| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,673,153:1 | +C | 9.3% | coding (424/519 nt) | PP_2340 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,673,153 | 1 | . | C | 9.3% | 153.9 / 11.3 | 43 | coding (424/519 nt) | PP_2340 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (20/19); new base C (2/2); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
TCCGCTTTGTCGAGCAGCAGTCGGCGTATTGCTGGGCTTACCAGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGT > NC_002947/2673004‑2673299 | tCCGCTTTGTCGAGCAGCAGTCGGCGTATTGCTGGGCTTACCAGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGcgatcg > 1:40745/1‑150 (MQ=255) tGTCGAGCAGCAGTCGGCGTATTGCTGGGCTTACCAGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGa < 1:903385/150‑1 (MQ=255) gAGCAGCAGTCGGCGTATTGCTGGGCTTACCATCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGttc < 1:786205/150‑1 (MQ=255) cagcagTCGGCGTATTGCTGGGCTTACCAGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTGTGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGttcttc < 2:688772/150‑1 (MQ=255) cTGGGCTTACCAGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCa < 2:692304/150‑1 (MQ=255) gCTTACCAGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTAc < 1:362313/150‑1 (MQ=255) cAGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGttct > 1:956835/1‑121 (MQ=255) cAGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGttct < 2:956835/121‑1 (MQ=255) aGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGAGCt > 1:368963/1‑150 (MQ=255) gCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCta > 1:381147/1‑150 (MQ=255) ttGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGGTTTCAGAGGCGCACTACCAGCGCTATATCg > 2:536515/1‑150 (MQ=255) gCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGcc < 2:40745/150‑1 (MQ=255) gcgGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCa < 2:936978/150‑1 (MQ=255) gcgGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCt < 2:1045129/150‑1 (MQ=255) tCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATg < 1:209783/150‑1 (MQ=255) cTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATAGCCCCCAGCTTCCTGCGCGATGCCATGGGc > 1:311187/1‑150 (MQ=255) tGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCa < 2:1101029/150‑1 (MQ=255) cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCa > 1:621925/1‑150 (MQ=255) cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCa > 1:929588/1‑150 (MQ=255) tGGTGTTGCTGCCGGAGTTCCGTGAGTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATANCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGt < 1:270266/150‑1 (MQ=255) tgtTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAg > 1:1105493/1‑150 (MQ=255) gctgcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCgg > 1:973041/1‑150 (MQ=255) agcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGGGAGCCGGGc > 1:318327/2‑150 (MQ=255) ccGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGa > 1:944775/1‑150 (MQ=255) ggAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAAt < 2:973041/150‑1 (MQ=255) ggAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGCGCTTCCTGCTTTCACAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCGGCGCGCAGGGCCAGTGAGCCGGGCGaac > 2:715843/1‑149 (MQ=255) gAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATc > 2:196153/1‑150 (MQ=255) ttCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTatat < 1:979576/86‑1 (MQ=255) ttCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTatat > 2:979576/1‑86 (MQ=255) gTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGcc < 2:1105493/150‑1 (MQ=255) tGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATTCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAATCGCCg > 2:155065/1‑150 (MQ=255) aCTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGATGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCg > 1:577606/1‑150 (MQ=255) cTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGCGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGc < 1:1029457/150‑1 (MQ=255) tGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCa > 2:1109680/1‑150 (MQ=255) cccAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCt > 2:586241/1‑78 (MQ=255) cccAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCt < 1:586241/78‑1 (MQ=255) cccAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAAcc > 2:416430/1‑150 (MQ=255) tcgcgAT‑CGATCGCATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATgg < 1:705476/88‑1 (MQ=255) tcgcgAT‑CGATCGCATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATgg > 2:705476/2‑89 (MQ=255) tcgcgAT‑CGATCGCATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgt < 2:939648/130‑1 (MQ=255) tcgcgAT‑CGATCGCATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgt > 1:939648/2‑131 (MQ=255) gcgatgcgatCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCa > 1:616138/1‑150 (MQ=255) cgatgcgatCG‑ATCGTGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAg < 2:1049718/150‑1 (MQ=255) cgatgcgatCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAg < 1:681189/150‑1 (MQ=255) atcn‑atcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt > 2:699873/1‑150 (MQ=255) | TCCGCTTTGTCGAGCAGCAGTCGGCGTATTGCTGGGCTTACCAGCTGATTGCCCGCGGGGCGCGGATGATCCGTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGT > NC_002947/2673004‑2673299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |