Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,128,276 A→T 5.7% M1202L (ATG→TTG)  PP_1887 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,128,2760AT 5.7% 266.4 / 6.2 88M1202L (ATG→TTG) PP_1887hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (40/43);  new base T (2/3);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

GCGCCCGTGGACCCCTCCGGTCAAACCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATG  >  NC_002947/2128129‑2128420
                                                                                                                                                   |                                                                                                                                                
gcgcCCGTGGACCCCTCCGGTCAAACCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATg                                                                                                                                                >  2:1423033/1‑150 (MQ=255)
 cgcCCGTGGACGCCACCGGTGAAACCAGTGCCAGGCAGATATTCGGCTTCCGTGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGa                                                                                                                                               <  2:1134178/150‑1 (MQ=255)
 cgcCCGTGGACCCCTCCGGTCAAACCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGa                                                                                                                                               <  1:2345661/150‑1 (MQ=255)
     cGTGGACCTCTCCGGTCAACCCATTGCCAGGCAGATATTCGGTTTGGTGCGGCCGATCGCCGTCTTTACTGTTATCATCCACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCg                                                                                                                                           <  1:2112799/150‑1 (MQ=255)
     cGTGGACCCCTCCGGTCAAACCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCg                                                                                                                                           <  2:2172900/150‑1 (MQ=255)
        tgatcaggttgccattgccgtcgtaatgcaaatcgatagccaactgcggatcgtatttgacgatcttgaacaactcctccaggtcgtgatcgacgtcataggtctccagtagcgcatcaggcggaatGATCCGGCACAAATGAGCCGGAt                                                                                                                                        >  1:911781‑M1/128‑150 (MQ=255)
           ccccTCCGGTCAAACCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCAt                                                                                                                                     <  2:476794/150‑1 (MQ=255)
                 cGGTCAAACCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGcc                                                                                                                               >  2:560007/1‑150 (MQ=255)
                     cAAACCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGAt                                                                                                                           <  1:1423033/150‑1 (MQ=255)
                      aaaCCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGa                                                                                                                                         >  1:1411344/1‑135 (MQ=255)
                      aaaCCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGa                                                                                                                                         <  2:1411344/135‑1 (MQ=255)
                      aaaCCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCt                                                                                                                              <  2:971219/146‑1 (MQ=255)
                      aaaCCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCt                                                                                                                              >  1:971219/1‑146 (MQ=255)
                      aaaCCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATg                                                                                                                          >  2:1712616/1‑150 (MQ=255)
                      aaaCCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATAATTCCGCCTGATg                                                                                                                          >  2:1712169/1‑150 (MQ=255)
                       aaCCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACaaa                                                                                                                                                  >  1:956751/1‑125 (MQ=255)
                       aaCCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACaaa                                                                                                                                                  <  2:956751/125‑1 (MQ=255)
                        aCCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATgcg                                                                                                                        >  2:1552732/1‑150 (MQ=255)
                         ccATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATgcgc                                                                                                                       <  1:179283/150‑1 (MQ=255)
                         ccATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATgcgc                                                                                                                       <  1:2262773/150‑1 (MQ=255)
                         ccATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGCCACAAATGAGCCGGATCATTCCGCCTGATgcgc                                                                                                                       >  2:564236/1‑150 (MQ=255)
                               ccAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTgg                                                                                                                 <  1:1972316/150‑1 (MQ=255)
                                cAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGACCCCGATCATTCCGCCTGATGCGCTACTGga                                                                                                                <  1:665620/150‑1 (MQ=255)
                                  ggCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGgaga                                                                                                              <  1:689743/150‑1 (MQ=255)
                                   gCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGAc                                                                                                             >  1:2117284/1‑150 (MQ=255)
                                   gCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGAc                                                                                                             <  2:2239225/150‑1 (MQ=255)
                                    cAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGc                                                                                                                                             <  2:2021622/117‑1 (MQ=255)
                                    cAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGc                                                                                                                                             >  1:2021622/1‑117 (MQ=255)
                                       atatTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTAt                                                                                                         >  2:1180087/1‑150 (MQ=255)
                                       atatTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTAt                                                                                                         <  1:1552732/150‑1 (MQ=255)
                                             ggCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTc                                                                                                   >  1:1786007/1‑150 (MQ=255)
                                                ttCGATGCCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGAt                                                                                                <  2:1703755/150‑1 (MQ=255)
                                                         cTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGTCACGAGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTg                                                                                       >  1:1332538/1‑150 (MQ=255)
                                                         cTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTg                                                                                       >  2:370450/1‑150 (MQ=255)
                                                            gACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTggag                                                                                    >  1:2228159/1‑150 (MQ=255)
                                                            gACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTggag                                                                                    <  2:1468033/150‑1 (MQ=255)
                                                                  aTCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGa                                                                                                                            >  2:2236818/1‑104 (MQ=255)
                                                                  aTCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGa                                                                                                                            <  1:2236818/104‑1 (MQ=255)
                                                                  aTCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAgttg                                                                              >  2:1965937/1‑150 (MQ=255)
                                                                  aTCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAgttg                                                                              <  1:15463/150‑1 (MQ=255)
                                                                   tCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTgg                                                                                      >  1:1605470/1‑141 (MQ=255)
                                                                   tCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTgg                                                                                      <  2:1605470/141‑1 (MQ=255)
                                                                          ggTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGAt                                                                      >  1:2106689/1‑150 (MQ=255)
                                                                             gATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGcc                                                                                                                                            <  2:2214632/77‑1 (MQ=255)
                                                                             gATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGcc                                                                                                                                            >  1:2214632/1‑77 (MQ=255)
                                                                              aTCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTc                                                                  <  2:1420785/150‑1 (MQ=255)
                                                                               tCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCa                                                                 >  2:859795/1‑150 (MQ=255)
                                                                                 aCCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCaaa                                                               >  1:406522/1‑150 (MQ=255)
                                                                                 aCCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCaaa                                                               <  1:370450/150‑1 (MQ=255)
                                                                                  ccTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAAt                                                              >  2:887320/1‑150 (MQ=255)
                                                                                       aGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGAt                                                         <  2:2117284/150‑1 (MQ=255)
                                                                                       aGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATACCGCAGTGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGAt                                                         >  1:61592/1‑150 (MQ=255)
                                                                                         aCCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTAt                                                                                                         >  2:275405/1‑100 (MQ=255)
                                                                                         aCCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTAt                                                                                                         <  1:275405/100‑1 (MQ=255)
                                                                                                    cGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTa                                            <  2:1200298/150‑1 (MQ=255)
                                                                                                    cGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTa                                            <  2:1199734/150‑1 (MQ=255)
                                                                                                     gTGGCAGCGGGCCTTGTACGAATTCAAGAACAGCTATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGCCCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTAt                                           <  2:602862/150‑1 (MQ=255)
                                                                                                     gTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTAt                                           >  2:2139456/1‑150 (MQ=255)
                                                                                                      tGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATc                                          <  1:1712616/150‑1 (MQ=255)
                                                                                                      tGGCAGCGGACCTTGTACGAATGCAAGAAGAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATc                                          <  1:1712169/150‑1 (MQ=255)
                                                                                                       ggCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCg                                         >  2:221680/1‑150 (MQ=255)
                                                                                                             ggACCATGTAGGAATTCAGGAATAGCGATCCGGCACAAATGGGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGc                                   <  2:194805/150‑1 (MQ=255)
                                                                                                               aCCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCAt                                 >  2:2158772/1‑150 (MQ=255)
                                                                                                               aCCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCAt                                 <  1:560007/150‑1 (MQ=255)
                                                                                                                 cTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTa                               >  2:2271531/1‑150 (MQ=255)
                                                                                                                 cTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTa                               >  1:2211554/1‑150 (MQ=255)
                                                                                                                     tACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTc                                                                           <  1:1284565/102‑1 (MQ=255)
                                                                                                                     tACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTc                                                                           >  2:1284565/1‑102 (MQ=255)
                                                                                                                         gcatcaggcggaatGATCCGGCTCATTTGTGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTc                                                                  >  1:557136‑M1/15‑107 (MQ=255)
                                                                                                                         gcatcaggcggaatGATCCGGCTCATTTGTGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTc                                                                  <  2:557136‑M1/93‑1 (MQ=255)
                                                                                                                           ttCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAAt                     <  1:859795/150‑1 (MQ=255)
                                                                                                                            tCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATATGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATg                    <  1:1092427/150‑1 (MQ=255)
                                                                                                                            tCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATg                    <  2:1332538/150‑1 (MQ=255)
                                                                                                                              aaGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGc                  >  1:712756/1‑150 (MQ=255)
                                                                                                                               aGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCa                 >  1:695138/1‑150 (MQ=255)
                                                                                                                                 cggnatGATCCGGCTCATTTGTGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAAc               <  2:1509660‑M1/144‑1 (MQ=255)
                                                                                                                                 aaTAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAAc               <  2:2106689/150‑1 (MQ=255)
                                                                                                                                  ggaatGATCCGGCTCATTTGTGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAgttgt                                                                             >  1:26645‑M1/6‑87 (MQ=255)
                                                                                                                                  ggaatGATCCGGCTCATTTGTGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAgttgt                                                                             <  2:26645‑M1/82‑1 (MQ=255)
                                                                                                                                   tAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCt             >  1:1174558/1‑150 (MQ=255)
                                                                                                                                   tAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTAATGGGAACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCt             <  1:146696/150‑1 (MQ=255)
                                                                                                                                     gCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGa           <  2:695138/150‑1 (MQ=255)
                                                                                                                                       GATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATc         >  2:712756‑M1/1‑150 (MQ=255)
                                                                                                                                        ATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCa        >  1:1720101‑M1/1‑150 (MQ=255)
                                                                                                                                         TCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAg       >  1:794191‑M1/1‑150 (MQ=255)
                                                                                                                                           CGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGtg     <  1:1345957‑M1/150‑1 (MQ=255)
                                                                                                                                             GCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGtgat   <  1:339815‑M1/150‑1 (MQ=255)
                                                                                                                                              CACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGAttt                                     <  2:2397772‑M1/115‑1 (MQ=255)
                                                                                                                                              CACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGAttt                                     >  1:2397772‑M1/1‑115 (MQ=255)
                                                                                                                                              CACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGtgatg  >  2:2228159‑M1/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCGCCCGTGGACCCCTCCGGTCAAACCATTGCCAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATG  >  NC_002947/2128129‑2128420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑