Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,077,153 A→C 7.9% I248S (ATC→AGC)  PP_2687 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,077,1530AC 7.9% 257.2 / 12.3 96I248S (ATC→AGC) PP_2687hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (47/41);  new base C (3/5);  total (50/46)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

GTCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCG  >  NC_002947/3077008‑3077299
                                                                                                                                                 |                                                                                                                                                   
gTCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCa                                                                                                                                                 >  1:426236/1‑150 (MQ=255)
gTCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCa                                                                                                                                                 <  2:1057635/150‑1 (MQ=255)
 tCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAg                                                                                                                                                <  1:1544532/150‑1 (MQ=255)
 tCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAg                                                                                                                                                <  1:717253/150‑1 (MQ=255)
 tCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAg                                                                                                                                                <  1:709384/150‑1 (MQ=255)
   gCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGc                                                                                                                                              >  1:697433/1‑150 (MQ=255)
   gCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGc                                                                                                                                              >  2:2005083/1‑150 (MQ=255)
    cTTAGCCAGGGATGCAACCCTGCATCGAGGGAGTGGGCGTGTGCGGGGTGGGGCATGCGGGCGTTCAGGTGGGAGCACCTGCTAGGTCAGTCTCGTCCTGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCa                                                                                                                                             >  1:1751237/1‑150 (MQ=255)
     ttAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCTGTTCCTCGATGCAGGCAt                                                                                                                                            <  1:81694/150‑1 (MQ=255)
     ttAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCAt                                                                                                                                            <  2:2365115/150‑1 (MQ=255)
         ccAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCag                                                                                                                                        >  1:202708/1‑150 (MQ=255)
         ccAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCag                                                                                                                                        >  2:1186889/1‑150 (MQ=255)
          cAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCagc                                                                                                                                       >  1:1673198/1‑150 (MQ=255)
            gggATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCGTGCGCTGGGCGGTACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCagcga                                                                                                                                     <  1:1989655/150‑1 (MQ=255)
             ggATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgag                                                                                                                                    <  1:2297467/150‑1 (MQ=255)
             ggATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGCTATCTTTTGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgag                                                                                                                                    <  2:2433251/150‑1 (MQ=255)
                 gCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGcgcg                                                                                                                                <  1:1699932/150‑1 (MQ=255)
                 gCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGcgcg                                                                                                                                >  1:1079430/1‑150 (MQ=255)
                   aaCCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCTTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgaggaactg                                                                                                                              <  2:100667/150‑7 (MQ=255)
                       cTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcga                                                                                                                                     >  1:1669799/1‑139 (MQ=255)
                       cTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcga                                                                                                                                     <  2:1669799/139‑1 (MQ=255)
                          cagcGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGGGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGACTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCa                                                                                                                      <  1:764705/147‑1 (MQ=255)
                           aTCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCAc                                                                                                                     <  2:1673198/150‑1 (MQ=255)
                                ggATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCa                                                                                                                >  2:1556581/1‑150 (MQ=255)
                                       gtcgATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAAcagcag                                                                                                         <  1:2005083/150‑1 (MQ=255)
                                       gtcgATGGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCCGCCAGGATCAGGTCCAGTTCCTCGATGCCGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAAcagcag                                                                                                         <  2:122710/150‑1 (MQ=255)
                                        tcgATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACagcagc                                                                                                        >  2:252897/1‑150 (MQ=255)
                                        tcgATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACagcagc                                                                                                        >  1:566191/1‑150 (MQ=255)
                                             tGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGTTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTc                                                                                                   <  2:566191/150‑1 (MQ=255)
                                                      gcgcAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCGCGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACAccgg                                                                                          >  1:555591/1‑150 (MQ=255)
                                                          aGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACAccggccgg                                                                                      >  2:1951142/1‑150 (MQ=255)
                                                              gcgcgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATg                                                                                                                                           <  2:744628/94‑1 (MQ=255)
                                                              gcgcgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATg                                                                                                                                           >  1:744628/1‑94 (MQ=255)
                                                                gcgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTg                                                                                <  2:264701/150‑1 (MQ=255)
                                                                 cgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTgg                                                                               <  1:396175/150‑1 (MQ=255)
                                                                 cgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGGCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTgg                                                                               <  2:710451/150‑1 (MQ=255)
                                                                  gTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCGGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTggg                                                                              >  1:1879154/1‑150 (MQ=255)
                                                                  gTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTggg                                                                              >  2:1200447/1‑150 (MQ=255)
                                                                   ttCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGt                                                                             >  2:1652528/1‑150 (MQ=255)
                                                                   ttCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGt                                                                             >  2:2447998/1‑150 (MQ=255)
                                                                          cggcggCCATGCGCTGGGCGGCACGCCCGGTACTGCCCCGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGgcgc                                                                      <  1:623780/150‑1 (MQ=255)
                                                                            gcggcCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGa                                                                    <  1:2447998/150‑1 (MQ=255)
                                                                              ggcCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATa                                                                  <  2:955664/150‑1 (MQ=255)
                                                                              ggcCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATa                                                                  <  1:252897/150‑1 (MQ=255)
                                                                                     cgcTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgagga                                                                                                                                  <  1:684706/80‑3 (MQ=37)
                                                                                     cgcTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgagga                                                                                                                                  >  2:684706/1‑78 (MQ=37)
                                                                                     cgcTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCg                                                           <  2:1089615/150‑1 (MQ=255)
                                                                                       cTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGtt                                                         <  2:697433/150‑1 (MQ=255)
                                                                                       cTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGtt                                                         <  2:1068069/150‑1 (MQ=255)
                                                                                        tGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTgcg                                                                          >  2:288263/1‑132 (MQ=255)
                                                                                        tGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCATCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTgcg                                                                          <  1:288263/132‑1 (MQ=255)
                                                                                          ggcggcACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGaa                                                      <  2:1934768/150‑1 (MQ=255)
                                                                                              gcACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCt                                                  <  2:884071/150‑1 (MQ=255)
                                                                                                aCGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTCCAGTCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTc                                                >  1:2132501/1‑150 (MQ=255)
                                                                                                 cGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTACAGTTCCTCGATGCAAGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCg                                               >  2:1044467/1‑150 (MQ=255)
                                                                                                  gCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGt                                              <  1:1556581/150‑1 (MQ=255)
                                                                                                  gCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGt                                              >  2:280729/1‑150 (MQ=255)
                                                                                                      ggTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGa                                          <  1:2234267/150‑1 (MQ=255)
                                                                                                      ggTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGa                                          <  2:426236/150‑1 (MQ=255)
                                                                                                          cTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTc                                      >  2:1532281/1‑150 (MQ=255)
                                                                                                           tGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCa                                     >  2:2387305/1‑150 (MQ=255)
                                                                                                           tGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGACCGCGG‑CACGTCCACCCGCAACAACAGCAACTCGGACACCGGCCGGGCCCTGGGTGCGGCGCGATAGATCTCGGTGAAGGCTTCGTACgctggcc                                     >  1:531451/1‑144 (MQ=255)
                                                                                                             cctggCAGGCCTGCCGGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGAAACCACACGGCTAACGTCCGGTCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAgc                                   >  1:281366/4‑150 (MQ=255)
                                                                                                              ccGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAgcg                                  <  1:1094107/150‑1 (MQ=255)
                                                                                                                       cTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCgcagca                         >  2:1078963/1‑150 (MQ=255)
                                                                                                                        tGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGcagcag                        >  1:227337/1‑150 (MQ=255)
                                                                                                                         gCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAg                                         >  1:2216704/1‑132 (MQ=255)
                                                                                                                         gCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAg                                         <  2:2216704/132‑1 (MQ=255)
                                                                                                                         gCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAgg                       >  2:865/1‑150 (MQ=255)
                                                                                                                         gCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAgg                       <  1:475602/150‑1 (MQ=255)
                                                                                                                          ccAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAggg                      >  2:691606/1‑150 (MQ=255)
                                                                                                                           cAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCACGCGCAGCAGGgc                     >  2:1292255/1‑150 (MQ=255)
                                                                                                                              gATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGa                                                       <  2:1413284/113‑1 (MQ=255)
                                                                                                                              gATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGa                                                       >  1:1413284/1‑113 (MQ=255)
                                                                                                                                   ggTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCAtt             >  1:345885/1‑150 (MQ=255)
                                                                                                                                    gTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATCTTGCACCTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTg            <  1:1995672/150‑1 (MQ=255)
                                                                                                                                    gTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTg            <  1:1652528/150‑1 (MQ=255)
                                                                                                                                    gTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTg            >  1:1599375/1‑150 (MQ=255)
                                                                                                                                     tCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTgg           >  2:1137901/1‑150 (MQ=255)
                                                                                                                                      ccAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGt          <  1:824340/150‑1 (MQ=255)
                                                                                                                                         gTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGtaat       <  1:1044467/150‑1 (MQ=255)
                                                                                                                                          ttCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGtt                                                         >  1:1541478/1‑99 (MQ=255)
                                                                                                                                          ttCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGtt                                                         <  2:1541478/99‑1 (MQ=255)
                                                                                                                                          ttCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGgc                     <  1:2011297/135‑1 (MQ=255)
                                                                                                                                          ttCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGgc                     >  2:2011297/1‑135 (MQ=255)
                                                                                                                                             cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTGCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCg                                               >  2:1345386/1‑106 (MQ=255)
                                                                                                                                             cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCg                                               <  1:1345386/106‑1 (MQ=255)
                                                                                                                                             cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc   >  2:526770/1‑150 (MQ=255)
                                                                                                                                             cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc   >  2:388124/1‑150 (MQ=255)
                                                                                                                                             cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc   >  2:316069/1‑150 (MQ=255)
                                                                                                                                             cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc   >  1:778709/1‑150 (MQ=255)
                                                                                                                                             cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc   >  2:889079/1‑150 (MQ=255)
                                                                                                                                             cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTAAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc   >  2:1694838/1‑150 (MQ=255)
                                                                                                                                              tCGATGTAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACcg  >  2:577855/1‑150 (MQ=255)
                                                                                                                                              tCGATGCAGGCATGCAGCGAGCGCGGACAGAACCA‑CCGCAACAGCAGCACCTTAGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACcg  >  1:299870/1‑150 (MQ=255)
                                                                                                                                              tCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGTATCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACcg  >  1:2399433/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCG  >  NC_002947/3077008‑3077299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑