Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,153,760 G→A 6.0% P326P (CCG→CCA)  PP_2769 → branched‑chain amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,153,7600GA 6.0% 196.3 / 2.5 67P326P (CCG→CCA) PP_2769branched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (33/30);  new base A (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TGCCGACTGGGCTGATCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACG  >  NC_002947/3153615‑3153906
                                                                                                                                                 |                                                                                                                                                  
tgcCTACGGGGCTGATCGATGCCGGGCATATAGAGACCCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCCATGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTgc                                                                                                                                                <  1:181988/150‑1 (MQ=255)
  ccGACTGGGCTGATCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTgcgc                                                                                                                                              <  2:1287239/150‑1 (MQ=255)
          gCTGATCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGc                                                                                                                                      >  1:356888/1‑150 (MQ=255)
             gATCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGaa                                                                                                                                   >  2:1851810/1‑150 (MQ=255)
             gATCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGaa                                                                                                                                   >  1:1480432/1‑150 (MQ=255)
               tCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAAcc                                                                                                                                 >  1:260424/1‑150 (MQ=255)
                   tGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCg                                                                                                                             <  1:67685/150‑1 (MQ=255)
                       gggCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCAAGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCAcc                                                                                                                         >  1:962958/1‑150 (MQ=255)
                          cAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCggg                                                                                                                      >  1:456109/1‑150 (MQ=255)
                           aGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGc                                                                                                                     >  2:1685912/1‑150 (MQ=255)
                             aTAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCtt                                                                                                                   >  1:2421044/1‑150 (MQ=255)
                              tAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGATCCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTc                                                                                                                  <  1:1209322/150‑1 (MQ=255)
                                  gaACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTcgccc                                                                                                              >  1:2072109/1‑150 (MQ=255)
                                     ccTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCATTGCGCTACTGAGCGAACCTTCGCACCGGGCTTcgcccgcc                                                                                                           >  1:1571282/1‑150 (MQ=255)
                                      cTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCt                                                                                                          >  2:105021/1‑150 (MQ=255)
                                         cAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTCCGCTTCTGAGCGGATCTTCGCACCGGGCTTCGCACGGCttct                                                                                                       >  2:391524/1‑148 (MQ=255)
                                           gaagaTTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGc                                                                                                     >  2:608935/1‑150 (MQ=255)
                                           gaagaTTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGc                                                                                                     <  2:108992/150‑1 (MQ=255)
                                               aTTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACt                                                                                                 >  1:2139571/1‑150 (MQ=255)
                                                    cTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCa                                                                                            >  2:988965/1‑150 (MQ=255)
                                                    cTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCa                                                                                            >  2:492531/1‑150 (MQ=255)
                                                     ttCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCAc                                                                                           >  2:1179663/1‑150 (MQ=255)
                                                     ttCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCAc                                                                                           >  2:2451407/1‑150 (MQ=255)
                                                     ttCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCAc                                                                                           >  1:1388209/1‑150 (MQ=255)
                                                     ttCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCAc                                                                                           >  2:2452738/1‑150 (MQ=255)
                                                      tCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACt                                                                                          <  1:608935/150‑1 (MQ=255)
                                                         gCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGAt                                                                                       <  1:2451407/150‑1 (MQ=255)
                                                         gCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGAt                                                                                       <  1:2452738/150‑1 (MQ=255)
                                                         gCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGAt                                                                                       <  1:1179663/150‑1 (MQ=255)
                                                         gCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGAt                                                                                       <  1:1851810/150‑1 (MQ=255)
                                                         gCACGCTGATCATCATCTTCCTGATCAAGGAACCGCAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGAt                                                                                       <  2:1685981/150‑1 (MQ=255)
                                                             gCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCATTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGt                                                                                   >  2:1556027/1‑150 (MQ=255)
                                                              cTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTa                                                                                  >  2:858668/1‑150 (MQ=255)
                                                                 atcatcatcTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAg                                                                               <  2:934580/150‑1 (MQ=255)
                                                                  tcatcatcTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAgg                                                                              >  2:1820387/1‑150 (MQ=255)
                                                                       atcTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAAc                                                                         <  1:105021/150‑1 (MQ=255)
                                                                       atcTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAAc                                                                         <  1:492531/150‑1 (MQ=255)
                                                                           tCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACg                                                                     >  1:218462/1‑150 (MQ=255)
                                                                             cTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGGGTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGAt                                                                   >  1:745038/1‑150 (MQ=255)
                                                                              tGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGa                                                                                                                                        >  2:2363320/1‑80 (MQ=255)
                                                                              tGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGa                                                                                                                                        <  1:2363320/80‑1 (MQ=255)
                                                                                aTCAAGGAACCGGAGGGCCTGGTGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTaaa                                                                                >  2:30240/1‑134 (MQ=255)
                                                                                aTCAAGGAACCGGAGGGCCTGGTGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTaaa                                                                                <  1:30240/134‑1 (MQ=255)
                                                                                   aaGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGAc                                                             <  1:1685912/150‑1 (MQ=255)
                                                                                    aGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGAcc                                                            <  2:962958/150‑1 (MQ=255)
                                                                                    aGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGAcc                                                            >  1:928719/1‑150 (MQ=255)
                                                                                        aCCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAg                                                                               <  1:1173198/127‑1 (MQ=255)
                                                                                        aCCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAg                                                                               >  2:1173198/1‑127 (MQ=255)
                                                                                          cGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATcc                                                      >  1:1113927/1‑150 (MQ=255)
                                                                                          cGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATcc                                                      <  2:543008/150‑1 (MQ=255)
                                                                                                 ccTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCg                                               <  1:1820387/150‑1 (MQ=255)
                                                                                                 ccTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCg                                               <  2:260424/150‑1 (MQ=255)
                                                                                                          ccTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTgg                                      <  2:1631678/150‑1 (MQ=255)
                                                                                                             ctGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGgcgc                                   <  2:1113927/150‑1 (MQ=255)
                                                                                                                gCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTgg                                >  2:1487455/1‑150 (MQ=255)
                                                                                                                  aaCGCTTTCGCGAGCGCGCTCGGGGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCTCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGcc                              >  2:1192781/1‑150 (MQ=255)
                                                                                                                   aCGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCg                             >  1:1375120/1‑150 (MQ=255)
                                                                                                                       tttCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAg                         <  1:1769459/150‑1 (MQ=255)
                                                                                                                        ttCGCGAGCGCGCTCGGTGTTGGTCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGc                        <  1:398309/150‑1 (MQ=255)
                                                                                                                            cgAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAg                    <  1:858668/150‑1 (MQ=255)
                                                                                                                              aGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCt                  <  2:1480432/150‑1 (MQ=255)
                                                                                                                              aGCGCGCTCGGCGTTGGCCATTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCt                  <  2:1571282/150‑1 (MQ=255)
                                                                                                                                    cTCGGCGTTGGCCATTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGgc            <  1:1556027/150‑1 (MQ=255)
                                                                                                                                       ggCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAAc                                                                         <  1:1411413/86‑1 (MQ=255)
                                                                                                                                       ggCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCa         >  2:2129569/1‑150 (MQ=255)
                                                                                                                                       ggCGTTGGCCGTTGCGCTACTGAGCGAACCATCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAAc                                                                         >  2:1411413/1‑86 (MQ=255)
                                                                                                                                              gCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACg  <  2:218462/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TGCCGACTGGGCTGATCGATGCCGGGCAGATAGAGAACCTGCAGAAGATTGCCTTCGGCACGCTGATCATCATCTTCCTGATCAAGGAACCGGAGGGCCTGGCGCGCCTCTGGCAACGCTTTCGCGAGCGCGCTCGGCGTTGGCCGTTGCGCTACTGAGCGAACCTTCGCACCGGGCTTCGCCCGCCTTATGCCACTGCCCACTGATTTGTAAAGGATAACCACGATGTCGACCCGATCCGTCCTCGGCCGCCTGGCGCTGGCCGTAAGCTTAGCTGCCGGCGCACTCAACG  >  NC_002947/3153615‑3153906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑