BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,890,476	C→G	6.6%	E59D (GAG→GAC)	PP_2544 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,890,476	0	C	G	6.6%	326.7 / 12.7	106	E59D (GAG→GAC)	PP_2544	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (52/47); new base G (3/4); total (55/51)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

Only 100 of 108 total aligned reads displayed.

ACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGT  >  NC_002947/2890332‑2890625

aCCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCt                                                                                                                                                       >  2:2306429/1‑150 (MQ=255)

 ccATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGAACAGGTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCGtt                                                                                                                                                      >  1:626820/1‑150 (MQ=255)

  cATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTg                                                                                                                                                     <  1:630158/150‑1 (MQ=255)

   aTGGCCTTCATGCCTGACTACCAGATCCTGGGCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgg                                                                                                                                                    <  2:2378612/150‑1 (MQ=255)

     ggCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgggg                                                                                                                                                  >  1:1627447/1‑150 (MQ=255)

        cTTCATGCCTGACTACCAGATCCTGGACATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGAAACCGGCCGGCTCCCTTGGGG‑‑Acc                                                                                                                                             >  2:1209960/1‑150 (MQ=255)

         ttCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑Accc                                                                                                                                            <  2:1558184/150‑1 (MQ=255)

          tCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCg                                                                                                                                           <  1:2444844/150‑1 (MQ=255)

             tGCCAGACTACCAGATCCTGGTCATTTCCAACGGTATCGCAGCGGCGGTCGGGATGGCGGTGGGTGACGTGGACCCGATCCGCTCTGGTAGCAAGCAGCACTAAGCATCACTAGCCTCTGGAAACGGCCGGCTCACTTGGGG‑‑ACCCCGcc                                                                                                                                        >  2:1121266/1‑150 (MQ=255)

                  gACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTc                                                                                                                                   >  2:305028/1‑150 (MQ=255)

                    cTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTGGTGGTGGCGATGGGTGTGGTGCATCAAAGGCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcgc                                                                                                                                 <  1:1614156/150‑1 (MQ=255)

                    cTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcgc                                                                                                                                 <  2:2350613/150‑1 (MQ=255)

                     tACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgg                                                                                                                                                    >  2:2080427/1‑132 (MQ=255)

                     tACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgg                                                                                                                                                    <  1:2080427/132‑1 (MQ=255)

                      aCCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGGCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTGACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGTTTccaga                                                                                                                               <  2:2166902/150‑5 (MQ=38)

                       ccAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgtt                                                                                                                              >  1:341646/1‑150 (MQ=255)

                       ccAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgtt                                                                                                                              >  1:1271535/1‑150 (MQ=255)

                       ccAGATCCTGGTCATTTCCACCGGTATCACTGCGGCGGTCGTGGTGGCGATGGGTGGGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCGCTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgtt                                                                                                                              >  2:1255026/1‑150 (MQ=255)

                          gATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTATGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgttgtt                                                                                                                           >  1:2188895/1‑150 (MQ=255)

                              cTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTTGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTTCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGc                                                                                                                       <  1:1196284/150‑1 (MQ=255)

                               tGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGt                                                                                                                                      <  1:374112/134‑1 (MQ=39)

                               tGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGt                                                                                                                                      >  2:374112/1‑134 (MQ=39)

                               tGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGc‑c                                                                                                                     >  1:2192782/1‑150 (MQ=255)

                                ggTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑Ct                                                                                                                    <  2:165956/150‑1 (MQ=255)

                                 gTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTg                                                                                                                   >  2:1681300/1‑150 (MQ=255)

                                    aTTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGc                                                                                                                >  1:1567108/1‑150 (MQ=255)

                                       tCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCCCGTTGTTGGGC‑CTGGGCtt‑t                                                                                                            <  1:1209960/150‑1 (MQ=255)

                                         cACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTCGCACGCCGCACTAAGCGTCACTCGCCTCTGCAAACGGCCGGCTCCGTTGGGG‑‑ACCCGGCCGTTTAGCGTGGTTAGGG‑CGGGTCgt‑ttt                                                                                                          >  2:1129320/1‑150 (MQ=255)

                                         cACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCtt‑ttt                                                                                                          >  1:1250813/1‑150 (MQ=255)

                                         cACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGAG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCtt‑ttt                                                                                                          >  2:761134/1‑150 (MQ=255)

                                          aCCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑Acc                                                                                                                                             >  1:1979456/1‑116 (MQ=255)

                                             ggTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGc                                                                                                      <  1:237793/150‑1 (MQ=255)

                                              gTATCGCTGCGGCGGTCGTGGGGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCg                                                                                                     <  2:1566545/150‑1 (MQ=255)

                                               tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                     <  1:1410631/119‑1 (MQ=38)

                                               tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                     >  2:1412770/1‑119 (MQ=38)

                                               tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                     <  1:1412770/119‑1 (MQ=38)

                                                   gCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAt                                                                                                >  2:199303/1‑150 (MQ=255)

                                                     tGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAtgt                                                                                              <  1:1811350/150‑1 (MQ=255)

                                                     tGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAtgt                                                                                              <  1:300782/150‑1 (MQ=255)

                                                        ggcggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcca                                                                                                                                 >  1:2445157/1‑112 (MQ=255)

                                                        ggcggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcca                                                                                                                                 <  2:2445157/114‑3 (MQ=255)

                                                        ggcggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CCGGGCTT‑TTTCTGCGGGCATGTGGa                                                                                           <  2:1627447/150‑1 (MQ=255)

                                                           ggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAAc                                                                                        >  1:1034618/1‑150 (MQ=255)

                                                             tCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGc                                                                                      <  2:2178240/150‑1 (MQ=255)

                                                                tggtggCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGTATCGGCCGGCCCCCGTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTgg                                                                                   <  2:314232/150‑1 (MQ=255)

                                                                tggtggCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTgg                                                                                   <  1:2278613/150‑1 (MQ=255)

                                                                  gtggCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGAAAACGCTGGtt                                                                                 >  2:163363/1‑150 (MQ=255)

                                                                          gggTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTTGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACg                                                                         <  1:161264/150‑1 (MQ=255)

                                                                                 gtgCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AAcc                                                                 >  2:1606353/1‑150 (MQ=255)

                                                                                   gCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGCGCCG‑‑CGGCACCATTCGCGCCACCCTCCCGTTTCGCGTGGGTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGa                                                               <  1:2389084/150‑1 (MQ=255)

                                                                                    cACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAg                                                              >  1:85650/1‑150 (MQ=255)

                                                                                     aCCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGc                                                             >  1:765790/1‑150 (MQ=255)

                                                                                      ccAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                            >  1:1186845/1‑150 (MQ=255)

                                                                                      ccAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTG‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                            >  2:2277511/1‑150 (MQ=255)

                                                                                       cAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCg                                                                                                     <  2:1373959/109‑1 (MQ=255)

                                                                                       cAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCg                                                                                                     >  1:1373959/1‑109 (MQ=255)

                                                                                       cAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAg                                                           >  1:749394/1‑150 (MQ=255)

                                                                                        aGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACAGCCGGCTACCCAGAGG‑‑ACCCCCCCGTACCCCGCTGCCCGGT‑CTGCGCTTCGTTCTTCTGTGTTG‑GTAATCTCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                          <  2:2103063/150‑1 (MQ=255)

                                                                                         gATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAggg                                                         >  1:1509762/1‑150 (MQ=255)

                                                                                              gCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                          <  2:104690/144‑1 (MQ=255)

                                                                                              gCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                          >  1:104690/1‑144 (MQ=255)

                                                                                                 ctGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCa                                                 <  1:1255026/150‑1 (MQ=255)

                                                                                                 ctGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGAG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCa                                                 <  1:761134/150‑1 (MQ=255)

                                                                                                    gTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGAGG‑AAGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATAAACGGTG‑AACCGCGCAGGGCTGCCGCAGCg                                              >  1:747394/1‑150 (MQ=255)

                                                                                                     tAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGC‑T‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGAt                                            >  1:628643/1‑150 (MQ=255)

                                                                                                        ctagcagcGCTAGGCGTCGCTCGCCTCTGGAACCGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCa                                          <  2:692119/148‑1 (MQ=255)

                                                                                                        caagcaGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCa                                          <  1:305028/150‑1 (MQ=255)

                                                                                                        caagcaGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGAAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCa                                          <  1:163363/150‑1 (MQ=255)

                                                                                                         aagcaGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGA‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAg                                         >  1:158370/1‑150 (MQ=255)

                                                                                                          agcagcACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAgg                                        <  2:1034618/150‑1 (MQ=255)

                                                                                                          agcagcACTAAGCGTCACTCGCCTCTGCAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAgg                                        <  2:1271535/150‑1 (MQ=255)

                                                                                                           gcagcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAggg                                       >  1:1478211/1‑150 (MQ=255)

                                                                                                            cagcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGc                                      <  1:1129320/150‑1 (MQ=255)

                                                                                                             agcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                          >  2:604287/1‑129 (MQ=255)

                                                                                                             agcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                          <  1:604287/129‑1 (MQ=255)

                                                                                                             agcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGcc                                     >  1:113824/1‑150 (MQ=255)

                                                                                                              gcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCa                                    >  2:173613/1‑150 (MQ=255)

                                                                                                              gcaCTAAGCGTCACTCGACTCTGGAAACGGCCGGCTCCCTTGGGC‑‑AAACCGCCGTTTC‑AATTTTCGCTCGTTGCTCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCa                                    <  1:1224148/150‑1 (MQ=255)

                                                                                                                  tAAGCGTGACTCGCCTCTGTAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGAt                                <  2:1996129/150‑1 (MQ=255)

                                                                                                                  tAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGAt                                >  2:2048820/1‑150 (MQ=255)

                                                                                                                     gCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGANGGc                             <  2:1509762/150‑1 (MQ=255)

                                                                                                                       gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTg                                                                                    >  2:431772/1‑94 (MQ=255)

                                                                                                                       gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTg                                                                                    <  1:431772/94‑1 (MQ=255)

                                                                                                                       gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGa                           <  2:1567108/150‑1 (MQ=255)

                                                                                                                       gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATCTGGAAACGCTGGTGAATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGa                           >  2:1301332/1‑150 (MQ=255)

                                                                                                                         cACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAgg                         <  2:158370/150‑1 (MQ=255)

                                                                                                                         cACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAgg                         <  1:1028111/150‑1 (MQ=255)

                                                                                                                            tCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGGTGGGC‑CTGGGCTG‑TTTCTGCCGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGTATGGCGAGGGCg                      >  2:1326719/1‑150 (MQ=255)

                                                                                                                              gCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGAt                    <  1:395678/150‑1 (MQ=255)

                                                                                                                                 tctGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGC‑T‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAg                <  2:628643/150‑1 (MQ=255)

                                                                                                                                  ctGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAg                >  2:2255262/1‑150 (MQ=255)

                                                                                                                                  ctGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGCCCAGGATGGCGAGGGCGATGTAg                <  2:1186845/150‑1 (MQ=255)

                                                                                                                                   tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                            <  2:821638/105‑1 (MQ=255)

                                                                                                                                   tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGACGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGc                                                                                                      <  1:1192076/64‑1 (MQ=255)

                                                                                                                                   tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGACGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGc                                                                                                      >  2:1192076/1‑64 (MQ=255)

                                                                                                                                    ggAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGt              <  1:173613/150‑1 (MQ=255)

                                                                                                                                     gAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTc             >  2:1999701/1‑150 (MQ=255)

                                                                                                                                        aCGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTc          >  2:301122/1‑150 (MQ=255)

                                                                                                                                           gccggcTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTT‑TTTAATGATTTAACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACg       >  1:1970954/1‑150 (MQ=255)

                                                                                                                                                cTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGt  <  2:747394/150‑1 (MQ=255)

ACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGT  >  NC_002947/2890332‑2890625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`