Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,989,010 G→C 8.5% intergenic (+248/‑298) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,989,0100GC 8.5% 211.4 / 9.1 72intergenic (+248/‑298)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base G (36/29);  new base C (3/3);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

CGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTC  >  NC_002947/5988880‑5989155
                                                                                                                                   |                                                                                                                                                 
cgcgCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATgg                                                                                                                                >  1:2299455/1‑150 (MQ=255)
         gAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCaaa                                                                                                                       >  2:746407/1‑150 (MQ=255)
         gAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCaaa                                                                                                                       >  2:2000751/1‑150 (MQ=255)
           gCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCaaaaa                                                                                                                     >  2:1846919/1‑150 (MQ=255)
           gCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGAGCCTGGCACAGGAGGGGTTGGCCAGAAACAAACGGAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCaaaaa                                                                                                                     >  1:1945461/1‑150 (MQ=255)
               ccTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTAt                                                                                                                 >  1:1250968/1‑150 (MQ=255)
               ccTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCACCTGGCACAGGAGGGATTGCCCAGAAACAAACGTCAGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTAt                                                                                                                 >  1:956202/1‑150 (MQ=255)
                cTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATc                                                                                                                >  1:1364367/1‑150 (MQ=255)
                cTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGAGCGGCGCTCGATCTCATGGGCGCCCAAAAACCATc                                                                                                                <  2:1274640/150‑1 (MQ=255)
                    cgcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCa                                                                                                            <  2:1364367/150‑1 (MQ=255)
                     gcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGNCAg                                                                                                           <  2:1510337/150‑1 (MQ=255)
                         gCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCAc                                                                                                       >  1:95636/1‑150 (MQ=255)
                           cTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCt                                                                                                     <  1:636220/150‑1 (MQ=255)
                            tCGATATCGCGTCGTACAAACAAGGCGGGCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTg                                                                                                    <  1:2331540/150‑1 (MQ=255)
                               atatCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGaa                                                                                                 <  2:1121138/150‑1 (MQ=255)
                                    gcgTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCAt                                                                                            >  1:908874/1‑150 (MQ=255)
                                         gtACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGc                                                                                       <  2:908874/150‑1 (MQ=255)
                                           acaaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATc                                                                                           >  1:1724773/1‑144 (MQ=255)
                                           acaaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATc                                                                                           <  2:1724773/144‑1 (MQ=255)
                                              aacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAttt                                                                                  >  2:1633700/1‑150 (MQ=255)
                                              aacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAttt                                                                                  >  1:2128460/1‑150 (MQ=255)
                                              aacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAt                                                                                    <  2:974426/148‑1 (MQ=255)
                                              aacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAt                                                                                    >  1:974426/1‑148 (MQ=255)
                                               acaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTc                                                                                 >  2:516465/1‑150 (MQ=255)
                                               acaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTc                                                                                 >  1:1989020/1‑150 (MQ=255)
                                                 aaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCt                                                                               >  1:144239/1‑150 (MQ=255)
                                                   ggCGGTCGCGCGCGCCTGTCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCACAAAAATTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGa                                                                             <  2:1265098/150‑1 (MQ=255)
                                                   ggCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGa                                                                             <  1:1604252/150‑1 (MQ=255)
                                                   ggCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCCCGCGCGgcgc                                                                                                                                            <  2:837937/86‑1 (MQ=17)
                                                   ggCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCCCGCGCGgcgc                                                                                                                                            >  1:837937/1‑86 (MQ=17)
                                                    gCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAt                                                                                    <  2:648319/142‑1 (MQ=255)
                                                    gCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAt                                                                                    >  1:648319/1‑142 (MQ=255)
                                                    gCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACCAGCGCAAGGCACCTGGAAGCCATCCTGCAATTTCCTGAc                                                                            >  1:257614/1‑150 (MQ=255)
                                                    gCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACACGCGACAGCTCC‑CGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGAc                                                                            <  1:746407/150‑1 (MQ=38)
                                                      ggTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGa                                                                          <  2:2293047/150‑1 (MQ=255)
                                                             cgcgcCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTc                                                                   <  2:766671/150‑1 (MQ=255)
                                                                  cTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGGTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgccgc                                                              >  1:325632/1‑150 (MQ=255)
                                                                     gCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCAt                                                           <  1:192071/150‑1 (MQ=255)
                                                                       acaGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGgcgc                                                                                                                            >  1:1878544/1‑83 (MQ=255)
                                                                       acaGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGgcgc                                                                                                                            <  2:1878544/83‑1 (MQ=255)
                                                                             gggATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGaa                                                   <  2:1989020/150‑1 (MQ=255)
                                                                                    gCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAGAACTATCGCCCGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACaa                                            >  2:674268/1‑150 (MQ=255)
                                                                                    gCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCt                                                                               <  2:705694/115‑1 (MQ=255)
                                                                                    gCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCt                                                                               >  1:705694/1‑115 (MQ=255)
                                                                                      cAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACaaaa                                          >  2:835379/1‑150 (MQ=255)
                                                                                       aGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACaaaaa                                         >  1:1255412/1‑150 (MQ=255)
                                                                                       aGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACaaaaa                                         <  1:2446211/150‑1 (MQ=255)
                                                                                           acaaacGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCAt                                     <  1:1066037/150‑1 (MQ=255)
                                                                                           acaaacGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCAt                                     <  1:516465/150‑1 (MQ=255)
                                                                                           acaaacGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCAt                                     <  1:2000751/150‑1 (MQ=255)
                                                                                             aaacGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACCCCACCCCACAAAAAccggct                                   >  2:134144/1‑146 (MQ=38)
                                                                                              aacGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATcac                                  <  2:2128460/150‑1 (MQ=255)
                                                                                                     gAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGt                           <  2:95636/150‑1 (MQ=255)
                                                                                                          ggTTTACCCGAGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAAGCGTCTAAt                      >  1:1798907/1‑150 (MQ=255)
                                                                                                           gTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAACAACCATCACATTTCGACTAAtt                     >  2:1959505/1‑150 (MQ=255)
                                                                                                           gTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAAtt                     >  2:726474/1‑150 (MQ=255)
                                                                                                              tACCCGCGA‑AGCGCCGCGCGGGCGGCGCATGATCTAATTTTCCCGCAATAACTACCGTCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATtcta                  >  1:47825/1‑149 (MQ=255)
                                                                                                                cccGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATtc                    >  1:2042639/1‑146 (MQ=255)
                                                                                                                cccGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATtc                    <  2:2042639/146‑1 (MQ=255)
                                                                                                                 ccGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCGTTGACGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGc               >  1:1379368/1‑150 (MQ=255)
                                                                                                                 ccGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGCACTCACAAAAACCATCACAAATCGTCTAATTCTCAGc               >  2:623154/1‑150 (MQ=255)
                                                                                                                 ccGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGc               >  1:442321/1‑150 (MQ=255)
                                                                                                                   gcgA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTg             >  1:984791/1‑150 (MQ=255)
                                                                                                                     tcgaGCGCCGCCCGCGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCAt                                                           >  2:1438645/4‑103 (MQ=38)
                                                                                                                     tcgaGCGCCGCCCGCGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCAt                                                           <  1:1438645/100‑1 (MQ=255)
                                                                                                                     tcgaGCGCCGCCCGCGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATca                                   >  2:2077122/4‑127 (MQ=255)
                                                                                                                     tcgaGCGCCGCCCGCGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATca                                   <  1:2077122/124‑1 (MQ=255)
                                                                                                                     gA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTggg           >  1:1253560/1‑150 (MQ=255)
                                                                                                                           gccgcGCGGGCGGCGCTCGATCTCCTAGCCGAATAAAAAGTGCCGCCTGGCACCTGGAAGCCATCCTGCAATTTGCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCACCACAAATCGTCTAATTCTCAGCTGGGTCCTa      <  1:1114260/150‑1 (MQ=255)
                                                                                                                            cgccgcgGGCGGCGCTCGATCTCAGGGGCGCGCAAAAACTATCGCCAGGCACCTGCAAGCCATCCTTCAATTTCCTGACGATCACCTCGCTGCCATGACGCGAACTCACAAAAACCATCAGAAATCGTCTAATTCTCAGCTGGGTCCTAc     <  2:956202/147‑1 (MQ=255)
                                                                                                                            ccgcGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCTCCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTAc     <  2:1253560/150‑1 (MQ=255)
                                                                                                                               cgcgGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTc  <  2:325632/150‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                                 
CGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGA‑AGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTC  >  NC_002947/5988880‑5989155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑