtggg                                                                                                                     >  1:1435954‑M2/1‑141 (MQ=255)
                          cTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTgcgc                                                                                                                <  1:2214298/150‑1 (MQ=255)
                           tCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGcc                                                                                                               >  1:2096000/1‑150 (MQ=255)
                           tCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGcc                                                                                                               >  2:379182/1‑150 (MQ=255)
                           tCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGcc                                                                                                               >  1:267444/1‑150 (MQ=255)
                           tCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGcc                                                                                                               >  2:1493075/1‑150 (MQ=255)
                           tCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGcc                                                                                                               >  2:126132/1‑150 (MQ=255)
                             aaCGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAgc                                                                                                                                             >  1:1780590/1‑118 (MQ=255)
                             aaCGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAgc                                                                                                                                             <  2:1780590/118‑1 (MQ=255)
                             aaCGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCtt                                                                                                             <  1:107759/150‑1 (MQ=255)
                                gTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTc                                                                                                          >  2:2241515/1‑150 (MQ=255)
                                   aGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGAt                                                                                                       >  1:1929404/1‑150 (MQ=255)
                                   aGTGGAGTCACAGCTCAGAGAATTTACGGGGACGATCAGACTGTGAAAGGCACCGGGATGGGCGATGATCAGCGCATCATTGCTGATCTACCACTCCACGTGGTAGGCGCGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGAt                                                                                                       >  2:628795/1‑150 (MQ=255)
                                    gTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGCCGGCCAGCGCAGGCGCTCGCCTAccccgtggggtggtggatcagcaa                                                                                                      >  2:2219249‑M2/1‑126 (MQ=255)
                                     tGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTTCCTGCGCCTTGTCGATGc                                                                                                     <  1:2267753/150‑1 (MQ=255)
                                       gAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCgg                                                                                                   <  1:1493075/150‑1 (MQ=255)
                                        aGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATCGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCggg                                                                                                  <  1:885145/150‑1 (MQ=255)
                                          tCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTc                                                                                                <  2:1046006/150‑1 (MQ=255)
                                          tCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTc                                                                                                >  1:1046006/1‑150 (MQ=255)
                                            gcTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCaa                                                                                              <  2:503383/149‑1 (MQ=255)
                                            aCTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGTCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCaa                                                                                              >  1:301089/1‑150 (MQ=255)
                                             cTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCgg                                                                                                   <  1:1903366/144‑1 (MQ=255)
                                             cTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCgg                                                                                                   >  2:1903366/1‑144 (MQ=255)
                                               gCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACga                                                                                           >  2:872516/1‑150 (MQ=255)
                                                cccAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGcc                                                                                                                     <  2:291400/123‑1 (MQ=255)
                                                cccAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGcc                                                                                                                     >  1:291400/1‑123 (MQ=255)
                                                cccAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGACCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGcc                                                                                                                     <  2:2096178/123‑1 (MQ=255)
                                                cccAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCAGTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGcc                                                                                                                     >  1:2096178/1‑123 (MQ=255)
                                                  cAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGaa                                                                                        >  2:348279/1‑150 (MQ=255)
                                                   aGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGaaa                                                                                       >  1:475371/1‑150 (MQ=255)
                                                   aGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGaaa                                                                                       >  2:203141/1‑150 (MQ=255)
                                                   aGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGaaa                                                                                       >  2:562308/1‑150 (MQ=255)
                                                   aGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCCAGCGCAGGCGCTCGCCTAccccgtgg                                                                                                                      >  1:86921‑M2/1‑111 (MQ=255)
                                                   aGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCCAGCGCAGGCGCTCGCCTAccccgtgg                                                                                                                      <  2:86921‑M2/119‑9 (MQ=255)
                                                       aaTCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCTCCCCGTCGATGCGGGTCAACGAGAAACttt                                                                                   <  1:1880536/150‑1 (MQ=255)
                                                                ggCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGc                                                                          >  1:1415702/1‑150 (MQ=255)
                                                                  ccGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAg                                                                        <  1:203141/150‑1 (MQ=255)
                                                                   cGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGt                                                                       <  2:2108557/150‑1 (MQ=255)
                                                                   cGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGt                                                                       <  2:2096000/150‑1 (MQ=255)
                                                                        tGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGccccgtggggtggtggatcagcaatgatgc                                                                                                <  2:306997‑M2/120‑31 (MQ=255)
                                                                        tGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGccccgtggggtggtggatcagcaatgatgc                                                                                                >  1:306997‑M2/1‑90 (MQ=255)
                                                                         ggCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATaa                                                                 >  2:1129138/1‑150 (MQ=255)
                                                                          gCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGa                                                                                                                                               >  2:1805474/1‑71 (MQ=255)
                                                                          gCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGa                                                                                                                                               <  1:1805474/71‑1 (MQ=255)
                                                                          gCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATaaa                                                                >  1:859598/1‑150 (MQ=255)
                                                                             gATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAgct                                                             >  2:1102091/1‑150 (MQ=255)
                                                                             gATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAgct                                                             <  2:1929404/150‑1 (MQ=255)
                                                                               tAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAgctgc                                                           <  1:2004403/150‑1 (MQ=255)
                                                                                 aaGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGctc                                                         >  1:2376885/1‑150 (MQ=255)
                                                                                    gCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGc                                                                                                                             >  2:700666‑M2/1‑78 (MQ=255)
                                                                                    gCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGc                                                                                                                             <  1:700666‑M2/79‑2 (MQ=255)
                                                                                     cACCGGCCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGAGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGc                                                     <  1:628795/150‑1 (MQ=255)
                                                                                      aCCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGcc                                                    <  1:1990991/150‑1 (MQ=255)
                                                                                         gggcgggTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGgc                                                 <  1:2325835/150‑1 (MQ=255)
                                                                                           gcgggTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGgcct                                               <  1:355473/150‑1 (MQ=255)
                                                                                                  gatgatCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGgc                                        <  1:1827326/150‑1 (MQ=255)
                                                                                                   atgatCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTCGCCTAccccgtggggtggt                                                                                                                <  1:1293019‑M2/77‑15 (MQ=255)
                                                                                                   atgatCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTCGCCTAccccgtggggtggt                                                                                                                >  2:1293019‑M2/1‑63 (MQ=255)
                                                                                                     gatCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGgcctgcct                                           <  1:2168767/144‑1 (MQ=255)
                                                                                                     gatCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGcc                                     <  1:872516/150‑1 (MQ=255)
                                                                                                      atCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGccc                                    >  2:1238083/1‑150 (MQ=255)
                                                                                                       tCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCa                                   <  2:2376885/150‑1 (MQ=255)
                                                                                                       tCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCa                                   >  1:485790/1‑150 (MQ=255)
                                                                                                         aCCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGc                                 >  1:1765217/1‑150 (MQ=255)
                                                                                                          ccGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGTCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCa                                >  1:653373/1‑150 (MQ=255)
                                                                                                          ccGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCa                                <  1:1102091/150‑1 (MQ=255)
                                                                                                            gCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATc                                                                               <  1:489111/101‑1 (MQ=255)
                                                                                                             catcatTGCTGTTCCACCACCCCACGGGGTGGTCGGGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGa                             <  2:301089/150‑1 (MQ=255)
                                                                                                             catcatTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTTATCGGTGCAGTCAATAAAGCTGCTCTTGCCTGCCTGCCTGGCGCCCAGCACGa                             <  1:423024/150‑1 (MQ=255)
                                                                                                             catcatTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGa                             <  1:916404/150‑1 (MQ=255)
                                                                                                             catcatTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGa                             <  2:267444/150‑1 (MQ=255)
                                                                                                             catcatTGCTGATCCACCACCCCACGGGGTAGGAGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGa                             >  1:1379104/1‑150 (MQ=255)
                                                                                                              atcatTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCgg                                                                                                   >  2:564633/1‑79 (MQ=255)
                                                                                                                 atTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGt                                                                                                 >  2:917027/1‑78 (MQ=255)
                                                                                                                 atTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGt                                                                                                 <  1:917027/78‑1 (MQ=255)
                                                                                                                  ttGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTAc                        >  1:2314229/1‑150 (MQ=255)
                                                                                                                     cTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCTACTAGAAACTTTGATCCGTGCACTCACTAATTCATCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCAc                     >  1:2377561/1‑150 (MQ=255)
                                                                                                                      tGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACg                    <  2:485790/150‑1 (MQ=255)
                                                                                                                      tGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACg                    >  2:1630442/1‑150 (MQ=255)
                                                                                                                      tGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACg                    <  1:1129138/150‑1 (MQ=255)
                                                                                                                         tcaaccaccCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCa                 >  1:1816648/4‑150 (MQ=255)
                                                                                                                         tCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAAc                                                                                      <  1:748704/81‑1 (MQ=255)
                                                                                                                         tCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAAc                                                                                      >  2:748704/1‑81 (MQ=255)
                                                                                                                           caccacCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAAt               <  1:126132/150‑1 (MQ=255)
                                                                                                                               accCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGt           >  1:1502988/1‑150 (MQ=255)
                                                                                                                                ccccACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGTa          <  1:1630442/150‑1 (MQ=255)
                                                                                                                                ccccACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGAGCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGTa          >  2:482979/1‑150 (MQ=255)
                                                                                                                                 cccACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGTAc         >  2:262209/1‑150 (MQ=255)
                                                                                                                                  ccACGGGGTAGGCGAGCGCCTGCGCTGGCCGGccccgtggggtggtggatcagcaatgatgcggtgatcatcgacccgcccggtgcctttatca                                                                <  1:650812‑M2/94‑63 (MQ=255)
                                                                                                                                  ccACGGGGTAGGCGAGCGCCTGCGCTGGCCGGccccgtggggtggtggatcagcaatgatgcggtgatcatcgacccgcccggtgcctttatca                                                                >  2:650812‑M2/1‑32 (MQ=255)
                                                                                                                                               |                                                                                                                                              
GCTTGGGTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGTACAGCGCGC  >  NC_002947/4599193‑4599478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: