Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,844,163 C→T 5.1% A243A (GCC→GCT)  PP_5122 → GMC family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,844,1630CT 5.1% 356.0 / 6.1 118A243A (GCC→GCT) PP_5122GMC family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (57/55);  new base T (3/3);  total (60/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

Only 100 of 119 total aligned reads displayed.

ATGGGCTGCCCGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAACCT  >  NC_002947/5844017‑5844311
                                                                                                                                                    |                                                                                                                                                    
  gggCTGCCCGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGa                                                                                                                                                 <  1:2451273/150‑1 (MQ=255)
  gggCTGCCCGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGa                                                                                                                                                 <  1:1765507/150‑1 (MQ=255)
     cTGCCCGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGc                                                                                                                                              <  1:704635/150‑1 (MQ=255)
         ccGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAg                                                                                                                                          <  2:1216805/150‑1 (MQ=255)
          cGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAgg                                                                                                                                         <  1:660341/150‑1 (MQ=255)
          cGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAgg                                                                                                                                         <  1:194073/150‑1 (MQ=255)
          cGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAgg                                                                                                                                         <  1:180976/150‑1 (MQ=255)
             tGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCa                                                                                                                                      <  1:2295820/150‑1 (MQ=255)
               aaTGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATa                                                                                                                                    <  1:1260414/150‑1 (MQ=255)
               aaTGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATa                                                                                                                                    <  1:1260652/150‑1 (MQ=255)
                 tGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATaca                                                                                                                                  <  1:2127088/150‑1 (MQ=255)
                 tGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACCAATACCGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGGCGTCCAGGGcctggc                                                                                                                                  <  1:1637366/150‑6 (MQ=255)
                   ccAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACAcc                                                                                                                                >  1:229219/1‑150 (MQ=255)
                     aaaCAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCt                                                                                                                              >  1:110256/1‑150 (MQ=255)
                       aCAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTAc                                                                                                                            <  2:241116/150‑1 (MQ=255)
                         aaTCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCg                                                                                                                          >  1:365583/1‑150 (MQ=255)
                           tCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCAAGCAGCGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCggc                                                                                                                        >  1:2253415/1‑150 (MQ=255)
                           tCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGAGCTTCGCTCACAACGGCGACCACA‑AC‑GATGCGGTCATTGACCAGTCCCTGGACGCCCAGGGCATACACCCTACCggc                                                                                                                        >  1:1740999/1‑150 (MQ=255)
                            cGATGCTGGTGACCCGTATAGCGGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCggca                                                                                                                       <  1:1608305/150‑1 (MQ=255)
                             gATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCggcag                                                                                                                      <  2:2393200/150‑1 (MQ=255)
                             gATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCggcag                                                                                                                      <  2:2393458/150‑1 (MQ=255)
                               tGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGTCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCggcaggc                                                                                                                    >  2:196198/1‑150 (MQ=255)
                                gCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGAGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCggcaggca                                                                                                                   <  1:2183496/150‑1 (MQ=255)
                                 cTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGgcaggcag                                                                                                                  <  2:314606/150‑1 (MQ=255)
                                  tGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACg                                                                                                                                               >  1:1857950/1‑120 (MQ=255)
                                     tGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCa                                                                                                              <  1:2039430/150‑1 (MQ=255)
                                     tGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCa                                                                                                              >  2:825538/1‑150 (MQ=255)
                                         ccGTATACCTGCCACCCTCGAGCAAGGTGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGt                                                                                                          >  2:467691/1‑150 (MQ=255)
                                         ccGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGt                                                                                                          >  1:1415527/1‑150 (MQ=255)
                                         ccGTATACCTGCCACCCTCGAGCAAGATCAACAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTAACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGt                                                                                                          >  1:62695/1‑150 (MQ=255)
                                                ccTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAgg                                                                                                                                         <  2:2001953/112‑1 (MQ=255)
                                                ccTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAgg                                                                                                                                         >  1:2001953/1‑112 (MQ=255)
                                                 cTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCAc                                                                                                  >  1:964583/1‑150 (MQ=255)
                                                 cTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCAc                                                                                                  <  1:320811/150‑1 (MQ=255)
                                                 cTGCCACCCTCGAGCAAGGCGGCGAACTGATGTATCTGGCGCGGACTAATATCTGCTCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCAc                                                                                                  <  2:62695/150‑1 (MQ=255)
                                                     cACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCggcagg                                                                                                                     <  2:1943382/127‑1 (MQ=255)
                                                     cACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCCTGCACGctg                                                                                              <  2:365583/150‑3 (MQ=255)
                                                     cACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCa                                                                                              >  1:447389/1‑150 (MQ=255)
                                                      aCCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCTGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCCTGCACGctga                                                                                             <  2:377024/150‑4 (MQ=255)
                                                        ccTCGAGCAAGGCGGCGACCTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTa                                                                                           <  2:1946254/150‑1 (MQ=255)
                                                        ccTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTa                                                                                           <  2:1415527/150‑1 (MQ=255)
                                                         cTCGAGCAATGCGGCGAACTGCTCTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCATGCCCTGGACGTCAAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAc                                                                                          <  1:505430/150‑1 (MQ=255)
                                                         cTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTTCAGGCACGCCATTAc                                                                                          <  1:1147876/150‑1 (MQ=255)
                                                         cTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAc                                                                                          >  1:1794979/1‑150 (MQ=255)
                                                          tCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAcc                                                                                         >  1:824877/1‑150 (MQ=255)
                                                                  ggcggcGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTa                                                                                           <  1:1174163/140‑1 (MQ=255)
                                                                        gAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCggca                                                                                                                       >  2:887393/1‑106 (MQ=255)
                                                                        gAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCa                                                                                              <  2:123821/131‑1 (MQ=255)
                                                                        gAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCa                                                                                              >  1:123821/1‑131 (MQ=255)
                                                                        gAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGGCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGc                                                                           >  1:1975182/1‑150 (MQ=255)
                                                                        gAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCAGTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCggca                                                                                                                       <  1:887393/106‑1 (MQ=255)
                                                                         aaCTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCg                                                                          <  2:110256/150‑1 (MQ=255)
                                                                           ctgctgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCATGCCCTGGGCGTCCAGGGcctgg                                                                                                                                   >  2:1097494/1‑87 (MQ=38)
                                                                           ctgctgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCATGCCCTGGGCGTCCAGGGcctgg                                                                                                                                   <  1:1097494/91‑5 (MQ=38)
                                                                            tgctgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGcc                                                                                 >  2:940304/1‑140 (MQ=255)
                                                                             gctgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTAt                                                                      <  1:986031/150‑1 (MQ=255)
                                                                              ctgTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATc                                                                     >  1:692688/1‑150 (MQ=255)
                                                                                 tATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAAc                                                                  >  2:2314360/1‑150 (MQ=255)
                                                                                   tCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑CA‑AAATGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAg                                                                >  2:919083/1‑150 (MQ=255)
                                                                                       gcgcTGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCACACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGCCCGGTGGCGGTATCAACAGCCCg                                                            <  2:1185257/150‑1 (MQ=255)
                                                                                         gcgGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGc                                                          >  1:499189/1‑150 (MQ=255)
                                                                                              ccGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCctgc                                                     <  1:708479/150‑1 (MQ=255)
                                                                                                      ttCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGt                                                                                                                >  2:2092887/1‑83 (MQ=255)
                                                                                                        cGCTCACAACGAAGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGctc                                           <  1:1695126/150‑1 (MQ=255)
                                                                                                           tCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACTGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGc                                        >  1:244935/1‑150 (MQ=255)
                                                                                                           tCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGc                                        >  2:1544448/1‑150 (MQ=255)
                                                                                                           tCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGc                                        >  2:1612103/1‑150 (MQ=255)
                                                                                                               aaCGGCGAACGCATACAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCCGTGTGCAGGCACGCCATTACCCCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCcgcg                                    >  2:1145108/1‑150 (MQ=255)
                                                                                                                aCGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCTGCCCTGCTGCTGCGCTCTGCCgcgc                                   <  1:566325/150‑1 (MQ=255)
                                                                                                                  ggCGAGCGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGccc                                 <  2:448543/150‑1 (MQ=255)
                                                                                                                    cGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTAc                                                                                                                            >  2:2340846/1‑57 (MQ=255)
                                                                                                                    cGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTAc                                                                                                                            <  1:2340846/57‑1 (MQ=255)
                                                                                                                      aaCGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCa                                                                                                       >  1:2370258/1‑76 (MQ=255)
                                                                                                                         gCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGc                          >  1:794631/1‑150 (MQ=255)
                                                                                                                           aTTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCAtg                                                                                                                                    >  1:1652866/1‑41 (MQ=255)
                                                                                                                           aTTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCAtg                                                                                                                                    <  2:1652866/42‑2 (MQ=255)
                                                                                                                            ttCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATg                       >  2:1807493/1‑150 (MQ=255)
                                                                                                                            tccagggCCTGGCA‑TG‑TCAGGCTCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGAc                              >  2:1363784/7‑143 (MQ=255)
                                                                                                                            tccAGGGCCTGGCA‑TG‑TCAGGCTCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGAc                              <  1:1363784/141‑1 (MQ=255)
                                                                                                                              cagggCCTGGCA‑TG‑TCAGGCTCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAcctc                                                                                       <  1:1187403/80‑1 (MQ=38)
                                                                                                                              cAGGGCCTGGCA‑TG‑TCAGGCTCTAGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTAcctc                                                                                       >  2:1187403/1‑84 (MQ=37)
                                                                                                                              cAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTTGCCGTTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGTGCCCTACCCTCATGGt                     <  2:938221/150‑1 (MQ=255)
                                                                                                                               agggCCTGGCA‑TG‑TCAGGCTCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTg                                                                             >  1:2061535/4‑93 (MQ=255)
                                                                                                                               agggCCTGGCA‑TG‑TCAGGCTCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTg                                                                             <  2:2061535/90‑1 (MQ=255)
                                                                                                                                   ccTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACTGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCt                <  2:244935/150‑1 (MQ=255)
                                                                                                                                   ccTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCt                >  1:1910005/1‑150 (MQ=255)
                                                                                                                                   ccTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGAACGGTCGCCt                >  1:701277/1‑150 (MQ=255)
                                                                                                                                   ccTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGAAACCTGTGGTCGCCt                >  1:2204815/1‑150 (MQ=255)
                                                                                                                                    cTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTg               <  2:229219/150‑1 (MQ=255)
                                                                                                                                     tGACA‑TG‑CGAGGCCATGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTAAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGCTATCAACCGCCCGGCCCTACTGCTGCGCTCTGCCGCGCCCGACCCGCCTGGGCGCCTgt              >  2:1112784/1‑149 (MQ=255)
                                                                                                                                     tGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTgg              >  1:754600/1‑150 (MQ=255)
                                                                                                                                      gACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTggg             >  1:845986/1‑150 (MQ=255)
                                                                                                                                            g‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGc        <  1:368914/150‑1 (MQ=255)
                                                                                                                                              ccAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAAc                                                                  <  1:2025149/91‑1 (MQ=255)
                                                                                                                                              ccAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAAc                                                                  >  2:2025149/1‑91 (MQ=255)
                                                                                                                                               cAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGa      >  1:2258525/1‑150 (MQ=255)
                                                                                                                                                 ggCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCATGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAAc    <  2:2275878/150‑1 (MQ=255)
                                                                                                                                                 ggCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAAc    >  1:2432680/1‑150 (MQ=255)
                                                                                                                                                  gCCCTGGGCGTCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAAcc   >  1:363534/1‑150 (MQ=255)
                                                                                                                                                   cccTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAACCt  >  1:127769/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATGGGCTGCCCGGTGAATGCCAAACAATCGATGCTGGTGACCCGTATACCTGCCACCCTCGAGCAAGGCGGCGAACTGCTGTATCTGGCGCGGGCCGAGCGCTTCGCTCACAACGGCGAACGCATTCAGAGCCTGACA‑TG‑CCAGGCCCTGGACGCCCAGGGCATACACCCTACCGGCAGGCAGGTCAGCGTGCAGGCACGCCATTACCTCCTGGCCGGTGGCGGTATCAACAGCCCGGCCCTGCTGCTGCGCTCTGCCGCGCCCGACCCGCATGGTCGCCTGGGCAAGCGAACCT  >  NC_002947/5844017‑5844311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑