Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,560,507 G→C 8.5% W142C (TGG→TGC) ‡ PP_2243 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,560,5070GC 8.5% 215.7 / 8.3 70W142C (TGG→TGC) ‡PP_2243hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/31);  new base C (3/3);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGCTT  >  NC_002947/2560363‑2560653
                                                                                                                                                |                                                                                                                                                   
cGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCtggctggc                                                                                                                                                <  2:894185/150‑1 (MQ=255)
 gACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCGGGCTggcg                                                                                                                                               >  1:958694/1‑150 (MQ=255)
     ttGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCt                                                                                                                                           >  1:1720256/1‑150 (MQ=255)
     ttGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCt                                                                                                                                           <  2:1404074/150‑1 (MQ=255)
         gCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGa                                                                                                                                      >  2:1726847/1‑150 (MQ=255)
              ggCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGc                                                                                                                                 <  2:1949868/150‑1 (MQ=255)
               gCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCctggctgg                                                                                                                                                 <  2:1985228/134‑1 (MQ=255)
               gCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCctggctgg                                                                                                                                                 >  1:1985228/1‑134 (MQ=255)
                         tCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATTGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGActgg                                                                                                                      >  1:959934/1‑150 (MQ=255)
                         tCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCg                                                                                                                               >  1:1613060/1‑141 (MQ=255)
                         tCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCg                                                                                                                               <  2:1613060/141‑1 (MQ=255)
                           gTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTGTCGA‑AGGCCgcca                                                                                                                            <  2:1087814/142‑4 (MQ=255)
                           gTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTGTCGA‑AGGCCgcca                                                                                                                            >  1:1087814/1‑139 (MQ=255)
                            ttGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGActggctg                                                                                                                   <  1:157112/150‑1 (MQ=255)
                               aaGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCg                                                                                                                >  2:525562/1‑150 (MQ=255)
                                 gCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGt                                                                                                              >  2:1891811/1‑150 (MQ=255)
                                  cAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGt                                                                                                                            >  1:1324167/1‑135 (MQ=255)
                                  cAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGCGAGTCGTTCGCTCCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGt                                                                                                                            <  2:1324167/135‑1 (MQ=255)
                                   aaCTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGa                                                                                                            <  1:340546/150‑1 (MQ=255)
                                      tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGa                                                                                                                          >  2:2116901/1‑133 (MQ=255)
                                      tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGa                                                                                                                          <  1:2116901/133‑1 (MQ=255)
                                      tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                         >  1:2248721/1‑150 (MQ=255)
                                        cAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGacccac                                                                                                       <  2:2248721/150‑1 (MQ=255)
                                          aGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGActg                                                                                                                       >  2:488207/1‑132 (MQ=255)
                                          aGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGActg                                                                                                                       <  1:488207/132‑1 (MQ=255)
                                              gAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATg                                                                                                 >  2:93811/1‑150 (MQ=255)
                                              gAGCAACAGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATg                                                                                                 >  2:2108522/1‑150 (MQ=255)
                                                 cAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTc                                                                                              <  2:58308/150‑1 (MQ=255)
                                                   aCCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCAt                                                                                            <  1:1767572/150‑1 (MQ=255)
                                                     cGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCAAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATgc                                                                                          >  1:1772866/1‑150 (MQ=255)
                                                            gTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACACGACGGTCACGCGCGccct                                                                                   >  2:436258/1‑148 (MQ=255)
                                                                  cGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGt                                                                             <  2:2142881/150‑1 (MQ=255)
                                                                      tGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAg                                                                         <  2:958694/150‑1 (MQ=255)
                                                                      tGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAg                                                                         <  1:1726847/150‑1 (MQ=255)
                                                                          tGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCtg                                                                                                                                          >  1:2073594/1‑81 (MQ=38)
                                                                          tGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCtg                                                                                                                                          <  2:2073594/82‑2 (MQ=38)
                                                                              ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGa                                                                                                   <  1:1401191/116‑1 (MQ=255)
                                                                              ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGa                                                                                                   >  2:1401191/1‑116 (MQ=255)
                                                                              ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                 <  1:615234/150‑1 (MQ=255)
                                                                              ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                 <  1:93811/150‑1 (MQ=255)
                                                                              ggATCCGGCCAGTGCCGTGAATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCCGGTACAGGTGCTTgg                                                                 <  2:1661136/150‑1 (MQ=255)
                                                                                aTCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgggg                                                               >  2:1288238/1‑149 (MQ=255)
                                                                                aTCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgt                                                               >  2:2084869/1‑150 (MQ=255)
                                                                                aTCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgt                                                               <  2:1294452/150‑1 (MQ=255)
                                                                                 tCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgtg                                                              >  2:249017/1‑150 (MQ=255)
                                                                                    ggCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGGGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTcc                                                           <  2:470310/150‑1 (MQ=255)
                                                                                       cAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGAt                                                        >  2:1607470/1‑150 (MQ=255)
                                                                                              gTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCAGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGcc                                                 >  1:901367/1‑150 (MQ=255)
                                                                                               tGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCg                                                >  2:977922/1‑150 (MQ=255)
                                                                                                  aTGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCAAGGCCGCCAGCCAGGCGGCCtggc                                                                                                                                        >  1:2327547/1‑57 (MQ=18)
                                                                                                  aTGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCAAGGCCGCCAGCCAGGCGGCCtggc                                                                                                                                        <  2:2327547/60‑4 (MQ=18)
                                                                                                    ggtggtGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAg                                           <  1:1891811/150‑1 (MQ=255)
                                                                                                      tggtgAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGt                                                                                                              <  2:983048/81‑1 (MQ=255)
                                                                                                      tggtgAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGt                                                                                                              >  1:983048/1‑81 (MQ=255)
                                                                                                          gAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCAT‑TTGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGAGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATc                                     <  1:436258/150‑1 (MQ=255)
                                                                                                           aGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTAAAGGTGCTTGGGTGTCCGATGAGTGCCGGGCa                                            >  1:2426551/1‑142 (MQ=255)
                                                                                                           aGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTAAAGGTGCTTGGGTGTCCGATGAGTGCCGGGCa                                            <  2:2426551/142‑1 (MQ=255)
                                                                                                             tCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGAc                                  <  1:525562/150‑1 (MQ=255)
                                                                                                             tCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGAc                                  <  1:977922/150‑1 (MQ=255)
                                                                                                               gTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGt                                >  1:575960/1‑150 (MQ=255)
                                                                                                                    cTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTGTCGACGtgctgc                           <  1:2084869/150‑1 (MQ=255)
                                                                                                                    cTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCGTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGtgctgc                           >  2:1638133/1‑150 (MQ=255)
                                                                                                                          tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCtt                                                                   <  2:2156049/104‑1 (MQ=255)
                                                                                                                          tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCtt                                                                   >  1:2156049/1‑104 (MQ=255)
                                                                                                                          tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGt                     <  1:673289/150‑1 (MQ=255)
                                                                                                                             gCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCAGGGTAGCGTATCGACGTGCTGCCGCTGTCAc                  <  1:1638133/150‑1 (MQ=255)
                                                                                                                               cAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGt                >  1:763400/1‑150 (MQ=255)
                                                                                                                                     cAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTTACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGGGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCaa          >  1:2210396/1‑150 (MQ=255)
                                                                                                                                     aaGGCCGCCAGCCAGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGGCGTGCTGCCGCTGTCACGTCTGCaa          >  1:604647/2‑150 (MQ=255)
                                                                                                                                        gccgccTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTCCTGCCGCTGTCACGTCTGCAATTg       >  2:1219946/1‑150 (MQ=255)
                                                                                                                                         ccgccTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGa      >  2:1431379/1‑150 (MQ=255)
                                                                                                                                          cgccTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAg     <  1:1607470/150‑1 (MQ=255)
                                                                                                                                           gccTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGc    >  1:1340038/1‑150 (MQ=255)
                                                                                                                                             ctggctggCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGcct  >  2:1027/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGCTT  >  NC_002947/2560363‑2560653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑