Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,651,428 C→A 7.3% intergenic (‑185/+28) PP_4959 ← / ← fba diguanylate cyclase/fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,651,4280CA 7.3% 345.8 / 10.0 109intergenic (‑185/+28)PP_4959/fbadiguanylate cyclase/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (54/47);  new base A (4/4);  total (58/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 109 total aligned reads displayed.

TTGGATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGATGCA  >  NC_002947/5651281‑5651575
                                                                                                                                                   |                                                                                                                                                   
  ggATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCg                                                                                                                                                 <  2:181626/150‑1 (MQ=255)
   gATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCgg                                                                                                                                                <  1:957716/150‑1 (MQ=255)
    aTCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCggg                                                                                                                                               <  2:1824483/150‑1 (MQ=255)
     tCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGt                                                                                                                                              <  1:1322638/150‑1 (MQ=255)
     tCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGt                                                                                                                                              <  1:815295/150‑1 (MQ=255)
     tCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGt                                                                                                                                              >  2:815295/1‑150 (MQ=255)
       cATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGttt                                                                                                                                            <  1:283059/150‑1 (MQ=255)
         ttAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGttttt                                                                                                                                          >  1:1336980/1‑150 (MQ=255)
         ttAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGttttt                                                                                                                                          >  1:1342186/1‑150 (MQ=255)
         ttAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCACATAAAAAAACCCGCAGCGATGCGGGttttt                                                                                                                                          >  1:1818857/1‑150 (MQ=255)
          tAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGtttttt                                                                                                                                         <  2:2037678/150‑2 (MQ=255)
           aGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCa                                                                                                                                        <  1:277218/150‑1 (MQ=255)
            gTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                                       <  2:284038/150‑1 (MQ=255)
             tACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAgg                                                                                                                                      <  2:1751063/150‑1 (MQ=255)
             tACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGtttttttat                                                                                                                                      <  2:1818857/150‑5 (MQ=255)
              aCTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGtttttttatg                                                                                                                                     <  2:185438/150‑6 (MQ=255)
                  tAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                                 <  2:601336/150‑1 (MQ=255)
                  tAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                                 <  2:1658355/150‑1 (MQ=255)
                  tAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                                 >  1:1401186/1‑150 (MQ=255)
                    gACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCt                                                                                                                               <  1:2051033/150‑1 (MQ=255)
                     aCCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCTTCGCTGCGGGTTTTTCAGGGTTACCTg                                                                                                                              >  2:896595/1‑150 (MQ=255)
                       cTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGttttttta                                                                                                                                       <  2:217013/139‑4 (MQ=255)
                       cTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGttttttta                                                                                                                                       >  1:217013/1‑136 (MQ=255)
                        tGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCg                                                                                                                           <  2:1401186/150‑1 (MQ=255)
                         gAGGCTCCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGa                                                                                                                          <  2:1322801/150‑1 (MQ=255)
                          aGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGAt                                                                                                                         >  2:2022291/1‑150 (MQ=255)
                              tGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGt                                                                                                                     >  1:1665903/1‑150 (MQ=255)
                                cAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTg                                                                                                                   >  1:1677037/1‑150 (MQ=255)
                                 aaCACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGttttt                                                                                                                                          <  1:693179/126‑1 (MQ=255)
                                 aaCACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGttttt                                                                                                                                          >  2:693179/1‑126 (MQ=255)
                                   cacGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGAcc                                                                                                                <  2:1336980/150‑1 (MQ=255)
                                   cacGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGAcc                                                                                                                <  2:1342186/150‑1 (MQ=255)
                                        aatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggc                                                                                                           <  1:2022291/150‑1 (MQ=255)
                                          tgcaacCAGAACGCCATCAATCATCAACATGTACGGTAGACGACCAACGGTTGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggcgg                                                                                                         <  1:1542376/150‑1 (MQ=255)
                                           gcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggcggc                                                                                                        <  1:800737/150‑1 (MQ=255)
                                            caatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGcc                                                                                                       >  1:373340/1‑150 (MQ=255)
                                              atCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAgg                                                                                                                                      <  1:1998997/117‑1 (MQ=255)
                                              atCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAgg                                                                                                                                      >  2:1998997/1‑117 (MQ=255)
                                              atCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAg                                                                                                     >  1:2094828/1‑150 (MQ=255)
                                                cAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGtt                                                                                                   <  2:2191354/150‑1 (MQ=255)
                                                cAGAACGCTACGAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGtt                                                                                                   <  1:2204746/150‑1 (MQ=255)
                                                 aGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCAACCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTc                                                                                                  >  2:2412541/1‑150 (MQ=255)
                                                   aaCGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCAc                                                                                                >  1:87978/1‑150 (MQ=255)
                                                            aaTGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCCGTGTCCCTGGGCGGCCAGTTACCCTTTCAGGt                                                                                       >  2:895648/1‑150 (MQ=255)
                                                                aTCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGCCCTTGGCGGCCAGTTCACCTTACAGGTAgcg                                                                                   >  2:960705/1‑150 (MQ=255)
                                                                     aCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGt                                                                              >  1:198860/1‑150 (MQ=255)
                                                                       tGTACGGTAGCCGACTAACGGGCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGAGGCCAGTTCACCTTTCAGGTAGCTCTGGTAc                                                                            >  1:234880/1‑150 (MQ=255)
                                                                        gTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACa                                                                           >  2:1813548/1‑150 (MQ=255)
                                                                        gTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACa                                                                           <  2:1665903/150‑1 (MQ=255)
                                                                         tACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACAt                                                                          <  2:373340/150‑1 (MQ=255)
                                                                         tACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACAt                                                                          <  2:293137/150‑1 (MQ=255)
                                                                                gCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTcc                                                                   <  2:430290/150‑1 (MQ=255)
                                                                                gCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAACCGACGGGGAATTGATAAATGGGGCTTGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTcc                                                                   >  1:2379498/1‑150 (MQ=255)
                                                                                        aCGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGAt                                                           >  1:735679/1‑150 (MQ=255)
                                                                                         cggtcggtCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATc                                                          <  2:2007153/150‑1 (MQ=255)
                                                                                           gtcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAg                                                                                         >  1:840275/1‑117 (MQ=255)
                                                                                           gtcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAg                                                                                         <  2:840275/117‑1 (MQ=255)
                                                                                           gtcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACAt                                                                          <  2:763505/132‑1 (MQ=255)
                                                                                           gtcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACAt                                                                          >  1:763505/1‑132 (MQ=255)
                                                                                              ggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCtt                                                     <  1:1753056/150‑1 (MQ=255)
                                                                                               gtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGAc                                                                                                                 >  1:47189/1‑89 (MQ=255)
                                                                                               gtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGAc                                                                                                                 <  2:47189/89‑1 (MQ=255)
                                                                                                    ccGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCtt                                                                                                              <  2:2328703/87‑1 (MQ=255)
                                                                                                    ccGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCtt                                                                                                              >  1:2328703/1‑87 (MQ=255)
                                                                                                    ccGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCtt                                               >  1:875455/1‑150 (MQ=255)
                                                                                                       gAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGa                                            <  2:1747527/150‑1 (MQ=255)
                                                                                                               tACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGc                                    >  2:871646/1‑150 (MQ=255)
                                                                                                               tACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGc                                    >  1:1611619/1‑150 (MQ=255)
                                                                                                                aCAGGTTTCCATCTCCCATAAAAAACCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGAt                                                           <  1:521599/126‑1 (MQ=255)
                                                                                                                aCAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGAt                                                           >  2:521599/1‑126 (MQ=255)
                                                                                                                aCAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGa                                            >  2:1573998/1‑141 (MQ=255)
                                                                                                                aCAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGa                                            <  1:1573998/141‑1 (MQ=255)
                                                                                                                      ttCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGt                             >  2:2449085/1‑150 (MQ=255)
                                                                                                                           tctcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGa                        >  2:1119334/1‑150 (MQ=255)
                                                                                                                           tctcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGa                        >  2:1649202/1‑150 (MQ=255)
                                                                                                                           tctcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTAAATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGa                        >  2:562970/1‑150 (MQ=255)
                                                                                                                            ctcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTTCCCGCGGTGCCGaa                       <  1:2412541/150‑1 (MQ=255)
                                                                                                                            ctcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGaa                       <  1:1119334/150‑1 (MQ=255)
                                                                                                                            ctcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGaa                       >  1:1767941/1‑150 (MQ=255)
                                                                                                                             tcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGTTCTTCGAGGCATTGCCCGCCGTTCCGaat                      <  2:875455/150‑2 (MQ=255)
                                                                                                                             tcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGATGCCGAAg                      >  2:1309552/1‑150 (MQ=255)
                                                                                                                               ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTc                                              <  1:782281/124‑1 (MQ=255)
                                                                                                                               ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTc                                              >  2:782281/1‑124 (MQ=255)
                                                                                                                               ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGc                    >  2:896298/1‑150 (MQ=255)
                                                                                                                                 aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTc                                                                                           <  1:1112286/77‑1 (MQ=255)
                                                                                                                                 aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTc                                                                                           >  2:1112286/1‑77 (MQ=255)
                                                                                                                                 aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCtt                  >  1:908067/1‑150 (MQ=255)
                                                                                                                                 aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCtt                  >  1:1102780/1‑150 (MQ=255)
                                                                                                                                   aaaaaaaCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCg                <  2:198860/150‑1 (MQ=255)
                                                                                                                                     aaaaaCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTa              >  2:2045580/1‑150 (MQ=255)
                                                                                                                                       aaaCCCGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGTGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAc            >  2:2120173/1‑150 (MQ=255)
                                                                                                                                       aaaCCCGAATCACGGCGGGTTTTTCAGGGGTACCTGTCGATCAGTTGGCGTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAc            >  2:1910489/1‑150 (MQ=255)
                                                                                                                                         aCCCGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgc          >  1:2179244/1‑150 (MQ=255)
                                                                                                                                          cccGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAngcg         <  2:1498009/150‑1 (MQ=255)
                                                                                                                                           ccGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgcgc        >  2:2086425/1‑150 (MQ=255)
                                                                                                                                           ccGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgcgc        <  1:871646/150‑1 (MQ=255)
                                                                                                                                            cGCNTCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgcgcg       >  2:1509528/1‑150 (MQ=255)
                                                                                                                                             gCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGa      <  1:1813548/150‑1 (MQ=255)
                                                                                                                                                tCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGATGc   >  1:389269/1‑150 (MQ=255)
                                                                                                                                                 cGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGATGca  <  2:1611619/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTGGATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGATGCA  >  NC_002947/5651281‑5651575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑