Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,127,712 Δ1 bp 8.7% coding (229/909 nt) PP_5375 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,127,7120A. 8.7% 137.7 / 7.7 46coding (229/909 nt)PP_5375LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (18/24);  new base . (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CAGGTCGATGTTTTCATGGGCACTCTGGAATTCCGACACCTTGGGCAGT‑AACCACAGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACGGCTAATTGCACCCTGGGTAACATTCAATTCCTTG  >  NC_002947/6127563‑6127857
                                                                                                                                                       |                                                                                                                                                 
cAGGTCGATGTTTTCATGGGCACTCTGGAATTCCGACACCTTGGGCAGT‑AACCACAGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCTTATATCGCCGGTGGCGCGAGAAAGGTGAA‑gcatgca                                                                                                                                                   <  1:38409/150‑1 (MQ=255)
                tGGGCACTCTGGAATTCCGACACCTTGGGCAGT‑AACCACAGCGACGCCATGGCGGTATTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCg                                                                                                                                   >  2:593680/1‑150 (MQ=255)
                       tctGGAATTCCGACACCTTGGGCAGT‑AACCACAGCTACGCCATGGCGGGACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACt                                                                                                                            >  2:1030942/1‑150 (MQ=255)
                       tctGGAATTCCGACACCTTGGGCAGT‑AACCACAGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGc                                                                                                                                            <  2:426051/134‑1 (MQ=255)
                       tctGGAATTCCGACACCTTGGGCAGT‑AACCACAGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGc                                                                                                                                            >  1:426051/1‑134 (MQ=255)
                                   acacCTTGGGCAGT‑AACCACAGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTgg                                                                                                                <  1:537277/150‑1 (MQ=255)
                                     acCTTGGGCAGTCAACAACA‑AGCCGACCTGGAGGTTCTGGTAGCAACGGTAAAATGCTGGTTGCCTGGATCCTGGCTGATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTgggg                                                                                                              <  2:1081368/150‑1 (MQ=255)
                                     acCTTGGGCAGT‑AACCACAGCGACGCCATGGCGGTATTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTgggg                                                                                                              <  1:593680/150‑1 (MQ=255)
                                       cTTNGGCAGT‑AACCACAGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGt                                                                                                            >  2:699368/1‑150 (MQ=255)
                                           ggCAGT‑AACCACAGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAgg                                                                                                        <  1:912853/150‑1 (MQ=255)
                                                    ccACAGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGAGCCGGTGGGGGTCAGGGACATGCt                                                                                                >  1:125784/1‑150 (MQ=255)
                                                       caGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACACACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACg                                                                                             >  1:397447/1‑150 (MQ=255)
                                                        aGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACgg                                                                                            <  2:644621/150‑1 (MQ=255)
                                                          cGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGAGCCGGTGGGGGTCAGGGACATGCTACGggt                                                                                          <  2:125784/150‑1 (MQ=255)
                                                                   ggCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGt                                                                                 <  2:82730/150‑1 (MQ=255)
                                                                      ggTACTGGTGGCAACGGTGATTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGTGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGaa                                                                              <  2:564651/150‑1 (MQ=255)
                                                                        tACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATa                                                                            <  1:403553/150‑1 (MQ=255)
                                                                          cTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGt                                                                          <  2:1124676/150‑1 (MQ=255)
                                                                           tggtggCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTg                                                                         <  2:860802/150‑1 (MQ=255)
                                                                             gtggCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAAGTGAA‑GCCTGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTTAGGGACATGCTACGGGTGGTCCGGGTGAATAGttac                                                                       >  2:95716/1‑147 (MQ=255)
                                                                                gCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGt                                                                                 <  1:231867/137‑1 (MQ=255)
                                                                                gCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGt                                                                                 >  2:231867/1‑137 (MQ=255)
                                                                                gCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTg                                                                    >  2:978889/1‑150 (MQ=255)
                                                                                              ttggttgCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCa                                                                >  1:435600/1‑140 (MQ=255)
                                                                                              ttggttgCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCa                                                                <  2:435600/140‑1 (MQ=255)
                                                                                                ggttgCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCaa                                                    <  2:278855/150‑1 (MQ=255)
                                                                                                ggttgCCTTCCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCaa                                                    <  1:110736/150‑1 (MQ=255)
                                                                                                   tgccttgccACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCaagaa                                                 <  1:409673/150‑1 (MQ=255)
                                                                                                     ccttgccACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAgg                                               <  2:107764/150‑1 (MQ=255)
                                                                                                      cttgccACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAATGCATGC‑TTCAcct                                                                                                                                            <  1:950920/56‑3 (MQ=37)
                                                                                                      cttgccACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAATGCATGC‑TTCAcct                                                                                                                                            <  1:951424/56‑3 (MQ=37)
                                                                                                      cttgccACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAATGCATGC‑TTCAcct                                                                                                                                            >  2:951424/1‑54 (MQ=37)
                                                                                                      cttgccACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAATGCATGC‑TTCAcct                                                                                                                                            >  2:950920/1‑54 (MQ=37)
                                                                                                       ttgccACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCg                                             >  2:1057546/1‑150 (MQ=255)
                                                                                                            aCTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTAACGCACCGTTGCGTAATACTGCGCGCCGGTGGGGGACAAGGACATGCTACGGGTGGTACGGGGGAATAGTGCCTTGCCCAGGTAGTGCTCAAGAAGTCGAACCt                                        >  1:1059586/1‑150 (MQ=255)
                                                                                                                gCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTg                                                                    >  2:821584/1‑118 (MQ=255)
                                                                                                                gCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTg                                                                    <  1:821584/118‑1 (MQ=255)
                                                                                                                 cGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACgg                                   <  1:1066622/150‑1 (MQ=255)
                                                                                                                   tataTCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTa                                                                                               >  2:575041/1‑88 (MQ=255)
                                                                                                                   tataTCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTa                                                                                               <  1:575041/88‑1 (MQ=255)
                                                                                                                    tatacGCCAGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATg                                                                                                  >  2:303881/5‑84 (MQ=255)
                                                                                                                    tatacGCCAGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATg                                                                                                  <  1:303881/80‑1 (MQ=255)
                                                                                                                       tCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACGGCTAAtt                             <  1:687900/150‑1 (MQ=255)
                                                                                                                                  gcgGGACAGGTGAA‑GCATGCAGTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACGGCTAATTGCACCCTGGGt                  >  2:620176/1‑150 (MQ=255)
                                                                                                                                       ccAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACGGCTAATTGCACCCTGGGTAACAt             >  1:293685/1‑150 (MQ=255)
                                                                                                                                         aGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACGGCTAATTGCACCCTGGGTAACATTc           >  2:662844/1‑150 (MQ=255)
                                                                                                                                            tGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACGGCTAATTGCACCCTGGGTAACATTCAAt        <  1:978889/150‑1 (MQ=255)
                                                                                                                                                  catgcatTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACGGCTAATTGCACCCTGGGTAACATTCAATTCCtt   <  1:91733/150‑1 (MQ=255)
                                                                                                                                                   atgcatTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGCCGGCTAATTGCACCCTGGGTAACATTCAATTCCTTg  >  1:904399/1‑150 (MQ=255)
                                                                                                                                                   atgcatTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACGGCTAATTGCACCCTGGGTAACATTCAATTCCTTg  <  2:830158/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
CAGGTCGATGTTTTCATGGGCACTCTGGAATTCCGACACCTTGGGCAGT‑AACCACAGCGACGCCATGGCGGTACTGGTGGCAACGGTGACTTGTTGGTTGCCTTGCCACTGGCGTATATCGCCGGTGGCGCGGGCCAGGTGAA‑GCATGCATTCACGCACCGTTTCGTAATACTGCGCGCCGGTGGGGGTCAGGGACATGCTACGGGTGGTACGGGTGAATAGTGCCTTGCCCAGGTAGTCCTCAAGAAGGCGAACCTGACGGCTAATTGCACCCTGGGTAACATTCAATTCCTTG  >  NC_002947/6127563‑6127857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑