Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,873,691 T→C 6.2% V143A (GTG→GCG) ‡ cigR → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,873,6910TC 6.2% 283.9 / 5.8 97V143A (GTG→GCG) ‡cigRmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (39/52);  new base C (2/4);  total (41/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

CCTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACC  >  NC_002947/2873543‑2873837
                                                                                                                                                    |                                                                                                                                                   
ccTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGGCCTGATTCTGGTCGCCGTg                                                                                                                                                    >  1:2244307/1‑150 (MQ=255)
ccTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTg                                                                                                                                                    <  2:1150720/150‑1 (MQ=255)
 cTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                                   >  1:1063620/1‑150 (MQ=255)
 cTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                                   >  2:695649/1‑150 (MQ=255)
  tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGCCGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGc                                                                                                                                                  <  1:511452/150‑1 (MQ=255)
  tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGc                                                                                                                                                  >  1:1395242/1‑150 (MQ=255)
  tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGc                                                                                                                                                  >  2:1122417/1‑150 (MQ=255)
  tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGc                                                                                                                                                  >  2:168811/1‑150 (MQ=255)
  tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGGCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGc                                                                                                                                                  >  2:2299522/1‑150 (MQ=255)
       gCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCg                                                                                                                                             >  1:2148971/1‑150 (MQ=255)
          ttCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGCCCGGGa                                                                                                                                          <  2:224918/150‑1 (MQ=255)
          ttCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATCCCGGTCGCCACGGCGAccagaa                                                                                                                                          <  1:1667303/150‑5 (MQ=255)
             agaagaACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatca                                                                                                                                       >  2:707956/1‑150 (MQ=255)
             agaagaACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatca                                                                                                                                       >  2:2182038/1‑150 (MQ=255)
                 gaaCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatc                                                                                                                                        >  1:1169398/1‑145 (MQ=255)
                 gaaCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatc                                                                                                                                        <  2:1169398/145‑1 (MQ=255)
                   aCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCGAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATg                                                                                                                                 >  1:608247/1‑150 (MQ=255)
                     cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAg                                                                                                                               >  1:2445719/1‑150 (MQ=255)
                       ggCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgt                                                                                                                             <  1:940127/150‑1 (MQ=255)
                       ggCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgt                                                                                                                             <  1:311313/150‑1 (MQ=255)
                         cccGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCACCGTGGCGACCGGGATCATCTATGAGgtgt                                                                                                                           <  2:2263352/150‑1 (MQ=255)
                          ccGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGCCCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGtt                                                                                                                          <  1:1133893/150‑1 (MQ=255)
                          ccGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGtt                                                                                                                          <  1:1808508/150‑1 (MQ=255)
                              ggCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAAc                                                                                                                      >  1:308170/1‑150 (MQ=255)
                               gCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGCAGTCTCTGTTGTCATCTGCCTCACTACGAAGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACg                                                                                                                     <  1:1740570/150‑1 (MQ=255)
                                 aaGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGTTCATGTATTAGTTGTTTTACggg                                                                                                                   <  2:147541/150‑1 (MQ=255)
                                 aaGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACggg                                                                                                                   <  2:2445719/150‑1 (MQ=255)
                                      ccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgtg                                                                                                                            >  1:305881/1‑136 (MQ=255)
                                      ccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgtg                                                                                                                            <  2:305881/136‑1 (MQ=255)
                                         gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                           <  1:75629/150‑1 (MQ=255)
                                         gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                           <  1:1092611/150‑1 (MQ=255)
                                           cACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGtt                                                                                                                          <  2:2391382/133‑1 (MQ=255)
                                           cACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGtt                                                                                                                          >  1:2391382/1‑133 (MQ=255)
                                           cACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACt                                                                                                         >  2:421711/1‑150 (MQ=255)
                                            aCCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGGCCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTa                                                                                                        <  2:2244307/150‑1 (MQ=255)
                                             ccTGGTGTCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgt                                                                                                                             <  1:1977123/128‑1 (MQ=255)
                                             ccTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgt                                                                                                                             >  2:1977123/1‑128 (MQ=255)
                                             ccTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTaa                                                                                                       <  1:695649/150‑1 (MQ=255)
                                             ccTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTaa                                                                                                       <  1:176198/150‑1 (MQ=255)
                                             ccTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTaa                                                                                                       <  1:2081027/150‑1 (MQ=255)
                                                   aTCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGc                                                                                                 <  1:2182038/150‑1 (MQ=255)
                                                     cGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAg                                                                                               <  1:1502194/150‑1 (MQ=255)
                                                       ccAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTg                                                                                                                         >  2:425164/1‑122 (MQ=255)
                                                       ccAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTg                                                                                                                         <  1:425164/122‑1 (MQ=255)
                                                       ccAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGt                                                                                             <  2:304544/150‑1 (MQ=255)
                                                       ccAAGAAGCTGGATGGTCGTCTGCTGGCGCAGCTCCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGt                                                                                             <  1:1902681/150‑1 (MQ=255)
                                                           gaagCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGa                                                                                         <  1:1122417/150‑1 (MQ=255)
                                                           gaagCTGGATGGTCGTCTGTTCGGTAAGCTGCGTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGCCCGGGCTCATCTATGAGTTGTTGAACCTAGCGTTCTACTAAGGCTGCAGGTGCGa                                                                                         <  2:1207031/150‑1 (MQ=255)
                                                                tGGATGGTCGCCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCACGCGGGCGGAGTTCTGATGCTGGCTGTCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGt                                                                                    <  1:168811/150‑1 (MQ=255)
                                                                 ggATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTc                                                                                   <  1:1590011/150‑1 (MQ=255)
                                                                      gtcgtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTc                                                                              <  2:1140077/150‑1 (MQ=255)
                                                                       tcgtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCg                                                                             >  2:1983646/1‑150 (MQ=255)
                                                                       tcgtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCg                                                                             >  2:1619951/1‑150 (MQ=255)
                                                                         gtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCTACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGt                                                                           >  2:1848982/1‑150 (MQ=255)
                                                                             gTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCt                                                                       <  2:2148971/150‑1 (MQ=255)
                                                                             gTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCt                                                                       <  1:421711/150‑1 (MQ=255)
                                                                              ttGGGGCAGCCGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTg                                                                      >  2:2130650/1‑150 (MQ=255)
                                                                                ggggCAGCTGCCTCACTACGACGGGTAGGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCa                                                                    <  1:713599/150‑1 (MQ=255)
                                                                                 gggCAGCTGCCTCACTACGACGGGTATGAGTGGATGTAAGCGGGCGCAGACCTGATTCTGGTCGCCGTTTCCAGAGCGATAAGGCGTTATTCTGTGAACTGAGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCAGCTCGGTCCCTGCAg                                                                   >  1:168676/1‑150 (MQ=255)
                                                                                 gggCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAg                                                                   <  1:2067494/150‑1 (MQ=255)
                                                                                   gCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGgc                                                                 >  1:76655/1‑150 (MQ=255)
                                                                                     aGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGgcgc                                                               <  1:1983646/150‑1 (MQ=255)
                                                                                      gCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGCGGCTTTACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCt                                                              <  1:1842279/150‑1 (MQ=255)
                                                                                       cTGCATCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGTCGACCGGGATCATCTATGAGGTGTTAAAAGTAGCATTCGACTAAGGCCGAAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTg                                                             >  1:1595378/1‑150 (MQ=255)
                                                                                        tGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTgg                                                            >  2:469371/1‑150 (MQ=255)
                                                                                           cTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGt                                                         >  1:458533/1‑150 (MQ=255)
                                                                                            tCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTg                                                        >  2:231163/1‑150 (MQ=255)
                                                                                            tCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTg                                                        >  2:1858695/1‑150 (MQ=255)
                                                                                             cACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGc                                                       <  1:469371/150‑1 (MQ=255)
                                                                                              aCTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGcc                                                      <  1:1619951/150‑1 (MQ=255)
                                                                                                 acgacgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGc                                                   <  2:1063620/150‑1 (MQ=255)
                                                                                                    acgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGAt                                                <  1:707956/150‑1 (MQ=255)
                                                                                                     cgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATg                                               <  1:1268177/150‑1 (MQ=255)
                                                                                                      gggTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATgg                                              <  1:2299522/150‑1 (MQ=255)
                                                                                                             aGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCTGACGCTGGCGTGCCTGCGATGGGCTgc‑gc                                      >  1:1819307/1‑150 (MQ=255)
                                                                                                               tGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑gcag                                    >  1:1791747/1‑150 (MQ=255)
                                                                                                               tGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑gcag                                    <  1:861408/150‑1 (MQ=255)
                                                                                                                 gATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGt                                                         <  2:724996/128‑1 (MQ=255)
                                                                                                                 gATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑gcagca                                  >  1:1624304/1‑150 (MQ=255)
                                                                                                                 gATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTACCTGCAGGCGCTGGCGt                                                         >  1:724996/1‑128 (MQ=255)
                                                                                                                      aaGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCa                             >  2:1131158/1‑150 (MQ=255)
                                                                                                                       aGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGTTGGGCTGCTGC‑GCAGCCCat                            <  2:76655/150‑2 (MQ=255)
                                                                                                                        gCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGTTGGGCTGCTGC‑GCAGCCCatc                           <  2:1137261/150‑3 (MQ=255)
                                                                                                                           ggCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGTTGGGCTGC‑TGCGCAGCCCATCgca                        <  2:1224706/150‑3 (MQ=255)
                                                                                                                                   ccTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAAc                <  1:1491893/150‑1 (MQ=255)
                                                                                                                                       aTTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTg            <  2:1791747/150‑1 (MQ=255)
                                                                                                                                         tCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGcc          <  1:1131158/150‑1 (MQ=255)
                                                                                                                                         cccggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTcc                                                                                  <  2:313384/76‑1 (MQ=255)
                                                                                                                                         cccggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTcc                                                                                  >  1:313384/4‑79 (MQ=255)
                                                                                                                                         cccggTCGCCACGGCGACCAGAATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGcc          <  1:2451344/147‑1 (MQ=255)
                                                                                                                                           tgatCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGt        >  2:2313961/4‑150 (MQ=255)
                                                                                                                                           cggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATggg                                             <  2:1566011/113‑1 (MQ=255)
                                                                                                                                           cggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATggg                                             >  1:1566011/2‑114 (MQ=255)
                                                                                                                                             gTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTaa      >  2:1478208/1‑150 (MQ=255)
                                                                                                                                              tCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑Gcagcag                                 <  2:243612/122‑1 (MQ=255)
                                                                                                                                              tCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑Gcagcag                                 >  1:243612/1‑122 (MQ=255)
                                                                                                                                                 ccGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACAcc  >  1:18157/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACC  >  NC_002947/2873543‑2873837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑