| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,975,623 | G→T | 6.7% | intergenic (+511/‑633) | PP_5727 → / → yifB | hypothetical protein/ATP‑dependent chelatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,975,623 | 0 | G | T | 6.7% | 180.0 / 2.9 | 60 | intergenic (+511/‑633) | PP_5727/yifB | hypothetical protein/ATP‑dependent chelatase |
| Reads supporting (aligned to +/- strand): ref base G (28/28); new base T (2/2); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01 | |||||||||||
GGTTTTTTAGCAGTGGCCATGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTA > NC_002947/5975474‑5975771 | ggTTTTTTAGCAGTGGCCATGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGagagag < 1:2347479/150‑1 (MQ=255)ggTTGTTTAGCAGTGGCCATGAAGAAAATGTATTACGACAATGTATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATTAGAGCGGAGGATTCTCGagagag < 2:1182013/150‑1 (MQ=255) tttAGCAGTGGCCATGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGaaaa < 1:404776/150‑1 (MQ=255) cAGTGGCCATGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAACTGCGTTTTTGTAACAATCAGAGCGGAGGATTCTCGAGAGAGGAaaataaat < 1:1870644/150‑1 (MQ=255) gTGGCCATGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTATAAATTTGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTc > 1:1308683/1‑150 (MQ=255) tGGCCATGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCa < 2:1064070/150‑1 (MQ=255) tGGCCATGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCa > 1:1446932/1‑150 (MQ=255) tGGCCATGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCa > 1:1480174/1‑150 (MQ=255) tGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGc < 1:2400744/150‑1 (MQ=255) tGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAAt < 1:296343/150‑1 (MQ=255) gACNATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCCGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGt > 2:1504511/1‑150 (MQ=255) gCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAAtt > 2:13359/1‑150 (MQ=255) cATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATta < 2:1480174/150‑1 (MQ=255) ttCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGTTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATAc < 2:737398/150‑1 (MQ=255) aaaaCTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCa > 1:344026/1‑113 (MQ=255) aaaaCTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCa < 2:344026/113‑1 (MQ=255) aaaaCTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACatat > 1:1289304/1‑150 (MQ=255) aaaaCTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACatat > 2:89347/1‑150 (MQ=255) aaaaCTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACatat < 2:1289304/150‑1 (MQ=255) ttGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGATAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAAc < 2:334508/150‑1 (MQ=255) ttGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAAc > 1:332519/1‑150 (MQ=255) tGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAAcc < 1:384050/150‑1 (MQ=255) aTTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTgg < 2:264115/150‑1 (MQ=255) aTTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTgg < 1:89347/150‑1 (MQ=255) aTTGCCAGCAGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTgg < 2:404165/150‑1 (MQ=255) cAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAg < 2:1119208/150‑1 (MQ=255) aGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGc > 1:2131962/1‑145 (MQ=255) aGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGc < 2:2131962/145‑1 (MQ=255) aGCCGAAATAGAACTTAGCTCGATAGAGAGCGGACTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGa > 1:2409049/1‑150 (MQ=255) aGCCGAAAGAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGCGAAAATTTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGa < 1:146442/150‑1 (MQ=255) tAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTAAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGc < 2:80972/150‑1 (MQ=255) gAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGt > 2:1267212/1‑150 (MQ=255) gagagCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATAc < 1:1504511/150‑1 (MQ=255) gagagCGGCCTGTTAAAAATAGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATAc > 1:1210964/1‑150 (MQ=255) agagCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCg > 1:356939/1‑133 (MQ=255) agagCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCg < 2:356939/133‑1 (MQ=255) gCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTAACGAGGGTAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGAcc > 1:662906/1‑150 (MQ=255) ccTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGATGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATCTTGAACCTGGCAAAGATACGATGc < 2:1626754/125‑1 (MQ=255) ccTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGATGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGc > 1:1626594/1‑125 (MQ=255) ccTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGATGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGc < 2:1626594/125‑1 (MQ=255) ccTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGATGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGc > 1:1626754/1‑125 (MQ=255) tGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATtata < 2:1887900/95‑1 (MQ=255) tGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATtata > 1:1887900/1‑95 (MQ=255) tGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGc > 1:709235/1‑150 (MQ=255) gTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCa > 1:2419773/1‑150 (MQ=255) gTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCa < 1:13359/150‑1 (MQ=255) ttAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCaa > 1:1161053/1‑75 (MQ=255) ttAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCaa < 2:1161053/75‑1 (MQ=255) ttAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCaa > 1:947095/1‑150 (MQ=255) aaaTGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACa < 1:1527821/150‑1 (MQ=255) gTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCtata > 1:839123/1‑150 (MQ=255) tttttGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCt > 2:2095227/1‑94 (MQ=255) tttttGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCt < 1:2095227/94‑1 (MQ=255) tAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATAtt > 2:485894/1‑150 (MQ=255) aaaaaTAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCaaaa > 1:715919/1‑64 (MQ=255) aaaaaTAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCaaaa < 2:715919/64‑1 (MQ=255) aaaaaTAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATAttt > 1:1042754/1‑150 (MQ=255) aaGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATAAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATATATAAGATATTTTGCAGt < 2:1446932/150‑1 (MQ=255) tcGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGt > 2:1122857/1‑150 (MQ=255) tcGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGt > 1:1323218/1‑150 (MQ=255) gagagaGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTgg > 2:2453301/1‑150 (MQ=255) gagagaGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTAAAAGTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTgg < 1:258967/150‑1 (MQ=255) agGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCCGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTa < 2:1323218/150‑1 (MQ=255) | GGTTTTTTAGCAGTGGCCATGAAGAAAATGTATTACGACAATGCATTTCCAAAACTTGAATTGCCAGCCGAAATAGAACTTAGCTCGATAGAGAGCGGCCTGTTAAAAATGGGTTTTTGTAAAAATAAGAGCGGAGGATTCTCGAGAGAGGAAAATAAATTCAGCATGCTCCATCAATGCAAAAGTGAAATTATACATATTGAACCTGGCAAAGATACGATGCGTATAAGCGGGCCATACGAAGACCTGCAAAACATCTATAAGATATTTTGCAGTTAAGTGCCTCGGAGTGGCTCTA > NC_002947/5975474‑5975771 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |