Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	991,560	T→C	6.7%	I60T (ATC→ACC)	der →	GTPase Der

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	991,560	0	T	C	6.7%	255.5 / 6.1	92	I60T (ATC→ACC)	der	GTPase Der
Reads supporting (aligned to +/- strand):  ref base T (47/37);  new base C (2/4);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

GAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTGCGCAAGC  >  NC_002947/991414‑991706                                                                                                                                                   |                                                                                                                                                   gAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCAc                                                                                                                                                 <  2:2192046/150‑1 (MQ=255)    cGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCgg                                                                                                                                              <  1:113897/150‑1 (MQ=255)     gTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGt                                                                                                                                             >  2:1828826/1‑150 (MQ=255)     gTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGt                                                                                                                                             <  1:430227/150‑1 (MQ=255)     gTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGt                                                                                                                                             >  1:955542/1‑150 (MQ=255)      tCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTg                                                                                                                                            >  2:1723796/1‑150 (MQ=255)        ggCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGTCACCGGTGATACCACCGGTgtc                                                                                                                                          <  1:2140629/150‑3 (MQ=255)        ggCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGTCACCGGTGATACCACCGGTgtc                                                                                                                                          <  2:2383937/150‑3 (MQ=255)        ggCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGAc                                                                                                                                          <  1:694837/150‑1 (MQ=255)           aaaTCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGaa                                                                                                                                       >  2:945986/1‑150 (MQ=255)             aTCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGTCACCGGTGATACCACCGGTGTCGatca                                                                                                                                     <  2:525541/150‑4 (MQ=255)              tCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTg                                                                                                                                    >  2:656952/1‑150 (MQ=255)                caccaTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTggg                                                                                                                                  <  2:31199/150‑1 (MQ=255)                 accaTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGc                                                                                                                                 <  1:945986/150‑1 (MQ=255)                 accaTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGGCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGc                                                                                                                                 >  1:987591/1‑150 (MQ=255)                 accaTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGAAATTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGc                                                                                                                                 >  1:1330734/1‑150 (MQ=255)                    aTGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATg                                                                                                                              <  1:1016787/150‑1 (MQ=255)                     tGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATgg                                                                                                                             <  1:1916064/150‑1 (MQ=255)                      gTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGa                                                                                                                            >  2:2034378/1‑150 (MQ=255)                            ccGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGaaaa                                                                                                                      >  1:263431/1‑150 (MQ=255)                              gCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGaaaaaa                                                                                                                    >  1:881586/1‑150 (MQ=255)                               ccTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAAt                                                                                                                   <  1:381864/150‑1 (MQ=255)                               ccTGACCAAGACCCGCGATGCAATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAAt                                                                                                                   <  2:553348/150‑1 (MQ=255)                                     cAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGa                                                                                                             >  1:632111/1‑150 (MQ=255)                                      aaGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAAt                                                                                                                   >  1:2184732/1‑143 (MQ=255)                                      aaGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAAt                                                                                                                   <  2:2184732/143‑1 (MQ=255)                                         aCCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGc                                                                                                                                 <  1:2008808/126‑1 (MQ=255)                                         aCCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGc                                                                                                                                 >  2:2008808/1‑126 (MQ=255)                                            cgcgATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCg                                                                                                      >  1:1193631/1‑150 (MQ=255)                                                   ccATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATgg                                                                                               >  2:1769640/1‑150 (MQ=255)                                                      tcgtcgGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTTGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCa                                                                                            >  2:1950349/1‑150 (MQ=255)                                                      tcgtcgGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGc                                                                                                                                 <  1:1708637/113‑1 (MQ=255)                                                      tcgtcgGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGc                                                                                                                                 >  2:1708637/1‑113 (MQ=255)                                                           ggTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGaa                                                                                                                                       <  1:2345064/102‑1 (MQ=255)                                                           ggTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGaa                                                                                                                                       >  2:2345064/1‑102 (MQ=255)                                                              gACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaagaa                                                                                    <  1:1035299/150‑1 (MQ=255)                                                                 cTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGcc                                                                                 >  2:483520/1‑150 (MQ=255)                                                                   gTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGa                                                                               >  1:485151/1‑150 (MQ=255)                                                                          cTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTa                                                                            >  2:154626/1‑146 (MQ=255)                                                                          cTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTa                                                                            <  1:154626/146‑1 (MQ=255)                                                                           tGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGc                                                                       <  1:2185286/150‑1 (MQ=255)                                                                           tGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGc                                                                       <  1:483520/150‑1 (MQ=255)                                                                            gACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCt                                                                      >  2:1445983/1‑150 (MQ=255)                                                                              cccGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGt                                                                    >  2:1979430/1‑150 (MQ=255)                                                                               ccGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATg                                                                                                                  <  1:2155322/103‑1 (MQ=255)                                                                               ccGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATg                                                                                                                  >  2:2155322/1‑103 (MQ=255)                                                                               ccGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTCTGTGCTGtt                                                                   >  1:695672/1‑150 (MQ=255)                                                                               ccGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGtt                                                                   >  2:1233161/1‑150 (MQ=255)                                                                                  tGACCGCCAGTATGGTGATGCCAGGTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCt                                                                >  2:1620812/1‑150 (MQ=255)                                                                                   gACCGCCAGTATGGGGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTAATCGACACCGGTGGTATCACCGGTGACGAAGTGAAAAAAAAAAAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTg                                                               <  2:541105/150‑1 (MQ=255)                                                                                     ccGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGt                                                             <  2:752445/150‑1 (MQ=255)                                                                                       gCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTg                                                           >  1:2051974/1‑150 (MQ=255)                                                                                       gCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCCCATGGCCATTgaa                                                                                       >  1:907741/1‑122 (MQ=255)                                                                                       gCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCCCATGGCCATTgaa                                                                                       <  2:907741/122‑1 (MQ=255)                                                                                           gTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGc                                                       >  2:2273240/1‑150 (MQ=255)                                                                                            tATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaa                                                                                       >  2:2298806/1‑117 (MQ=255)                                                                                            tATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTgaa                                                                                       <  1:2298806/117‑1 (MQ=255)                                                                                               gaccctGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGt                                                   >  2:1409690/6‑150 (MQ=255)                                                                                                accctGCCAGCTGGCAGGGTCGTTCCTTCATCCTGCTCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTACGTGCTGTTCCTGCTTGATGCCCGTg                                                  >  2:2166634/5‑150 (MQ=255)                                                                                                 tGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATAGCCACAGGCGGAGACACGGGAGCAGAAGTCGGCATGGACGAAAGACGGGGCGAGGAGTGGCGTATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGc                                                 >  2:270359/1‑150 (MQ=255)                                                                                                   aTGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCgg                                               <  1:1828826/150‑1 (MQ=255)                                                                                                      ccAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTa                                            <  2:263431/150‑1 (MQ=255)                                                                                                      ccAGCTGGCAGGGTCGTTCCTTCAGCCTGAGCGACACCGGTGGTAGCACCGGTGGCGAAGTGGGCATGGACGAAAAAAGGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTa                                            <  1:1097995/150‑1 (MQ=255)                                                                                                         gCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTTGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGa                                         <  1:1950349/150‑1 (MQ=255)                                                                                                             gCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGt                                                   <  1:1637398/136‑1 (MQ=255)                                                                                                             gCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGt                                                   >  2:1637398/1‑136 (MQ=255)                                                                                                               aGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCgg                                   <  2:485151/150‑1 (MQ=255)                                                                                                                   tCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGa                               >  1:419898/1‑150 (MQ=255)                                                                                                                    cGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGAc                              <  1:2143465/150‑1 (MQ=255)                                                                                                                       tCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAg                           >  1:2200140/1‑150 (MQ=255)                                                                                                                        ccTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                          <  2:632111/150‑1 (MQ=255)                                                                                                                        ccTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                          <  2:419898/150‑1 (MQ=255)                                                                                                                        ccTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCAGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                          <  1:1769640/150‑1 (MQ=255)                                                                                                                         cTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAgat                         >  2:2206167/1‑150 (MQ=255)                                                                                                                                 tGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCAGCAGACCAGATGATCGCCg                 >  2:2214630/1‑150 (MQ=255)                                                                                                                                 ggATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAATAATCGCCGCGCAGTCGCCCATGGCCATTGAAGAAGCCGACTATGTGCTGATCCTGGTGGATGCCGGTGCGGGTATGGCTGTGGCAGACGAGATTAACGCCg                 >  2:105048/2‑150 (MQ=255)                                                                                                                                   aTCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                          >  1:1411476/1‑139 (MQ=255)                                                                                                                                   aTCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAga                          <  2:1411476/139‑1 (MQ=255)                                                                                                                                   aTCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAg               >  2:872033/1‑150 (MQ=255)                                                                                                                                    tCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGc              <  1:2273240/150‑1 (MQ=255)                                                                                                                                      gACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCAc            >  2:1813148/1‑150 (MQ=255)                                                                                                                                       tcacCGGTGATACCACCGGTGTCGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCAcc           >  1:350791/2‑150 (MQ=255)                                                                                                                                       tcacCGGTGATACCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTAtg                                                                          <  2:1762338/86‑1 (MQ=255)                                                                                                                                       tcacCGGTGATACCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTAtg                                                                          >  1:1762338/2‑87 (MQ=255)                                                                                                                                        cacCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCt          <  1:656952/150‑1 (MQ=255)                                                                                                                                         gccGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTg         >  2:907771/2‑150 (MQ=255)                                                                                                                                          ccGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTgc        <  2:2051974/150‑1 (MQ=255)                                                                                                                                             gtggtATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTGCGCa     >  2:2312739/1‑150 (MQ=255)                                                                                                                                              tggtATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGa                                         <  1:1857981/113‑1 (MQ=255)                                                                                                                                              tggtATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGa                                         >  2:1857981/1‑113 (MQ=255)                                                                                                                                                gtATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGCATCGAGTCCAGGCGGGTATGACTGCGTCAGACCAGATGATCGCCGCCCCCCGACGCAAGc  >  2:1146170/1‑150 (MQ=255)                                                                                                                                                gtATCACCGGAGACGAAGTGGCCACGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTGCGCAAGc  >  2:1845273/1‑150 (MQ=255)                                                                                                                                                   tCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTAtgtg                                                                        <  1:219993/77‑1 (MQ=255)                                                                                                                                                   tCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTAtgtg                                                                        >  2:219993/1‑77 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GAACGTCGGCAAATCCACCATGTTCAACCGCCTGACCAAGACCCGCGATGCCATCGTCGGTGACCTGTCGGGCCTGACCCGTGACCGCCAGTATGGTGATGCCAGCTGGCAGGGTCGTTCCTTCATCCTGATCGACACCGGTGGTATCACCGGTGACGAAGTGGGCATGGACGAAAAAATGGCCGAGCAGTCGCTCATGGCCATTGAAGAAGCCGACTATGTGCTGTTCCTGGTTGATGCCCGTGCGGGTATGACTGCGGCAGACCAGATGATCGCCGAGCACCTGCGCAAGC  >  NC_002947/991414‑991706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑