| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,262,544 | T→A | 6.3% | T223T (ACT→ACA) | PP_4638 → | methylenetetrahydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,262,544 | 0 | T | A | 6.3% | 390.3 / 12.2 | 127 | T223T (ACT→ACA) | PP_4638 | methylenetetrahydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (55/64); new base A (5/3); total (60/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
TCACCTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACA > NC_002947/5262396‑5262692 | tCACCTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTg > 1:832007/1‑150 (MQ=255)tCACCTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTg > 1:1329721/1‑150 (MQ=255) cTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCAc < 2:2113060/150‑1 (MQ=255) cTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCACACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCAc < 1:625405/150‑1 (MQ=255) cAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGcca < 2:1934351/150‑1 (MQ=255) gCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGccatc < 2:1897990/150‑1 (MQ=255) gCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGccatc < 2:476607/150‑1 (MQ=255) cTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGccatcc < 2:2032541/150‑1 (MQ=255) tGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGccatcca > 1:1579215/1‑150 (MQ=255) ggggTTCGGTGCCGCCAAGCATGAGGAAGCCATATACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCGCGAC‑CACGTGGCCGGCGTGACTGTCCCGccatccat < 2:956764/150‑1 (MQ=255) ttCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATgcgc > 1:869087/1‑150 (MQ=255) ttCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATgcgc > 2:547551/1‑150 (MQ=255) ttCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACAGTCACGccg < 2:2363762/141‑2 (MQ=255) gATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCTGATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGAc > 2:808869/1‑150 (MQ=255) gATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGAc > 2:2043981/1‑150 (MQ=255) gccgccAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATg > 2:1219011/1‑150 (MQ=255) ccgccAAGCATCACGAAGCCATCCACTTAGTGCACCTGCATCCCTCATCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGc < 2:623243/150‑1 (MQ=255) aGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCACCTCCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAg < 1:726050/150‑1 (MQ=255) gCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGNAGc < 2:1498977/150‑1 (MQ=255) cATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTAAAACACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCg > 1:158977/1‑150 (MQ=255) aTCAGGAAGCCATACACTGGGTGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCgg < 2:45836/150‑1 (MQ=255) aTCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCgg < 2:45555/150‑1 (MQ=255) aaGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACgg < 2:869087/150‑1 (MQ=255) aTACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAg > 2:1391053/1‑150 (MQ=255) aTACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAg > 1:1109385/1‑150 (MQ=255) acacTAGATGCGCCGTCAGGCCCCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCAc < 1:2154306/108‑1 (MQ=255) acacTAGATGCGCCGTCAGGCCCCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCAc > 2:2154306/1‑108 (MQ=255) acTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATgcgc < 2:172478/119‑1 (MQ=255) acTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATgcgc < 2:172216/119‑1 (MQ=255) acTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATgcgc > 1:172478/1‑119 (MQ=255) acTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATgcgc > 1:172216/1‑119 (MQ=255) acTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTccc > 2:47346/1‑149 (MQ=255) tGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAg < 1:2361361/150‑1 (MQ=255) cgcCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCttt < 2:1067925/150‑1 (MQ=255) gcCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTg > 1:384602/1‑150 (MQ=255) ccGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGc < 1:576051/150‑1 (MQ=255) tCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGGAACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCa > 2:1797217/1‑150 (MQ=255) cAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACACATTTAG‑CTGCGTGACTCTCAGGTTGTCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAg < 1:1485416/150‑1 (MQ=255) cAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAg < 2:1329721/150‑1 (MQ=255) ggCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgc < 2:1184643/150‑1 (MQ=255) ggCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgc < 1:890857/150‑1 (MQ=255) gCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgcg > 1:1672632/1‑150 (MQ=255) ccTCACCCAAGCCCATGCTGGCCTGCGCCAGGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgcgc > 1:2276812/1‑150 (MQ=255) ccTCACCCAAGCCCATGCTGGCCTGCCAAAAGAGCCTCACCCACGGGCGCGTGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgcgc < 2:2229625/150‑1 (MQ=255) cTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGCC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGGCATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgcgcg > 1:198723/1‑150 (MQ=255) cTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgcgcg > 2:2188840/1‑150 (MQ=255) tCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgcgcgc > 1:37784/1‑150 (MQ=255) aCCCAAGCCCATGCTGGCCTGCCTCTTACAACTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGAGCGGCGTGACTGTAACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAg > 1:1023796/1‑150 (MQ=255) cAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAgtgt < 1:2067311/150‑1 (MQ=255) aaGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGtt > 1:106305/1‑150 (MQ=255) gCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCGCGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGa < 2:1644251/150‑1 (MQ=255) gCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGa < 1:225033/150‑1 (MQ=255) cccATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGAc < 2:849414/150‑1 (MQ=255) cTGGTCTGCCTCATGAGCCTCATCCCCGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATGCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTg < 2:1192275/150‑1 (MQ=255) ggCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGc < 1:309313/150‑1 (MQ=255) ctgcctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgc < 1:1898614/126‑1 (MQ=255) ctgcctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgc > 2:1898614/1‑126 (MQ=255) ctgcctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCtt > 1:447490/1‑150 (MQ=255) ctgcctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCtt < 1:641163/150‑1 (MQ=255) gcctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGc < 2:1109385/150‑1 (MQ=255) ctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGc > 2:2169339/1‑143 (MQ=255) cATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGAt > 1:1159019/1‑150 (MQ=255) aTGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGtt < 1:37382/129‑1 (MQ=255) aTGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGtt > 2:37382/1‑129 (MQ=255) aTGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGAtt > 2:1914800/1‑150 (MQ=255) gAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCgg > 1:1229191/1‑86 (MQ=255) gCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCg > 2:1323377/1‑150 (MQ=255) ccTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCgg > 2:1054579/1‑150 (MQ=255) cTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGt < 2:2238706/150‑1 (MQ=255) tCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTa < 1:465277/150‑1 (MQ=255) aCCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACa > 1:2154762/1‑150 (MQ=255) aCGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAg > 1:817759/1‑149 (MQ=255) aCGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAg < 2:817759/149‑1 (MQ=255) cGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCt < 2:384602/150‑1 (MQ=255) cgcgcgACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATg < 1:1600887/150‑1 (MQ=255) gcgACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGc > 2:1427929/1‑150 (MQ=255) gcgACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGc < 2:1292807/150‑1 (MQ=255) gaTGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGAAGTGTTGACCGCCCGGCCTTGCAGGTTATCGGTAAAAAGAGGATGGGCCACGc > 1:633182/1‑150 (MQ=255) gCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCgg > 2:738888/1‑150 (MQ=255) cTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgt < 1:1219011/150‑1 (MQ=255) cTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgt < 1:622717/150‑1 (MQ=255) tCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgtg > 2:414453/1‑150 (MQ=255) tCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgtg > 1:1230984/1‑150 (MQ=255) tCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGGACGAAGCTGATGGGCTACGCGGgtg > 2:1192417/1‑150 (MQ=255) c‑cACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCa < 2:1672632/150‑1 (MQ=255) cACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTg > 2:2140499/1‑108 (MQ=255) cACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTg < 1:2140499/108‑1 (MQ=255) ggatggCGTGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTc > 1:509767/5‑150 (MQ=255) gatggCGTGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCa > 1:150810/4‑150 (MQ=255) ccGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTAACAGATGATCCTCACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAg < 1:414453/150‑1 (MQ=255) tggCGTGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAgg > 1:2043922/2‑150 (MQ=255) ggCGTGACTGTCACGCCATCCATGCGCGACATGCCCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAggg < 2:198723/150‑1 (MQ=255) ggCGTGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAggg < 2:150810/150‑1 (MQ=255) gCGTGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgtg < 1:230912/135‑1 (MQ=255) gCGTGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgtg > 2:230912/1‑135 (MQ=255) gTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGtt > 1:1511112/1‑150 (MQ=255) gACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTgg > 2:738044/1‑89 (MQ=255) gACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTgg < 1:738044/89‑1 (MQ=255) gACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTTCAGATTATNGGTACANAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTca < 1:2043981/150‑1 (MQ=255) gACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTca < 1:1427929/150‑1 (MQ=255) | TCACCTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGAC‑CACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACA > NC_002947/5262396‑5262692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |