BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	944,837	T→C	5.3%	E263G (GAG→GGG)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	944,837	0	T	C	5.3%	335.6 / 5.6	115	E263G (GAG→GGG)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand): ref base T (44/63); new base C (3/3); total (48/67)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01

Only 100 of 116 total aligned reads displayed.

CCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTCGCTGTTG  >  NC_002947/944690‑944986

ccAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCg                                                                                                                                                     >  2:1895594/1‑150 (MQ=255)

   gCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCAGTCGCTTTGTGTTAGCTGCAAGGTAATGCTGAACTGGCCGTGTTCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCa                                                                                                                                                  <  1:2378812/150‑1 (MQ=255)

    ccGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCAc                                                                                                                                                 <  2:467601/150‑1 (MQ=255)

      gcgTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGTGCCGAGCGCGGCTCGa                                                                                                                                               <  2:1172379/150‑1 (MQ=255)

      gcgTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCAAGGAATACGCCACCTGCGCCTCGCACGGAAACCGTCGTGCCGAGCCCGGCTCGa                                                                                                                                               <  1:1995459/150‑1 (MQ=255)

        gTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGTGCCGAGCCCGGCTCGAcg                                                                                                                                             <  1:260901/150‑2 (MQ=255)

         tCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCg                                                                                                                                            <  1:2209504/150‑1 (MQ=255)

            gcggcgCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATCCGCCCCCTGCCCCCCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTa                                                                                                                                         <  1:1232638/150‑1 (MQ=255)

             cggcgCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTaa                                                                                                                                        <  1:2187822/150‑1 (MQ=255)

             cggcgCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTaa                                                                                                                                        >  1:2417501/1‑150 (MQ=255)

                 gcTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACTACCGGTAAGGgt                                                                                                                                    <  2:2180492/150‑1 (MQ=255)

                 gcTGATCTCCAGGGCCTGGCCGTCGCTTTGTCGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCCAGCCGGGCTCGGCACGACCGGTAAGGgt                                                                                                                                    <  2:1250389/150‑1 (MQ=255)

                   tGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGgtgt                                                                                                                                  <  2:1576987/150‑1 (MQ=255)

                    gATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGtt                                                                                                                                 <  2:1118466/150‑1 (MQ=255)

                      tctcCAGGGCCTGGCCGTCGCTTTGTGGCGGGTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTAc                                                                                                                               >  2:2212500/1‑150 (MQ=255)

                        tcCAGGGCCTGGCCGTCGCTTTGTGGCGGTGGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCGATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTAccc                                                                                                                             >  1:1775105/1‑150 (MQ=255)

                         ccAGGGCCTGGCCGTCGCTTTGTGGCGCTATCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCt                                                                                                                            >  1:63061/1‑150 (MQ=255)

                          cAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGTGCCGAGCCCGGc                                                                                                                                                   <  1:1538063/126‑1 (MQ=255)

                               ccTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCAt                                                                                                                      <  2:2079565/150‑1 (MQ=255)

                                   gCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGGACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGAt                                                                                                                  <  2:184470/150‑1 (MQ=255)

                                   gCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGAt                                                                                                                  <  2:237022/150‑1 (MQ=255)

                                   cccGTCGCTTTGGGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCAAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGAt                                                                                                                  <  2:1158109/149‑1 (MQ=255)

                                    ccGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATa                                                                                                                 <  1:908717/150‑1 (MQ=255)

                                        cGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCa                                                                                                             <  1:2013278/150‑1 (MQ=255)

                                           tttGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGAt                                                                                                                  >  1:2042905/1‑142 (MQ=255)

                                           tttGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGAt                                                                                                                  <  2:2042905/142‑1 (MQ=255)

                                                 gCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGt                                                                                                                                          <  2:466318/112‑1 (MQ=255)

                                                 gCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGt                                                                                                                                          >  1:466318/1‑112 (MQ=255)

                                                 gCGGTGGCAGGGTAATGCTTAACTGGCCGGCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTTTTACCCTGCTCATTGATAGCCAGgtcggtcg                                                                                                    <  2:884372/150‑1 (MQ=255)

                                                   ggTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGgc                                                                                                  <  1:1849144/150‑1 (MQ=255)

                                                     ttGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGgcgc                                                                                                <  2:252993/150‑1 (MQ=255)

                                                     ttGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGgcgc                                                                                                <  2:1930926/150‑1 (MQ=255)

                                                       gCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCa                                                                                              >  2:1609506/1‑150 (MQ=255)

                                                          gggTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtg                                                                                           <  2:285103/150‑1 (MQ=255)

                                                          gggTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtg                                                                                           <  2:823986/150‑1 (MQ=255)

                                                           ggcaaTGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATCAGTCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGGAAGGGGGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtgg                                                                                          >  1:1241653/4‑150 (MQ=255)

                                                            gTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtggt                                                                                         >  2:248409/1‑150 (MQ=255)

                                                            gTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtggt                                                                                         <  2:596881/150‑1 (MQ=255)

                                                             tAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtggtg                                                                                        <  2:2031198/150‑1 (MQ=255)

                                                             tAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTCACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtggtg                                                                                        <  1:115247/150‑1 (MQ=255)

                                                                 gCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGt                                                                                    <  1:1895594/150‑1 (MQ=255)

                                                                   tGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGGAGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTg                                                                                  >  1:288588/1‑150 (MQ=255)

                                                                   tGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTg                                                                                  >  1:2224346/1‑150 (MQ=255)

                                                                     aaCTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCg                                                                                >  1:2181824/1‑150 (MQ=255)

                                                                     aaCTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCg                                                                                >  2:1861437/1‑150 (MQ=255)

                                                                       cTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGNGTc                                                                              <  2:1505573/150‑1 (MQ=255)

                                                                        tGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCAt                                                                                                                      >  1:2199362/1‑109 (MQ=255)

                                                                        tGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCAt                                                                                                                      <  2:2199362/109‑1 (MQ=255)

                                                                          gCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCg                                                                                                                                                     <  1:1268074/76‑1 (MQ=255)

                                                                          gCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCg                                                                                                                                                     >  2:1268074/1‑76 (MQ=255)

                                                                             gTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGc                                                                                 <  2:1809974/141‑1 (MQ=255)

                                                                             gTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGc                                                                                 >  1:1809974/1‑141 (MQ=255)

                                                                             gTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGc                                                                        >  1:1012470/1‑150 (MQ=255)

                                                                                  cACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGa                                                                   <  1:954807/150‑1 (MQ=255)

                                                                                   aCCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAggtcg                                                                                                        <  1:1145367/112‑1 (MQ=255)

                                                                                    ccGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACa                                                                 >  2:1887961/1‑150 (MQ=255)

                                                                                           ccGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAg                                                          >  1:1747650/1‑150 (MQ=255)

                                                                                             gCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAATGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGgc                                                        >  1:960884/1‑150 (MQ=255)

                                                                                             gCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGgc                                                        >  1:222107/1‑150 (MQ=255)

                                                                                                 tGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCg                                                    >  2:2406634/1‑150 (MQ=255)

                                                                                                    ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCgaga                                                 >  1:1907129/1‑150 (MQ=255)

                                                                                                    ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCgaga                                                 >  2:1919796/1‑150 (MQ=255)

                                                                                                       gCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCggtaatg                                              <  1:1887961/150‑7 (MQ=255)

                                                                                                       gCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACAt                                              <  1:1919796/150‑1 (MQ=255)

                                                                                                       gCAATACGCCACCTGCGCCACGCACGGAAACCGTCTAGCCTGGCTCGGCACGACCGGTAAGATAGTTGCACCGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACAt                                              <  1:1861437/150‑1 (MQ=255)

                                                                                                          aTACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCAtt                                                                                                                     <  2:1572225/76‑1 (MQ=255)

                                                                                                          aTACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCAtt                                                                                                                     >  1:1572225/1‑76 (MQ=255)

                                                                                                          aTACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTAc                                           <  2:2181824/150‑1 (MQ=255)

                                                                                                          aTACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTAc                                           <  2:1907129/150‑1 (MQ=255)

                                                                                                           tACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGc                                                                                                                                                   >  1:2106125/1‑45 (MQ=255)

                                                                                                           tACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGc                                                                                                                                                   <  2:2106125/45‑1 (MQ=255)

                                                                                                            aCGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACcg                                         <  2:2302903/150‑1 (MQ=255)

                                                                                                              gCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgt                                                                                            >  1:2309320/1‑97 (MQ=255)

                                                                                                              gCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACcggc                                       >  2:2449054/1‑150 (MQ=255)

                                                                                                               ccACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACcggcg                                      >  2:750385/1‑150 (MQ=255)

                                                                                                                      cgcCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCgtcag                               >  1:232100/1‑150 (MQ=255)

                                                                                                                          tCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGtcagtcag                           >  2:469027/1‑150 (MQ=255)

                                                                                                                           cGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAATGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAgg                          <  2:960884/150‑1 (MQ=255)

                                                                                                                              aCGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTg                       <  1:883572/150‑1 (MQ=255)

                                                                                                                                ggAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGAc                     >  2:2122566/1‑150 (MQ=255)

                                                                                                                                ggAAACCGTCGAGCCGGGCGCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGGGAc                     >  2:1403047/1‑150 (MQ=255)

                                                                                                                                   aaCCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATc                  <  2:2417501/150‑1 (MQ=255)

                                                                                                                                      cGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAg               <  1:1974334/150‑1 (MQ=255)

                                                                                                                                      cGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAg               >  2:92324/1‑150 (MQ=255)

                                                                                                                                      cGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAg               <  1:638538/150‑1 (MQ=255)

                                                                                                                                       gTCGTGCCGAGCCCGGCTCGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAgg              >  2:1709866/1‑150 (MQ=255)

                                                                                                                                        tCGTGCCGAGCCCGGCTCGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCa                                                                       >  1:2277110/1‑92 (MQ=255)

                                                                                                                                        tCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGAc                                                <  1:2306143/115‑1 (MQ=255)

                                                                                                                                        tCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGAc                                                >  2:2306143/1‑115 (MQ=255)

                                                                                                                                           ggCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGtttt          <  1:1271328/149‑1 (MQ=255)

                                                                                                                                            gCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTc         <  2:1747650/150‑1 (MQ=255)

                                                                                                                                            gCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTc         <  1:92324/150‑1 (MQ=255)

                                                                                                                                              cGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGCCGTCAGTCAGGGTGACATCAAGGTTTTCGc       >  2:94496/1‑150 (MQ=255)

                                                                                                                                               gggCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTCGCt      >  1:1942330/1‑150 (MQ=255)

                                                                                                                                                ggCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTCGCTg     <  2:222107/150‑1 (MQ=255)

                                                                                                                                                ggCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTCGCTg     >  1:140667/1‑150 (MQ=255)

                                                                                                                                                ggCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTCGCTg     <  1:2449054/150‑1 (MQ=255)

                                                                                                                                                 gCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTCGCTGt    <  1:248409/150‑1 (MQ=255)

                                                                                                                                                 gCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGCCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGCCATTACCGGCGGCGTCAGTCAGGGTGACATCCAGGTTTTCGCTGt    >  2:387209/1‑150 (MQ=255)

                                                                                                                                                   tCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTCGCTGTTg  >  2:1384467/1‑150 (MQ=255)

CCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACAGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAGGTTTTCGCTGTTG  >  NC_002947/944690‑944986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`