| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 702,766:1 | +G | 8.9% | intergenic (+11/‑121) | PP_23SD → / → PP_5SD | 23S ribosomal RNA/5S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 702,766 | 1 | . | G | 8.9% | 165.5 / 15.3 | 56 | intergenic (+11/‑121) | PP_23SD/PP_5SD | 23S ribosomal RNA/5S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base . (28/23); new base G (3/2); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01 | |||||||||||
ACTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAACC > NC_002947/702630‑702914 | aCTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCg > 1:61331/1‑150 (MQ=31)aCTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCg > 1:242291/1‑150 (MQ=31)aCTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGAAAGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCg > 1:869423/1‑150 (MQ=9) gggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCggtg < 1:2958/150‑1 (MQ=32) gggATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAACAATCTGC‑GTAGCAGATTGcttgg < 2:283125/150‑5 (MQ=11) gATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTA‑GCAGATTGcttgggt < 2:711383/150‑7 (MQ=11) gATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCggtggt > 1:498753/1‑150 (MQ=32) cccTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAg > 2:650993/1‑150 (MQ=32) aaGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCg > 1:568818/1‑150 (MQ=32) cGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAAtt > 1:227097/1‑150 (MQ=32) cGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGAACATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGt < 2:278108/150‑1 (MQ=17) gAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTg > 2:860030/1‑150 (MQ=32) gACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCtt < 2:242291/150‑1 (MQ=32) cTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTa < 2:568818/150‑1 (MQ=32) acgacgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTaca < 1:650993/150‑1 (MQ=32) cgacgTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTacac > 1:25962/1‑150 (MQ=32) gATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGAcc > 1:179871/1‑123 (MQ=33) gATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGAcc < 2:179871/123‑1 (MQ=33) gATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCt > 1:828981/1‑150 (MQ=32) gTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACATCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCa > 2:968913/1‑150 (MQ=17) ttGGGTGTGTAAGCGCTGTGCGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCaa > 2:943042/1‑150 (MQ=17) tgtgtAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATc > 2:463261/1‑150 (MQ=32) tgtAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGc < 2:472206/150‑1 (MQ=32) tAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGaa < 2:135667/97‑1 (MQ=34) tAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGaa > 1:135667/1‑97 (MQ=34) gcgcTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATc < 2:498753/150‑1 (MQ=32) tgtgAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTA‑GCAGATTGcttg > 1:420867/1‑81 (MQ=11) tgtgAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGCGTA‑GCAGATTGcttg < 2:420867/84‑4 (MQ=11) tgtgAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATCTCGCATATCCGaa < 2:565293/150‑1 (MQ=17) tgtgAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGaa > 1:714122/1‑150 (MQ=32) tgtgAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGaa > 1:116978/1‑150 (MQ=32) tgAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCCTCGCAAATATCGCATATCCGAAtt < 2:752779/150‑1 (MQ=17) cGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATACGTGCTTTACACAAGAGCTTAGCAAATATCGCATATCCGAATTCGCTg > 1:567037/1‑150 (MQ=17) aCCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCAt > 1:255921/1‑150 (MQ=32) ccAATACTAATTGCCCGTGAGGCTTGACCGTATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATc > 1:1009219/1‑150 (MQ=17) cAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGT‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCt < 2:250819/150‑1 (MQ=17) cAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCt < 2:1129404/150‑1 (MQ=32) tACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGa > 1:442545/1‑150 (MQ=32) aTTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTc < 2:442545/150‑1 (MQ=32) ccGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCa > 2:1090358/1‑150 (MQ=32) cGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCaa > 2:838724/1‑150 (MQ=32) tGACCGTATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTc < 2:1009219/150‑1 (MQ=17) aCCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCtt < 2:20377/150‑1 (MQ=32) ataACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGAACCGAAAATTc > 2:549866/1‑61 (MQ=255) ataACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGAACCGAAAATTc < 1:549866/61‑1 (MQ=255) taACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTgacgac > 2:64669/1‑150 (MQ=32) aaCACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTgg < 1:198056/129‑1 (MQ=33) aaCACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTgg > 2:198056/1‑129 (MQ=33) ccAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATaga < 1:838724/150‑1 (MQ=32) caccgCAATCTGCGTA‑GCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATaga > 1:532048/5‑150 (MQ=11) ccgCAATCTGCGTA‑GCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGc > 1:22417/3‑150 (MQ=2) aaGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGaa < 1:661707/92‑1 (MQ=34) aaGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGaa > 2:661707/1‑92 (MQ=34) aTCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTgg > 2:953114/1‑150 (MQ=32) tCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGa < 2:881489/150‑1 (MQ=32) gC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAacc < 2:61331/150‑1 (MQ=32) c‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAAt > 2:4942/1‑83 (MQ=32) c‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAAt < 1:4942/83‑1 (MQ=32) | ACTGGGATCTTGAATCCCCTAAAGGGCCGTCGAAGACTACGACGTTGATAGGTTGGGTGTGTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCTTGACCATATAACACCCAAGCAATCTGC‑TACGCAGATTGCGGTGGTGAAGACGAAAGACCCGAAAATTCGTGCTTTACACAAGAGCTTCGCAAATATCGCATATCCGAATTCGCTGGGCTGTCCATCTGGACATTCTGGCAACAGAATTTCTTGACGACCATAGAGCATTGGAACC > NC_002947/702630‑702914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |