Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,391,002 G→C 7.3% G268A (GGC→GCC)  aspS → aspartate‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,391,0020GC 7.3% 244.5 / 7.9 78G268A (GGC→GCC) aspSaspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (39/33);  new base C (4/2);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CTTCCGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCAACG  >  NC_002947/1390853‑1391145
                                                                                                                                                     |                                                                                                                                               
cTTCCGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACAGCAAGTATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCgg                                                                                                                                                 <  2:1534049/150‑1 (MQ=255)
        aCGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTcc                                                                                                                                         <  1:801943/150‑1 (MQ=255)
         cGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTccc                                                                                                                                        <  1:361420/150‑1 (MQ=255)
          gAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCg                                                                                                                                       <  1:1165151/150‑1 (MQ=255)
           aaGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGc                                                                                                                                      >  2:2095379/1‑150 (MQ=255)
             gACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCGGGAATTCGCCGAATTccagatc                                                                                                                                    >  2:187886/1‑145 (MQ=255)
                cTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATg                                                                                                                                 >  2:2095852/1‑150 (MQ=255)
                  gCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGAc                                                                                                                               >  1:905182/1‑150 (MQ=255)
                  gCGTGCCGACCGTCAGCCGGAATACACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTAGCATTCGCCGCATTCCCCAACATGAc                                                                                                                               >  2:475707/1‑150 (MQ=255)
                   cGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGAcc                                                                                                                              >  2:1725839/1‑150 (MQ=255)
                         gACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGaa                                                                                                                        <  2:978133/150‑1 (MQ=255)
                                 gCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGcc                                                                                                                  <  1:966166/148‑1 (MQ=255)
                                 gCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGcc                                                                                                                  >  2:966166/1‑148 (MQ=255)
                                 gCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCAt                                                                                                                >  1:960767/1‑150 (MQ=255)
                                 gCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCAt                                                                                                                <  1:2027155/150‑1 (MQ=255)
                                        ttCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGTGCCTGAGCGAAGGCATGATCGATGAGCTGATCAAGGAAGTGCTCGATCTCGAATTAGGCGCATTCCCGCACATGACCTTCGAAGAGGCCATGcgccgc                                                                                                         >  2:772276/1‑150 (MQ=255)
                                           aCCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGaa                                                                                                                        <  2:736057/132‑1 (MQ=255)
                                           aCCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGaa                                                                                                                        >  1:736057/1‑132 (MQ=255)
                                             ccAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACgg                                                                                                    >  1:2307882/1‑150 (MQ=255)
                                             ccAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACgg                                                                                                    >  1:774271/1‑150 (MQ=255)
                                                   cGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGa                                                                                              <  1:2294613/150‑1 (MQ=255)
                                                     aCATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGCCGAATTccaga                                                                                                                                      >  1:2245305/1‑105 (MQ=255)
                                                     aCATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGCCGAATTccaga                                                                                                                                      <  2:2245305/108‑4 (MQ=255)
                                                        tCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGc                                                                                         >  2:1996680/1‑150 (MQ=255)
                                                         cGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGcc                                                                                        >  1:1851499/1‑150 (MQ=255)
                                                             aCCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAagag                                                                                                                     <  2:2451613/117‑1 (MQ=255)
                                                             aCCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAagag                                                                                                                     >  1:2451613/1‑117 (MQ=255)
                                                             aCCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGAc                                                                                    >  2:322995/1‑150 (MQ=255)
                                                               cAGTTTCCTCGATGAAAGCGAGGTCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCGGGCGCCGCGACGGCTCCGACAAGCCAGACCt                                                                                  >  2:1946667/1‑150 (MQ=255)
                                                                aGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTg                                                                                 <  2:758278/150‑1 (MQ=255)
                                                                 gTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGc                                                                                <  2:905182/150‑1 (MQ=255)
                                                                  tttCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCg                                                                               >  2:2446271/1‑150 (MQ=255)
                                                                      cTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTAtt                                                                           >  1:1603904/1‑150 (MQ=255)
                                                                       tCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTc                                                                          >  1:2048378/1‑150 (MQ=255)
                                                                           tGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCt                                                                      <  2:1851499/150‑1 (MQ=255)
                                                                            gAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTg                                                                     >  2:752622/1‑150 (MQ=255)
                                                                             aaaGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTgg                                                                    >  1:425398/1‑150 (MQ=255)
                                                                               aGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGaa                                                                  <  2:960767/150‑1 (MQ=255)
                                                                               aGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGaa                                                                  >  1:212634/1‑150 (MQ=255)
                                                                                    gaTCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGt                                                             <  1:1177726/150‑1 (MQ=255)
                                                                                     aTCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGCCGAATTcc                                                                                                                                         <  1:2282847/73‑1 (MQ=255)
                                                                                     aTCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGCCGAATTcc                                                                                                                                         >  2:2282847/1‑73 (MQ=255)
                                                                                        aTGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGAc                                                         <  2:1603904/150‑1 (MQ=255)
                                                                                             ccTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGaa                                                                  >  2:717006/1‑136 (MQ=255)
                                                                                             ccTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGaa                                                                  <  1:717006/136‑1 (MQ=255)
                                                                                             ccTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGc                                                    >  2:2208094/1‑150 (MQ=255)
                                                                                              cTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGcc                                                   >  1:2101177/1‑150 (MQ=255)
                                                                                                 aCCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGAc                                                >  1:1277279/1‑150 (MQ=255)
                                                                                                 aCCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTATGCAGCCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGAc                                                <  1:1946667/150‑1 (MQ=255)
                                                                                                   cGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTg                                                               >  1:2044246/1‑133 (MQ=255)
                                                                                                   cGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTg                                                               <  2:2044246/133‑1 (MQ=255)
                                                                                                   cGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGATGTTGCCGACCa                                              <  1:322995/150‑1 (MQ=255)
                                                                                                    gAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAg                                             >  1:817380/1‑150 (MQ=255)
                                                                                                     aaaGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGcgcc                                                                                                           >  2:1507664/1‑87 (MQ=255)
                                                                                                     aaaGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGcgcc                                                                                                           <  1:1507664/87‑1 (MQ=255)
                                                                                                      aaGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCTCTGGAACTGGTCGACGTTGCCGACCAGCt                                           <  1:2095379/150‑1 (MQ=255)
                                                                                                          aTGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAg                                       <  1:2095852/150‑1 (MQ=255)
                                                                                                             aTCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGc                                                                                <  1:1070136/106‑1 (MQ=255)
                                                                                                             aTCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGc                                                                                >  2:1070136/1‑106 (MQ=255)
                                                                                                             aTCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGAt                                    <  2:2005280/150‑1 (MQ=255)
                                                                                                              cccGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGACACACCGCCCATGCACTCCAATGACGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATg                                   <  2:2101177/149‑1 (MQ=255)
                                                                                                                  cAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGa                               >  1:2321670/1‑150 (MQ=255)
                                                                                                                   aaGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTGCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGAc                              >  2:1286753/1‑150 (MQ=255)
                                                                                                                        gTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCaa                         <  2:1277279/150‑1 (MQ=255)
                                                                                                                         ttCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGACCACGCCATGCGCCGCTACGGCGCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                        >  1:886837/1‑150 (MQ=255)
                                                                                                                         ttCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                        <  1:752622/150‑1 (MQ=255)
                                                                                                                         ttCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                        <  2:1610206/150‑1 (MQ=255)
                                                                                                                          tCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAgg                       <  1:2446271/150‑1 (MQ=255)
                                                                                                                            aaGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGt                                                             <  1:685874/110‑1 (MQ=255)
                                                                                                                            aaGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGt                                                             >  2:685874/1‑110 (MQ=255)
                                                                                                                            aaGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGgtg                     >  2:1664253/1‑150 (MQ=255)
                                                                                                                               gAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTc                  >  2:588780/1‑150 (MQ=255)
                                                                                                                                      tCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggcc           >  1:2309148/1‑150 (MQ=255)
                                                                                                                                         aTCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggcccgg        <  2:425398/150‑1 (MQ=255)
                                                                                                                                          tCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggcccggc       >  2:649088/1‑150 (MQ=255)
                                                                                                                                            tGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCa     >  2:1491764/1‑150 (MQ=255)
                                                                                                                                             ggAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCaa    <  2:2048378/150‑1 (MQ=255)
                                                                                                                                              gAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCAAc   <  2:1772601/150‑1 (MQ=255)
                                                                                                                                               aaTTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCAGCCCGGCCAACg  >  2:129536/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CTTCCGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCAACG  >  NC_002947/1390853‑1391145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑