Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,080,327 A→T 10.1% intergenic (‑144/+24) csrA ← / ← lysC carbon storage regulator/aspartokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,080,3270AT10.1% 325.4 / 23.5 110intergenic (‑144/+24)csrA/lysCcarbon storage regulator/aspartokinase
Reads supporting (aligned to +/- strand):  ref base A (53/45);  new base T (5/6);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 113 total aligned reads displayed.

CAACATAGCTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGATCATCTGG  >  NC_002947/5080178‑5080474
                                                                                                                                                     |                                                                                                                                                   
cAACATAGCTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCaa                                                                                                                                                     <  2:2291279/150‑1 (MQ=255)
  aCATAGCTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGc                                                                                                                                                   >  2:695262/1‑150 (MQ=255)
     tAGCTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCcgc                                                                                                                                                <  1:695262/150‑1 (MQ=255)
     tAGCTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCcgc                                                                                                                                                >  2:2301907/1‑150 (MQ=255)
     tAGCTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCcgc                                                                                                                                                <  2:2321866/150‑1 (MQ=255)
            ctcCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCTTGCCGCGCCCTTc                                                                                                                                         <  2:687486/150‑1 (MQ=255)
            ctcCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTc                                                                                                                                         <  2:2159184/150‑1 (MQ=255)
              ccTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCTTGCCGCGCCCTTcgc                                                                                                                                       <  2:2392499/150‑3 (MQ=255)
              ccTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCa                                                                                                                                       <  2:225062/150‑1 (MQ=255)
               cTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCcgc                                                                                                                                                <  1:682473/140‑1 (MQ=255)
               cTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCcgc                                                                                                                                                >  2:682473/1‑140 (MQ=255)
                ttACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAgg                                                                                                                                     >  1:2441182/1‑150 (MQ=255)
                    gCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCCCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAgc                                                                                                                                 >  1:2123674/1‑150 (MQ=255)
                     cAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAgcg                                                                                                                                >  1:27648/1‑150 (MQ=255)
                      aaaaCATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACTACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAgcgc                                                                                                                               >  1:2143909/1‑150 (MQ=255)
                           aTTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAACCGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTAc                                                                                                                          <  2:2271071/150‑1 (MQ=255)
                             tCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTc                                                                                                                        <  1:1462276/150‑1 (MQ=255)
                               aGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGc                                                                                                                      <  1:353839/150‑1 (MQ=255)
                                  ggTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCt                                                                                                                   <  1:696007/150‑1 (MQ=255)
                                   gTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCTGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTg                                                                                                                  >  1:2417392/1‑150 (MQ=255)
                                    tAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGGCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGt                                                                                                                 >  1:1816206/1‑150 (MQ=255)
                                    tAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGt                                                                                                                 >  2:961502/1‑150 (MQ=255)
                                     aaCAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGTAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTc                                                                                                                <  2:1800628/150‑1 (MQ=255)
                                     aaCAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGcc                                                                                                                     <  1:1807456/145‑1 (MQ=255)
                                     aaCAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGcc                                                                                                                     >  2:1807456/1‑145 (MQ=255)
                                       cAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCgg                                                                                                              >  1:1825804/1‑150 (MQ=255)
                                          tCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa                                                                                                           >  1:1577883/1‑150 (MQ=255)
                                          tCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCATTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa                                                                                                           >  1:1256009/1‑150 (MQ=255)
                                          acTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCACATTACTCGCCCTGTCGGGCa                                                                                                           >  2:945378/2‑150 (MQ=255)
                                           cTTGGATATCGGGCCCGGGTCGCGGACGGCGAGCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAg                                                                                                          >  1:437435/1‑150 (MQ=255)
                                                atatCGGGCCCGGGTCGCGGACTACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCa                                                                                                     <  2:2143909/150‑1 (MQ=255)
                                                atatCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCCCAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCATTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCa                                                                                                     <  2:1256009/150‑1 (MQ=255)
                                                  atCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATc                                                                                                   <  1:1988976/150‑1 (MQ=255)
                                                      ggCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGa                                                                                               <  1:1175588/150‑1 (MQ=255)
                                                       gCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGAt                                                                                              <  2:1349814/150‑1 (MQ=255)
                                                           gggTCGCGGATGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa                                                                                                           <  1:585908/133‑1 (MQ=255)
                                                           gggTCGCGGATGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa                                                                                                           >  2:585908/1‑133 (MQ=255)
                                                            ggTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGacc                                                                                         <  2:642138/150‑3 (MQ=255)
                                                               cgcgGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAAcgcg                                                                                      <  1:779165/150‑1 (MQ=255)
                                                                 cgGACGACGACCATGGCCCCGGTTCCTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGaa                                                                                          <  1:1478394/144‑1 (MQ=255)
                                                                 cgGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGaa                                                                                          >  2:1478394/1‑144 (MQ=255)
                                                                 cgGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGt                                                                                    >  2:141513/1‑150 (MQ=255)
                                                                      gacgacCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATgaa                                                                               <  1:2301907/150‑3 (MQ=255)
                                                                      gacgacCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCa                                                                               <  2:481572/150‑1 (MQ=255)
                                                                      caccacCATCGCCCCGGTTACTCGCCGCACAAGGCAGGACCGGACCTGCGCGGCGCGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCa                                                                               >  2:691923/5‑150 (MQ=255)
                                                                        cgacCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAgc                                                                             >  2:2391806/1‑150 (MQ=255)
                                                                            cATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAgcgcgc                                                                         >  2:486015/1‑150 (MQ=255)
                                                                                 ccccGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACg                                                                    >  1:431345/1‑150 (MQ=255)
                                                                                  cccGGTTACTCGCCGCACGAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACgg                                                                   >  1:346478/1‑150 (MQ=255)
                                                                                   ccGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCACCCTTACAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGc                                                                  >  2:791297/1‑150 (MQ=255)
                                                                                           tCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTcc                                                                                                                                        <  1:1137/72‑1 (MQ=255)
                                                                                           tCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTcc                                                                                                                                        >  2:1137/1‑72 (MQ=255)
                                                                                           tCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCa                                                          <  1:441362/150‑1 (MQ=255)
                                                                                           tCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCa                                                          >  2:1461149/1‑150 (MQ=255)
                                                                                             gccgcACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAg                                                                                                <  1:1133927/110‑1 (MQ=255)
                                                                                             gccgcACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAgg                                                        <  1:353098/150‑1 (MQ=255)
                                                                                               cgcACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTa                                                      >  2:829704/1‑150 (MQ=255)
                                                                                                gcACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTAc                                                     <  2:954525/150‑1 (MQ=255)
                                                                                                    aaGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAg                                                                                                          <  2:220143/93‑1 (MQ=255)
                                                                                                    aaGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAg                                                                                                          >  1:220143/1‑93 (MQ=255)
                                                                                                    aaGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTtct                                                 >  1:1370307/1‑150 (MQ=255)
                                                                                                        caggacaggaCCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTc                                             <  2:2123674/150‑1 (MQ=255)
                                                                                                         aggacaggacCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGGATGCAGCGCGCGTCCGGCCAGCTCCAGGTACTTCTCTTcc                                            >  2:476530/1‑149 (MQ=255)
                                                                                                          ggacaggacCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGa                                           <  1:1451709/150‑1 (MQ=255)
                                                                                                           gacaggacCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAgcgc                                                                                                                               <  2:1485060/65‑1 (MQ=255)
                                                                                                           gacaggacCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAgcgc                                                                                                                               >  1:1485060/1‑65 (MQ=255)
                                                                                                            acaggacCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGc                                         >  2:2070454/1‑150 (MQ=255)
                                                                                                               ggacCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCCAGCCGCCCCCTTCCAGGAAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGcacc                                      >  2:1064227/1‑150 (MQ=255)
                                                                                                                  ccTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa                                                                                                           <  2:1215817/78‑1 (MQ=255)
                                                                                                                  ccTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCa                                                                                                           >  1:1215817/1‑78 (MQ=255)
                                                                                                                  ccTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCaccacc                                   <  2:2441182/150‑1 (MQ=255)
                                                                                                                  ccTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCaccacc                                   >  2:2302533/1‑150 (MQ=255)
                                                                                                                   cTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGAAGAGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCg                                  >  2:919584/1‑150 (MQ=255)
                                                                                                                    tGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCa                                                                               >  2:1259993/1‑104 (MQ=255)
                                                                                                                    tGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCa                                                                               <  1:1259993/104‑1 (MQ=255)
                                                                                                                      cgcgGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGaaa                               <  2:1378599/150‑1 (MQ=255)
                                                                                                                            gAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGa                         >  1:1549934/1‑150 (MQ=255)
                                                                                                                               cAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGAtct                      >  2:831991/1‑150 (MQ=255)
                                                                                                                                aGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGAtctc                     <  2:1816206/150‑1 (MQ=255)
                                                                                                                                 gAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAg                                                                                                          >  2:955622/1‑64 (MQ=255)
                                                                                                                                 gAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAg                                                                                                          <  1:955622/64‑1 (MQ=255)
                                                                                                                                 gAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCg                    >  1:1301314/1‑150 (MQ=255)
                                                                                                                                  aaaCGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTCCTTCTCTTCGAGCACCACCGAAACCTTGATCTCgg                   >  2:1691959/1‑150 (MQ=255)
                                                                                                                                  aaaCGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGa                                           >  2:630872/1‑126 (MQ=255)
                                                                                                                                    cctggAAGGGCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATg                                                                                 >  1:2063468/5‑86 (MQ=38)
                                                                                                                                    cctggAAGGGCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATg                                                                                 <  2:2063468/82‑1 (MQ=37)
                                                                                                                                     cgcgAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTc                                             <  1:236239/121‑1 (MQ=255)
                                                                                                                                       ggAAGGGCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGa                                           >  1:537398/2‑121 (MQ=255)
                                                                                                                                       cgAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTg              <  2:1577883/150‑1 (MQ=255)
                                                                                                                                        gAAGGGCGCGGCAAGCCGCGCCCTTACAGGCAGCGCCTTACTCGCACTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAATgg             >  2:1016844/1‑150 (MQ=255)
                                                                                                                                         aaGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGga            <  2:27648/150‑1 (MQ=255)
                                                                                                                                         aaGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGga            <  1:2391806/150‑1 (MQ=255)
                                                                                                                                            ggCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTAc                                                     <  1:1560622/106‑1 (MQ=255)
                                                                                                                                            ggCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTAc                                                     >  2:1560622/1‑106 (MQ=255)
                                                                                                                                            ggCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCt                            >  1:1446060/1‑131 (MQ=255)
                                                                                                                                            ggCGCGGCTTGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCt                            <  2:1446060/131‑1 (MQ=255)
                                                                                                                                            ggCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGat         >  1:239096/1‑150 (MQ=255)
                                                                                                                                             gcgnGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGatc        >  2:1511767/1‑150 (MQ=255)
                                                                                                                                                 ggCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGATCATCt    <  1:829704/150‑1 (MQ=255)
                                                                                                                                                     aGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTc                                             >  2:292025/1‑105 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAACATAGCTTTCTCCTTACGCAAAACATTCAGGGGTAACAGTCTTGGATATCGGGCCCGGGTCGCGGACGACGACCATGGCCCCGGTTACTCGCCGCACAAGGCAGGACAGGACCTGCGCGGCGAGCAGAAACGCGAAGGGCGCGGCAAGCCGCGCCCTTCCAGGCAGCGCCTTACTCGCCCTGTCGGGCAGGAGCATCCAGATCGAACGCGGTATGCAGCGCGCGTACGGCCAGCTCCAGGTACTTCTCTTCGAGCACCACCGAAACCTTGATCTCGGAAGTGGAGATCATCTGG  >  NC_002947/5080178‑5080474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑