BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,228,341	C→T	6.5%	H379H (CAC→CAT) ‡	PP_2828 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,228,341	0	C	T	6.5%	365.6 / 10.7	123	H379H (CAC→CAT) ‡	PP_2828	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (57/58); new base T (3/5); total (60/63)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 123 total aligned reads displayed.

GCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCAGTTGCCGTTGGCGG  >  NC_002947/3228194‑3228489

gcaggCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGc                                                                                                                                                    <  1:2411014/150‑1 (MQ=255)

gcaggCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGc                                                                                                                                                    <  1:1322464/150‑1 (MQ=255)

       ggTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCGTGCCGACact                                                                                                                                             <  1:321990/150‑3 (MQ=255)

        gTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTACAGCGGTTCTTGCAGTGGCGTCGCGCCGACAggg                                                                                                                                            >  1:164762/1‑150 (MQ=255)

           gcagcaAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACAtggtgg                                                                                                                                         >  2:908238/1‑150 (MQ=255)

            cagcaAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACAtggtggt                                                                                                                                        <  2:444115/150‑1 (MQ=255)

              gcaagcaGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTAAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACAtggtggtgg                                                                                                                                      >  1:1860139/1‑150 (MQ=255)

               caagcaGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCGGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGc                                                                                                                                     >  1:1001084/1‑150 (MQ=255)

               caagcaGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTATGTTTGTTTGCCAGGCAGGGGAAAAACCCCCCGCCGCCCTCGCAGTGTCGCCACGCCCACATGGCGGTGGc                                                                                                                                     >  1:1001450/1‑150 (MQ=255)

                   caccGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGGGTGCCTGCTGTTCAGCCCGGTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTTGCGTTg                                                                                                                                 >  1:422607/4‑150 (MQ=255)

                    agcGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGc                                                                                                                                <  1:1538973/150‑1 (MQ=255)

                     gcGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCTGGCCAAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAACACCGCCAGTGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGcc                                                                                                                               <  2:420203/150‑1 (MQ=255)

                     gcGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGcc                                                                                                                               >  1:496073/1‑150 (MQ=255)

                         gACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTAc                                                                                                                           <  2:1483177/150‑1 (MQ=255)

                            gccgtgccgtTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTg                                                                                                                        <  1:2011492/150‑1 (MQ=255)

                            gccgtgccgtTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTg                                                                                                                        >  1:1973940/1‑150 (MQ=255)

                              cgtgccgtTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGca                                                                                                                      >  2:2407386/1‑150 (MQ=255)

                                 gccgtTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAg                                                                                                                   <  1:386540/150‑1 (MQ=255)

                                    gtTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCGTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGc                                                                                                                >  2:322428/1‑150 (MQ=255)

                                    gtTGCTATTTGGCAAAGCCCACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGc                                                                                                                >  1:875625/1‑150 (MQ=255)

                                     ttGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACGGGGcc                                                                                                               >  2:79911/1‑150 (MQ=255)

                                      tGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCt                                                                                                              <  1:1781496/150‑1 (MQ=255)

                                       gCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCtt                                                                                                             <  2:1973940/150‑1 (MQ=255)

                                       gCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCtt                                                                                                             <  1:2435205/150‑1 (MQ=255)

                                         tATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGcc                                                                                                               <  2:1296474/146‑1 (MQ=255)

                                         tATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGcc                                                                                                               >  1:1296474/1‑146 (MQ=255)

                                           tttGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCaa                                                                                                         >  2:200453/1‑150 (MQ=255)

                                               gCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCa                                                                                                     >  1:2351154/1‑150 (MQ=255)

                                                cAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCaa                                                                                                    >  1:225462/1‑150 (MQ=255)

                                                 aaaGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCaaa                                                                                                   >  1:1087556/1‑150 (MQ=255)

                                                 aaaGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCaaa                                                                                                   <  2:496073/150‑1 (MQ=255)

                                                 aaaGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCaaa                                                                                                   >  1:264225/1‑150 (MQ=255)

                                                       tACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAgg                                                                                                       <  2:228488/140‑1 (MQ=255)

                                                         cGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGcaca                                                                                                                    <  2:1885113/125‑1 (MQ=255)

                                                         cGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGcaca                                                                                                                    >  1:1885113/1‑125 (MQ=255)

                                                         cGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTg                                                                                              <  1:2383384/147‑1 (MQ=255)

                                                         cGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTg                                                                                              >  2:2383384/1‑147 (MQ=255)

                                                             aTCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCATGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACCTGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGAt                                                                                       <  2:1257610/150‑1 (MQ=255)

                                                                      gCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCaaa                                                                              >  1:1152077/1‑150 (MQ=255)

                                                                        ccAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGc                                                                            <  1:604331/150‑1 (MQ=255)

                                                                         cAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGcc                                                                           <  2:225462/150‑1 (MQ=255)

                                                                         cAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTCCCCCGATGCCGTCAAAccc                                                                           >  2:528158/1‑150 (MQ=255)

                                                                          aaCTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACAtggtg                                                                                                                                          <  2:2196877/86‑1 (MQ=255)

                                                                          aaCTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACAtggtg                                                                                                                                          >  1:2196877/1‑86 (MQ=255)

                                                                          aaCTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACAtggtg                                                                                                                                          >  1:950995/1‑86 (MQ=255)

                                                                          aaCTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCct                                                                          <  2:1001450/150‑1 (MQ=255)

                                                                          aaCTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCct                                                                          <  2:1001084/150‑1 (MQ=255)

                                                                               gtgtGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCACGGCAAACCCTGCCCCGATGCAGTCAAAGCctggctg                                                                     >  1:526930/1‑150 (MQ=255)

                                                                               gtgtGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCctggctg                                                                     >  1:103640/1‑150 (MQ=255)

                                                                                   gCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGa                                                                 >  1:1371819/1‑150 (MQ=255)

                                                                                    ccTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGaa                                                                <  2:420206/150‑1 (MQ=255)

                                                                                    ccTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGaa                                                                <  1:908238/150‑1 (MQ=255)

                                                                                     ctgctgTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGaa                                                                <  1:1664749/149‑1 (MQ=255)

                                                                                     ctgctgTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGaa                                                                >  2:1664749/1‑149 (MQ=255)

                                                                                      tgctgTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACGGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATa                                                              <  1:79911/150‑1 (MQ=255)

                                                                                      tgctgTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATa                                                              >  2:717223/1‑150 (MQ=255)

                                                                                      tgctgTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATa                                                              >  2:881109/1‑150 (MQ=255)

                                                                                       gctgTTCAGCCCGTTTGCCAGGCAGGTGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATAc                                                             <  2:912375/150‑1 (MQ=255)

                                                                                       gctgTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGcc                                                                                                                               <  1:554296/84‑1 (MQ=255)

                                                                                       gctgTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGcc                                                                                                                               >  2:554296/1‑84 (MQ=255)

                                                                                           ttCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGTAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGt                                                         >  1:726154/1‑150 (MQ=255)

                                                                                           ttCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGt                                                         >  1:1556800/1‑150 (MQ=255)

                                                                                           ttCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGt                                                         >  1:352830/1‑150 (MQ=255)

                                                                                                 ccGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTg                                                   >  1:1526399/1‑150 (MQ=255)

                                                                                                   gTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGcc                                                 >  1:515890/1‑150 (MQ=255)

                                                                                                       gCCAGGCAGGGGAAAAACCTCGGGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTg                                             <  1:342612/150‑1 (MQ=255)

                                                                                                        ccAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTgg                                            <  1:881109/150‑1 (MQ=255)

                                                                                                        ccAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTgg                                            <  1:200453/150‑1 (MQ=255)

                                                                                                            gcaggGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGt                                                                   >  1:581719/1‑123 (MQ=255)

                                                                                                                 ggAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGc                                   <  1:2383437/150‑1 (MQ=255)

                                                                                                                 ggAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGc                                   <  1:2249128/150‑1 (MQ=255)

                                                                                                                    aaaaCCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGc                                >  1:778493/1‑150 (MQ=255)

                                                                                                                     aaaCCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCa                               >  2:2211037/1‑150 (MQ=255)

                                                                                                                       aCCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCaaa                             <  1:1558886/150‑1 (MQ=255)

                                                                                                                         cTCGAGCGGTTCTTGCAGTGTCGTCACGCCGACATGGTAGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCACCT‑‑GGCAAATTTa                         >  2:495437/1‑150 (MQ=255)

                                                                                                                         cTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGtt                                              <  2:1404332/131‑1 (MQ=255)

                                                                                                                         cTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGtt                                              >  1:1404332/1‑131 (MQ=255)

                                                                                                                         cTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAAtt                           >  1:2425066/1‑150 (MQ=255)

                                                                                                                           cGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTa                         <  2:1152077/150‑1 (MQ=255)

                                                                                                                            gAGCGGTTCTTGTAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTat                        <  2:726154/150‑1 (MQ=255)

                                                                                                                            gAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTat                        >  1:1848452/1‑150 (MQ=255)

                                                                                                                            gAGCGGTTCTTGCAGTGGCGGCACGCCGACATGGTGGTGGCGGTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGCTGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGTCGTTGGGGCAACTGCGGCAAATTTat                        <  1:514058/150‑1 (MQ=255)

                                                                                                                             aGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTatg                       >  1:800540/1‑150 (MQ=255)

                                                                                                                               cGGTTTTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTatgat                     <  2:1169479/150‑1 (MQ=255)

                                                                                                                                ggTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTatgatg                    <  1:2234089/150‑1 (MQ=255)

                                                                                                                                  ttCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATggg                  >  1:2383658/1‑150 (MQ=255)

                                                                                                                                     ttGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGATGCGGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCAg               <  1:1842820/150‑1 (MQ=255)

                                                                                                                                       gCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCAGtt             <  2:1087556/150‑1 (MQ=255)

                                                                                                                                         catgtCGGCGTGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTg                                                                  <  1:1980356/93‑1 (MQ=255)

                                                                                                                                         catgtCGGCGTGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTg                                                                  >  2:1980356/3‑95 (MQ=255)

                                                                                                                                          gtgtCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTgg                                            >  1:155852/1‑116 (MQ=255)

                                                                                                                                          gtgtCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCAGTTGcc          <  2:1860139/150‑1 (MQ=255)

                                                                                                                                          atgtCGGCGTGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCa                <  2:618246/143‑1 (MQ=255)

                                                                                                                                          atgtCGGCGTGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCa                <  2:618266/143‑1 (MQ=255)

                                                                                                                                          atgtCGGCGTGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCa                >  1:618246/2‑144 (MQ=255)

                                                                                                                                          atgtCGGCGTGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCa                >  1:618266/2‑144 (MQ=255)

                                                                                                                                               ggCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTgg                                                       <  2:491356/100‑1 (MQ=255)

                                                                                                                                                  aCGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGt                                                         >  2:1621393/1‑95 (MQ=255)

                                                                                                                                                  aCGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGt                                                         <  1:1621393/95‑1 (MQ=255)

                                                                                                                                                  aCGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCAGTTGCCGTTGGCgg  <  1:2279419/150‑1 (MQ=255)

GCAGGCGGGTGGCAGCAAGCAGCGTGACGCCGTGCCGTTGCTATTTGGCAAAGCCTACGGCATCAACCAGGCCCAACTGGTGTGCCTGCTGTTCAGCCCGTTTGCCAGGCAGGGGAAAAACCTCGAGCGGTTCTTGCAGTGTCGGCACGCCGACATGGTGGTGGCGTTGCCGTACCTGCACAGGGCCTTGCAAGGCAAACCCTGCCCCGATGCAGTCAAAGCCTGGCTGGTGAATACCAGTGGCTTGCCGTTGGGGCAACTGCGGCAAATTTATGATGGGCAGTTGCCGTTGGCGG  >  NC_002947/3228194‑3228489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`