Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,941,121:1	+A	8.2%	coding (123/531 nt)	PP_3475 ←	type II secretion system pseudopilin PulG‑like signal peptide protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,941,121	1	.	A	8.2%	227.6 / 13.4	61	coding (123/531 nt)	PP_3475	type II secretion system pseudopilin PulG‑like signal peptide protein
Reads supporting (aligned to +/- strand):  ref base . (29/27);  new base A (3/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GCCTGCGGGTAGCGGTTGTCCTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCG  >  NC_002947/3940975‑3941263                                                                                                                                                    |                                                                                                                                               gCCTGCGGGTAGCGGTTGTCCTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGgcg‑gcg                                                                                                                                             >  2:654659/1‑150 (MQ=255)    tGCGGGTAGCGGTTGTCCTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATCCTCTGTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGGG‑GCGCTc                                                                                                                                          <  1:308239/150‑1 (MQ=255)    tGCGGGTAGCGGTCGTCCTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTc                                                                                                                                          >  2:208932/1‑150 (MQ=255)           aGCGGTTGTCCTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGa                                                                                                                                   <  1:105697/150‑1 (MQ=255)              ggTTGTCCTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCg                                                                                                                                >  2:555400/1‑150 (MQ=255)                   tCCTGTAGCAGGTCGGCCAGATCCTGGGGGTGCTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCg                                                                                                                           >  2:745796/1‑150 (MQ=255)                    ccTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCgg                                                                                                                          >  1:395970/1‑150 (MQ=255)                     cTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCggg                                                                                                                         >  1:382439/1‑149 (MQ=255)                     cTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGt                                                                                                                         >  2:306854/1‑150 (MQ=255)                     cTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGt                                                                                                                         <  1:97521/150‑1 (MQ=255)                      tGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTg                                                                                                                        <  1:208932/150‑1 (MQ=255)                       gTAGCAGGTCGGCCAGATGCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTgg                                                                                                                       >  1:37843/1‑150 (MQ=255)                            aGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGgccgcc                                                                                                                  >  1:114465/1‑150 (MQ=255)                                  gCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGcc                                                                                                            >  2:161657/1‑150 (MQ=255)                                   ccAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCa                                                                                                           >  2:427286/1‑150 (MQ=255)                                     aGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCCAGGCTTGGGGATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGCTGGCCGCCCGGGacaca                                                                                                         >  2:171537/1‑149 (MQ=255)                                     aGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACg                                                                                                         >  2:39256/1‑150 (MQ=255)                                      gATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCGAGCGC‑CGCCCAg                                                                                                                                    >  1:832496/1‑122 (MQ=255)                                      gATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCGAGCGC‑CGCCCAg                                                                                                                                    <  2:832496/122‑1 (MQ=255)                                         ccTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACgctgc                                                                                                     <  1:427286/150‑1 (MQ=255)                                          cTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAg                                                                                                                <  2:726630/138‑1 (MQ=255)                                          cTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAg                                                                                                                >  1:726630/1‑138 (MQ=255)                                          cTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACgctgct                                                                                                    >  2:225435/1‑150 (MQ=255)                                              gggTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTTGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGAcc                                                                                                <  1:977853/150‑1 (MQ=255)                                                gTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGAACGACTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGc                                                                                              >  2:588093/1‑150 (MQ=255)                                                 cccTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCa                                                                                             >  2:671936/3‑150 (MQ=255)                                                   cTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAAt                                                                                           <  2:395970/150‑1 (MQ=255)                                                       gCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATcagc                                                                                       <  1:654659/150‑1 (MQ=255)                                                         cAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATcagcag                                                                                     <  1:555400/150‑1 (MQ=255)                                                               tGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCAc                                                                               >  2:151113/1‑150 (MQ=255)                                                                     ggCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAg                                                                         <  2:359592/150‑1 (MQ=255)                                                                      gcgcGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAgg                                                                        <  1:164592/150‑1 (MQ=255)                                                                                agCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCAAGGTATGTAAAgc                                                              >  1:768114/1‑150 (MQ=255)                                                                                       cAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTAt                                                                     >  1:765906/1‑136 (MQ=255)                                                                                       cAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTAt                                                                     <  2:765906/136‑1 (MQ=255)                                                                                        aaGGCTTGTGCATCCTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTg                                                      <  1:500423/150‑1 (MQ=255)                                                                                         aGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTgg                                                     <  1:225435/150‑1 (MQ=255)                                                                                               gggCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTAc                                               <  1:671936/150‑1 (MQ=255)                                                                                                 gCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCa                                             >  1:584111/1‑150 (MQ=255)                                                                                                       tGGCTGTCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGc                                       >  2:481504/1‑150 (MQ=255)                                                                                                       tGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTTGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAg                                                               >  1:630147/1‑126 (MQ=255)                                                                                                       tGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAg                                                               <  2:630147/126‑1 (MQ=255)                                                                                                           tGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAag                                   >  2:818179/1‑150 (MQ=255)                                                                                                           tGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAag                                   <  1:306854/150‑1 (MQ=255)                                                                                                               aaCCCACAGCAACTGGCGTCCGCGGCCGCGCTGGGCG‑GCCCCCGCCCAGATCGGGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCGGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGc                               <  1:1088860/149‑1 (MQ=255)                                                                                                                aCCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCt                              >  1:498958/1‑150 (MQ=255)                                                                                                                    acaGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGAAGTTCGGCGGACCATTCAGCGAAGAGGCTGCCa                          >  2:529291/1‑150 (MQ=255)                                                                                                                          aCTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCAGTCAGCGAAGAGGCTGCCATCAcgc                    <  1:151113/150‑1 (MQ=255)                                                                                                                             ggCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGcc                 <  1:529291/150‑1 (MQ=255)                                                                                                                             ggCGTGCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGGCGCGATAGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGcc                 <  1:243503/150‑1 (MQ=255)                                                                                                                                gTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACgct                                                                                                       >  2:54934/1‑61 (MQ=255)                                                                                                                                gTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACgct                                                                                                       <  1:54934/61‑1 (MQ=255)                                                                                                                                gTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGccctc              >  2:96722/1‑150 (MQ=255)                                                                                                                                       gTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTgg       <  1:393562/150‑1 (MQ=255)                                                                                                                                       gTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGAAGCAGCACCCCCAGGTATGTAAAGCCACCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTgg       <  2:37843/150‑1 (MQ=255)                                                                                                                                          gcgcTGGGCG‑GCACTCGCCCAGGGCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCAc    <  1:324711/150‑1 (MQ=255)                                                                                                                                          gatcTGGGCGAGCGC‑CGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCa                          <  1:555507/125‑1 (MQ=255)                                                                                                                                          gatcTGGGCGAGCGC‑CGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCa                          >  2:555507/4‑128 (MQ=255)                                                                                                                                            tcTGGGCGAGCGC‑CGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGCTGGGGCTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCg  >  1:618694/2‑150 (MQ=255)                                                                                                                                            gcTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGt                                                                   >  2:601490/1‑85 (MQ=255)                                                                                                                                            gcTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGt                                                                   <  1:601490/85‑1 (MQ=255)                                                                                                                                            gcTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCg  >  2:455189/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                               GCCTGCGGGTAGCGGTTGTCCTGTAGCAGGTCGGCCAGATCCTGGGGGTACTGGGCCAGCCCTGGCGAGGCGCGGTAGTAGCTGCGCAAGGCTTGGGCATACTGGCTGCCGACCCACAGCAACTGGCGTTCGCGGTCGCGCTGGGCG‑GCGCTCGCCCAGATCGTGCCGGTGGCCGCCAGGGCCACGCTGCTGACCGCAATCAGCAGCAGCACCCCCAGGTATGTAAAGCCGCCGTTGGCCTTACCATTCAGCGAAGAGGCTGCCATCACGCGCCCTCCCTGTGGCACCG  >  NC_002947/3940975‑3941263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑