BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,169,846	T→A	5.8%	S169C (AGC→TGC)	PP_5408 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,169,846	0	T	A	5.8%	446.6 / 13.2	152	S169C (AGC→TGC)	PP_5408	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (62/80); new base A (3/6); total (65/87)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 154 total aligned reads displayed.

ATTGTCTATGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATAAGAGTAAC  >  NC_002947/6169697‑6169995

aTTGTCTATGTTTTTTCGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCt                                                                                                                                                       <  2:1839288/150‑1 (MQ=255)

  tGTCTATGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTgc                                                                                                                                                     <  1:2337625/150‑1 (MQ=255)

    tCTATGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTgcgc                                                                                                                                                   <  1:1098106/150‑1 (MQ=255)

    tCTATGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgc                                                                                                                                                   <  1:95579/150‑1 (MQ=255)

      tATGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGccc                                                                                                                                                 >  2:814833/1‑150 (MQ=255)

      tATGTTTTTTAGTTTGATTTCGAGAGCACGTAGCGTCGTGTCAAGGGCAAGGTGTTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgcaa                                                                                                                                                 <  2:857389/150‑3 (MQ=255)

       aTGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgcaag                                                                                                                                                <  1:781154/150‑4 (MQ=255)

        tGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGc                                                                                                                                               >  1:1870354/1‑150 (MQ=255)

            ttttAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACa                                                                                                                                           >  1:328760/1‑150 (MQ=255)

              ttAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCGACATc                                                                                                                                         <  1:814833/150‑1 (MQ=255)

              ttAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATc                                                                                                                                         <  1:551297/150‑1 (MQ=255)

                 gTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGc                                                                                                                                      <  2:1561899/150‑1 (MQ=255)

                   ttGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTc                                                                                                                                    <  1:2420049/150‑1 (MQ=255)

                          cGAGCACCCGTAGAACCGTTTCAAGTGCACGGTGGTCCCCAAGGCCCCACAGCCGTCCGTAGAATTGTGCCAGACGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTa                                                                                                                             <  1:2377733/150‑1 (MQ=255)

                           gAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTAc                                                                                                                            >  1:833807/1‑150 (MQ=255)

                             gCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCCATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCt                                                                                                                          <  2:2086525/150‑1 (MQ=255)

                             gCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCt                                                                                                                          <  2:328760/150‑1 (MQ=255)

                             gCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCt                                                                                                                          <  2:771394/150‑1 (MQ=255)

                                cccGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGa                                                                                                                       <  2:146960/150‑1 (MQ=255)

                                 ccGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTg                                                                                                                         <  1:525465/147‑1 (MQ=255)

                                 ccGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGAc                                                                                                                      >  1:2176608/1‑150 (MQ=255)

                                  cGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACa                                                                                                                     <  2:96547/150‑1 (MQ=255)

                                  cGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACa                                                                                                                     <  1:1533277/150‑1 (MQ=255)

                                     aGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACagag                                                                                                                  <  1:1162281/150‑1 (MQ=255)

                                       aaCCGTTTCAAGTTCAAGGTGGTGCGCAAGGACGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATTCCAATGGGTCCCTGTTTGCCGTAGGCCTTGCGCTGCCTAGGCTGCGTCCGTAACATCCGCTCCACATTACCTGGACAGAGTg                                                                                                                <  2:1076486/150‑1 (MQ=255)

                                       aaCCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCt                                                                                                                          >  1:1144828/1‑140 (MQ=255)

                                       aaCCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCt                                                                                                                          <  2:1144828/140‑1 (MQ=255)

                                       aaCCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTg                                                                                                                <  1:1169897/150‑1 (MQ=255)

                                            tttCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCAt                                                                                                           >  2:1356139/1‑150 (MQ=255)

                                            tttCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGCACAGAGTGGCCAt                                                                                                           <  2:433621/150‑1 (MQ=255)

                                               cAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATcgc                                                                                                        <  1:20438/150‑1 (MQ=255)

                                                  gTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCgag                                                                                                     >  2:1571241/1‑150 (MQ=255)

                                                       aGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAAt                                                                                                >  1:776407/1‑150 (MQ=255)

                                                       aGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAAt                                                                                                >  1:806983/1‑150 (MQ=255)

                                                        ggtggtGCTCAAGAAGGCAGATCCTTGCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAAGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATg                                                                                               >  1:9693/1‑150 (MQ=255)

                                                          tggtgCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgc                                                                                             >  1:297779/1‑150 (MQ=255)

                                                          tggtgCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgc                                                                                             >  1:1726035/1‑150 (MQ=255)

                                                           ggtgCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgct                                                                                            <  2:886903/150‑1 (MQ=255)

                                                            gtgCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgctg                                                                                           >  2:1417992/1‑150 (MQ=255)

                                                             tgCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgctgc                                                                                          >  1:713117/1‑150 (MQ=255)

                                                               cTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTc                                                                                        <  2:1726035/150‑1 (MQ=255)

                                                               cTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTc                                                                                        <  2:1725835/150‑1 (MQ=255)

                                                                tCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTca                                                                                       >  2:505535/1‑150 (MQ=255)

                                                                tCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTca                                                                                       >  2:848234/1‑150 (MQ=255)

                                                                tCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTca                                                                                       >  2:2247671/1‑150 (MQ=255)

                                                                 cAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATc                                                                                                                                         >  1:1601966/1‑99 (MQ=255)

                                                                 cAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTca                                                                                       <  2:919166/149‑1 (MQ=255)

                                                                  aaGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTcaca                                                                                     <  1:1710788/150‑1 (MQ=255)

                                                                  aaGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTcaca                                                                                     >  2:639393/1‑150 (MQ=255)

                                                                  aaGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgcaagg                                                                                                                                               <  2:527979/92‑5 (MQ=255)

                                                                     gaggCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTTCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGGGGCCATCGCGAGAAAATGgctgct                                                                                         >  2:362145/1‑143 (MQ=255)

                                                                     gaggCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCTCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgctgct                                                                                         <  1:362145/143‑1 (MQ=255)

                                                                     gaggCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGaa                                                                                  >  2:282494/1‑150 (MQ=255)

                                                                      aggCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATgtcgg                                                                                           >  1:1074968/1‑136 (MQ=255)

                                                                      aggCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgctg                                                                                           <  2:1074968/140‑1 (MQ=255)

                                                                       ggCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACt                                                                                <  1:2005694/150‑1 (MQ=255)

                                                                        gCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTg                                                                               <  1:2356787/150‑1 (MQ=255)

                                                                         cAGATCCTTCCGTATAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGTGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTgg                                                                              <  1:988426/150‑1 (MQ=255)

                                                                         cAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTgg                                                                              <  1:1131817/150‑1 (MQ=255)

                                                                         cAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTgg                                                                              <  2:806983/150‑1 (MQ=255)

                                                                         cAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCCATTTCAAACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTgg                                                                              <  1:1356471/150‑1 (MQ=255)

                                                                            aTCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATTGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgcaagg                                                                                                                                               <  1:1642364/82‑5 (MQ=38)

                                                                            aTCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATTGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgcaagg                                                                                                                                               >  2:1642364/1‑78 (MQ=38)

                                                                             tCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGt                                                                          >  2:232094/1‑150 (MQ=255)

                                                                               cTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTAc                                                                        >  1:480776/1‑150 (MQ=255)

                                                                                ttCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACg                                                                       >  2:2144759/1‑150 (MQ=255)

                                                                                 tCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACga                                                                      <  2:776407/150‑1 (MQ=255)

                                                                                   cGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACgaga                                                                    <  2:1766831/150‑1 (MQ=255)

                                                                                   cGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACgaga                                                                    <  2:2176608/150‑1 (MQ=255)

                                                                                     tAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGc                                                                  <  1:1417992/150‑1 (MQ=255)

                                                                                            gtgCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCAGCGCCGGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGa                                                           >  2:35303/1‑150 (MQ=255)

                                                                                                cAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGtt                                                       >  1:596441/1‑150 (MQ=255)

                                                                                                   ccGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCga                                                                                                      >  1:711390/1‑100 (MQ=255)

                                                                                                   ccGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCga                                                                                                      <  2:711390/100‑1 (MQ=255)

                                                                                                        aTGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTcg                                               >  2:1858644/1‑150 (MQ=255)

                                                                                                         tGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTcgc                                              >  1:848547/1‑150 (MQ=255)

                                                                                                         ggCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGATGACTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTcgc                                              <  2:2344411/149‑1 (MQ=255)

                                                                                                            cAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGca                                           >  1:2069996/1‑150 (MQ=255)

                                                                                                            cAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGca                                           >  1:2070053/1‑150 (MQ=255)

                                                                                                             aaTGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTgg                                                                              <  2:2051343/114‑1 (MQ=255)

                                                                                                             aaTGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTgg                                                                              >  1:2051343/1‑114 (MQ=255)

                                                                                                                gggCCCCTGCTGGCCGTAGGCCTTGCGCCGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGcacaca                                       <  2:1963703/150‑1 (MQ=255)

                                                                                                                     ccTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTAc                                  <  2:1339175/150‑1 (MQ=255)

                                                                                                                        gTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACAtg                               <  1:16810/150‑1 (MQ=255)

                                                                                                                         ttGGCGGTTGTCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACAtgt                              >  2:1912967/1‑150 (MQ=255)

                                                                                                                           ggCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTgg                            <  2:255478/150‑1 (MQ=255)

                                                                                                                           ggCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTgg                            <  2:1140754/150‑1 (MQ=255)

                                                                                                                           ggCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCGCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTgg                            <  1:509465/150‑1 (MQ=255)

                                                                                                                            gCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTggg                           <  1:57216/150‑1 (MQ=255)

                                                                                                                               gTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATACGCTCAACGTTGTGTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGa                        >  1:1616369/1‑150 (MQ=255)

                                                                                                                                  ggCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGa                     <  1:1858644/150‑1 (MQ=255)

                                                                                                                                   gCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTgg                            >  2:344778/1‑142 (MQ=255)

                                                                                                                                        gcgcTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTgg                            <  1:1733648/137‑1 (MQ=255)

                                                                                                                                          gcTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCa             >  2:1657426/1‑150 (MQ=255)

                                                                                                                                                tAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACga                                                                      >  1:1820973/1‑87 (MQ=255)

                                                                                                                                                tAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACga                                                                      <  2:1820973/87‑1 (MQ=255)

                                                                                                                                                tAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATAaga       >  1:243934/1‑150 (MQ=255)

                                                                                                                                                tAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATAaga       <  2:1616369/150‑1 (MQ=255)

                                                                                                                                                tAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATAaga       <  1:1474276/150‑1 (MQ=255)

                                                                                                                                                     tGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGTGGGTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATAAGAGTAAc  <  1:2124238/150‑1 (MQ=255)

ATTGTCTATGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATAAGAGTAAC  >  NC_002947/6169697‑6169995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`