| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,793,795 | G→T | 11.9% | intergenic (+61/+66) | PP_2445 → / ← PP_2446 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,793,795 | 0 | G | T | 11.9% | 123.7 / 5.2 | 42 | intergenic (+61/+66) | PP_2445/PP_2446 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (15/22); new base T (3/2); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGA > NC_002947/2793653‑2793942 | cATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACt < 1:225565/148‑1 (MQ=255)cATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACt > 2:225565/1‑148 (MQ=255) gTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGCTGTTAACAGGGGTGCCACCCGCCCCTCGTCCTGGCAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAAcgc < 1:647175/150‑1 (MQ=255) gTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAAcgc < 1:2425816/150‑1 (MQ=255) tctcGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCa < 2:2416847/144‑1 (MQ=255) tctcGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCa > 1:2416847/1‑144 (MQ=255) tctcGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGa < 2:614817/150‑1 (MQ=255) ctcGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGaa < 1:1072148/150‑1 (MQ=255) ctcGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGaa < 2:31772/150‑1 (MQ=255) gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATAc > 2:1435623/1‑150 (MQ=255) gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATAc < 1:2307887/150‑1 (MQ=255) gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATAc < 2:2382452/150‑1 (MQ=255) gACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGAACAGGTATTCGCGTGGCACTG‑GCAACGCGAATAc < 1:1677189/150‑1 (MQ=255) cGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCt < 1:2010946/150‑1 (MQ=255) cGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCt < 2:2384973/150‑1 (MQ=255) cGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCt < 2:208290/150‑1 (MQ=255) tATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGCCAGT‑GCCACGCGAAt > 2:2229238/1‑142 (MQ=38) ggTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCggg < 1:1435623/150‑1 (MQ=255) aaCATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAAt < 2:375809/129‑1 (MQ=255) aaCATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAAt > 1:375809/1‑129 (MQ=255) ccGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGC‑CAGTGCCACGCGAATACCtg > 1:1285057/1‑123 (MQ=25) ccGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGC‑CAGTGCCACGCGAATACCtg < 2:1285057/123‑1 (MQ=25) ccGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGC‑CAGTGCCACGCGAATACCtg > 1:1297088/1‑123 (MQ=25) ccGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTTGC‑CAGTGCCACGCGAATACCtg < 2:1297088/123‑1 (MQ=25) tAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTg‑g < 2:1711305/66‑1 (MQ=255) tAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTg‑g > 1:1711305/1‑66 (MQ=255) aaaaaGGGGCGACTCGCGACCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCgggg > 2:585922/1‑150 (MQ=255) aaaGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTACAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGcc > 2:932170/1‑150 (MQ=255) ggggCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCg > 2:1560029/1‑82 (MQ=255) ggggCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCg < 1:1560029/82‑1 (MQ=255) ttGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTACAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGc < 1:932170/150‑1 (MQ=255) ccGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCgg > 2:2052317/1‑150 (MQ=255) gCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTg < 1:2052317/150‑1 (MQ=255) tGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAg > 2:1557713/1‑87 (MQ=255) tGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAg < 1:1557713/87‑1 (MQ=255) caGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggt > 2:699147/1‑150 (MQ=255) gTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAggtggt < 1:699147/150‑1 (MQ=255) aTTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGgtgt > 2:2015720/1‑150 (MQ=255) ttCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGtgtg > 2:1373785/1‑150 (MQ=255) gTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGa > 1:1937924/1‑150 (MQ=255) tGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTgg > 1:1501421/1‑106 (MQ=255) tGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTgg < 2:1501421/106‑1 (MQ=255) | CATGGCGTTCTCGACGGCTATCGAGGTCCTCAACATCCTGGCCCGTCGAGCGCGTTTGAAGCGTGAGGCGGCTGAAGGTTGATGTAAAAAGGGGCGCCTCGCGCCCCTCTTGCGGACAAGCCCGCTGCACAGGTATTCGCGTGGCACTG‑GCAACGCGAATACCTGTGCAGCGGGCTTGTCAGTCAGTGGGCTTCGGGAAATCCTGAGGTTAGCTCACCCCGGCATGCCGCCGGGGCCGTCTTACTGGCTGTGGCTTGGCGGGTGCCTGCGGTGGCAGGTGGTGTGCATGA > NC_002947/2793653‑2793942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |