BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,906,511	A→T	6.0%	L111Q (CTG→CAG)	PP_4315 ←	epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,906,511	0	A	T	6.0%	352.3 / 8.3	115	L111Q (CTG→CAG)	PP_4315	epimerase
Reads supporting (aligned to +/- strand): ref base A (56/52); new base T (4/3); total (60/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 124 total aligned reads displayed.

ACGTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGTGAA  >  NC_002947/4906362‑4906659

aCGTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCcagca                                                                                                                                                       >  2:1124109/1‑150 (MQ=255)

aCGTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCcagca                                                                                                                                                       >  1:1267665/1‑150 (MQ=255)

          cagcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtggac                                                                                                                                             <  1:1675948/150‑5 (MQ=255)

          cagcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAgc                                                                                                                                             <  1:1534826/150‑1 (MQ=255)

           agcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAgcg                                                                                                                                            <  1:1530119/150‑1 (MQ=255)

           agcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGACGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCATCAGGCCATCAgcg                                                                                                                                            >  2:1298188/1‑150 (MQ=255)

            gcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAgcgc                                                                                                                                           <  2:2178459/150‑1 (MQ=255)

              aTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCa                                                                                                                                         <  1:2319920/150‑1 (MQ=255)

               tGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCAc                                                                                                                                        <  2:229631/150‑1 (MQ=255)

                   gTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCt                                                                                                                                    >  1:714970/1‑150 (MQ=255)

                   gTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCt                                                                                                                                    >  1:715249/1‑150 (MQ=255)

                      atccatccCCAAGACATGATCTTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAc                                                                                                                                 <  2:662283/150‑1 (MQ=255)

                      aTCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAc                                                                                                                                 >  1:2409085/1‑150 (MQ=255)

                       tCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtg                                                                                                                                                <  1:438573/134‑2 (MQ=255)

                        cGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAcgc                                                                                                                               <  1:1033481/150‑1 (MQ=255)

                         gATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAcgcg                                                                                                                              >  1:696768/1‑150 (MQ=255)

                         gATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAcgcg                                                                                                                              >  1:508323/1‑150 (MQ=255)

                             accacGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAAc                                                                                                                         >  2:238718/1‑150 (MQ=255)

                               cacGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTc                                                                                                                       >  1:214796/1‑150 (MQ=255)

                               cacGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTc                                                                                                                       >  1:561394/1‑150 (MQ=255)

                                 cGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGc                                                                                                                     <  2:2409085/150‑1 (MQ=255)

                                 cGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGc                                                                                                                     <  2:714970/150‑1 (MQ=255)

                                 cGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGc                                                                                                                     <  2:715249/150‑1 (MQ=255)

                                    cATAATCGTCGCTAAGGTACACCGCCTGCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCAc                                                                                                                  >  2:1726375/1‑150 (MQ=255)

                                     aTAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACt                                                                                                                 <  2:1544643/150‑1 (MQ=255)

                                        aTCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCg                                                                                                              >  2:605698/1‑150 (MQ=255)

                                        aTCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCg                                                                                                              >  1:1042340/1‑150 (MQ=255)

                                         tcgtcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CCACTCGCCACTGCgg                                                                                                             >  2:962884/1‑150 (MQ=255)

                                           gtcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCgggc                                                                                                           >  2:1831047/1‑149 (MQ=255)

                                            tcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTAGTGGGTGTTTGGCGCGGCCGTCCGCGGCCGGCTTGCCATCAGCGGCACGGCTGACGCG‑CAACTCGCCACTGCGGGTa                                                                                                          >  1:1742310/1‑150 (MQ=255)

                                            tcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTa                                                                                                          <  2:451851/150‑1 (MQ=255)

                                               cTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTg                                                                                                       >  2:2026945/1‑150 (MQ=255)

                                                tAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAcgc                                                                                                                               >  2:77576/1‑126 (MQ=255)

                                                tAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTga                                                                                                      >  1:96434/1‑150 (MQ=255)

                                                tAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTga                                                                                                      >  1:1465585/1‑150 (MQ=255)

                                                 aaGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTgaa                                                                                                     <  1:2332861/150‑1 (MQ=255)

                                                  aGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTgaag                                                                                                    >  2:1327921/1‑150 (MQ=255)

                                                  aGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCCCAACATTTGAGGCG‑CAACTCGCCACTGCGGGTATTgaag                                                                                                    >  2:2410375/1‑150 (MQ=255)

                                                   ggTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCCAATCGTCGGGTATTgaagc                                                                                                   <  1:1263100/150‑1 (MQ=255)

                                                      acacCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTgaagcgaa                                                                                                <  2:2096732/150‑1 (MQ=255)

                                                      acacCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTgaagcgaa                                                                                                <  2:508323/150‑1 (MQ=255)

                                                       cacCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTgaagcgaa                                                                                                <  2:1553877/149‑1 (MQ=255)

                                                       cacCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTgaagcgaa                                                                                                >  1:1553877/1‑149 (MQ=255)

                                                        acCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTgaagcgaagc                                                                                              >  1:1200314/1‑150 (MQ=255)

                                                          cGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtgga                                                                                                                                              >  2:490845/1‑98 (MQ=255)

                                                          cGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtgga                                                                                                                                              <  1:490845/101‑4 (MQ=255)

                                                          cGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCAc                                                                                            >  1:2028495/1‑150 (MQ=255)

                                                           gCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACt                                                                                           >  2:1042635/1‑150 (MQ=255)

                                                            ccTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACtt                                                                                          <  1:2004753/150‑1 (MQ=255)

                                                              ttCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTgc                                                                                        >  2:131073/1‑150 (MQ=255)

                                                                  ccTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCt                                                                                                                                    <  1:2329926/103‑1 (MQ=255)

                                                                  ccTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCt                                                                                                                                    >  2:2329926/1‑103 (MQ=255)

                                                                  ccTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCt                                                                                    <  1:1124109/150‑1 (MQ=255)

                                                                        tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGCCCGATACGGGTTGCTGGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAc                                                                              >  1:1226415/1‑150 (MQ=255)

                                                                        tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGc                                                                                                               <  1:1433008/117‑1 (MQ=255)

                                                                        tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAc                                                                              >  1:1898375/1‑150 (MQ=255)

                                                                        tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAc                                                                              >  1:639824/1‑150 (MQ=255)

                                                                              ccAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGtt                                                                        <  1:943545/150‑1 (MQ=255)

                                                                                      tGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAAc                                                                >  2:2267574/1‑150 (MQ=255)

                                                                                                ccGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCAATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAg                                                      >  2:605964/1‑150 (MQ=255)

                                                                                                    tGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGa                                                  >  2:1769987/1‑150 (MQ=255)

                                                                                                        aTGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGCCGCG‑CAACTCGCCACTGCGGGGATTGAAGCGAAGCACTTGCGCCTGCTCACCGAGTTACTCGAACAGCGCTTAAGCCGAGGcc                                              >  1:153784/1‑150 (MQ=255)

                                                                                                        aTGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGcc                                              >  1:284884/1‑150 (MQ=255)

                                                                                                         tGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAAc                                                                <  2:1575820/131‑1 (MQ=255)

                                                                                                          gTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCaa                                            <  1:2075784/150‑1 (MQ=255)

                                                                                                              aCCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCAAGGAAGAAGCG‑CCACTCGCCAATGCGGGAATTGAAGCGAATCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTa                                        >  2:1463302/1‑150 (MQ=255)

                                                                                                                   tACGTGTTGTTTGGCGGCTAAGTCCATGGCCAGCACGCCATCCGCGCCACGGCTGACGCG‑CAACTCGCCACTGCCGGTATTGAAGCGAAGCAATTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATg                                   <  2:320168/150‑1 (MQ=255)

                                                                                                                     cGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAccc                                                                            >  2:1683856/1‑107 (MQ=255)

                                                                                                                     cGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAccc                                                                            <  1:1683856/107‑1 (MQ=255)

                                                                                                                        gttgttTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGc                              >  2:2261809/1‑150 (MQ=255)

                                                                                                                         ttgtttgtCGGGGAAGTCCATGGCCAGCAGG‑CGCCAGCCCTTCTTCGAGAGCGACAACTCGCCACTGCGGGCAGTAAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGca                             <  1:1740928/150‑1 (MQ=255)

                                                                                                                         ttgttTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGca                             >  1:2056498/1‑150 (MQ=255)

                                                                                                                         ttgttTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGca                             <  2:230094/150‑1 (MQ=255)

                                                                                                                           gttTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGcaca                           >  1:2129106/1‑150 (MQ=255)

                                                                                                                            tttGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGTACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAg                          >  2:1485642/1‑150 (MQ=255)

                                                                                                                            tttGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGcacac                          <  2:422846/150‑2 (MQ=255)

                                                                                                                                  ggggAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAcc                                                                             >  2:1372282/1‑93 (MQ=255)

                                                                                                                                  ggggAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAcc                                                                             <  1:1372282/93‑1 (MQ=255)

                                                                                                                                   gggAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACt                   >  1:2418299/1‑150 (MQ=255)

                                                                                                                                    ggAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTCGCGCCTGCTCACCCAGTTGCTCGAACAGCACGGAAGCCGAGTCCAAGGTAGCATGGCCGCACAGGTCGACtt                  <  2:1465585/150‑1 (MQ=255)

                                                                                                                                        gTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCAc              >  2:542000/1‑150 (MQ=255)

                                                                                                                                         tCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAc                                                                              <  2:1906779/85‑1 (MQ=255)

                                                                                                                                         tCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAc                                                                              >  1:1906779/1‑85 (MQ=255)

                                                                                                                                         tCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGCGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACg             >  2:1071913/1‑150 (MQ=255)

                                                                                                                                         tCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACg             >  1:986905/1‑150 (MQ=255)

                                                                                                                                         tCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACg             <  1:131073/150‑1 (MQ=255)

                                                                                                                                         ctgaTGGCCTGCTGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCCAGAAAAACGTCCCCAAGCTAGCATATCCGCACATGGCGACTTACACg             >  2:1204550/4‑150 (MQ=255)

                                                                                                                                           cATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGt           <  1:962884/150‑1 (MQ=255)

                                                                                                                                            aTGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTg          >  2:2444091/1‑150 (MQ=255)

                                                                                                                                            aTGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTg          <  2:2119102/150‑1 (MQ=255)

                                                                                                                                             tGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAg                                           >  1:2009201/1‑116 (MQ=255)

                                                                                                                                             tGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAg                                           <  2:2009201/116‑1 (MQ=255)

                                                                                                                                               gCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTgggg       >  2:377281/1‑150 (MQ=255)

                                                                                                                                               gCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTgggg       <  1:2412853/150‑1 (MQ=255)

                                                                                                                                                ctagcagGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTggggg      >  1:1351247/3‑150 (MQ=255)

                                                                                                                                                 cagcagGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTGGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGt     <  2:1898375/150‑1 (MQ=255)

                                                                                                                                                 cagcagGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGt     <  1:2444091/150‑1 (MQ=255)

                                                                                                                                                  agcagGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAg                                           <  2:637208/111‑1 (MQ=255)

                                                                                                                                                   gcagGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGTGa   <  2:1179630/150‑1 (MQ=255)

                                                                                                                                                    cagGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGTGaa  <  1:1298188/150‑1 (MQ=255)

ACGTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCG‑CAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGTGAA  >  NC_002947/4906362‑4906659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`