Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,313,324 C→G 16.1% intergenic (‑595/+119) PP_16SG ← / ← msrQ 16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,313,3240CG16.1% 142.8 / 15.5 54intergenic (‑595/+119)PP_16SG/msrQ16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (21/24);  new base G (4/5);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGC  >  NC_002947/5313175‑5313464
                                                                                                                                                     |                                                                                                                                            
gATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCgccgcc                                                                                                                                              >  1:174696/1‑150 (MQ=255)
     cGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGTCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCcgcgc                                                                                                                                         <  1:1777500/150‑1 (MQ=255)
     cGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCcgcgc                                                                                                                                         <  1:1777461/150‑1 (MQ=255)
           aaGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACCACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCt                                                                                                                                   <  1:6149/150‑1 (MQ=255)
            aggggggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAATTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                  <  2:919576/147‑1 (MQ=255)
            aGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                  <  1:759741/150‑1 (MQ=255)
               ggggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGAt                                                                                                                               <  2:1384632/150‑1 (MQ=255)
                gggCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                                              <  1:139372/150‑1 (MQ=255)
                           aaGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGcc                                                                                                                   >  1:1753184/1‑150 (MQ=255)
                             ggggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCag                                                                                                                 >  1:1392446/1‑150 (MQ=255)
                                     gagaGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCTTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACaa                                                                                                         <  1:743692/150‑1 (MQ=255)
                                     gagaGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACaa                                                                                                         <  2:1392446/150‑1 (MQ=255)
                                      agagGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCGACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACaaa                                                                                                        <  1:1980104/150‑1 (MQ=255)
                                       gagGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAg                                                                                                       <  1:2446114/150‑1 (MQ=255)
                                          ggCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGa                                                                                                                                <  1:516839/122‑1 (MQ=255)
                                          ggCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGa                                                                                                                                >  2:516839/1‑122 (MQ=255)
                                           gCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCgg                                                                                                   <  1:2348139/150‑1 (MQ=255)
                                            cGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCggg                                                                                                  >  2:1696703/1‑150 (MQ=255)
                                                   cTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCtctc                                                                                           <  2:1753184/150‑1 (MQ=255)
                                                   cTTAAATTTCAAGAAAGTGAAAGATAAGGGGAACTACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCTCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCtctc                                                                                           <  1:354591/150‑1 (MQ=255)
                                                         tttCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCTACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGACGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCACTCTCCACAGc                                                                                     >  2:1259458/1‑150 (MQ=255)
                                                         tttCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGc                                                                                     >  1:2144099/1‑150 (MQ=255)
                                                         tttCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGc                                                                                     <  1:2171169/150‑1 (MQ=255)
                                                          ttCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGcc                                                                                    >  1:2026191/1‑150 (MQ=255)
                                                              aGACAGTGACACATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTCTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTg                                                                                <  1:1473441/150‑1 (MQ=255)
                                                               gAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCgg                                                                                                   <  2:538898/130‑1 (MQ=255)
                                                               gAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCgg                                                                                                   >  1:538898/1‑130 (MQ=255)
                                                                 aaGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGCCGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTa                                                                             >  1:397761/1‑150 (MQ=255)
                                                                   gTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAtc                                                                           <  2:2144099/150‑1 (MQ=255)
                                                                        atatAAGGGGAAATCCCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAg                                                                      <  1:1696703/150‑1 (MQ=255)
                                                                             aGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAgg                                                                 >  1:1213728/1‑150 (MQ=255)
                                                                             aGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAgg                                                                 >  1:902024/1‑150 (MQ=255)
                                                                                ggAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGATACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGccc                                                              >  1:1901202/1‑150 (MQ=255)
                                                                                ggAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGccc                                                              >  1:131755/1‑150 (MQ=255)
                                                                                          tATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGccc                                                    >  2:53832/1‑150 (MQ=255)
                                                                                                      cAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCAAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCtt                                                            <  2:1058037/130‑1 (MQ=255)
                                                                                                      cAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCAAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCtt                                                            >  1:1058037/1‑130 (MQ=255)
                                                                                                                    aacaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTcaca                                                                                                                         <  2:1678381/55‑1 (MQ=255)
                                                                                                                    aacaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTcaca                                                                                                                         >  1:1678381/1‑55 (MQ=255)
                                                                                                                     acaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCt                         <  1:629442/150‑1 (MQ=255)
                                                                                                                                      gagaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATCGCTATCTCAATACAAGCCCTTCGCAGCCCCACCCCACCCTCAAACAGCCCTACCCTGCTACCTGCCAACCtag        >  2:353088/1‑148 (MQ=38)
                                                                                                                                      gagaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTg        >  1:1559592/1‑150 (MQ=255)
                                                                                                                                       atctCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTgg       <  2:1225788/147‑1 (MQ=255)
                                                                                                                                          tCGAGCGCCGCGCGGGCGGCGCTCGATCTCATAGGCGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCaa             >  1:2033824/1‑141 (MQ=39)
                                                                                                                                          tCGAGCGCCGCGCGGGCGGCGCTCGATCTCATAGGCGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCaa             <  2:2033824/141‑1 (MQ=39)
                                                                                                                                            gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTgg       >  1:2020398/1‑145 (MQ=255)
                                                                                                                                            gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTgg       <  2:2020398/145‑1 (MQ=255)
                                                                                                                                            gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGAAGACAGAGGCAACCGCTTGAGCCCTCTCCACAGCCATGTCAATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGagat  >  1:1225788/1‑147 (MQ=255)
                                                                                                                                            gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAgc  <  2:2026191/150‑1 (MQ=255)
                                                                                                                                            gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAgc  >  2:597372/1‑150 (MQ=255)
                                                                                                                                            gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCAACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAgc  >  1:2061473/1‑150 (MQ=255)
                                                                                                                                             aGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTg        <  1:615553/143‑1 (MQ=255)
                                                                                                                                             aGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTg        >  2:615553/1‑143 (MQ=255)
                                                                                                                                              gcgcCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCGCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCtt         >  2:1303639/1‑141 (MQ=255)
                                                                                                                                              gcgcCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCtt         <  1:1303639/141‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
GATGTCGCTGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGC  >  NC_002947/5313175‑5313464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑