Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,337,484 C→A 6.5% L296M (CTG→ATG)  PP_4696 → nitrogen regulation protein NR(I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,337,4840CA 6.5% 376.7 / 5.7 117L296M (CTG→ATG) PP_4696nitrogen regulation protein NR(I)
Reads supporting (aligned to +/- strand):  ref base C (51/58);  new base A (4/4);  total (55/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 121 total aligned reads displayed.

TACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTCCTCGCCCGCCAGAGCGCTCGCATC  >  NC_002947/5337336‑5337633
                                                                                                                                                    |                                                                                                                                                     
tACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCt                                                                                                                                                      <  1:896399/150‑1 (MQ=255)
   gCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGaa                                                                                                                                                   >  1:1044643/1‑150 (MQ=255)
     tGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGaaaa                                                                                                                                                 >  1:727675/1‑150 (MQ=255)
         ccTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCt                                                                                                                                             <  2:1044643/150‑1 (MQ=255)
          cTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTg                                                                                                                                            <  1:2098094/150‑1 (MQ=255)
           tCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTgg                                                                                                                                           <  1:2261991/150‑1 (MQ=255)
           tCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTgg                                                                                                                                           <  2:1895573/150‑1 (MQ=255)
             gATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGcc                                                                                                                                         >  2:1693950/1‑150 (MQ=255)
              aTGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTAGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCa                                                                                                                                        >  1:1844838/1‑150 (MQ=255)
                   agtGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCAACGAAAAAAGAAAAACCTGGCCAAggcc                                                                                                                                   <  2:759448/148‑1 (MQ=255)
                    ttGGCGAACTGCCGCTCGACGCCAAGGCTCGCCTGGTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAggccg                                                                                                                                  <  2:1203047/150‑1 (MQ=255)
                    ttGGCGAACTGCCGCTCGAAGCGCAGGATCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAgtccg                                                                                                                                  <  2:421268/150‑1 (MQ=255)
                    ttGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAggccg                                                                                                                                  <  2:2037604/150‑1 (MQ=255)
                      ggCGAACTGCCGCTCGAAGCCCAGGCTCGGCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAggccggc                                                                                                                                <  2:210013/150‑1 (MQ=255)
                       gCGAACTGCCGCTCGAAGCCCAGGCTCAACTACTACGTGTGATGGAGGATGTCGAGATGCGCCTGGGGGGTTGGGTGCAGGCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAggccggcc                                                                                                                               >  2:2104983/1‑150 (MQ=255)
                          aaCTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGCAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATTGCCGCGACCCACCGCGACCTGAAAAAACTGGCCAAGGACGGCCAgg                                                                                                                            >  2:330550/1‑149 (MQ=255)
                                 gCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCTGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTg                                                                                                                                            >  1:358246/1‑127 (MQ=255)
                                 gCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCTGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTg                                                                                                                                            <  2:358246/127‑1 (MQ=255)
                                  cTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTg                                                                                                                                            >  2:953603/1‑126 (MQ=255)
                                  cTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTg                                                                                                                                            <  1:953603/126‑1 (MQ=255)
                                  cTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGaa                                                                                                                    <  2:1859384/150‑1 (MQ=255)
                                  cTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGaa                                                                                                                    <  2:2136429/150‑1 (MQ=255)
                                    cGAAGCCCAGGCTCGCCTGCTGCGTGTGCTTCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGa                                                                                                                  <  1:17767/150‑1 (MQ=255)
                                     gAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGGTCCGTGAAGAc                                                                                                                 >  2:1053900/1‑150 (MQ=255)
                                       atcccATGCTCGCCTGCTGCGTCTGCTGCAGGAATGCGAGATACGCCGTGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACtt                                                                                                               <  1:108695/148‑1 (MQ=255)
                                       aGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACtt                                                                                                               <  2:2068188/150‑1 (MQ=255)
                                        gCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTg                                                                                                              <  2:719546/150‑1 (MQ=255)
                                         cccAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGt                                                                                                             <  2:1399859/150‑1 (MQ=255)
                                          ccAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGAc                                                                                                                 <  1:793640/145‑1 (MQ=255)
                                          ccAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGAc                                                                                                                 >  2:793640/1‑145 (MQ=255)
                                          ccAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTa                                                                                                            >  2:128280/1‑150 (MQ=255)
                                            aGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTAtt                                                                                                          <  1:272212/150‑1 (MQ=255)
                                                  gCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGAc                                                                                                    >  2:1875085/1‑150 (MQ=255)
                                                   ccTGCTGGGGGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACt                                                                                                   >  2:31950/1‑150 (MQ=255)
                                                   ccTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCAGTCAAGACTTGCATCACCGAcc                                                                                                   >  2:1194419/1‑149 (MQ=255)
                                                    ctgctgCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTg                                                                                                  <  2:406129/150‑1 (MQ=255)
                                                     tgctgtGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGc                                                                                                 <  1:882303/150‑1 (MQ=255)
                                                          cGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTc                                                                                            >  2:1448088/1‑150 (MQ=255)
                                                                ctgcAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGcc                                                                                      >  1:1716280/1‑150 (MQ=255)
                                                                ccgcAGGAAGGCGAGATACGCCGGGTAGGTGCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGcc                                                                                      <  1:330550/148‑1 (MQ=255)
                                                                  gcAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGt                                                                                                             <  2:910436/125‑1 (MQ=255)
                                                                     ggAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGa                                                                                                                     >  1:309590/1‑114 (MQ=255)
                                                                     ggAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGa                                                                                                                     <  2:309590/114‑1 (MQ=255)
                                                                     ggAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGaa                                                                                 <  1:128280/150‑1 (MQ=255)
                                                                      gAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGAGCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGTCTTGTATTACCGACTGAACGTCCTCGGCCTGAAg                                                                                >  2:1239724/1‑150 (MQ=255)
                                                                          gCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGc                                                                            <  1:1830978/150‑1 (MQ=255)
                                                                            gagaTACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCg                                                                          >  1:8322/1‑150 (MQ=255)
                                                                                  cGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCACGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCTCTGAAGCTGCCGGCCCTg                                                                    <  2:1844838/150‑1 (MQ=255)
                                                                                      gggTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTa                                                                                                            <  1:2187206/106‑1 (MQ=255)
                                                                                      gggTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTa                                                                                                            >  2:2187206/1‑106 (MQ=255)
                                                                                      gggTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGcgcg                                                                >  1:652921/1‑150 (MQ=255)
                                                                                            gTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGc                                                                               <  2:632034/129‑1 (MQ=255)
                                                                                            gTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGc                                                                               >  1:632034/1‑129 (MQ=255)
                                                                                            gTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGGTCCGTGCAGACTTGTATTACCGACTGCCCGTCATCGCCCTGAAGCTGCCGGCCCTGcgcgcgcggg                                                          >  2:526843/1‑148 (MQ=255)
                                                                                             ttCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTgg                                                         >  1:1442357/1‑150 (MQ=255)
                                                                                             ttCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCACTGAAGCTGCCGGCCCTGCGCTCGCGTgg                                                         >  1:499818/1‑150 (MQ=255)
                                                                                             ttCGGGGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTgg                                                         >  2:2314091/1‑150 (MQ=255)
                                                                                              tCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGccc                                                                                     >  2:580697/1‑121 (MQ=255)
                                                                                              tCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGccc                                                                                     <  1:580697/121‑1 (MQ=255)
                                                                                              tCGGTGCAGTCGCAAAAGGTCGATGTGCGACAGACAAACCTTGTTCAGGTTGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGc                                                        <  1:2376924/150‑1 (MQ=255)
                                                                                               cGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCa                                                       <  2:1442357/150‑1 (MQ=255)
                                                                                                 gTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGc                                                     >  1:2369937/1‑150 (MQ=255)
                                                                                                   gCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGa                                                   <  1:1870182/150‑1 (MQ=255)
                                                                                                   gCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGa                                                   <  1:611392/150‑1 (MQ=255)
                                                                                                   gCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATGACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGa                                                   <  1:1122931/150‑1 (MQ=255)
                                                                                                     aGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACg                                                 <  2:1803188/150‑1 (MQ=255)
                                                                                                          cAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAAc                                            <  1:2104983/150‑1 (MQ=255)
                                                                                                               ggTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGAt                                       >  2:1812855/1‑150 (MQ=255)
                                                                                                                gTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCTACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATc                                      <  2:1661505/150‑1 (MQ=255)
                                                                                                                gTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATc                                      >  1:68241/1‑150 (MQ=255)
                                                                                                                   gATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCAc                                                                                               <  2:1560158/90‑1 (MQ=255)
                                                                                                                   gATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCAc                                                                                               >  1:1560158/1‑90 (MQ=255)
                                                                                                                     tgtgCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGaa                                                                                                                    >  1:1397601/1‑67 (MQ=255)
                                                                                                                       tgCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCg                                                                                                                        <  2:2012324/61‑1 (MQ=255)
                                                                                                                          gcCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGaa                                                                                 >  1:109133/1‑97 (MQ=255)
                                                                                                                          gcCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGaa                                                                                 <  2:109133/97‑1 (MQ=255)
                                                                                                                          gcCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGCAGCTGCCGGCCCTGCGCGAGCGTGGCAGCTACGTTTACGAGATCGCCAACGTCt                            <  2:1727972/150‑1 (MQ=255)
                                                                                                                           ccTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACTTCAAc                                            <  1:2219041/133‑1 (MQ=255)
                                                                                                                           ccTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACTTCAAc                                            >  2:2219041/1‑133 (MQ=255)
                                                                                                                            cTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCTTCGACCCGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTc                          >  1:1593724/1‑150 (MQ=255)
                                                                                                                            cTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTc                          <  2:685421/150‑1 (MQ=255)
                                                                                                                             tGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCNTcc                         <  2:1507216/150‑1 (MQ=255)
                                                                                                                                tCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCGCGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGGGGCAGCGACGTCAACGAGATCGCCCACGCCTCCCTcg                      >  2:1085531/1‑150 (MQ=255)
                                                                                                                                tCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTCCTcg                      >  1:1883718/1‑150 (MQ=255)
                                                                                                                                  gccgcGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGt                                                                                                                       <  2:171781/51‑1 (MQ=255)
                                                                                                                                  gccgcGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGt                                                                                                                       >  1:171781/1‑51 (MQ=255)
                                                                                                                                  gccgcGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTCCTcgcc                    >  2:294210/1‑150 (MQ=255)
                                                                                                                                  gccgcGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGAGTGGCAGCGACGTCAACGAGATCGCCAGCGTCTGCCTcccc                    >  1:622734/1‑150 (MQ=255)
                                                                                                                                    cgcgACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTCCTcgcccg                  >  1:1468889/1‑150 (MQ=255)
                                                                                                                                     gcgACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGAc                                                  >  1:1028331/1‑117 (MQ=255)
                                                                                                                                     gcgACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGAc                                                  <  2:1028331/117‑1 (MQ=255)
                                                                                                                                     gcgACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTCCTcgcccgc                 >  1:1419982/1‑150 (MQ=255)
                                                                                                                                          ccACCGCGACATGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCaa                                 >  1:1896658/1‑129 (MQ=255)
                                                                                                                                          ccACCGCGACATGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCaa                                 <  2:1896658/129‑1 (MQ=255)
                                                                                                                                          ccACCGCGACATGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCaa                                 <  2:1895942/129‑1 (MQ=255)
                                                                                                                                          ccACCGCGACATGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCaa                                 >  1:1893023/1‑129 (MQ=255)
                                                                                                                                          ccACCGCGACATGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCaa                                 >  1:1895942/1‑129 (MQ=255)
                                                                                                                                             ccGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTCCTCGCCCGCCAGAgcgc         <  1:1812855/150‑1 (MQ=255)
                                                                                                                                                   ccTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAAc                                            <  1:1548312/109‑1 (MQ=255)
                                                                                                                                                    cTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTCCTCGCCCGCCAGAGCGCTCGCATc  >  1:266290/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGTGGCAGCGACGTCAACGAGATCGCCAACGTCTTCCTCGCCCGCCAGAGCGCTCGCATC  >  NC_002947/5337336‑5337633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑