BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,918,011	T→C	7.8%	V122A (GTC→GCC) ‡	PP_4328 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,918,011	0	T	C	7.8%	288.2 / 10.7	102	V122A (GTC→GCC) ‡	PP_4328	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (49/45); new base C (4/4); total (53/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

Only 100 of 103 total aligned reads displayed.

TTCAGGCCAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCA  >  NC_002947/4917864‑4918160

ttCAGGCCAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCg                                                                                                                                                     >  1:1733282/1‑150 (MQ=255)

 tCAGGCCAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCCATTATAGAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCgg                                                                                                                                                    <  1:25575/150‑1 (MQ=255)

 tCAGGCCAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCgg                                                                                                                                                    <  2:496070/150‑1 (MQ=255)

   aGGCCAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCa                                                                                                                                                  <  2:2145533/150‑1 (MQ=255)

    ggCCAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCAc                                                                                                                                                 >  1:674234/1‑150 (MQ=255)

     gCCAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCCATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCAcc                                                                                                                                                >  1:677543/1‑150 (MQ=255)

     gCCAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCAcc                                                                                                                                                >  2:2228811/1‑150 (MQ=255)

       cAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCtt                                                                                                                                              >  1:1900008/1‑150 (MQ=255)

        aaCGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTtg                                                                                                                                             >  1:982370/1‑150 (MQ=255)

        aaCGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTtg                                                                                                                                             >  1:1088740/1‑150 (MQ=255)

         aCGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑ACGGTGCCGACCGGCACCTtgc                                                                                                                                            <  1:1209669/150‑1 (MQ=255)

          cGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTtgct                                                                                                                                           >  1:1772443/1‑150 (MQ=255)

               gccagccGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaag                                                                                                                                      <  2:731165/150‑1 (MQ=255)

               gccagccGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaag                                                                                                                                      <  2:779730/150‑1 (MQ=255)

               gccagccGCTGCCCCAGGCCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaag                                                                                                                                      <  1:2280537/150‑1 (MQ=255)

                   gccGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaaggcaa                                                                                                                                  <  1:2228811/150‑1 (MQ=255)

                   ctggCTGCCCTGGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaaggcaa                                                                                                                                  >  2:1435409/4‑150 (MQ=255)

                    tggCTGCCCTGGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaaggcaag                                                                                                                                 >  2:2223097/3‑150 (MQ=255)

                      gCTGCCCTGGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGt                                                                                                                               >  2:1541265/1‑150 (MQ=255)

                           cccAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGTCAGGTCGCCACCCTCACCGATCGGTACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGa                                                                                                                          <  1:1487209/150‑1 (MQ=255)

                           cccAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGa                                                                                                                          <  2:1618198/150‑1 (MQ=255)

                            ccAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGac                                                                                                                         >  2:518944/1‑150 (MQ=255)

                            ccAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGa                                                                                                                          >  2:2432660/1‑149 (MQ=255)

                            ccAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGa                                                                                                                          <  1:2432660/149‑1 (MQ=255)

                               gggCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGaccaa                                                                                                                      <  2:402571/150‑1 (MQ=255)

                                ggCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGaccaac                                                                                                                     <  1:518944/150‑1 (MQ=255)

                                ggCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGaccaac                                                                                                                     <  1:2063797/150‑1 (MQ=255)

                                     ccAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAgct                                                                                                                <  1:2441410/150‑1 (MQ=255)

                                       aGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAgctgc                                                                                                              >  2:787909/1‑150 (MQ=255)

                                        gATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaa                                                                                                                                       <  2:1865518/124‑1 (MQ=255)

                                        gATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaa                                                                                                                                       >  1:1865518/1‑124 (MQ=255)

                                         aTCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGctgctg                                                                                                            <  2:674234/150‑1 (MQ=255)

                                           cACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGc                                                                                                          <  2:1088740/150‑1 (MQ=255)

                                             ccGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGccc                                                                                                        <  1:1177354/150‑1 (MQ=255)

                                                    ccAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCAcc                                                                                                 >  2:2019488/1‑150 (MQ=255)

                                                      aGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCg                                                                                               >  1:1976344/1‑150 (MQ=255)

                                                            aaaGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAAc                                                                                         >  1:1990690/1‑150 (MQ=255)

                                                              aGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCAAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCg                                                                                       >  1:2133717/1‑150 (MQ=255)

                                                                  aTCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCg                                                                                   >  2:2132578/1‑150 (MQ=255)

                                                                   tCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACACAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCgt                                                                                  >  2:1796060/1‑150 (MQ=255)

                                                                     gCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCgtgt                                                                                >  2:2295579/1‑150 (MQ=255)

                                                                     gCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCACCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCgtgt                                                                                >  1:1610222/1‑150 (MQ=255)

                                                                          gCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCg                                                                                               >  2:2012159/1‑130 (MQ=255)

                                                                          gCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCg                                                                                               <  1:2012159/130‑1 (MQ=255)

                                                                               cATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCt                                                                      >  1:1315501/1‑150 (MQ=255)

                                                                               cATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCt                                                                      <  2:2071927/150‑1 (MQ=255)

                                                                               cATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCt                                                                      <  1:2295579/150‑1 (MQ=255)

                                                                                 tgctgcAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACNCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTgg                                                                    >  1:2433523/1‑150 (MQ=255)

                                                                                    tgcAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCa                                                                 <  1:2132578/150‑1 (MQ=255)

                                                                                      cAACAGGCCATCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGc                                                               <  2:1818295/150‑1 (MQ=255)

                                                                                      cAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGTTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGc                                                               <  2:1764679/150‑1 (MQ=255)

                                                                                       aaCAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGcc                                                              >  1:2159226/1‑150 (MQ=255)

                                                                                       aaCAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGcc                                                              <  1:1955642/150‑1 (MQ=255)

                                                                                       aaCAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGA‑ATCACCGCGGGAGCCGGTCGGCACCTTGCGGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGcc                                                              >  1:46219/1‑150 (MQ=255)

                                                                                        aCAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCCCCTTGCTGCAAGGCAAGCTCCTGACCAACCAGCTGCTCGCCCAAGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCt                                                             >  2:158730/1‑150 (MQ=255)

                                                                                         cAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTg                                                            <  2:222017/150‑1 (MQ=255)

                                                                                          aGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGc                                                           <  2:1590347/150‑1 (MQ=255)

                                                                                            gccagcgccagcCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTc                                                         <  1:196073/150‑1 (MQ=255)

                                                                                            gccagcgccagcCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTc                                                         <  1:1541265/150‑1 (MQ=255)

                                                                                             ccagcgccagcCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCa                                                        >  1:485612/1‑150 (MQ=255)

                                                                                             ccagcgccagcCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGACCGGCACCTtgctg                                                                                                                                          >  2:1127708/1‑68 (MQ=37)

                                                                                             ccagcgccagcCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGACCGGCACCTtgctg                                                                                                                                          <  1:1127708/68‑1 (MQ=37)

                                                                                              cagcgccagcCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCaa                                                       >  1:2296091/1‑150 (MQ=255)

                                                                                               agcnccagcCAGGGCGCCACCCCCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAAc                                                      <  1:1492463/150‑1 (MQ=255)

                                                                                               agcgccagcCAGGCCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAAc                                                      <  1:1435409/150‑1 (MQ=255)

                                                                                                    cagccagGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCaa                                                 >  2:579583/1‑150 (MQ=255)

                                                                                                     agccagGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAAc                                                >  1:1047334/1‑150 (MQ=255)

                                                                                                      gccagGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaaggc                                                                                                                                    >  1:1629010/1‑65 (MQ=255)

                                                                                                      gccagGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTgcaaggc                                                                                                                                    <  2:1629010/65‑1 (MQ=255)

                                                                                                        cagGTCACCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAg                                             <  2:1610222/150‑1 (MQ=255)

                                                                                                         agGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAgg                                            >  1:1091100/1‑150 (MQ=255)

                                                                                                               ccACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTg                                      >  2:1792087/1‑150 (MQ=255)

                                                                                                               ccACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTAAACAGCAACCAGGCCGGTg                                      <  1:2019488/150‑1 (MQ=255)

                                                                                                                cACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGc                                     >  1:1036458/1‑150 (MQ=255)

                                                                                                                   ccTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGGCTGCTCAACAGCAACCAGGCCGGTGCcac                                  >  1:8005/1‑150 (MQ=255)

                                                                                                                     tCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTgg                                                                    <  2:1571819/114‑1 (MQ=255)

                                                                                                                     tCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTgg                                                                    >  1:1571819/1‑114 (MQ=255)

                                                                                                                       aCCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTg                              <  2:1733282/150‑1 (MQ=255)

                                                                                                                       aCCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTg                              >  1:921243/1‑150 (MQ=255)

                                                                                                                        cccAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCACCAGCAACCAGGCCGGTGCCACACTGa                             <  1:1520054/150‑1 (MQ=255)

                                                                                                                        cccAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGa                             <  1:2223097/150‑1 (MQ=255)

                                                                                                                         ccAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGTCCAACCGGCCGTGTACCGGGCGCTGGTCAGCATCCTCAATCGCAGCCAACCCGGTGCCACACTGAg                            >  1:525265/1‑150 (MQ=255)

                                                                                                                          cAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGc                           <  2:2159226/150‑1 (MQ=255)

                                                                                                                             cTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATc                        <  2:226247/150‑1 (MQ=255)

                                                                                                                                 acacCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACa                    >  1:864344/1‑150 (MQ=255)

                                                                                                                                     cAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAAc                                                >  2:655652/1‑118 (MQ=255)

                                                                                                                                     cAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAAc                                                <  1:2271298/118‑1 (MQ=255)

                                                                                                                                     cAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAAc                                                >  2:2271298/1‑118 (MQ=255)

                                                                                                                                     cAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAAc                                                <  1:655652/118‑1 (MQ=255)

                                                                                                                                     cAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAgccc                >  2:1763456/1‑150 (MQ=255)

                                                                                                                                     cAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAgccc                >  1:939860/1‑150 (MQ=255)

                                                                                                                                       gC‑AAGGTGCCGACCGGCACCTTGCTGGAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAgcccgc              >  1:2317476/1‑150 (MQ=255)

                                                                                                                                           aGGTGCCGACCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAgcccgcgcc           >  2:172578/1‑150 (MQ=255)

                                                                                                                                            ggTGCCGACCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACt                               <  2:345887/129‑1 (MQ=255)

                                                                                                                                            ggTGCCGACCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACt                               >  1:345887/1‑129 (MQ=255)

                                                                                                                                             gTGCCGGTCTGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGACACCCGGCCAACCGGACTTAGACCGGGCGCTTGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAgcccgcgcccg         >  1:1484569/1‑150 (MQ=255)

                                                                                                                                              tGCCGACCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAgcccgcgcccgc        <  1:172578/150‑1 (MQ=255)

                                                                                                                                                   gTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTc   >  2:2012071/1‑150 (MQ=255)

                                                                                                                                                   gTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTc   <  2:1976344/150‑1 (MQ=255)

                                                                                                                                                    tCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCa  >  1:529060/1‑150 (MQ=255)

TTCAGGCCAACGCCAGCCAGCCGCTGCCCCAGGGCAGCCAGATCACCGTCAGCCAGACCGAAAGCAATCGCCTGGCGATCATGCTGCAACAGGCCAGCGCCAGCCAGGTCGCCACCCTCACCCAGCTGGACACCAGC‑AAGGTGCCGGTCGGCACCTTGCTGCAAGGCAAGGTCCTGACCAACCAGCTGCTGGCCCAGGCACCCGGCCAACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCA  >  NC_002947/4917864‑4918160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`