Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,404,145 G→C 9.0% M243I (ATG→ATC)  PP_2106 → ammonium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,404,1450GC 9.0% 230.9 / 11.0 77M243I (ATG→ATC) PP_2106ammonium transporter
Reads supporting (aligned to +/- strand):  ref base G (43/27);  new base C (3/4);  total (46/31)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACCGGCCTGGT  >  NC_002947/2403997‑2404291
                                                                                                                                                    |                                                                                                                                                  
aTGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATgg                                                                                                                                                   >  2:1630786/1‑150 (MQ=255)
   ggCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCa                                                                                                                                                <  2:805833/150‑1 (MQ=255)
    gCGTTTGGTGGCTTTTGCGCCATCGGGTATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCAt                                                                                                                                               >  1:64593/1‑150 (MQ=255)
     cGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCacc                                                                                                                                              <  2:2249578/150‑3 (MQ=255)
         tggtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCAtggtgg                                                                                                                                          <  1:386893/150‑1 (MQ=255)
         tgctggCTTTTGCGCCATCGAGCATTCCCTTTCTCGCGCTGGGCTCGTGGATCCTCATCATCTGCTGCTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGCGCGTCAGCGGCCTTGTGGCGATCAACTCGTTGATGGCCAtggtgg                                                                                                                                          <  2:450446/147‑1 (MQ=255)
           gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGGGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                                        <  2:799217/150‑1 (MQ=255)
           gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                                        <  1:650302/150‑1 (MQ=255)
           gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGAGCCTCATCATCGGCTGGTTCGGCTTCAACGTTATGAGTGCCCAGACCTTGCAGTGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                                        <  2:1230054/150‑1 (MQ=255)
           gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGAGCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                                        <  2:64593/150‑1 (MQ=255)
              gCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGc                                                                                                                                     >  1:935013/1‑150 (MQ=255)
                ttttGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCAc                                                                                                                                   >  2:904624/1‑150 (MQ=255)
                   tGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCt                                                                                                                                >  1:31942/1‑150 (MQ=255)
                      gcCATCGAGCATTCCCTTTCTGGCGCTGCGCTCGTGGATCCTCATCATCGGCTGGTTCGTCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTAATGCGCATGGTCGCGTGCACCCTGTc                                                                                                                             >  2:226602/1‑150 (MQ=255)
                        cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAg                                                                                                                           >  1:578593/1‑150 (MQ=255)
                        cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCac                                                                                                                                               <  2:221543/130‑2 (MQ=255)
                        cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCac                                                                                                                                               >  1:221543/1‑129 (MQ=255)
                             aGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTg                                                                                                                      >  1:797731/1‑150 (MQ=255)
                                aTTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGGCCAGACCTTGCAGGGCGTCAGCGGCCTTGTGGCGATCAACTCGTTGATGTGCATGGTGGGTGGCACCCTGTAAGCGTTGCTg                                                                                                                   >  1:144172/1‑150 (MQ=255)
                                aTTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTg                                                                                                                   <  1:1183646/150‑1 (MQ=255)
                                 ttCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggtg                                                                                                                                       >  2:1849519/1‑129 (MQ=255)
                                 ttCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggtg                                                                                                                                       <  1:1849519/129‑1 (MQ=255)
                                  tCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGc                                                                                                                 <  1:904624/150‑1 (MQ=255)
                                   cccTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGcc                                                                                                                >  2:1695317/1‑150 (MQ=255)
                                    ccTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCg                                                                                                               >  2:1021867/1‑150 (MQ=255)
                                     cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                              >  1:2054350/1‑150 (MQ=255)
                                     cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                              >  2:199036/1‑150 (MQ=255)
                                     cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                              >  1:1460381/1‑150 (MQ=255)
                                                          gATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCt                                                                                         <  1:1630786/150‑1 (MQ=255)
                                                          gATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCt                                                                                         <  2:935013/150‑1 (MQ=255)
                                                                catcatCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGa                                                                                                     <  1:998138/132‑1 (MQ=255)
                                                                catcatCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGa                                                                                                     >  2:998138/1‑132 (MQ=255)
                                                                  tcatcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCaccag                                                                                                                                            >  2:1642270/1‑87 (MQ=37)
                                                                  tcatcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCaccag                                                                                                                                            <  1:1642270/91‑5 (MQ=37)
                                                                     tcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGcc                                                                              >  2:755895/1‑150 (MQ=255)
                                                                        gCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGc                                                                           >  1:1500429/1‑150 (MQ=255)
                                                                        gCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGc                                                                           >  2:1052881/1‑150 (MQ=255)
                                                                                 gCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGc                                                                  >  1:1934545/1‑150 (MQ=255)
                                                                                 gCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGc                                                                  >  2:549063/1‑150 (MQ=255)
                                                                                     cAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtggt                                                              <  2:578593/150‑1 (MQ=255)
                                                                                     cAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtggt                                                              <  2:1460381/150‑1 (MQ=255)
                                                                                     cAACGTCATGAGTGCCCAGACCTTGCAGGGCGTAAGTGGCCTGGTGGGGTTCAACTCGTTTTTGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtggt                                                              <  2:144172/150‑1 (MQ=255)
                                                                                         gTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCg                                                                            <  1:2172440/132‑1 (MQ=255)
                                                                                         gTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCg                                                                            >  2:2172440/1‑132 (MQ=255)
                                                                                             tGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttt                                                      >  1:1977459/1‑150 (MQ=255)
                                                                                                gTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTgcg                                                   <  2:2054350/150‑1 (MQ=255)
                                                                                                gTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTgcg                                                   >  1:1156678/1‑150 (MQ=255)
                                                                                                   cccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAg                                                >  1:246374/1‑150 (MQ=255)
                                                                                                   cccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAg                                                >  1:246403/1‑150 (MQ=255)
                                                                                                   cccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAg                                                >  2:89018/1‑150 (MQ=255)
                                                                                                    ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGc                                                                                       >  2:834007/1‑110 (MQ=255)
                                                                                                    ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGc                                                                                       <  1:834007/110‑1 (MQ=255)
                                                                                                    ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGAGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCCGATTTTCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttttgcgcgg                                               >  1:1984149/1‑143 (MQ=255)
                                                                                                     cAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGt                                              >  2:1577887/1‑150 (MQ=255)
                                                                                                     cAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAAAGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGt                                              >  2:1578738/1‑150 (MQ=255)
                                                                                                          cTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGa                                         <  1:89018/150‑1 (MQ=255)
                                                                                                          cTTGCAGGGCGTCAGCGGCCTGGTGGCGAGCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGa                                         >  1:1640315/1‑150 (MQ=255)
                                                                                                                 ggCGTCAGCGGCCTGGTGGCGATCAACTCGTTGACGGCGATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATg                                  <  1:500539/150‑1 (MQ=255)
                                                                                                                   cGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGAGGCGATTTGCGCAGGTTCCGATCTGATGCa                                >  2:1279426/1‑150 (MQ=255)
                                                                                                                       aGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCggg                                                                   <  1:529451/111‑1 (MQ=255)
                                                                                                                       aGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCggg                                                                   >  2:529451/1‑111 (MQ=255)
                                                                                                                             cTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggt                      <  1:1577887/150‑1 (MQ=255)
                                                                                                                             cTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAAAGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggt                      <  1:1578738/150‑1 (MQ=255)
                                                                                                                              tggtggCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggtg                     >  2:902354/1‑150 (MQ=255)
                                                                                                                              tggtggCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTGTCTGCATGACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggtg                     >  1:1738712/1‑150 (MQ=255)
                                                                                                                                   gCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTc                >  2:1481051/1‑150 (MQ=255)
                                                                                                                                    ccaTCAACGAGTTGATCGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCg               >  2:646654/3‑150 (MQ=255)
                                                                                                                                     gATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGc              >  2:994949/1‑150 (MQ=255)
                                                                                                                                       tCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGccc            >  2:2127159/1‑149 (MQ=255)
                                                                                                                                       tCAACTCGTTGATGGCCATGGTGGGTGGCACCCTATGAACTTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCa            <  1:1308647/150‑1 (MQ=255)
                                                                                                                                        cAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCAc           <  1:1279426/150‑1 (MQ=255)
                                                                                                                                          aCTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCAcca         >  1:735402/1‑149 (MQ=255)
                                                                                                                                            tCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtgg                                                               <  2:1722673/94‑1 (MQ=255)
                                                                                                                                            tCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtgg                                                               >  1:1722673/1‑94 (MQ=255)
                                                                                                                                              gTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACCGGCCt     >  2:138071/1‑150 (MQ=255)
                                                                                                                                               ttGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttt                                                      <  2:501540/100‑1 (MQ=255)
                                                                                                                                               ttGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttt                                                      >  1:501540/1‑100 (MQ=255)
                                                                                                                                                 gATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACCGGCCtggt  >  2:762998/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACCGGCCTGGT  >  NC_002947/2403997‑2404291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑