Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,229,239	Δ1 bp	13.7%	intergenic (‑133/+43)	PP_4606 ← / ← PP_4607	ferric siderophore receptor/transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,229,239	0	G	.	13.7%	142.7 / 19.5	51	intergenic (‑133/+43)	PP_4606/PP_4607	ferric siderophore receptor/transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base G (25/19);  new base . (4/3);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

GCGGGGTGAACTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCC  >  NC_002947/5229091‑5229386                                                                                                                                                     |                                                                                                                                                     gcgGGGTGAACTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGc                                                                                                                                                     >  2:746832/1‑150 (MQ=255)      gTGAACTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTTTACCCGcgaaga                                                                                                                                                <  2:14990/150‑6 (MQ=255)               aTGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGtt                                                                                                                                      >  1:816349/1‑150 (MQ=255)                 ggACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTa                                                                                                                                    >  1:936061/1‑150 (MQ=255)                  gACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTCAACCACCTGTGCCTGGAAATATGTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTAc                                                                                                                                   <  1:523079/150‑1 (MQ=255)                  gACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCC‑CCCGTAGGGGCGGGTTTAc                                                                                                                                   <  2:95054/150‑1 (MQ=255)                   aCGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTAcc                                                                                                                                  <  2:1027326/150‑1 (MQ=38)                       aGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCC‑CCCGTAGGGGCGGGTTTa                                                                                                                                    >  2:662404/1‑144 (MQ=39)                         aCCTGTGAGGGGGTTATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGaa                                                                                                                            <  1:1070850/150‑1 (MQ=255)                           cTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAaga                                                                                                                          >  2:159938/1‑150 (MQ=255)                               gAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCt                                                                                                                      <  1:30928/150‑1 (MQ=255)                                aGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGNCTg                                                                                                                     <  2:700172/150‑1 (MQ=255)                                      tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGCCAGCCTCTTCACAGATAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAAGAGGCCGCCACAg                                                                                                               <  1:256766/150‑1 (MQ=18)                                            ttGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAgg                                                                                                                        <  1:132521/135‑1 (MQ=255)                                            ttGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAgg                                                                                                                        >  2:132521/1‑135 (MQ=255)                                            ttGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACt                                                                                                         >  1:901269/1‑150 (MQ=255)                                              gCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTAc                                                                                                       <  1:159938/150‑1 (MQ=255)                                              gCGAAGCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTAc                                                                                                       >  2:187387/1‑150 (MQ=255)                                                gAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTAGCACAGGCAACTACCg                                                                                                     >  2:566734/1‑150 (MQ=255)                                                 aatcaatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTAccaa                                                                                                    >  1:489568/1‑148 (MQ=255)                                                 aatcaatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGa                                                                                                    >  1:27081/1‑150 (MQ=255)                                                           aCTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAgg                                                                                                                        <  1:587112/120‑1 (MQ=255)                                                           aCTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAgg                                                                                                                        >  2:587112/1‑120 (MQ=255)                                                                  cTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAAc                                                                                       >  1:589593/1‑146 (MQ=255)                                                                  cTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAAc                                                                                       <  2:589593/146‑1 (MQ=255)                                                                  cTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGccc                                                                                   >  1:53418/1‑150 (MQ=255)                                                                      ttACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTa                                                                                                        >  2:926943/1‑125 (MQ=255)                                                                      ttACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTa                                                                                                        <  1:926943/125‑1 (MQ=255)                                                                          acGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACgg                                                                           >  1:595366/1‑150 (MQ=255)                                                                              aTGCCACGTGCGGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTg                                                                       <  2:913953/150‑1 (MQ=255)                                                                                  cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGAC‑CAGAGGACCCGTGAAGAGGCTGGCACAGGCAACTCCCCAAGCAGGGTCCACCGCCCCAACACGGGCTGCCGt                                                                   >  1:1093211/1‑150 (MQ=255)                                                                                    cGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGa                                                                 <  1:746832/150‑1 (MQ=255)                                                                                        cTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAgg                                                                                              >  2:687784/1‑117 (MQ=255)                                                                                        cTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAgg                                                                                              <  1:687784/117‑1 (MQ=255)                                                                                                               aaTATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATc                                      <  2:53418/150‑1 (MQ=255)                                                                                                                      ttGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGaa                                        <  2:1126471/141‑1 (MQ=255)                                                                                                                      ttGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGaa                                        >  1:1126471/1‑141 (MQ=255)                                                                                                                        gCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCcag                             <  2:901269/150‑1 (MQ=255)                                                                                                                         cTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCcagc                            >  1:261880/1‑150 (MQ=255)                                                                                                                              gcggcCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCgg                       >  1:220176/1‑150 (MQ=255)                                                                                                                               cagcCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGc                      >  2:199261/3‑150 (MQ=25)                                                                                                                                   ctctTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTc                    >  1:154311/1‑148 (MQ=255)                                                                                                                                   ctctTCGCAGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTc                    <  2:154311/148‑1 (MQ=255)                                                                                                                                   ctctTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCgg                  >  1:164198/1‑150 (MQ=37)                                                                                                                                         acgGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAAGAGGCCGCCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCg            >  1:811843/2‑150 (MQ=25)                                                                                                                                            ggTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGa         >  2:264177/1‑150 (MQ=255)                                                                                                                                              tAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGGTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGaaa       >  1:322949/1‑149 (MQ=255)                                                                                                                                                aaCCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCt     <  2:816349/150‑1 (MQ=255)                                                                                                                                                   ccGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCaaa                                                                                        >  1:286402/1‑64 (MQ=255)                                                                                                                                                   ccGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCaaa                                                                                        <  2:286402/64‑1 (MQ=255)                                                                                                                                                   ccGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGcc  >  2:690318/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GCGGGGTGAACTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCC  >  NC_002947/5229091‑5229386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑