Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,475,003	C→A	19.7%	intergenic (‑776/+25)	algD ← / ← PP_1289	GDP‑mannose 6‑dehydrogenase/DUF328 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,475,003	0	C	A	19.7%	141.3 / 27.3	66	intergenic (‑776/+25)	algD/PP_1289	GDP‑mannose 6‑dehydrogenase/DUF328 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (22/31);  new base A (5/8);  total (27/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

TCAAAAAAGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGC  >  NC_002947/1474854‑1475142                                                                                                                                                      |                                                                                                                                            tCAAAAAAGGTATGAACGGTCTTATGACGTTTAGCGAGAACTGGCCGGTCGACGTGGTTTTGGCGTCGGGGTTGTGGCGAGATGGCTTTTTACAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCACTTCGCGGCGTc                                                                                                                                             >  1:2387362/1‑150 (MQ=255)   aaaaaaGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAg                                                                                                                                           <  2:1358627/150‑1 (MQ=255)   aaaaaaGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATGTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAg                                                                                                                                           >  1:780286/1‑150 (MQ=255)   aaaaaaGGTATGAACGGTCTTATGGCGTTTGGAGATACAGGGAGGTGCGACGCACTTTTGGCGCGAGAGTAGAGGTTACAGAGCGGTTTGCTGCTTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAg                                                                                                                                           >  2:866232/1‑150 (MQ=255)    aaaaaGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGc                                                                                                                                          >  1:96112/1‑150 (MQ=255)       aaGGTATGAGCGGTCTTATGGGGTTTGGCGATAAAGTGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGCCCTCTTCGCGGCTGAAGCcgc                                                                                                                                       <  2:1076952/150‑1 (MQ=255)          gTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGaa                                                                                                                                    >  2:1405729/1‑150 (MQ=255)          gTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGaa                                                                                                                                    <  1:907352/150‑1 (MQ=255)            aTGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAaga                                                                                                                                  <  1:2328095/150‑1 (MQ=255)             tGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAagag                                                                                                                                 <  1:1234898/150‑1 (MQ=255)             tGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAagag                                                                                                                                 <  2:282057/150‑1 (MQ=255)             tGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAagag                                                                                                                                 <  2:2228476/150‑1 (MQ=255)             ggAACGGTCTTATGGCGTTTGGCGATAATGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAagag                                                                                                                                 <  2:1726084/149‑1 (MQ=255)               aaCGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAggg                                                                                                                               >  2:1815119/1‑150 (MQ=255)                    tCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGa                                                                                                                          <  2:96112/150‑1 (MQ=255)                      ttATGGCGTTTCGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACa                                                                                                                        >  2:2094376/1‑150 (MQ=255)                        aTGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAgg                                                                                                                      <  1:1405729/150‑1 (MQ=255)                        aTGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAgg                                                                                                                      <  1:502455/150‑1 (MQ=255)                                tGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTcc                                                                                                              >  2:1397363/1‑150 (MQ=255)                                  gCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCcgc                                                                                                            >  1:1250548/1‑150 (MQ=255)                                   cGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAAGAgg                                                                                                                                <  1:1507713/129‑1 (MQ=255)                                   cGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAAGAgg                                                                                                                                >  2:1507713/1‑129 (MQ=255)                                    gATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCgg                                                                                                          <  1:1815119/150‑1 (MQ=255)                                     aTAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCggg                                                                                                         <  1:2094376/150‑1 (MQ=255)                                     aTAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCCCTCCGCGGCTTCAGCCGCGACGAGGGCGTGACAGGCTCACTCCGCggg                                                                                                         <  2:1969520/150‑1 (MQ=255)                                      tAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCgggg                                                                                                        <  2:196240/150‑1 (MQ=255)                                         aGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGaa                                                                                                                                    <  2:693317/119‑1 (MQ=255)                                         aGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGaa                                                                                                                                    >  1:693317/1‑119 (MQ=255)                                         aGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGa                                                                                                     <  2:2387362/150‑1 (MQ=255)                                          gggAGGTGCGACGGATTTCTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGAt                                                                                                    <  1:833446/150‑1 (MQ=255)                                           ggAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATc                                                                                                   <  1:1918185/150‑1 (MQ=255)                                                tGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGTCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCa                                                                                              <  2:2255956/150‑1 (MQ=255)                                                tGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCa                                                                                              <  2:2254264/150‑1 (MQ=255)                                                 gCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAg                                                                                             <  2:1351117/150‑1 (MQ=255)                                                       gATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAacac                                                                                       <  1:1397363/150‑1 (MQ=255)                                                        aTTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACAcc                                                                                      <  2:1492935/150‑1 (MQ=255)                                                                      aTTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGAGATAGACTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCtt                                                                        <  2:2169384/150‑1 (MQ=255)                                                                         cGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGa                                                                     <  2:1250548/150‑1 (MQ=255)                                                                          gTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGaa                                                                    >  1:2052616/1‑149 (MQ=255)                                                                          gTGGCGTGAGGGCGGTTTGCAGCGTGCCCTTTGGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCGTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGAc                                                                    <  2:39546/150‑1 (MQ=255)                                                                                  aTGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATc                                                                                                   >  2:93825/1‑111 (MQ=255)                                                                                  aTGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATc                                                                                                   <  1:93825/111‑1 (MQ=255)                                                                                     gCGGTTTGCAGCGTCCCCTGTTGCAGCACAGAGGCCAATACCGGAGGGTGACCCCGTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACt                                                         <  1:982061/150‑1 (MQ=255)                                                                                            gCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagtag                                                  >  1:2046024/1‑150 (MQ=255)                                                                                              aGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCggg                                                                                                         <  1:310762/93‑1 (MQ=255)                                                                                              aGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCggg                                                                                                         >  2:310762/1‑93 (MQ=255)                                                                                              aGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagtagta                                                >  1:1953664/1‑150 (MQ=255)                                                                                                 gTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTAccc                                             <  1:352127/150‑1 (MQ=255)                                                                                                   gCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGTGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCt                                           <  2:2233659/150‑1 (MQ=255)                                                                                                   gCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCt                                           >  2:1539821/1‑150 (MQ=255)                                                                                                     cctgcaacAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGa                                         <  2:88227/143‑1 (MQ=255)                                                                                                       tGTTGCAGGGGAGCGGCGACTCACGGCGGCTGACCTCTTCGCGGCCGCAGCCGCGGAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGc                                       >  2:279246/1‑150 (MQ=255)                                                                                                          tGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATc                                    <  1:1539821/150‑1 (MQ=255)                                                                                                                                    cTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGc          >  2:958451/1‑150 (MQ=255)                                                                                                                                       cccTCTTCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGTACTGCTTGAGCTGTTCCGGGGTGCTGa       >  2:1268091/2‑150 (MQ=255)                                                                                                                                       aCCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGa       <  2:2046024/150‑1 (MQ=255)                                                                                                                                        ccTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGAt      >  2:1002738/1‑150 (MQ=255)                                                                                                                                         ctctTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATg     >  2:30768/1‑150 (MQ=255)                                                                                                                                         ctctTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATg     >  2:31019/1‑150 (MQ=255)                                                                                                                                          tctTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATgc    >  1:2421519/1‑150 (MQ=255)                                                                                                                                           ctTCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCg                >  2:1216500/1‑137 (MQ=255)                                                                                                                                           ctTCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCg                <  1:1216500/137‑1 (MQ=255)                                                                                                                                            ttCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCggg              <  1:940373/138‑1 (MQ=255)                                                                                                                                            ttCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCggg              >  2:940373/1‑138 (MQ=255)                                                                                                                                            ttCGCGGCTGAAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATgcgc  >  1:1439436/1‑150 (MQ=255)                                                                                                                                                 ggCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGt            <  2:478922/135‑1 (MQ=255)                                                                                                                                                 ggCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGt            >  1:478922/1‑135 (MQ=255)                                                                                                                                                      |                                                                                                                                            TCAAAAAAGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGC  >  NC_002947/1474854‑1475142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑