| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 205,123 | C→T | 6.6% | T3543T (ACC→ACT) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 205,123 | 0 | C | T | 6.6% | 168.4 / 2.6 | 60 | T3543T (ACC→ACT) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (25/31); new base T (2/2); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
GATCAATAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGCCTGGTA > NC_002947/205033‑205271 | tagcagtgcccacggtgccgttggccggcacggtgatgacggttttgccgtcactcagcgtgaaggtcaatgcactGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCt > 1:41213‑M1/77‑133 (MQ=255)tagcagtgcccacggtgccgttggccggcacggtgatgacggttttgccgtcactcagcgtgaaggtcaatgcactGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCt < 2:41213‑M1/57‑1 (MQ=255) cccacggtgccattagccggcacggtgatgacggttttgccgtcgctcagcgtgaaggtcaatgcactGTGGGTACTGCCACCGTGACTGCCCCGGACAACGTCTACGTTGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTg < 2:1587679‑M1/82‑1 (MQ=255) ccacggtgccgttggccggcacggtgatgacggttttgccgtcgctcagcgtgaaggtcaatgcactGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGa < 1:1323662‑M1/83‑1 (MQ=255) ccacggtgccattagccggcacggtgatgacggttttgccgtcgctcagcgtgaaggtcaatgcactGTGGGTACTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGa < 1:829079‑M1/83‑1 (MQ=255) acaaggtgccgttggtcggcacggtgatgacggttttgccgtcgctcagcgtgaaggtcaatgcactGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGa < 2:874514‑M1/83‑1 (MQ=255) acggtgccgttggccggcacggtgatgacggttttgccgtcactcagcgtgaaggtcaatgcactGTGGGCAATGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCgttggag < 1:885656‑M1/85‑4 (MQ=255) gttggccggcacggtgatgacggttttgccgtcgctcagcgtgaaggtcaacggcaccGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGa < 2:968788‑M1/92‑1 (MQ=255) gACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGt < 2:727342/150‑1 (MQ=32) ggccggcaaggttataacggttttgccgtcgctcagcgtgaaggtcaatgcactGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTCCCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTc > 2:1323662‑M1/55‑150 (MQ=255) gccggcacggtgatgacggttttgccgtcgctcagcgtgaaggtcaatgcactGTGGGTACTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAatcgat > 2:1359742‑M1/54‑124 (MQ=255) gccggcacggtgatgacggttttgccgtcgctcagcgtgaaggtcaatgcactGTGGGTACTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAatcgat < 1:1359742‑M1/71‑1 (MQ=255) ccTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCg > 2:968181/1‑150 (MQ=32) cTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCTGTACAACGTCTACGTCGGTACCACAGCCGTTCAGGGCAAATCTATTGCAACCGTTGAAGGTGCGGATGTCgg > 1:2376551/1‑150 (MQ=11) cTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCgg > 2:1292624/1‑150 (MQ=32) ttCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGc > 2:1792039/1‑150 (MQ=33) acggtgatgacggttttgccgtcgctcagcgtgccggccaacggcaccGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCaa < 1:158249‑M1/50‑1 (MQ=255) acggtgatgacggttttgccgtcgctcagcgtgccggccaacggcaccGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCaa > 2:158249‑M1/49‑98 (MQ=255) tgatgacggttttgccgtcgctcagcgtgaaggtcaatgcactGTGGGTACTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGa > 2:829079‑M1/44‑150 (MQ=255) gatgacggttttgccgtcgctcagcgtgaaggtcaatgcactGTGGGTACTGCCACCGTGACTGCCCCGGACAACGTCTACGTTGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCgag > 1:1587679‑M1/43‑149 (MQ=255) aGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGaa < 2:2404324/150‑1 (MQ=37) aGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGaa < 2:2194531/150‑1 (MQ=37) aGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGaa < 1:732385/150‑1 (MQ=37) aCGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGaacaac < 2:944418/150‑1 (MQ=39) aCGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGaacaac < 1:1326557/150‑1 (MQ=39) aCGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGCTCGaacaac < 2:1107487/150‑1 (MQ=38) cGGCAAAACCGGCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGACCAAATCGATCGCAACCGTGGAAGGTGCGGATGTCGGCAAGTTCGAACAACt < 1:273344/150‑1 (MQ=255) ggCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTg > 1:762261/1‑150 (MQ=38) gCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCTGAGAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGa < 2:72348/150‑1 (MQ=255) gCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGa > 1:1467116/1‑150 (MQ=38) gCAAAACCGTCATCACCGTGCCGGACCCCATCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGa > 1:1242977/1‑150 (MQ=14) aaaaCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGaca > 1:429470/1‑150 (MQ=38) gTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGAc < 1:968181/150‑1 (MQ=255) gTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGAc > 1:1567180/1‑150 (MQ=255) catcaCCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACaa < 2:2234638/150‑1 (MQ=255) atcaCCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAg > 1:1395005/1‑150 (MQ=255) atcaCCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAg > 2:2386942/1‑150 (MQ=255) tcaCCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGa > 1:873128/1‑150 (MQ=255) tcaCCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGa < 2:243763/150‑1 (MQ=255) gTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCg < 2:1163034/150‑1 (MQ=255) cgttggccGGCACGGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCa < 1:190218/143‑1 (MQ=255) cGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCa > 2:1792117/1‑150 (MQ=255) ggCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAg > 1:1256378/1‑150 (MQ=255) gCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGAcca > 2:1240006/1‑135 (MQ=255) gCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACaa < 1:1240006/137‑1 (MQ=255) gCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACaa > 1:2293625/1‑137 (MQ=255) gCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACaa < 2:2293625/137‑1 (MQ=255) cAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCAc > 2:1777307/1‑150 (MQ=255) ggCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCg < 1:1290162/103‑1 (MQ=33) ggCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCg > 2:1290162/1‑103 (MQ=32) ggCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTAcc < 2:2421267/150‑1 (MQ=255) ccGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCa < 2:429470/150‑1 (MQ=255) GTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCAcc < 1:685621‑M1/150‑1 (MQ=255) cACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACcgacga < 2:1411402/150‑1 (MQ=255) tGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGcc < 1:608168/150‑1 (MQ=255) tGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGcc > 2:705075/1‑150 (MQ=255) cAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGCCTg < 2:2114750/150‑1 (MQ=255) aaCCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGCCTgg > 2:1839169/1‑150 (MQ=255) aaCCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGCCTgg < 1:1839169/150‑1 (MQ=255) aCCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACCAACGAGCCGGGt > 2:1358013/1‑150 (MQ=255) ccGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGCCTGGTa > 2:1654872/1‑150 (MQ=255) | GATCAATAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAACAACTGACACTCGACAAGACCCCGGTCAGCACTACCGTCACCGACGAGCCTGGTA > NC_002947/205033‑205271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |