Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,808,2260AG50.0% ‑4.5 / 36.4 30intergenic (‑290/‑265)PP_4234/dsbD‑IIbifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
Reads supporting (aligned to +/- strand):  ref base A (9/5);  new base G (5/9);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCT  >  NC_002947/4808083‑4808375
                                                                                                                                               |                                                                                                                                                     
aTGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgctt                                                                                                                                                 >  1:1268233/1‑148 (MQ=255)
   ggCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGCGAGTATCAGCGCCGCGGAGAACGAGCGCCGCCCGCGCGgcgcttcgc                                                                                                                                              >  2:4692589/1‑145 (MQ=37)
   ggCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgcttcgc                                                                                                                                              <  2:59046/150‑6 (MQ=255)
   ggCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgcttcgc                                                                                                                                              <  2:3344845/150‑6 (MQ=255)
   ggCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgcttcgc                                                                                                                                              <  1:626889/150‑6 (MQ=255)
    gCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg                                                                                                                                             >  2:3739860/1‑150 (MQ=255)
    gCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg                                                                                                                                             <  2:4256314/150‑1 (MQ=255)
    gCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg                                                                                                                                             <  2:309760/150‑1 (MQ=255)
    gCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg                                                                                                                                             <  1:5045206/150‑1 (MQ=255)
    gCGGAATTCGCGCTGTGGCGGGTTGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCGGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg                                                                                                                                             <  2:1638533/150‑1 (MQ=38)
            cgcgCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgggtaaacc                                                                                                                                     <  2:2125158/150‑7 (MQ=38)
             gcgcTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgggtaaaccc                                                                                                                                    >  1:4434464/1‑143 (MQ=38)
                                       ggAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgg                                                                                                                                            <  2:4129626/116‑1 (MQ=37)
                                       ggAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgg                                                                                                                                            >  1:4129626/1‑116 (MQ=37)
                                                                                                                                  ggtttccccgcGACGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaa               <  1:922105/144‑1 (MQ=255)
                                                                                                                                  ggtttacccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaa               <  1:922153/144‑1 (MQ=255)
                                                                                                                                  ggtttacccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaa               <  1:1958977/144‑1 (MQ=255)
                                                                                                                                  ggtttacccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaa               >  1:1850709/7‑150 (MQ=255)
                                                                                                                                   gtttacccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaac              >  1:2032866/6‑150 (MQ=255)
                                                                                                                                   gtttacccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaac              >  1:2032858/6‑150 (MQ=255)
                                                                                                                                       acccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaacaaca          <  2:1560121/149‑1 (MQ=255)
                                                                                                                                        cccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCATCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCATCCACTCGAAAATAcaacaacaa         >  2:1626156/1‑150 (MQ=255)
                                                                                                                                         gggcgATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTGCGGCAAACACCTCAAAATTCCAACGCCGTCCTCTCAAAATTCCTTCGCCGCCCCCTCAAATTTGCTTCGCCGCCTCCCCTCGAAAATCCATCAACaaa        <  2:1819875/148‑1 (MQ=37)
                                                                                                                                         ccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACTCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCAAACGCCTCCTCCCCTCGAAAATCCAACAACaaa        >  2:3912963/1‑150 (MQ=255)
                                                                                                                                         ccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACaaa        >  2:1364061/1‑150 (MQ=255)
                                                                                                                                          cgcgAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAg       >  2:1600126/1‑150 (MQ=255)
                                                                                                                                           gcgAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTc                                                                            >  2:2368063/1‑80 (MQ=255)
                                                                                                                                           gcgAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTc                                                                            <  1:2368063/80‑1 (MQ=255)
                                                                                                                                           gcgAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGa      >  1:1750202/1‑150 (MQ=255)
                                                                                                                                            tcgaGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGAc     >  2:716552/4‑150 (MQ=255)
                                                                                                                                             gAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGAcc    <  1:625126/150‑1 (MQ=255)
                                                                                                                                              gaGCGCCGCCCGCGCGGCGCTTGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGAccc   >  2:3993354/2‑150 (MQ=255)
                                                                                                                                              aaGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGAccc   >  1:1999148/1‑150 (MQ=255)
                                                                                                                                               aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCt  <  2:3433032/150‑1 (MQ=255)
                                                                                                                                               aGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCt  >  2:3162519/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
ATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCT  >  NC_002947/4808083‑4808375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑