Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,638,137 T→G 5.4% V67G (GTG→GGG)  PP_2308 → acyl‑CoA thioesterase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,638,1370TG 5.4% 270.1 / 6.2 93V67G (GTG→GGG) PP_2308acyl‑CoA thioesterase II
Reads supporting (aligned to +/- strand):  ref base T (42/46);  new base G (3/2);  total (45/48)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.54e-01

CAGCATTCCGCCCAGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCGGTGGTCGAAGTGC  >  NC_002947/2637988‑2638277
                                                                                                                                                       |                                                                                                                                            
cAGCATTCCGCCCAGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGAtgt                                                                                                                                              <  1:1380436/150‑1 (MQ=255)
 aGCATTCCGCCCAGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGAtgtg                                                                                                                                             <  2:872871/150‑1 (MQ=255)
     ttCCGCCCAGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCa                                                                                                                                         >  2:1621024/1‑150 (MQ=255)
       tctcccAGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGCCAACCGCCCGGTACGCT‑CATTTTCCATCAT‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATc                                                                                                                                       <  1:647888/147‑1 (MQ=255)
        cGCCCAGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATcc                                                                                                                                      >  2:879707/1‑150 (MQ=255)
         gCCCAGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCg                                                                                                                                     >  2:529280/1‑150 (MQ=255)
           ccAGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATccaga                                                                                                                                   >  2:647888/1‑147 (MQ=255)
             aGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTc                                                                                                                                 >  2:314291/1‑150 (MQ=255)
                tGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAg                                                                                                                              <  2:1699522/150‑1 (MQ=255)
                  ggCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGt                                                                                                                            <  2:554273/150‑1 (MQ=255)
                   gCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTg                                                                                                                           >  2:1087169/1‑150 (MQ=255)
                     ccAGGGGCGCGCTGCCTTTGGCGGGATCATGGCGGACACGGCCGATGAAACCATGCGCCTCAAGATCAGCGAGTGCCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGa                                                                                                                         <  1:1738818/150‑1 (MQ=255)
                      cAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAg                                                                                                                        <  1:758381/150‑1 (MQ=255)
                           gcgcgcTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGtttt                                                                                                                   >  2:2403229/1‑150 (MQ=255)
                           gcgcgcTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGtttt                                                                                                                   <  1:1621024/150‑1 (MQ=255)
                           gcgcgcTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGtttt                                                                                                                   <  2:1757311/150‑1 (MQ=255)
                              cgcTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTgcg                                                                                                                >  1:1015817/1‑150 (MQ=255)
                                 tGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAgg                                                                                                                             >  1:2222013/1‑134 (MQ=255)
                                 tGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAgg                                                                                                                             <  2:2222013/134‑1 (MQ=255)
                                 tGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGa                                                                                                             >  2:1660970/1‑150 (MQ=255)
                                  gCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaa                                                                                                            <  1:650244/150‑1 (MQ=255)
                                  gCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCAACCGTGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaa                                                                                                            <  1:551208/150‑1 (MQ=255)
                                     tttGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaaggc                                                                                                         >  2:1602953/1‑150 (MQ=255)
                                     tttGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaaggc                                                                                                         >  2:2203354/1‑150 (MQ=255)
                                             gCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGt                                                                                                 <  1:954143/150‑1 (MQ=255)
                                                gATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAg                                                                                              <  1:1471163/150‑1 (MQ=255)
                                                  tGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaaggcaag                                                                                                      <  2:1089467/140‑1 (MQ=255)
                                                  tGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaaggcaag                                                                                                      >  1:1089467/1‑140 (MQ=255)
                                                    gcggcCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGG‑AATTAGTGTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACg                                                                                          <  1:1333400/150‑1 (MQ=255)
                                                      ggcCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGtt                                                                                        >  2:1673200/1‑150 (MQ=255)
                                                        ccATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaaggcaa                                                                                                       >  1:182592/1‑133 (MQ=255)
                                                        ccATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaaggcaa                                                                                                       <  2:182592/133‑1 (MQ=255)
                                                          aTGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTg                                                                                    >  2:2219509/1‑150 (MQ=255)
                                                                tATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCcgc                                                                              >  2:724694/1‑150 (MQ=255)
                                                                tATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCcgc                                                                              <  2:1837553/150‑1 (MQ=255)
                                                                 aTGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCcgcg                                                                             >  2:407636/1‑150 (MQ=255)
                                                                 aTGAAACCATGCGCCTCAAGATCAGCCCCAACCCCCAGGTAAAATGCGACTTTCAT‑AA‑CCTCGTGGCACTCTCGGCGGCGGATGTGCCCATCCGCTTCGAGGGGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCcgcg                                                                             >  1:1863959/1‑150 (MQ=255)
                                                                    aaaCCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaa                                                                                                            >  1:526257/1‑116 (MQ=255)
                                                                    aaaCCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGaa                                                                                                            <  2:526257/116‑1 (MQ=255)
                                                                      aCCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGtt                                                                        >  1:929427/1‑150 (MQ=255)
                                                                       ccATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTc                                                                       <  1:529280/150‑1 (MQ=255)
                                                                        cATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTgcgc                                                                                                               <  1:1335662/109‑1 (MQ=255)
                                                                        cATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTgcgc                                                                                                               >  2:1335662/1‑109 (MQ=255)
                                                                        cATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCa                                                                      <  1:1246843/150‑1 (MQ=255)
                                                                        cATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCa                                                                      <  1:1884671/150‑1 (MQ=255)
                                                                         aTGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGACCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAg                                                                     >  1:2179614/1‑150 (MQ=255)
                                                                            cgcCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGAt                                                                  <  1:1087169/150‑1 (MQ=255)
                                                                              ccTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCAGCGGCGGCGGATGTGGCTTGTCGCTTCTAGGTGGGGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATgg                                                                >  1:289948/1‑150 (MQ=255)
                                                                               cTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGc                                                               <  1:1653628/150‑1 (MQ=255)
                                                                               cTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGc                                                               >  2:1148860/1‑150 (MQ=255)
                                                                                tCAAGATCAGCGACAACCGCCCGGTACGCT‑AATTGGCCATCAG‑CTTCGTGGAACTCGCGGCGGCGGATGTCCCCATCCGGTTCGAAGAGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCGGGGCGGCGCTGTTCAGGATGGcc                                                              >  1:714746/1‑150 (MQ=255)
                                                                                  aaGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGtt                                                                                        >  2:1652872/1‑122 (MQ=255)
                                                                                  aaGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGtt                                                                                        <  1:1652872/122‑1 (MQ=255)
                                                                                  aaGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAg                                                            <  2:1863959/150‑1 (MQ=255)
                                                                                  aaGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAg                                                            <  1:407636/150‑1 (MQ=255)
                                                                                  aaGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAg                                                            >  1:1191587/1‑150 (MQ=255)
                                                                                       cAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAggtggt                                                       <  1:954410/150‑1 (MQ=255)
                                                                                         gCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCgcgc                                                                            >  2:936388/1‑127 (MQ=255)
                                                                                         gCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCgcgc                                                                            <  1:936388/127‑1 (MQ=255)
                                                                                         gCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGa                                                     <  1:1054704/150‑1 (MQ=255)
                                                                                               aCCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCg                                                                                                   <  2:1607430/98‑1 (MQ=255)
                                                                                               aCCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCg                                                                                                   >  1:1607430/1‑98 (MQ=255)
                                                                                                 cGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTg                                             >  1:590968/1‑150 (MQ=255)
                                                                                                 cGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTg                                             >  2:200536/1‑150 (MQ=255)
                                                                                                    ccGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAg                                          <  1:314291/150‑1 (MQ=255)
                                                                                                      ggTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGAGGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAggg                                        <  2:444190/150‑1 (MQ=255)
                                                                                                          cGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAAt                                    <  2:613609/150‑1 (MQ=255)
                                                                                                           gCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAAtt                                   >  2:2122173/1‑150 (MQ=255)
                                                                                                             t‑CATTGGCCATCAG‑CTTCGTGGCACCGGAATCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTc                                 >  1:665661/1‑150 (MQ=255)
                                                                                                                aTTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCGAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAgg                                         <  2:213927/140‑1 (MQ=255)
                                                                                                                aTTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCGAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAgg                                         >  1:213927/1‑140 (MQ=255)
                                                                                                                aTTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAgg                                         <  1:670427/140‑1 (MQ=255)
                                                                                                                aTTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAgg                                         >  2:670427/1‑140 (MQ=255)
                                                                                                                aTTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCgg                               >  1:1607453/1‑150 (MQ=255)
                                                                                                                 ttGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGt                              >  1:86196/1‑150 (MQ=255)
                                                                                                                 ttGGCCATCAG‑CTTCGTCAGACGCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGt                              <  1:1306335/150‑1 (MQ=255)
                                                                                                                    gCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTCGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAggtggt                                                       >  2:2278154/1‑122 (MQ=255)
                                                                                                                    gCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTCGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAggtggt                                                       <  1:2278154/122‑1 (MQ=255)
                                                                                                                     ccATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCgg                          <  1:2203354/150‑1 (MQ=255)
                                                                                                                           g‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGgccgcc                    >  2:1645039/1‑150 (MQ=255)
                                                                                                                              ttCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCt                  >  2:1637719/1‑150 (MQ=255)
                                                                                                                                cGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTc                <  2:929427/150‑1 (MQ=255)
                                                                                                                                   ggCCCCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTCGTGCAGGGCAATTTCGGTGCGCGCCGTCCTTCggt             <  2:690637/150‑1 (MQ=255)
                                                                                                                                   ggCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGCGGCAGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCggt             <  1:724694/150‑1 (MQ=255)
                                                                                                                                   ggCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCggt             <  1:1602953/150‑1 (MQ=255)
                                                                                                                                   ggCACCCGCGGCGGCGGATGTGCCCATCAGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCggt             <  1:182875/150‑1 (MQ=255)
                                                                                                                                     cACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCggtgg           >  2:1050866/1‑150 (MQ=255)
                                                                                                                                          gcggcggcgGATGTGCCTATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCGGTGGTCGaa      <  2:289948/150‑1 (MQ=255)
                                                                                                                                          gcggcggcgGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCGGTGGTCGaa      <  1:671507/150‑1 (MQ=255)
                                                                                                                                          gcggcggcgGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCGGTGGTCGaa      <  2:1015817/150‑1 (MQ=255)
                                                                                                                                          ctcgaagcggATGGGCACATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCGGTGGTCGaa      >  2:2148621/7‑150 (MQ=255)
                                                                                                                                           tcgaagcggATGGGCACATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTg                             >  2:1929533/6‑126 (MQ=255)
                                                                                                                                           tcgaagcggATGGGCACATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTg                             <  1:1929533/121‑1 (MQ=255)
                                                                                                                                           tcgaagcggATGGGCACATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCGGTGGTCGAAg     >  2:5164/6‑150 (MQ=255)
                                                                                                                                              aagcggATGGGCACATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCGGTGGTCGAAGTGc  <  1:2148621/148‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                            
CAGCATTCCGCCCAGCTGGGCCCAGGGGCGCGCTGCCTTTGGCGGGCTGATGGCGGCCATGGTCTATGAAACCATGCGCCTCAAGATCAGCGACAACCGCCCGGTACGCT‑CATTGGCCATCAG‑CTTCGTGGCACCCGCGGCGGCGGATGTGCCCATCCGCTTCGAGGTGGAGGTTTTGCGCGAAGGCAAGGCGGTTAGCACGTTGCTGGGCCGCGCTGTTCAGGATGGCCAGGTGGTGACTTTGGTGCAGGGCAATTTCGGTGCGGGCCGCCCTTCGGTGGTCGAAGTGC  >  NC_002947/2637988‑2638277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑