Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,021,073 A→T 7.0% Q523L (CAG→CTG)  PP_2638 → cellulose synthase operon protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,021,0730AT 7.0% 364.9 / 13.1 116Q523L (CAG→CTG) PP_2638cellulose synthase operon protein C
Reads supporting (aligned to +/- strand):  ref base A (47/60);  new base T (4/4);  total (51/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 119 total aligned reads displayed.

GCGCGAGGACTGGTCGACGGCCAGCCACGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCGTGTTCAGC  >  NC_002947/3020924‑3021222
                                                                                                                                                     |                                                                                                                                                     
gcgcGAGGACTGGTCGACGGCAGGCCTTGCGCGGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTgca                                                                                                                                                       <  1:1817931/150‑1 (MQ=255)
     aGGACTGGTCGACGGCCAGCCACGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGCCTGCGTCACCAAGGGCGCACTGTCTAGGCCTACGCTGCATTTGCCAGGCTGGTGCAGCAcc                                                                                                                                                  <  2:1467578/150‑1 (MQ=255)
      ggACTGGTCGACGGCCCGCCACGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCTGCACCa                                                                                                                                                 <  2:1216877/150‑1 (MQ=255)
       gACTGGTCGACGGCCAGCCACGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAg                                                                                                                                                <  2:441954/150‑1 (MQ=255)
            gTCGACGGCCAGCCACGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCTGCACCAGcctgg                                                                                                                                           <  1:1499156/150‑4 (MQ=255)
                    ccagccaCGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGCGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCgg                                                                                                                                   >  1:745072/1‑150 (MQ=255)
                     cagccaCGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGc                                                                                                                                  >  1:314771/1‑150 (MQ=255)
                         caCGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCaa                                                                                                                                >  2:2006148/1‑148 (MQ=255)
                         caCGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCaa                                                                                                                                <  1:2006148/148‑1 (MQ=255)
                           cGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAAcgcg                                                                                                                            >  2:171342/1‑150 (MQ=255)
                              gCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTa                                                                                                                         >  1:2286655/1‑150 (MQ=255)
                                tGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGt                                                                                                                                          >  1:507266/1‑131 (MQ=255)
                                tGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATg                                                                                                                       >  1:516250/1‑150 (MQ=255)
                                tGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATg                                                                                                                       <  2:1801536/150‑1 (MQ=255)
                                    ccAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCaa                                                                                                                   >  2:2384699/1‑150 (MQ=255)
                                      aGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGATGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACg                                                                                                                 <  1:713136/150‑1 (MQ=255)
                                        gCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGc                                                                                                               <  1:580602/150‑1 (MQ=255)
                                        gCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGc                                                                                                               <  2:745072/150‑1 (MQ=255)
                                        gCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGc                                                                                                               <  1:1149776/150‑1 (MQ=255)
                                        gCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGc                                                                                                               >  1:960629/1‑150 (MQ=255)
                                         cTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGcc                                                                                                              <  2:19282/150‑1 (MQ=255)
                                            cGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCa                                                                                                                                                 <  1:1068588/112‑1 (MQ=255)
                                            cGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCa                                                                                                                                                 >  2:1068588/1‑112 (MQ=255)
                                               cccTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTcc                                                                                                        <  1:2384699/150‑1 (MQ=255)
                                               cccTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTcc                                                                                                        <  1:171342/150‑1 (MQ=255)
                                                ccTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCt                                                                                                       >  2:194840/1‑150 (MQ=255)
                                                 cTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTg                                                                                                      <  2:2445684/150‑1 (MQ=255)
                                                 cTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTg                                                                                                      <  2:417081/150‑1 (MQ=255)
                                                  tGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTgg                                                                                                     >  1:615186/1‑150 (MQ=255)
                                                   gccccccGATGAGCCTTGGCGGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGAAGCGTTACCGGCTACGTGATCCGGCAACGCGCTATGCCAACGGTCTGTTCCTGGa                                                                                                    <  2:927967/148‑1 (MQ=255)
                                                      ccccGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCgg                                                                                                                                   <  2:1801123/116‑1 (MQ=255)
                                                      ccccGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCgg                                                                                                                                   >  1:1801123/1‑116 (MQ=255)
                                                      ccccGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTc                                                                                                 >  2:2210583/1‑150 (MQ=255)
                                                      ccccGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTc                                                                                                 >  2:1617527/1‑150 (MQ=255)
                                                      ccccGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTc                                                                                                 >  1:1018031/1‑150 (MQ=255)
                                                       cccGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTcc                                                                                                >  1:236642/1‑150 (MQ=255)
                                                        ccGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCa                                                                                               <  2:242645/150‑1 (MQ=255)
                                                                ccTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTg                                                                                                      <  1:880650/135‑1 (MQ=255)
                                                                ccTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTg                                                                                                      >  2:880650/1‑135 (MQ=255)
                                                                     gCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGc                                                                                  >  2:2186830/1‑150 (MQ=255)
                                                                       tGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCTGCACCAGcct                                                                                                                                             <  1:601248/89‑2 (MQ=255)
                                                                       tGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCTGCACCAGcct                                                                                                                                             >  2:601248/1‑88 (MQ=255)
                                                                       tGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCt                                                                                >  2:367775/1‑150 (MQ=255)
                                                                       tGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCt                                                                                >  1:1794632/1‑150 (MQ=255)
                                                                        ggTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCtt                                                                               <  2:108956/150‑1 (MQ=255)
                                                                         gtgtATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTg                                                                              <  2:1018031/150‑1 (MQ=255)
                                                                           gtATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGc                                                                            <  2:669047/150‑1 (MQ=255)
                                                                             aTCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGctc                                                                          <  1:777043/150‑1 (MQ=255)
                                                                              tCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGctct                                                                         >  2:2315228/1‑150 (MQ=255)
                                                                                aGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTgg                                                                       <  2:615186/150‑1 (MQ=255)
                                                                                aGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTgg                                                                       >  1:546193/1‑150 (MQ=255)
                                                                                   tGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGaaa                                                                    <  1:2210583/150‑1 (MQ=255)
                                                                                   tGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGaaa                                                                    <  1:1691260/150‑1 (MQ=255)
                                                                                   tGGCCAACAGTCAGCGTACCCCAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGaaa                                                                    <  2:516250/150‑1 (MQ=255)
                                                                                       cAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGctc                                                                          <  1:2109757/140‑1 (MQ=255)
                                                                                       cAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGctc                                                                          >  2:2109757/1‑140 (MQ=255)
                                                                                       cAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCt                                                                >  2:1434544/1‑150 (MQ=255)
                                                                                       cAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCt                                                                >  2:1551304/1‑150 (MQ=255)
                                                                                        aaCAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTg                                                                                                      >  2:1672435/1‑111 (MQ=255)
                                                                                        aaCAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTg                                                               >  2:2189659/1‑150 (MQ=255)
                                                                                        aaCAGTCTGCATCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGAGCGTGATGCCTTGGCTCTGGAAAGTCTg                                                               >  2:1272307/1‑150 (MQ=255)
                                                                                            gTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAggg                                                           <  2:1310458/150‑1 (MQ=255)
                                                                                             tCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGc                                                          <  1:1480089/150‑1 (MQ=255)
                                                                                             tCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGc                                                          >  1:2012368/1‑150 (MQ=255)
                                                                                             tCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGc                                                          >  2:1082077/1‑150 (MQ=255)
                                                                                                  gTCACCAAGGGCGCACTGGCGAGGCCGACGCGTCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTAc                                                     <  1:2051206/150‑1 (MQ=255)
                                                                                                  gTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTTGCTCTGGAAAGTCTGAGGGCGGTAc                                                     >  2:988301/1‑150 (MQ=255)
                                                                                                   tCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCtt                                                                               >  1:267901/1‑123 (MQ=255)
                                                                                                   tCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCtt                                                                               <  2:267901/123‑1 (MQ=255)
                                                                                                   tCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTAcc                                                    >  2:1171629/1‑150 (MQ=255)
                                                                                                     aCCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGc                                                          <  1:1297490/142‑1 (MQ=255)
                                                                                                     aCCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGc                                                          >  2:1297490/1‑142 (MQ=255)
                                                                                                      ccAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGctct                                                                         <  2:780202/126‑1 (MQ=255)
                                                                                                      ccAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCa                                                 >  2:411252/1‑150 (MQ=255)
                                                                                                       cAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAg                                                <  2:886330/150‑1 (MQ=255)
                                                                                                       cAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAg                                                <  1:51447/150‑1 (MQ=255)
                                                                                                           ggCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGa                                            <  1:367775/150‑1 (MQ=255)
                                                                                                            gcgcACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGAc                                           >  2:605607/1‑150 (MQ=255)
                                                                                                                 cTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGa                                      <  2:960629/150‑1 (MQ=255)
                                                                                                                 cTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGa                                      <  2:118600/150‑1 (MQ=255)
                                                                                                                 cTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGa                                      <  1:1082077/150‑1 (MQ=255)
                                                                                                                 cTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGa                                      <  1:2186830/150‑1 (MQ=255)
                                                                                                                    gCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCg                                                         <  2:371614/128‑1 (MQ=255)
                                                                                                                      gAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGcgcg                                 <  2:589024/150‑1 (MQ=255)
                                                                                                                       aGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCgg                                >  2:766344/1‑150 (MQ=255)
                                                                                                                        ggCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGa                               <  2:437160/150‑1 (MQ=255)
                                                                                                                            gACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATg                           <  1:2447353/150‑1 (MQ=255)
                                                                                                                                  gCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACAt                            >  1:819058/1‑143 (MQ=255)
                                                                                                                                   cATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACgcgc                    <  1:1551304/150‑1 (MQ=255)
                                                                                                                                     tttGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGTCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTg                  <  2:2112351/150‑1 (MQ=255)
                                                                                                                                         ccAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTg                                                                        <  1:1204956/92‑1 (MQ=255)
                                                                                                                                          gtggCTGGTGCTGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACAt                            <  1:1620786/133‑1 (MQ=255)
                                                                                                                                          gtggCTGGTGCTGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACAt                            >  2:1620786/3‑135 (MQ=255)
                                                                                                                                          gtggCTGGTGCTGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACa             >  2:650135/3‑150 (MQ=255)
                                                                                                                                            ggCTGGTGCAGCACCAGCCACGTGATCCGACAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGc           <  1:2432205/150‑1 (MQ=255)
                                                                                                                                              cTGGTGCAGCACCAGCCACGTGATCCGGTAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCgt         <  1:935846/150‑1 (MQ=255)
                                                                                                                                               tGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTGCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCgtg        >  1:655155/1‑150 (MQ=255)
                                                                                                                                               tGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCgtg        <  1:2315228/150‑1 (MQ=255)
                                                                                                                                                    cagcaCCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCGTGTTCAg   >  1:2298489/1‑150 (MQ=255)
                                                                                                                                                     agcaCCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCGTGTTCAgc  >  1:1488191/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGCGAGGACTGGTCGACGGCCAGCCACGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCGTGTTCAGC  >  NC_002947/3020924‑3021222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑