| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,969,520 | G→C | 7.0% | L15V (CTG→GTG) | ubiG ← | ubiquinone biosynthesis O‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,969,520 | 0 | G | C | 7.0% | 172.9 / 4.9 | 57 | L15V (CTG→GTG) | ubiG | ubiquinone biosynthesis O‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (19/34); new base C (2/2); total (21/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
TTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGATATCCGAATGACGGGGAG > NC_002947/1969374‑1969667 | ttCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAgcgc > 1:2243296/1‑150 (MQ=255) gAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGaa < 2:1339402/150‑1 (MQ=255) gATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACtt < 2:2080355/150‑1 (MQ=255) aTGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTg < 2:254833/150‑1 (MQ=255) tGCCGCCACCGCAACCCCCGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCGTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTgg < 2:84685/150‑1 (MQ=255) tGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTgg < 2:343113/150‑1 (MQ=255) gccgccACCGCAACCACCGTGCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGCTACAGCAG‑CGCTGTGTCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGc < 1:1002525/150‑1 (MQ=255) gccACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGCTGGCCCACCGCtggtgg > 1:1078912/1‑136 (MQ=255) gccACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGCTGGCCCACCGCtggtgg < 2:1078912/141‑6 (MQ=255) ccGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCg < 2:2359842/150‑1 (MQ=255) cAACCCACGTCCAGCACTTTCTTCACAACCATGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGgcg < 1:1257/150‑1 (MQ=255) ccACGTCCAGCACTTTCTTTCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGt > 2:479466/1‑150 (MQ=255) ccACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGCTGGCCCACCGCtggtgg > 1:2428658/1‑125 (MQ=255) ccACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGCTGGCCCACCGCtggtgg < 2:2428658/130‑6 (MQ=255) cACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTc > 2:149620/1‑150 (MQ=255) gTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGAc < 1:988429/150‑1 (MQ=255) aGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTg < 1:1008765/150‑1 (MQ=255) aGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTg > 1:1191781/1‑150 (MQ=255) ttGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGaa < 1:1714976/150‑1 (MQ=255) tGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAAc < 2:1349096/150‑1 (MQ=255) ggCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTc < 1:2408688/150‑1 (MQ=255) ggCCAGGCTCACGCGCTCGTCGATCCAGTTGACGC‑GATCTGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTc < 1:1084518/150‑1 (MQ=255) ccAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCct < 2:2243296/150‑1 (MQ=255) cTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCCACGCCGTTTGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGaa > 1:1860921/1‑150 (MQ=255) tGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGaaa < 2:432247/150‑1 (MQ=255) gACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGaaa < 1:75431/149‑1 (MQ=255) gACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGaaa > 2:75431/1‑149 (MQ=255) gcgcTCGTCGATCCAGTTGACGC‑GCCGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTc < 2:1191781/150‑1 (MQ=255) gcgcTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTc < 1:803327/150‑1 (MQ=255) gcgcTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTc < 1:149620/150‑1 (MQ=255) gcTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACtt < 1:173330/97‑1 (MQ=255) gcTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACtt > 2:173330/1‑97 (MQ=255) tcgtcgATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCaa > 2:2341426/1‑150 (MQ=255) gtcgATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGAc > 1:1445251/1‑114 (MQ=255) gtcgATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGAc < 2:1445251/114‑1 (MQ=255) gtcgATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCaaaa < 1:2341426/150‑1 (MQ=255) cAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGcc < 2:2089864/150‑1 (MQ=255) cAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATAccc > 2:881998/1‑150 (MQ=255) cAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATAccc < 1:2312383/150‑1 (MQ=255) agttCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTgg < 2:367859/148‑1 (MQ=255) cAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGa > 2:446727/1‑124 (MQ=255) cAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGa < 1:446727/124‑1 (MQ=255) gCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGt > 1:218570/1‑150 (MQ=255) gAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCtata < 2:218570/150‑1 (MQ=255) cTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATc < 2:1492345/150‑1 (MQ=255) tCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCg > 1:1015189/1‑150 (MQ=255) tttCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTAGACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCgg > 2:1406188/1‑150 (MQ=255) gcgGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGtttt > 2:1670263/1‑150 (MQ=255) cgGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGttttt < 1:2030379/150‑1 (MQ=255) caccaGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGATCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGATATc > 1:2092199/1‑150 (MQ=255) accaGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGATATcc > 1:1458315/1‑150 (MQ=255) caGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGata < 1:2398782/145‑1 (MQ=255) caGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGata > 2:2398782/1‑145 (MQ=255) aGCGGTGGGCCCGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGATATCCGaa < 1:1566875/150‑1 (MQ=255) tGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGATATCCGAATGACg < 1:214448/150‑1 (MQ=255) gggCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGATATCCGAATGACgg > 2:1027785/1‑150 (MQ=255) ccAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGATATCCGAATGACGGGga < 2:1860921/150‑1 (MQ=255) cAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGATATCCGAATGACGGGgag > 1:616377/1‑150 (MQ=255) | TTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGC‑GCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTTGATATCCGAATGACGGGGAG > NC_002947/1969374‑1969667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |