Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,404,130 G→C 13.3% A238A (GCG→GCC)  PP_2106 → ammonium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,404,1300GC13.3% 204.0 / 21.5 75A238A (GCG→GCC) PP_2106ammonium transporter
Reads supporting (aligned to +/- strand):  ref base G (38/27);  new base C (4/6);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

CGTGGGCGGTACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGC  >  NC_002947/2403981‑2404279
                                                                                                                                                     |                                                                                                                                                     
cGTGGGCGGTACCGCGATGGGCGGTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACTTAGC‑GGGCGGCAGCGGGCTGGTAGCgg                                                                                                                                                      >  2:1193901/1‑149 (MQ=255)
        gTACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTc                                                                                                                                               <  2:2047293/150‑1 (MQ=255)
         tACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCg                                                                                                                                              <  1:1493977/150‑1 (MQ=255)
          aCCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTTAGCGGCCTGGTGGCGATCAACTCGt                                                                                                                                             <  1:440726/150‑1 (MQ=255)
             gcgATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGa                                                                                                                                          <  2:1386396/150‑1 (MQ=255)
             gcgATGGGCGATTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATACTGATCATCGGCTGGTTCGGCTTCAACGTCATGACTGCCGAAACCGTGCCGTACATCAGCGGCCGGGTGGCGATCAACCCGtaaa                                                                                                                                          >  2:500539/1‑147 (MQ=255)
                aTGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATgg                                                                                                                                       >  2:1630786/1‑150 (MQ=255)
                   ggCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCa                                                                                                                                    <  2:805833/150‑1 (MQ=255)
                    gCGTTTGGTGGCTTTTGCGCCATCGGGTATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCAt                                                                                                                                   >  1:64593/1‑150 (MQ=255)
                     cGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCacc                                                                                                                                  <  2:2249578/150‑3 (MQ=255)
                         tggtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCAtggtgg                                                                                                                              <  1:386893/150‑1 (MQ=255)
                         tgctggCTTTTGCGCCATCGAGCATTCCCTTTCTCGCGCTGGGCTCGTGGATCCTCATCATCTGCTGCTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGCGCGTCAGCGGCCTTGTGGCGATCAACTCGTTGATGGCCAtggtgg                                                                                                                              <  2:450446/147‑1 (MQ=255)
                           gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGGGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                            <  2:799217/150‑1 (MQ=255)
                           gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                            <  1:650302/150‑1 (MQ=255)
                           gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGAGCCTCATCATCGGCTGGTTCGGCTTCAACGTTATGAGTGCCCAGACCTTGCAGTGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                            <  2:1230054/150‑1 (MQ=255)
                           gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGAGCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                            <  2:64593/150‑1 (MQ=255)
                              gCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGc                                                                                                                         >  1:935013/1‑150 (MQ=255)
                                ttttGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCAc                                                                                                                       >  2:904624/1‑150 (MQ=255)
                                   tGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCt                                                                                                                    >  1:31942/1‑150 (MQ=255)
                                      gcCATCGAGCATTCCCTTTCTGGCGCTGCGCTCGTGGATCCTCATCATCGGCTGGTTCGTCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTAATGCGCATGGTCGCGTGCACCCTGTc                                                                                                                 >  2:226602/1‑150 (MQ=255)
                                        cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAg                                                                                                               >  1:578593/1‑150 (MQ=255)
                                        cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCac                                                                                                                                   >  1:221543/1‑129 (MQ=255)
                                        cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCac                                                                                                                                   <  2:221543/130‑2 (MQ=255)
                                          tCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGAtc                                                                                                                                        <  2:611684/123‑2 (MQ=255)
                                          tCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGAtc                                                                                                                                        >  1:611684/1‑122 (MQ=255)
                                             aGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTg                                                                                                          >  1:797731/1‑150 (MQ=255)
                                                aTTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGGCCAGACCTTGCAGGGCGTCAGCGGCCTTGTGGCGATCAACTCGTTGATGTGCATGGTGGGTGGCACCCTGTAAGCGTTGCTg                                                                                                       >  1:144172/1‑150 (MQ=255)
                                                aTTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTg                                                                                                       <  1:1183646/150‑1 (MQ=255)
                                                 ttCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggtg                                                                                                                           <  1:1849519/129‑1 (MQ=255)
                                                 ttCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggtg                                                                                                                           >  2:1849519/1‑129 (MQ=255)
                                                  tCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGc                                                                                                     <  1:904624/150‑1 (MQ=255)
                                                   cccTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGcc                                                                                                    >  2:1695317/1‑150 (MQ=255)
                                                    ccTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCg                                                                                                   >  2:1021867/1‑150 (MQ=255)
                                                     cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                  >  1:1460381/1‑150 (MQ=255)
                                                     cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                  >  1:2054350/1‑150 (MQ=255)
                                                     cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                  >  2:199036/1‑150 (MQ=255)
                                                          tGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAAcgagt                                                                                                                                             <  1:2284390/102‑5 (MQ=255)
                                                          tGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAAcgagt                                                                                                                                             >  2:2284390/1‑98 (MQ=255)
                                                                          gATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCt                                                                             <  2:935013/150‑1 (MQ=255)
                                                                          gATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCt                                                                             <  1:1630786/150‑1 (MQ=255)
                                                                                catcatCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGa                                                                                         >  2:998138/1‑132 (MQ=255)
                                                                                catcatCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGa                                                                                         <  1:998138/132‑1 (MQ=255)
                                                                                  tcatcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCaccag                                                                                                                                <  1:1642270/91‑5 (MQ=37)
                                                                                  tcatcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCaccag                                                                                                                                >  2:1642270/1‑87 (MQ=37)
                                                                                     tcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGcc                                                                  >  2:755895/1‑150 (MQ=255)
                                                                                        gCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGc                                                               >  2:1052881/1‑150 (MQ=255)
                                                                                        gCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGc                                                               >  1:1500429/1‑150 (MQ=255)
                                                                                                 gCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGc                                                      >  2:549063/1‑150 (MQ=255)
                                                                                                 gCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGc                                                      >  1:1934545/1‑150 (MQ=255)
                                                                                                     cAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtggt                                                  <  2:1460381/150‑1 (MQ=255)
                                                                                                     cAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtggt                                                  <  2:578593/150‑1 (MQ=255)
                                                                                                     cAACGTCATGAGTGCCCAGACCTTGCAGGGCGTAAGTGGCCTGGTGGGGTTCAACTCGTTTTTGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtggt                                                  <  2:144172/150‑1 (MQ=255)
                                                                                                         gTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCg                                                                <  1:2172440/132‑1 (MQ=255)
                                                                                                         gTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCg                                                                >  2:2172440/1‑132 (MQ=255)
                                                                                                             tGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttt                                          >  1:1977459/1‑150 (MQ=255)
                                                                                                                gTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTgcg                                       >  1:1156678/1‑150 (MQ=255)
                                                                                                                gTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTgcg                                       <  2:2054350/150‑1 (MQ=255)
                                                                                                                   cccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAg                                    >  1:246374/1‑150 (MQ=255)
                                                                                                                   cccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAg                                    >  2:89018/1‑150 (MQ=255)
                                                                                                                   cccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAg                                    >  1:246403/1‑150 (MQ=255)
                                                                                                                    ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGc                                                                           >  2:834007/1‑110 (MQ=255)
                                                                                                                    ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGc                                                                           <  1:834007/110‑1 (MQ=255)
                                                                                                                    ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGAGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCCGATTTTCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttttgcgcgg                                   >  1:1984149/1‑143 (MQ=255)
                                                                                                                     cAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGt                                  >  2:1577887/1‑150 (MQ=255)
                                                                                                                     cAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAAAGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGt                                  >  2:1578738/1‑150 (MQ=255)
                                                                                                                          cTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGa                             <  1:89018/150‑1 (MQ=255)
                                                                                                                          cTTGCAGGGCGTCAGCGGCCTGGTGGCGAGCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGa                             >  1:1640315/1‑150 (MQ=255)
                                                                                                                                 ggCGTCAGCGGCCTGGTGGCGATCAACTCGTTGACGGCGATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATg                      <  1:500539/150‑1 (MQ=255)
                                                                                                                                   cGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGAGGCGATTTGCGCAGGTTCCGATCTGATGCa                    >  2:1279426/1‑150 (MQ=255)
                                                                                                                                       aGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCggg                                                       >  2:529451/1‑111 (MQ=255)
                                                                                                                                       aGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCggg                                                       <  1:529451/111‑1 (MQ=255)
                                                                                                                                             cTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggt          <  1:1577887/150‑1 (MQ=255)
                                                                                                                                             cTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAAAGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggt          <  1:1578738/150‑1 (MQ=255)
                                                                                                                                              tggtggCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggtg         >  2:902354/1‑150 (MQ=255)
                                                                                                                                              tggtggCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTGTCTGCATGACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggtg         >  1:1738712/1‑150 (MQ=255)
                                                                                                                                                   gCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTc    >  2:1481051/1‑150 (MQ=255)
                                                                                                                                                     gATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGc  >  2:994949/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTGGGCGGTACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGC  >  NC_002947/2403981‑2404279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑