Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,650,131 C→A 7.5% intergenic (+27/+107) PP_2320 → / ← oprI hypothetical protein/major outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,650,1310CA 7.5% 155.0 / 3.0 54intergenic (+27/+107)PP_2320/oprIhypothetical protein/major outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base A (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

ACTGGGACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCG  >  NC_002947/2649983‑2650276
                                                                                                                                                    |                                                                                                                                                 
aCTGGGACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGccc                                                                                                                                                  >  1:385793/1‑150 (MQ=255)
   gggACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTa                                                                                                                                               <  1:1237563/150‑1 (MQ=255)
    ggACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAg                                                                                                                                              >  1:1033361/1‑150 (MQ=255)
     gACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGt                                                                                                                                             <  1:838076/150‑1 (MQ=255)
      aCATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTg                                                                                                                                            >  2:2058650/1‑150 (MQ=255)
         tGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAg                                                                                                                                         <  2:1033361/150‑1 (MQ=255)
          ggTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAgg                                                                                                                                        >  1:1874823/1‑150 (MQ=255)
           gTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTa                                                                                                                                               >  1:1439278/1‑142 (MQ=255)
           gTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTa                                                                                                                                               <  2:1439278/142‑1 (MQ=255)
           gTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGc                                                                                                                                       <  2:374652/150‑1 (MQ=255)
             gCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCACTAAGGGCAGGCCt                                                                                                                                     <  2:453788/150‑1 (MQ=255)
             gCGTGACGTGGTGGCTGCAGAAGATGGTATGACGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCt                                                                                                                                     >  2:737774/1‑150 (MQ=255)
                tGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCtttt                                                                                                                                  >  1:847264/1‑150 (MQ=255)
                     tggtggCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAGCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTAc                                                                                                                             <  2:1638182/150‑1 (MQ=255)
                     tggtggCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTAc                                                                                                                             <  1:880768/150‑1 (MQ=255)
                             gCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTg                                                                                                                                            >  2:465783/1‑127 (MQ=255)
                             gCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTg                                                                                                                                            <  1:465783/127‑1 (MQ=255)
                              cAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCGTGTCGTACCTGCTGAcc                                                                                                                    >  2:1838934/1‑150 (MQ=255)
                                gaagaTGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACcgc                                                                                                                  <  1:737774/150‑1 (MQ=255)
                                 aagaTGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACcgcg                                                                                                                 >  2:1191910/1‑150 (MQ=255)
                                            ccGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGttt                                                                                                      <  2:1874823/150‑1 (MQ=255)
                                                 aGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAg                                                                                                 <  2:1022285/150‑1 (MQ=255)
                                                 aGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAg                                                                                                 <  1:2058650/150‑1 (MQ=255)
                                                 aGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAg                                                                                                 <  1:1381648/150‑1 (MQ=255)
                                                 aGAAATTGCCGTGCCCGTGGACAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAg                                                                                                 <  2:553323/150‑1 (MQ=255)
                                                      ttGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGcc                                                                                                                                                   <  1:577359/95‑1 (MQ=255)
                                                      ttGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGcc                                                                                                                                                   >  2:577359/1‑95 (MQ=255)
                                                                 gTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGa                                                                                 >  2:2106838/1‑150 (MQ=255)
                                                                        aCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACaa                                                                          <  2:577670/150‑1 (MQ=255)
                                                                         ccAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACctgct                                                                                                                        >  2:616919/1‑103 (MQ=255)
                                                                         ccAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACctgct                                                                                                                        <  1:616919/103‑1 (MQ=255)
                                                                          cAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGcc                                                                                   <  2:2337348/139‑1 (MQ=255)
                                                                          cAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGcc                                                                                   >  1:2337348/1‑139 (MQ=255)
                                                                            gggggCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTgg                                                                      <  1:584474/150‑1 (MQ=255)
                                                                                  cGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGc                                                                >  1:323002/1‑150 (MQ=255)
                                                                                      ccGATGGTGACGAGCATGCCGCCGGCACTGCAGTAAGGTAAGTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAAGAAAAAAGCCGACCCACAAGTGGGTCGGcttct                                                            <  1:880436/150‑1 (MQ=255)
                                                                                          tGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGcttttttat                                                        <  1:1810362/150‑7 (MQ=255)
                                                                                           ggTGACGAGCATTCCGCCTGAACTGCAGTACGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGcttttttatt                                                       <  1:2180108/150‑5 (MQ=255)
                                                                                                 gAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCgc                                                 >  2:2389295/1‑149 (MQ=255)
                                                                                                      ttCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGtgatga                                            >  1:1216542/1‑150 (MQ=255)
                                                                                                       tCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGat                                           >  2:1896155/1‑150 (MQ=255)
                                                                                                          gCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAgg                                                                                                <  2:2007419/94‑1 (MQ=255)
                                                                                                          gCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAgg                                                                                                >  1:2007419/1‑94 (MQ=255)
                                                                                                            tcGAACTGCAGTAAGGTAATTTCGGCGTCCCACGGACCGCTTTTAGTGTAGGCCTTTTCGTATCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGAttatta                                      <  2:390959/149‑1 (MQ=255)
                                                                                                              gAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACt                                    >  1:789575/1‑150 (MQ=255)
                                                                                                                     aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGc                                                                <  2:1642690/115‑1 (MQ=255)
                                                                                                                     aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGc                                                                >  1:1642690/1‑115 (MQ=255)
                                                                                                                     aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttctt                                                           >  2:619609/1‑120 (MQ=255)
                                                                                                                     aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttctt                                                           <  1:619609/120‑1 (MQ=255)
                                                                                                                      gTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctg                            >  2:1994812/1‑150 (MQ=255)
                                                                                                                                          ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg        >  1:1870825/1‑150 (MQ=255)
                                                                                                                                          ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg        >  2:1291895/1‑150 (MQ=255)
                                                                                                                                          atgagCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg        >  1:1663636/5‑150 (MQ=255)
                                                                                                                                           aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCg                                                                  >  2:2243821/2‑91 (MQ=39)
                                                                                                                                           aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCg                                                                  <  1:2243821/90‑1 (MQ=39)
                                                                                                                                              gCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgcg    >  2:158008/1‑150 (MQ=255)
                                                                                                                                                cTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgcgcg  >  1:774630/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTGGGACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCG  >  NC_002947/2649983‑2650276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑