Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,116,213 T→C 6.9% L303P (CTG→CCG)  PP_2732 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,116,2130TC 6.9% 259.6 / 6.5 87L303P (CTG→CCG) PP_2732hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (42/39);  new base C (2/4);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

CGAAGGCGAGCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATCG  >  NC_002947/3116064‑3116359
                                                                                                                                                     |                                                                                                                                                  
cGAAGGCGAGCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGACGGCTTCATTCGCCAGCt                                                                                                                                                    >  2:137554/1‑150 (MQ=255)
 gAAGGCGAGCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTg                                                                                                                                                   >  1:17424/1‑150 (MQ=255)
        aGCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCt                                                                                                                                            <  2:748335/150‑1 (MQ=255)
        aGCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCt                                                                                                                                            >  2:1769876/1‑150 (MQ=255)
         gCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTg                                                                                                                                           <  2:308430/150‑1 (MQ=255)
           cGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGc                                                                                                                                         <  1:553483/150‑1 (MQ=255)
             tACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGt                                                                                                                                       <  1:38854/150‑1 (MQ=255)
             tACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGt                                                                                                                                       <  1:696412/150‑1 (MQ=255)
             tACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGt                                                                                                                                       >  1:1827273/1‑150 (MQ=255)
             tACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGt                                                                                                                                       <  2:1827273/150‑1 (MQ=255)
             tACCTGTTTCATGCGCGCATCAGTGCGACGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGt                                                                                                                                       <  2:1268544/150‑1 (MQ=255)
              aCCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTg                                                                                                                                      <  1:1687663/150‑1 (MQ=255)
              aCCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATCAGCTGGCgaa                                                                                                                                      <  2:1345746/150‑3 (MQ=255)
               ccTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGa                                                                                                                                     >  1:2287814/1‑150 (MQ=255)
                cTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAg                                                                                                                                    >  1:31674/1‑150 (MQ=255)
                 tGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGt                                                                                                                                   >  2:1111870/1‑150 (MQ=255)
                  gTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATCAGCTGGCgaatgaa                                                                                                                                  <  2:380237/150‑7 (MQ=255)
                   tttCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAc                                                                                                                                 >  1:1503347/1‑150 (MQ=255)
                   tttCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAc                                                                                                                                 <  2:978649/150‑1 (MQ=255)
                     tCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGACGCGCTTTCCCTACCCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAcgt                                                                                                                               <  2:1880787/150‑1 (MQ=255)
                       aTGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAcgtgc                                                                                                                             >  1:796660/1‑150 (MQ=255)
                        tGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGTCGTGAGTAcgtgcg                                                                                                                            >  1:2063561/1‑150 (MQ=255)
                        tGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAcgtgcg                                                                                                                            >  1:2028907/1‑150 (MQ=255)
                          cgcgcATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAcgtgcgtg                                                                                                                          <  1:1769876/150‑1 (MQ=255)
                          cgcgcATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAcgtgcgtg                                                                                                                          <  2:2028907/150‑1 (MQ=255)
                             gcATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCg                                                                                                                                               <  2:1173384/126‑1 (MQ=255)
                             gcATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCg                                                                                                                                               >  1:1173384/1‑126 (MQ=255)
                             gcATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCa                                                                                                                       <  2:989319/150‑1 (MQ=255)
                                   gTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTAtt                                                                                                                 >  1:892146/1‑150 (MQ=255)
                                    tGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTg                                                                                                                >  2:602903/1‑150 (MQ=255)
                                     gcggcgCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTgg                                                                                                               <  1:1979097/150‑1 (MQ=255)
                                      cggcgCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGc                                                                                                              >  1:977994/1‑150 (MQ=255)
                                       ggcgCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCa                                                                                                             <  2:1000261/150‑1 (MQ=255)
                                       ggcgCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCa                                                                                                             <  1:1788804/150‑1 (MQ=255)
                                       ggcgCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCa                                                                                                             >  1:400891/1‑150 (MQ=255)
                                           cTCAACATCGGCCTTCTGGGTGTGCGCCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAgcgc                                                                                                         >  1:1091847/1‑150 (MQ=255)
                                            tCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCa                                                                                                        >  2:2253873/1‑150 (MQ=255)
                                              aaCATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATg                                                                                                      >  1:7897/1‑150 (MQ=255)
                                                cATCGGCCTTCTGGATGTGCGTCGTCTGTGCGGCGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGcc                                                                                                    >  1:1866527/1‑150 (MQ=255)
                                                 aTCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCg                                                                                                   <  1:927498/150‑1 (MQ=255)
                                                 aTCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCg                                                                                                   <  2:7897/150‑1 (MQ=255)
                                                  tCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCgg                                                                                                  <  2:400891/150‑1 (MQ=255)
                                                     gCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGTAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAcgt                                                                                                                               <  2:2311052/118‑1 (MQ=255)
                                                     gCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGTAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAcgt                                                                                                                               >  1:2311052/1‑118 (MQ=255)
                                                        ttCTGGATGTGAGTCGCATATGCGTCTGCGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATg                                                                                            >  2:1402083/1‑150 (MQ=255)
                                                            ggATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGTGCATGCCGGCATATGCCgg                                                                                        <  1:2172390/150‑1 (MQ=255)
                                                             gATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCggg                                                                                       <  2:1702181/150‑1 (MQ=255)
                                                             gATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCggg                                                                                       >  2:1659211/1‑150 (MQ=255)
                                                             gATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATc                                                                                                                                                <  2:2326449/93‑1 (MQ=255)
                                                             gATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATc                                                                                                                                                >  1:2326449/1‑93 (MQ=255)
                                                              aTGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGc                                                                                      >  1:318674/1‑150 (MQ=255)
                                                                gtgCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTc                                                                                    >  1:1822454/1‑150 (MQ=255)
                                                                  gCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCa                                                                                                <  1:890171/136‑1 (MQ=255)
                                                                  gCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCa                                                                                                >  2:890171/1‑136 (MQ=255)
                                                                  gCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATCAGCTGGCgaat                                                                                                                                     <  1:1492586/99‑4 (MQ=255)
                                                                  gCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATCAGCTGGCgaat                                                                                                                                     >  2:1492586/1‑96 (MQ=255)
                                                                   cgtcgtCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAAt                                                                                 >  1:1155572/1‑150 (MQ=255)
                                                                     gcgtcTGGTCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCa                                                                               <  1:1254646/149‑1 (MQ=255)
                                                                       gtcTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCAcc                                                                             <  1:784601/150‑1 (MQ=255)
                                                                        tcTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCt                                                                            <  1:1056903/150‑1 (MQ=255)
                                                                                acgacgTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACGATc                                                                    <  1:1111870/150‑1 (MQ=255)
                                                                                acgacgTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATc                                                                    <  2:796660/150‑1 (MQ=255)
                                                                                  gacgTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCAc                                                                  >  1:1467212/1‑150 (MQ=255)
                                                                                   acgTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCAcc                                                                 <  1:1659211/150‑1 (MQ=255)
                                                                                     gTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCgg                                                               >  1:1685565/1‑150 (MQ=255)
                                                                                     gTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTAATTCGTCAGCTGATCGGCTAGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCgg                                                               >  1:292701/1‑150 (MQ=255)
                                                                                        gACAAAGCCTGGCGCGAGGGGCGGGTGACGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGgcg                                                            >  1:1228757/1‑150 (MQ=255)
                                                                                              gCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCa                                                      >  2:412840/1‑150 (MQ=255)
                                                                                                cTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGa                                                    >  2:330141/1‑150 (MQ=255)
                                                                                                      cgAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTa                                              <  2:892146/150‑1 (MQ=255)
                                                                                                      cgAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTa                                              <  2:31674/150‑1 (MQ=255)
                                                                                                        aGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACt                                            <  1:363154/150‑1 (MQ=255)
                                                                                                          gggCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTgg                                          >  2:233975/1‑150 (MQ=255)
                                                                                                          gggCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCAACGGGCGCTGCCAGAATTCTACTgg                                          >  1:1770072/1‑150 (MQ=255)
                                                                                                                                ccGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCt                    >  2:1598150/1‑150 (MQ=255)
                                                                                                                                ccGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCt                    <  1:1402083/150‑1 (MQ=255)
                                                                                                                                   aaGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCa                 <  2:2287814/150‑1 (MQ=255)
                                                                                                                                       cTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGgc             <  2:2223067/150‑1 (MQ=255)
                                                                                                                                        ttCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGgcg            >  2:242763/1‑150 (MQ=255)
                                                                                                                                        ttCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGgcg            >  1:551733/1‑150 (MQ=255)
                                                                                                                                            ttCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAg        >  1:2161664/1‑150 (MQ=255)
                                                                                                                                            ttCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAg        >  1:2164389/1‑150 (MQ=255)
                                                                                                                                                ccAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCAt    >  2:1683553/1‑150 (MQ=255)
                                                                                                                                                 cAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATc   <  1:242763/150‑1 (MQ=255)
                                                                                                                                                 cAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATc   <  1:233975/150‑1 (MQ=255)
                                                                                                                                                  aGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATCg  >  2:1346318/1‑150 (MQ=255)
                                                                                                                                                    cTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCAt                         >  1:1793923/1‑125 (MQ=255)
                                                                                                                                                    cTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCAt                         <  2:1793923/125‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGAAGGCGAGCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATCG  >  NC_002947/3116064‑3116359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑