Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,141,748	T→C	7.7%	V12A (GTG→GCG) ‡	PP_4526 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,141,748	0	T	C	7.7%	257.8 / 8.6	91	V12A (GTG→GCG) ‡	PP_4526	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (42/42);  new base C (4/3);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

CACGGCATTCTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGCGCGGTGGACGCC  >  NC_002947/5141607‑5141896                                                                                                                                              |                                                                                                                                                     cACGGCATTCTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCtga                                                                                                                                              <  1:1153798/150‑1 (MQ=255) cACGGCATTCTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATAGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCACGGCGCtgg                                                                                                                                              <  2:2277878/150‑2 (MQ=255) cACGGCATCATCCCAACCAGAACACCTTGGTTATCATTATTCAATTTTATTGAAAAATAAACCACACACAGGTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCtga                                                                                                                                              <  1:525213/150‑1 (MQ=255) cACGGCATACTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCtga                                                                                                                                              >  2:90982/1‑150 (MQ=255)  aCGGCATTCTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCTCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCtgat                                                                                                                                             >  2:818082/1‑150 (MQ=255)  aCGGCATTCTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCtgat                                                                                                                                             >  1:901306/1‑150 (MQ=255)   cGGCATTCTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCtgatg                                                                                                                                            <  2:2303794/150‑1 (MQ=255)   cGGCATTCTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCACGGCGCtggcg                                                                                                                                            <  2:1566216/150‑4 (MQ=255)      cATTCTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGTCTCTGatgatg                                                                                                                                         <  2:344437/150‑1 (MQ=255)           tcCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATgg                                                                                                                                        >  1:2421906/1‑146 (MQ=255)           tcCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATgg                                                                                                                                        <  2:2421906/146‑1 (MQ=255)           tcCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCAt                                                                                                                                    >  1:2360241/1‑150 (MQ=255)           tcCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCAt                                                                                                                                    <  1:2220449/150‑1 (MQ=255)             cAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATgg                                                                                                                                  <  1:1659047/150‑1 (MQ=255)             cAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATgg                                                                                                                                  <  2:1739923/150‑1 (MQ=255)             cAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATgg                                                                                                                                  <  1:1200201/150‑1 (MQ=255)             cAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATgg                                                                                                                                  <  1:2348873/150‑1 (MQ=255)                aaCAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGAAGATGGCCACGGGct                                                                                                                               >  2:1318082/1‑149 (MQ=255)                 aCAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCAt                                                                                                                              <  2:1755296/150‑1 (MQ=255)                                  tcatTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCa                                                                                                             >  1:1012105/1‑150 (MQ=255)                                        ttCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCccgc                                                                                                       >  1:351815/1‑150 (MQ=255)                                        ttCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGACATCGGCCGTTTCGCCCTCACCccgc                                                                                                       >  2:1960366/1‑150 (MQ=255)                                         tCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCccgca                                                                                                      >  1:52869/1‑150 (MQ=255)                                               ttATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCccgcaactgcc                                                                                                <  2:124310/150‑1 (MQ=255)                                                tATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCccgcaactgccg                                                                                               <  1:76758/150‑1 (MQ=255)                                                  ttGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCccgcaactgccgca                                                                                             >  1:166317/1‑150 (MQ=255)                                                        aaTAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGatgatg                                                                                                                                         >  1:1512046/1‑100 (MQ=255)                                                        aaTAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGatgatg                                                                                                                                         <  2:1512046/100‑1 (MQ=255)                                                         aTAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGAtt                                                                                      <  2:2360241/150‑1 (MQ=255)                                                              ccGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGa                                                                                 <  1:923108/150‑1 (MQ=255)                                                                gCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAgg                                                                               <  2:52869/150‑1 (MQ=255)                                                                gCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAgg                                                                               <  1:2396631/150‑1 (MQ=255)                                                                gCCCTAAACTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAgg                                                                               <  2:1933481/150‑1 (MQ=255)                                                                 cccTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAggg                                                                              >  1:583337/1‑150 (MQ=255)                                                                        cTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTc                                                                       <  2:901306/150‑1 (MQ=255)                                                                         tGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCg                                                                      >  2:2439885/1‑150 (MQ=255)                                                                           ccATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGAc                                                                    <  1:2439885/150‑1 (MQ=255)                                                                           ccATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGAc                                                                    <  2:230914/150‑1 (MQ=255)                                                                           ccATCTATCGCTTACCTGCGCAGGAGACATGACACGTCGCCACCGGTCGTACCGGTTGCCAGCGCCGTGGCGATGAGGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGAc                                                                    <  1:2378247/150‑1 (MQ=255)                                                                             aTCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCt                                                                  >  2:1807629/1‑150 (MQ=255)                                                                               cTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGa                                                                <  1:90982/150‑1 (MQ=255)                                                                                tctCGCGTACCTGCGCAGGAGACCCGTCATGTCGCCACTCATCCACCCGCTCGCCAGCGCCGTGGCGCTGATGATGGCCAGGGGCACCGGCCGTTTCGCCCCCCCCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGAc                                                               <  2:619441/148‑1 (MQ=255)                                                                                tATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGAc                                                               >  2:372990/1‑150 (MQ=255)                                                                                  tCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAg                                                             >  2:1277731/1‑150 (MQ=255)                                                                                     cTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCg                                                          >  1:223378/1‑150 (MQ=255)                                                                                         ccTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCgctgg                                                      >  2:51555/1‑150 (MQ=255)                                                                                         ccTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCgctgg                                                      <  2:1999271/150‑1 (MQ=255)                                                                                         ccTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCgctgg                                                      <  1:326554/150‑1 (MQ=255)                                                                                          cTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCgctggg                                                     >  2:222887/1‑150 (MQ=255)                                                                                           tGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCgctgggc                                                    >  2:86008/1‑150 (MQ=255)                                                                                           tGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCgctgggc                                                    <  1:1277731/150‑1 (MQ=255)                                                                                           tGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCgctgggc                                                    >  1:1256256/1‑150 (MQ=255)                                                                                             cccAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGCGGGCCAGTTCGACCTGACAGTCgctgggctg                                                  >  2:201517/3‑150 (MQ=255)                                                                                              gcAGGAGATCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGGTGATGATGGCCATCGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCgctgggctgg                                                 <  2:1306247/150‑1 (MQ=255)                                                                                                       cTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTTATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGcggcgg                                        >  2:487601/1‑150 (MQ=255)                                                                                                        tGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAg                                                                          >  2:987518/1‑115 (MQ=255)                                                                                                        tGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAg                                                                          <  1:987518/115‑1 (MQ=255)                                                                                                        tGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCggcggc                                       <  1:86008/150‑1 (MQ=255)                                                                                                        tGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCggcggc                                       >  1:2430025/1‑150 (MQ=255)                                                                                                         gTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCACTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGcc                                      >  1:74468/1‑150 (MQ=255)                                                                                                            aTGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGAt                                                                                       <  1:1430249/98‑1 (MQ=255)                                                                                                            aTGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGAt                                                                                       >  2:1430249/1‑98 (MQ=255)                                                                                                            aTGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAg                                                                                >  2:2290910/1‑105 (MQ=255)                                                                                                            aTGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAg                                                                                <  1:2290910/105‑1 (MQ=255)                                                                                                                    aCTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTggg                           >  1:317414/1‑150 (MQ=255)                                                                                                                    aCTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTggg                           <  2:465687/150‑1 (MQ=255)                                                                                                                     cTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCTTAGGCATCGGCCGTTTCGCCCTCACCCCTCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGc                          <  1:1960366/150‑1 (MQ=255)                                                                                                                     cTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGc                          <  2:351815/150‑1 (MQ=255)                                                                                                                      tCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGAtt                                                                                      <  2:943084/89‑1 (MQ=255)                                                                                                                      tCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGAtt                                                                                      >  1:943084/1‑89 (MQ=255)                                                                                                                        aTTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTAt                       >  1:681314/1‑150 (MQ=255)                                                                                                                        aTTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTAt                       >  1:1558447/1‑150 (MQ=255)                                                                                                                         ttCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTAtt                      >  2:872918/1‑150 (MQ=255)                                                                                                                           cAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTc                    <  2:583337/150‑1 (MQ=255)                                                                                                                            aaCTGCTCGCCAGCGCCGTGGCGCTGATGCTGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCg                   >  2:2167142/1‑150 (MQ=255)                                                                                                                            aaCTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTAGTTCg                   >  1:110981/1‑150 (MQ=255)                                                                                                                              cTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTg                 <  2:317414/150‑1 (MQ=255)                                                                                                                               tGCTCGCCAGCGCCGTGGCGCTGATGATGGCAATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTgg                <  1:1318082/150‑1 (MQ=255)                                                                                                                                catcatcAGCGCCACGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTggg               >  2:2432224/7‑150 (MQ=255)                                                                                                                                  tCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGgcg             >  2:2103819/1‑150 (MQ=255)                                                                                                                                    gCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGcgcg           >  1:1589684/1‑150 (MQ=255)                                                                                                                                    gCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGcgcg           >  2:1528464/1‑150 (MQ=255)                                                                                                                                     tcAGCGCCACGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCt                              >  1:78525/2‑130 (MQ=255)                                                                                                                                     tcAGCGCCACGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCt                              <  2:78525/129‑1 (MQ=255)                                                                                                                                     tcAGCGCCACGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGCGCgg          >  2:1695832/2‑150 (MQ=255)                                                                                                                                     tcAGCGCCACGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGCGCgg          >  2:943425/2‑150 (MQ=255)                                                                                                                                     ccAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCGTTCGCGTGGGCGCgg          >  1:491391/1‑150 (MQ=255)                                                                                                                                      cAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGCGCGGt         >  2:1947925/1‑150 (MQ=255)                                                                                                                                      cAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGCGCGGt         >  1:2213624/1‑150 (MQ=255)                                                                                                                                          gcCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGCGCGGTGGAc     <  2:617265/150‑1 (MQ=255)                                                                                                                                             gTGGCACTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGCGCGGTGGACGcc  <  2:74468/150‑1 (MQ=255)                                                                                                                                              tGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAgg                                                                               >  2:1197750/1‑72 (MQ=255)                                                                                                                                              tGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAgg                                                                               <  1:1197750/72‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                     CACGGCATTCTCCAAAACAGAACAACTTGATCATCATTATTCAATTTTATTGAAAAATAAACCGCCCTAACCTGCCATCTATCGCTTACCTGCGCAGGAGACCTGTCATGTCGCCACTCATTCAACTGCTCGCCAGCGCCGTGGCGCTGATGATGGCCATGGGCATCGGCCGTTTCGCCCTCACCCCGCAACTGCCGCAACTGATTGCCGAGGGCCAGTTCGACCTGACAGTCGCTGGGCTGGTCGCGGCGGCCAATTACCTGGGCTATTTCGTGGGCGCGGTGGACGCC  >  NC_002947/5141607‑5141896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑